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Citations for Takara Bio :
601 - 650 of 4957 citations for Rat Large Proline Rich Protein BAG6 BAG6 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... using the In-Fusion HD Cloning kit (Takara) to generate NanoLuc fusion on the N-terminus of NSD2 ...
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Bni5 tethers myosin-II to septins to enhance retrograde actin flow and the robustness of cytokinesisbioRxiv - Cell Biology 2023Quote: ... using in-fusion cloning kit (Takara, Kusatsu, Japan). To generate pFA6a-link-GBP-CaURA3 ...
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bioRxiv - Genetics 2023Quote: ... and DNA Unique Dual Index Kit (Takara, R400666). Paired-end sequencing of ChIP-seq libraries was performed (150 bp ...
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bioRxiv - Microbiology 2023Quote: ... Protoplasts were stained following ApoAlert kit indications (TaKaRa) and the ratio of apoptotic cells was measured by flow cytometry ...
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bioRxiv - Cancer Biology 2023Quote: ... employing the CalPhos mammalian transfection kit (Takara Bio). Supernatants were harvested at 48 and 72 h post-transfection and subsequently stored at -80°C ...
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bioRxiv - Genomics 2024Quote: ... we repurposed the SMARTer smRNA-seq kit (Takara). First ...
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bioRxiv - Immunology 2023Quote: ... Kit Advantage 2 PCR Enzyme System (Clontech, 639206), QIAquick Gel Extraction ...
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bioRxiv - Molecular Biology 2024Quote: ... using a random primer DNA labeling kit (TaKaRa) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... using the In-Fusion HD Cloning Kit (Clontech) kit ...
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bioRxiv - Immunology 2023Quote: ... using the In-Fusion HD cloning kit (Takara Bio ...
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bioRxiv - Microbiology 2023Quote: ... using the In-Fusion HD Cloning Kit (Takara), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... using the SMARTer smRNA kit for Illumina (Takara), with few modifications ...
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bioRxiv - Microbiology 2024Quote: ... using the In-Fusion HD cloning kit (Clontech). Primers used were OCN334 and OCN430 (ComM) ...
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bioRxiv - Molecular Biology 2023Quote: ... the PrimeScriptTM RT Reagent Kit (Takara Bio Europe) was used ...
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bioRxiv - Cancer Biology 2024Quote: ... the RNA Isolation Kit (RNAiso Plus, Takara, Japan) was employed ...
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bioRxiv - Genomics 2024Quote: SMART-Seq Single Cell PLUS kit (Takara Bio) was used for cDNA library construction ...
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bioRxiv - Plant Biology 2019Quote: ... proteins were transferred to PVDF membrane and blotted proteins were incubated in a 1:10,000 dilution of mouse monoclonal anti-GFP antibody (Living Colors JL-8, Clontech) followed by 1:5000 horseradish peroxidase conjugated sheep anti-mouse IgG antibody (Amersham ...
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A GID E3 ligase assembly ubiquitinates an Rsp5 E3 adaptor and regulates plasma membrane transportersbioRxiv - Cell Biology 2021Quote: ... pGADCg- and pGBKCg-based plasmids containing the indicated protein fusions were transformed into the yeast strain Y2HGOLD (Takara Bio), and double transformants were selected by growth on SD media lacking leucine and tryptophan ...
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bioRxiv - Microbiology 2020Quote: ... lysed by sonication and the recombinant His-tagged protein purified from cell-free extracts using IMAC (Talon resin, Clontech) as previously described19.
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bioRxiv - Microbiology 2019Quote: ... The Pfc43opt recombinant protein was soluble and purified by cobalt affinity chromatography using TALON® metal affinity resin (Takara) under native conditions in phosphate buffered saline (PBS) ...
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bioRxiv - Cell Biology 2021Quote: ... red fluorescent protein fused with the mitochondrial targeting sequence from cytochrome c oxidase subunit VIII (Clontech, Mountain View, CA) using Lipofectamine 2000 (Life Technologies ...
