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Citations for Takara Bio :
251 - 300 of 5049 citations for QuantiFluo Protein Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Diversified expression of gamma-protocadherins regulates synaptic specificity in the mouse neocortexbioRxiv - Neuroscience 2021Quote: LATaq Kit (RR002B, TaKaRa) was used in nested PCR ...
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bioRxiv - Cancer Biology 2022Quote: ... 3’RACE kit (Takara) was used to convert RNAs of the prostate tumors into cDNAs by a reverse transcriptase and oligo-dT adapter primer ...
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bioRxiv - Biophysics 2019Quote: ... the detergent-solubilised protein were batch bound to Co2+-charged Talon resin (Clontech) by gentle rotation at 4 °C for 1 h ...
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bioRxiv - Systems Biology 2021Quote: ... the inducible fluorescent protein was induced by adding 1 μg/ml doxycycline (Clontech) alone or together with 100 nM rapamycin (Harveybio ...
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bioRxiv - Neuroscience 2021Quote: ... the fluorescent proteins were amplified from pAM FLEX eGFP and pmCherry-C1 (Clontech) respectively ...
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bioRxiv - Cell Biology 2021Quote: ... 6xHis tagged proteins were purified using Talon Co2+ affinity resin (Takara Bio, USA) and eluted with 150 mM Imidazole in the lysis buffer pH 7.8 ...
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bioRxiv - Microbiology 2021Quote: ... The protein bands were visualized using the Western BLoT Hyper HRP Substrate (TAKARA) and exposed using a Chemiluminescence Imaging System (Fusion Solo S ...
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bioRxiv - Cell Biology 2022Quote: Every protein expressed in mouse rods was also cloned into pEGFP-N1 (Clontech) for expression in AD293 cells using the AgeI and NotI cloning sites within the vector to replace EGFP with the tagged proteins ...
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bioRxiv - Plant Biology 2020Quote: ... Transferred proteins were first probed with anti-GFP (Takara Bio, 1:5,000 dilution) for 16 h ...
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bioRxiv - Biochemistry 2019Quote: ... the Rab8A proteins were co-expressed with the chaperone GroEL/S (pGro7, Takara). The expression of the GroEL/S chaperone was auto-induced by supplementing the LB-medium with 1 mg/mL arabinose.
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bioRxiv - Biochemistry 2021Quote: ... Proteins in the supernatant were purified by the affinity chromatography with TALON (Clontech) resin ...
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bioRxiv - Cell Biology 2020Quote: ... Shield-1 ligand for stabilization of DD-tagged proteins was purchased from Takara Bio (Cat # 632189 ...
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bioRxiv - Molecular Biology 2023Quote: ... Monoclonal U-2OS cell lines stably expressing Tet-On 3G transactivator protein (Clontech), a tandem-dimeric MS2 hairpin binding protein tagged with eYFP (MS2CP-YFP) ...
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bioRxiv - Cell Biology 2023Quote: For mammalian expression of eGFP labelled proteins we used the pEGFP-C1 (Clontech) vector ...
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bioRxiv - Developmental Biology 2023Quote: ... which was induced for protein purification using Talon Metal Affinity Resin (Takara 635501). Two New Zealand white rabbits were injected at ten sites with 500μl of Freund’s Complete Adjuvant (Sigma-Aldrich F5881 ...
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bioRxiv - Biochemistry 2023Quote: ... MA) and the protein was purified using Talon beads (Takara Bio Inc., Japan) following published protocols 37,38 ...
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bioRxiv - Cell Biology 2023Quote: ... for GST-tagged protein or on TALON-Cobalt beads column (635507, Takara Bio) for 6XHIS-tagged protein as previously described [19,26] ...
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bioRxiv - Neuroscience 2023Quote: ... the fluorescent proteins were amplified from pAM FLEX eGFP and pmCherry-C1 (Clontech) respectively ...
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bioRxiv - Plant Biology 2024Quote: ... The JMJ27-GFP fusion protein was detected using the anti-GFP (Takara: 632593) 1:2,000 (v ...
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bioRxiv - Microbiology 2024Quote: ... The fusion protein was affinity-purified using TALON metal affinity resin (Takara Biosciences) and eluted in buffer containing 50 mM Tris-HCl ...
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bioRxiv - Plant Biology 2020Quote: ... β-galactosidase assay was performed according to the manufacturer’s protocol (Matchmaker Two-Hybrid System; Takara, https://www.takarabio.com).
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bioRxiv - Plant Biology 2021Quote: The Yeast two-hybrid assay was performed using the Matchmaker™ Gold Yeast Two-Hybrid System (Clontech). The full-length coding region of ZmBZR1 were ligated into pGBKT7 plasmid as bait and the full-length coding regions of ZmBEH1 was fused into pGADT7 vector as prey ...
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bioRxiv - Plant Biology 2021Quote: ... The Y2H assays were performed following the manufacturer’s protocol using the Matchmaker GAL4 Two-Hybrid System (Clontech). Clones were grown on selective dropout medium (lacking either Trp and Leu or Trp ...
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bioRxiv - Plant Biology 2021Quote: ... yeast one-hybrid assays were performed as the manual of Matchmaker Gold Yeast One-Hybrid System (Clontech). The coding sequence of BnaA03.WRKY28 was cloned into pGADT7 vector ...
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bioRxiv - Molecular Biology 2019Quote: ... Quantitative PCR assays were performed on a qTOWER apparatus (Analytic Jena) using SYBR Green master mix (TaKaRa). Gene expression was normalized for the expression of 36B4 ...
