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Citations for Takara Bio :
301 - 350 of 1523 citations for Recombinant Human FCGRT & B2M Protein His Avi Strep II tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... The thrombin-digested recombinant NA was incubated with TALON metal affinity resin (Takara) for 2 h ...
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bioRxiv - Developmental Biology 2021Quote: ... and homogenization (NIM2 buffer supplemented with Recombinant RNase Inhibitor (0.4 U/μL, Takara), SUPERase in (0.2 U/μL ...
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bioRxiv - Plant Biology 2021Quote: ... The recombinant plasmid was transformed into Stellar™ Competent Cells (Clontech, Catalog # 636763) according to the manufacturer’s protocol and positive colonies were selected on LB plates containing kanamycin (50 μg/mL).
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bioRxiv - Pathology 2020Quote: ... followed by treatment with 10 U of recombinant DNase I (Cat.#2270A, TaKaRa) to remove residual DNA ...
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bioRxiv - Cell Biology 2022Quote: ... the MEFs were transduced using a replication defective recombinant retroviral expression system (Clontech) with either wild-type (Parl WT ...
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bioRxiv - Molecular Biology 2022Quote: ... supplemented with 0.1 U/μL of recombinant RNase inhibitor (wash buffer-RIn, Takara). Mechanical cell lysis was achieved by mixing each sample with 0.1 mm glass beads and grinding in a Retsch mixer mill at a frequency of 30/s for 5 min ...
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bioRxiv - Microbiology 2022Quote: ... The recombinant adenovirus was produced using an Adenovirus Dual Expression Kit (Takara Bio) in accordance with the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Extracted RNA (20µg) was treated with recombinant DNase I (RNAse free) (Takara, Japan) and then cleaned-up using RNeasy spin column (Qiagen ...
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bioRxiv - Plant Biology 2023Quote: ... The extracted RNA was treated with Recombinant DNase I (2270A, Takara, Shiga, Japan), and 2 ng equivalent was synthesized using Super Script 3 Supermix (18080400 ...
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bioRxiv - Immunology 2023Quote: ... tubes with 1:1 FBS:PBS supplemented with recombinant RNase inhibitor (1:100, Takara). The live singlet gated CD3+ T cells were further gated as per the gating strategy shown in additional file 1 ...
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bioRxiv - Biophysics 2019Quote: ... Cells were harvested and purified using TALON His-Tag Purification protocol (Clontech Laboratories, Mountain View, CA.) The SUMO tag was cleaved by Ulp-1 as described above and the protein was further purified using ion-exchange chromatography (Bio-Rad ...
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bioRxiv - Plant Biology 2020Quote: ... and on a SD-Leu-Trp-Ade-His plate containing X-α-gal (Takara Bio, USA). Plates were imaged after incubation for 60 - 72 hr at 30°C unless otherwise stated ...
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bioRxiv - Genetics 2022Quote: ... then washed in water and re-suspended in 125-250mL -leu - his galactose liquid culture (Takara Bio Minimal SD Bases ...
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bioRxiv - Molecular Biology 2019Quote: ... the fragment were ligated into the Xho I and Bam HI sites of pEGFP-N3 (Clontech), creating Aβ fused in frame to the N-terminus of GFP ...
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bioRxiv - Neuroscience 2023Quote: ... pAcGFP-His-MAP2C without AcGFP was amplified and Dendra2 was amplified from pDendra2-C vector (Takara). Dendra2 was inserted into where AcGFP was by In-Fusion cloning kit ...
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bioRxiv - Microbiology 2021Quote: ... the cells were transfected with eukaryotic expression plasmid harbouring FLAG or MYC-tagged FBXO22 using Xfect (TAKARA Bio) transfection reagent according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: EGFP-tagged rat PKCδ cDNA were cloned into EcoRI and BamHI digested pRetroX-TetOne-Puro (#634307, Takara Bio) using In-Fusion cloning system (#639648 ...
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bioRxiv - Cell Biology 2023Quote: ... EGFP and mCherry tagged cDNA constructs were cloned into pBMN and modified pLXIN (Clontech, Mountain View, CA, USA) retroviral expression vectors(Peden et al. ...
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bioRxiv - Cell Biology 2023Quote: ... cloned into HA vector using HA tagged hNHE6.2-FL as template and using in-fusion snap method (Takara, In-Fusion® Snap Assembly Master Mix ...
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bioRxiv - Neuroscience 2019Quote: ... and human WT hP-NPO cDNA was amplified from human brain cDNA library (TaKaRa, Cat #637242) with primers ...
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bioRxiv - Cell Biology 2021Quote: ... The nucleotide sequence encoding for human CyPD was obtained from a Human cDNA placenta library (Clontech) by PCR ...
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bioRxiv - Immunology 2019Quote: ... the Sma I site of pT7-pOV8 was changed to Bgl II site with ten mer Bgl II linker (GCAGATCTCG, TAKARA) to produce pT7-Bgl II-pOV8 ...
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bioRxiv - Microbiology 2020Quote: Human embryonic kidney 293T (Clontech, 632180), human lung carcinoma A549 (ATCC ...