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bioRxiv - Plant Biology 2021Quote: ... NPR1-GFP proteins were detected by reacting the blots with the mouse monoclonal anti-GFP monoclonal antibody (Clontech, USA) and horseradish peroxidase-conjugated secondary antibody (Santa Cruz ...
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bioRxiv - Cell Biology 2020Quote: ... Purification of 6X-Histidine tagged cavin proteins was done using TALON metal affinity resin (ClonTech, Scientifix Cat No. 635503). Talon resin was thoroughly washed with 500GF buffer containing 5 mM imidazole to remove detergent and non-specifically bound proteins ...
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bioRxiv - Biochemistry 2022Quote: The recombinant protein in the soluble fraction was then purified using TALON Metal Affinity Resin (Clontech Laboratories, CA, USA) per manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: Y2H protein interaction assays were performed according to the manufacturer’s instructions as described in the Yeast Protocols Handbook (Clontech; TaKaRa Bio USA ...
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bioRxiv - Molecular Biology 2022Quote: ... red fluorescence protein (DsRed) and NK-NT or NKN1 fragments were cloned into pET6xHN-N Vector (Takara, CA, USA). HEK293T cells were cultured in FP medium (DMEM containing 10% FBS ...
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bioRxiv - Molecular Biology 2023Quote: ... Equal loading of the GFP tagged E2F1 proteins was determined by the GFP antibody (Clontech, lot. 1404005; 1:2000) the secondary antibody anti-rabbit HRP (Na934v ...
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bioRxiv - Cancer Biology 2023Quote: ... His-TEV tagged protein was captured with Co-TALON resin (Clonetech, Takara Bio USA, 2 mL slurry/liter culture) at 4 ºC for 1 h with constant end-to-end mixing ...
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bioRxiv - Microbiology 2023Quote: ... The protein-bead mixtures were then loaded onto a gravity column (TALON 2 mL Disposable Gravity Column, Takara #635606). Lysate was loaded into the column 5 times with gravity flow and then washed in a 1M NaCl buffer 3 times ...
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bioRxiv - Biochemistry 2024Quote: ... The other 2A proteins were separated from the lysate using immobilized metal affinity chromatography with either TALON beads (Clontech) or chelating Sepharose beads charged with NiCl2 (Cytiva) ...
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bioRxiv - Plant Biology 2020Quote: The total RNA of the buds collected for the transcriptome research was extracted by the EASYspin Plus Plant RNA Kit RN37 (Aidlab Biotechnology) and cDNA synthesis by the M-MLV RTase cDNA Synthesis Kit (TaKaRa Company). cDNA amplified fragment length polymorphism (cDNA-AFLP ...
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bioRxiv - Neuroscience 2021Quote: Total RNA from DRG was extracted using RNeasy micro kit and reverse-transcribed using PrimeScript RT Reagent Kit (Cat# RR037A, TaKaRa, Japan). Real-time PCR was performed using the SYBR Premix Ex Taq™ (Cat# DRR041A ...
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bioRxiv - Biochemistry 2019Quote: ... cDNAs were synthesized from the isolated RNA using a cDNA synthesis kit (Advantage RT-PCR kits) from Clontech (Mountain View, CA). Specific primers designed according to human ...
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bioRxiv - Cancer Biology 2022Quote: Total RNA was extracted from cells by TRIzol and an RNA extraction kit (MiniBEST Universal RNA Extraction Kit, RR820A, Takara, China). Total RNA (1 μg ...
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bioRxiv - Genetics 2020Quote: RNA was extracted using the Biostic Blood Total RNA Isolation Kit sample (MO BIO laboratories, Inc) and cDNA was obtained using the PrimeScript™RT reagent Kit (Takara). RTqPCR gene expression analysis was performed using the Taqman probes HSF2-Hs00988309_g1 and GADPH-Hs02758991_g1 for normalization (Applied Biosystems).