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bioRxiv - Microbiology 2020Quote: ... The purified virus was titrated with Lenti-X p24 Rapid Titer Assay (Takara Bio, Cat. No. 632200). The virus was stored at -80 °C for future use.
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bioRxiv - Cell Biology 2019Quote: ... Yeast two-hybrid assays were performed according to the Matchmaker Gold Yeast two-hybrid system manual (Clontech). Different combinations of constructs were co-transformed into yeast strain AH109 using the polyethylene glycol/lithium acetate method as described in the manual of Clontech ...
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bioRxiv - Biochemistry 2021Quote: A portion of the viral supernatant collected for infectivity assays was concentrated using the Retro-X (Clontech) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: Yeast two-hybrid assays were performed in the yeast strain Y2Hgold with Matchmaker two-hybrid system (Clontech) according to manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2022Quote: The yeast two hybrid assay was performed as indicated in MATCHMAKER GAL4 two-hybrid system 3 (Clontech). The protein coding regions of genes used in this study were amplified from Guy11 cDNA with primer pairs listed in Table 1.1 ...
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bioRxiv - Cell Biology 2022Quote: ... cereviseae strains Y2HGold and Y187 were used for Yeast Two Hybrid assay (Takara Bio, Mountain View, USA).
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bioRxiv - Molecular Biology 2023Quote: ... Y2H assay was performed by the manufacturer’s protocol of the Matchmaker GAL4 Two-hybrid System (Clontech, USA). The prey and bait were cotransformed into Saccharomyces cerevisiae strain AH109 ...
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bioRxiv - Plant Biology 2024Quote: Y2H assay was performed following the Matchmaker® Gold Yeast Two-Hybrid System User Manual (Takara Bio). Briefly ...
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bioRxiv - Cell Biology 2023Quote: The yeast two-hybrid assay was performed according to the manufacturer’s protocol (Clontech MATCHMAKER System Manual, Clontech). Plasmids harboring Bqt4 or Bqt3 were co-transfected into Saccharomyces cerevisiae AH109 strain ...
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bioRxiv - Cell Biology 2023Quote: The yeast two-hybrid assay was performed according to the manufacturer’s protocol (Clontech MATCHMAKER System Manual, Clontech). Plasmids harboring Bqt4 or Bqt3 were co-transfected into Saccharomyces cerevisiae AH109 strain ...
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Effects of Sanhuang plaster on expression of MyD88, TRAF6, MIP-1β and IL-23 in rats infected by MRSAbioRxiv - Molecular Biology 2022Quote: ... Real-time fluorescence quantitative PCR kit and reverse transcription kit(TaKaRa Co., Ltd.); MyD88 Anti-body TRAF6 Anti-body MIP-1β Anti-body and IL-23 Anti-body (GeneTexCo. ...
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bioRxiv - Biophysics 2020Quote: ... using a cDNA synthesis kit (PrimeScript 1st strand cDNA Synthesis Kit, Takara Bio), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Adeno-X Maxi Purification Kit and Adeno-X Rapid Titer Kit from Clontech were used for purification and titration ...
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bioRxiv - Cancer Biology 2023Quote: ... Adeno-X Maxi Purification Kit and Adeno-X Rapid Titer Kit from Clontech were used ...
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bioRxiv - Plant Biology 2019Quote: Yeast two-hybrid assays were carried out using the Matchmaker™ gold Yeast two-Hybrid Systems (Clontech, USA). Different truncated CDS of EcaICE1 without transcriptional activation activity were subcloned into pGBKT7 (BD ...
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bioRxiv - Plant Biology 2022Quote: The Y2H assays were performed using the Matchmaker Gold Yeast Two-Hybrid System (Clontech, Mountain View, CA, USA). The full-length and truncated coding regions of WL1 were amplified and cloned into the yeast expression vector pGADT7 (Clontech ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were lysed and quantitative β-galactosidase assay was performed using ONPG substrate following standard protocols (Clontech Laboratories). For two-hybrid experiments in meiotic conditions ...
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bioRxiv - Cell Biology 2020Quote: Yeast two-hybrid assays were performed according to the manual of Matchmaker Gold Yeast two-hybrid system (Clontech). The relevant combinations of constructs were co-transformed into yeast strain AH109 using the polyethylene glycol/lithium acetate method as described in the manual ...
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bioRxiv - Biochemistry 2021Quote: ... All yeast two-hybrid screens and assays were performed using the BD Matchmaker system (BD Biosciences, Clontech Laboratories) utilizing the Saccharomyces cerevisiae strain AH109 essentially as described earlier (Stephan et al. ...
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bioRxiv - Plant Biology 2021Quote: ... and used as a template for qPCR assays employing the SYBR® Premix Ex Taq™ II (Takara) with primers listed in Table S1 ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were lysed and quantitative β-galactosidase assay was performed using ONPG substrate following standard protocols (Clontech Laboratories).
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bioRxiv - Plant Biology 2022Quote: ... For Y2H assays, cDNAs of mCherry and ZmSIPs (ZmSIP1, ZmSIP2, ZmSIP3) were cloned into the pGADT7 vector (Clontech) for expression via the GAL4 AD under constitutive ADH1 promoter ...
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bioRxiv - Cancer Biology 2024Quote: ... we employed the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) or WST-1 (Water-Soluble Tetrazolium 1) assay from Takara, Japan ...
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bioRxiv - Cell Biology 2020Quote: ... HIS-Ub-conjugated proteins were purified by cobalt chromatography (TALON metal affinity resin, Clontech), as described in the manual ...
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bioRxiv - Biophysics 2019Quote: ... 300 ng of the plasmid encoding the green fluorescent protein (pEGFP, Clontech, CA, USA) and 12 μL of Lipofectamine/Plus reagent ...