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bioRxiv - Molecular Biology 2019Quote: ... with SYBR premix Ex Taq II (Clontech RR820B). ChIP DNA was purified with Qiagen PCR purification kits ...
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bioRxiv - Molecular Biology 2019Quote: ... with SYBR premix Ex Taq II (Clontech RR820B). Standard curves were generated for each primer pair using a serial dilution of cDNA ...
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bioRxiv - Cell Biology 2021Quote: ... and 0.5 µl Reverse Transcriptase Superscript II (Takara). Reverse transcription was carried out in the thermocycler at 42 °C for 90 min ...
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bioRxiv - Plant Biology 2020Quote: ... and SYBR Premix Ex Taq™ II (Takara) was used (Qanmber et al. ...
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bioRxiv - Microbiology 2020Quote: ... with SYBR Premix Ex TaqTM II (TaKaRa, RR820A) and the primers are shown in S3 Table ...
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bioRxiv - Developmental Biology 2022Quote: ... SYBR Green Premix Ex Taq II (Takara, RR820A) was used to analyze the quantitative RT-PCR (RT-qPCR ...
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bioRxiv - Cell Biology 2020Quote: ... and SYBR Premix Ex Taq II (Takara, Japan).
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bioRxiv - Systems Biology 2019Quote: ... with SYBR Premix Ex Taq™ II (Takara). Comparative analysis was performed between the genes of interest normalized by the house keeping genes GAPDH and ACTB ...
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bioRxiv - Plant Biology 2019Quote: ... and SYBR Premix Ex Taq II (TaKaRa Bio), in a CFX96 Real-Time System (Bio-Rad Laboratories) ...
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bioRxiv - Cell Biology 2019Quote: ... with SYBR Premix Ex Taq™ II (Takara). Comparative analysis was performed for the genes of interest ...
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bioRxiv - Evolutionary Biology 2020Quote: ... followed by staining with SYBR Green II (Takara). The fluorescent intensities of the parasitic RNA bands were quantified and the concentrations were determined based on the standard curve drawn with the dilution series of the standard parasitic RNA bands.
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The logic of native enhancer-promoter compatibility and cell-type-specific gene expression variationbioRxiv - Genomics 2022Quote: ... with SYBR® Premix EX TaqTM II (Takara) and primers listed in the table (99-112) ...
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bioRxiv - Genetics 2022Quote: ... cDNA was synthesized using Prime Script II (TAKARA) following the supplier’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... the SYBR Premix Ex Taq II (Takara Bio) was used for quantifying the specific transcripts and analyzed with QuantStudio 6 Flex (Life Technologies) ...
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bioRxiv - Molecular Biology 2022Quote: ... using SYBR® Premix Ex TaqTM II (Takara) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... The SYBR Premix Ex Taq II kit (TaKaRa) was used for qRT-PCR ...
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bioRxiv - Molecular Biology 2023Quote: ... using TB Green Premix Ex TaqTM II (TaKaRa) with the following primers ...
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bioRxiv - Pathology 2023Quote: ... with SYBR Premix Ex TaqTM II (TaKaRa, Japan). The RT-qPCR was performed for at least three biological replicates under the following conditions ...
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bioRxiv - Cell Biology 2023Quote: ... Using TB Green PreMix Ex Tap II (Takara) and specific primers ...
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bioRxiv - Cell Biology 2023Quote: ... using TB Green Premix ExTaq II (Takara Bio) with the following primers ...
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bioRxiv - Microbiology 2020Quote: ... Purification of recombinant Ply was performed using Talon resin (#PT1320-1, Clontech Laboratories Inc.) affinity chromatography from freshly transformed BL21/DE3 E ...
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bioRxiv - Biochemistry 2020Quote: Standard recombinant DNA techniques and In-Fusion® HD Cloning (Takara Bio USA, Inc.) were used for plasmids construction in this study and were verified by DNA sequencing ...
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bioRxiv - Cancer Biology 2023Quote: ... Recombinant lentivirus titer was measured using Lenti-XTM GoStixTM Plus (ClonTech, catalog number: 631280). E0771 and RM-1 cells were transduced with lentiviral vectors carrying the luciferase expression cassette with TransDux MAXTM (System Bioscience ...
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bioRxiv - Microbiology 2023Quote: ... These recombinant plasmids were transformed into Escherichia coli BL21 (Takara Bio Co Ltd, Japan) and the transformed cells were cultured at 37°C until the OD600 reached at 0.6–0.8 ...
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bioRxiv - Molecular Biology 2023Quote: Recombinant AAV9 vectors were generated by co-transfection of AAVpro 293T cells (Takara Bio) with three plasmids ...
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bioRxiv - Biochemistry 2022Quote: ... N-terminal Flag-tagged P38α containing 3C protease site was amplified by PCR using CloneAmp HiFi PCR Premix (Takara) and ligated in to pcDNA3 using the aforementioned restrictions sites ...
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bioRxiv - Microbiology 2022Quote: ... Aer01 tagged with dTomato were labeled by incubating larvae in blocking solution containing Rabbit anti-dsRed (Takara Bio #6324960) at 1:500 dilution ...