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Engineering of membrane complex sphingolipids improves osmotic tolerance of Saccharomyces cerevisiaebioRxiv - Bioengineering 2019Quote: Total RNA was extracted using MiniBEST universal RNA extraction kit and 1 μg was taken to synthesize cDNA using the PrimeScript II 1st-strand cDNA synthesis kit (TaKaRa, Japan). The cDNA mixture was diluted to about 100 ng/μl and used as the template for the gene expression level analysis by qRT-PCR ...
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bioRxiv - Physiology 2021Quote: ChIP-seq libraries were prepared on 5 ng of DNA from ChIP and input samples using the SMARTer ThruPlex DNA-seq kit along with DNA HT Dual Index Kit (both from Takara Bio) according the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: Viral RNA was extracted directly from the virus stock using the Indimag Pathogen kit (Indical Biosciences) and transcribed to cDNA using the PrimeScript™ RT reagent Kit (Takara) using oligo-dT and random hexamers ...
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bioRxiv - Microbiology 2021Quote: ... Plasmid DNA was isolated using the FastGene Plasmid Mini Kit (Nippon Genetics, Tokyo, Japan) or NucleoSpin Plasmid EasyPure Kit (Takara Bio). The REase NdeI was employed for the linearization of plasmid DNAs ...
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bioRxiv - Pathology 2023Quote: ... Whole genome amplification was performed on the prepared mixture samples with BioSkryb ResolveDNA™ Whole Genome Amplification Kit (BioSkryb Genomics) and Takara PicoPlex WGA v3 kit (Takara) following the manufacture’s protocols ...
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bioRxiv - Genomics 2023Quote: ... A total of 3.5 μl ribo-depleted material was used for SMARTer (SMARTer PCR cDNA Synthesis Kit, catalog num. 634926 and Advantage 2 PCR kit, catalog num. 639207, Clontech-Takara) protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... and CDS of fluorescent proteins were subcloned into pET-human αSyn51 using the KOD-Plus Mutagenesis kit (TOYOBO) and In-Fusion HD Cloning Kit (Takara Bio) according to the manufacturers’ protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... The viral particles were purified from transfected cells and titrated using the AAVpro Purification Kit Maxi and AAVpro Titration Kit (Takara Bio) according to the manufacturer’s instructions.
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bioRxiv - Physiology 2024Quote: cDNA synthesis and Real-Time PCR were performed following the manufacture instructions of the PrimeScript RT reagent Kit with gDNA Eraser kit (Takara, #RR047A). For the Real-Time PCR reaction ...
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bioRxiv - Neuroscience 2024Quote: Total RNAs from scramble shRNA or TDP-43 shRNA treated cells were used to construct RNA-sequencing libraries using the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian kit (Takara 634411), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... RNA was extracted with an Axygen RNA purification kit (Capital Scientific) and the first DNA was synthesized from RNA using a PrimeScript RT regent kit (Takara, Shiga, Japan). Actin-coding gene (KP341672 ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA was removed using the DNA-free kit (Thermo Fischer Scientific, Cat# AMl906) and subsequent cDNA synthesis was performed using the PrimeScript RT reagent Kit (TAKARA, Cat# RR037A), both according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was collected using the Nucleospin RNA Kit (Macherey–Nagel, Hoerdt, France) and transcribed into complementary DNA (cDNA) with the PrimeScript RT Reagent Kit (RR037A, Takara, Kusatsu, Japan). cDNA was then amplified with the SYBR Green Master kit (Roche ...
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The MKK3 MAPK cascade integrates temperature and after-ripening signals to modulate seed germinationbioRxiv - Plant Biology 2024Quote: ... RNA was reverse-transcribed to cDNA by using DNA removal and a cDNA synthesis kit (PrimeScriptTM RT reagent Kit with gDNA Eraser; TaKaRa, Kusatsu, Japan) with a mixture of oligo-dT and random primers ...
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bioRxiv - Cancer Biology 2021Quote: RNA was extracted using the nucleospin RNA kit (Takara) according to the manufacturer’s recommended protocol ...