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Citations for Takara Bio :
51 - 100 of 4764 citations for QuantiChrom Hemoglobin Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Protein concentrations were determined via the Bradford method using the TaKaRa Bradford Protein Assay Kit (Takara Bio, T9310A).
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bioRxiv - Microbiology 2022Quote: ... a one-step reverse transcription-PCR (RT-PCR) assay was conducted with a PrimeScript kit (Takara, Tokyo, Japan), and Viral genomic terminal sequences were determined using commercial 5’ and 3’ RACE (rapid amplification of cDNA ends ...
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bioRxiv - Physiology 2022Quote: ... Media lactate dehydrogenase (LDH) was assayed by enzymatic colorimetric assay (LDH Cytotoxicity Detection Kit, Takara Bio, cat# MK401).
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bioRxiv - Immunology 2021Quote: ... was used to for two-step qPCR assay after cDNA synthesis using PrimeScript® reverse transcriptase kit (Takara, Japan). Gene expression was normalized to the expression level of β-actin ...
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Guidelines for accurate genotyping of SARS-CoV-2 using amplicon-based sequencing of clinical samplesbioRxiv - Genomics 2020Quote: ... CDC-USA assay targeting gene N (IDT # 10006713) and One Step PrimeScript™ III RT-PCR Kit (TaKaRa #RR600A). Serial dilutions of reference material were prepared ranging from 1 to ~10M genome equivalents per reaction ...
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bioRxiv - Neuroscience 2023Quote: TUNEL assays were performed to detect apoptotic cell death using Clonetech ApoAlert DNA Fragmentation kit (Takara, Kusatsu, Shiga, Japan) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... The Y2H assay was carried out based with the manufacturer’s protocol of Matchmaker Gold Yeast Two-Hybrid System Kit (Takara). The primers used for vector construction are listed in Supplemental Data Set S1.
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PPARγ is a tumor suppressor in basal bladder tumors offering new potential therapeutic opportunitiesbioRxiv - Cancer Biology 2019Quote: ... The GAL4 DNA-binding domain cloning vector pM and the activation-domain cloning vector pVP16 are part of the Mammalian Matchmaker Two-Hybrid Assay kit (BD Biosciences Clontech). The construct pM-MED1 (510-787 ...
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bioRxiv - Microbiology 2020Quote: ... according to the manufacturer’s instructions and detected by RT-qPCR assays with a One-Step PrimeScript RT-PCR kit (Takara, Japan) using SARS-CoV-2-specific primers on an Applied Biosystems 7500 Real-time PCR System.
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bioRxiv - Immunology 2022Quote: ... RNA concentrations were determined using the Qubit high-sensitivity RNA assay and small RNA libraries were created using the Clontech SMARTer smRNA-Seq preparation kit according to manufacturer instructions (Takara). Small RNA inserts were further enriched using AMPure bead selection (Beckman Coulter ...
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bioRxiv - Cell Biology 2023Quote: ... pH 7.4) and the protein contents of the cell lysate were quantified using a BCA Protein Assay Kit (TAKARA, T9300A) following the manufacturer’s protocol (Song et al. ...
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bioRxiv - Synthetic Biology 2023Quote: ... SEAP activity in supernatant (25 μL) was measured using the Great EscAPe SEAP Chemiluminescence Assay kit 2.0 (Clontech, Fremonet, CA). The GLuc activity in supernatant (20 μL ...
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bioRxiv - Cell Biology 2023Quote: ... Supernatants were collected and mixed with Laemmli buffer after measuring the protein concentration using a TaKaRa BCA Protein Assay Kit (TaKaRa). Samples were subjected to SDS-polyacrylamide gel electrophoresis and electrotransferred onto polyvinylidene difluoride membranes ...
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bioRxiv - Molecular Biology 2024Quote: ... Final cDNA samples were used then used for quantitative real-time PCR assay by SYBR Green PCR Kit (RR820A, Takara). Quantitative primers were designed based on the gene sequences by Sangon Biotech ...
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bioRxiv - Physiology 2023Quote: The WST-1 assay (Takara) was performed on HepG2 cells and Huh7 cells to assess the cellular cytotoxicity of Tecomella undulata ...
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bioRxiv - Plant Biology 2020Quote: ... Yeast two-hybrid assay and one-hybrid assay were performed following the manufacturer’s instructions (Clontech).
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bioRxiv - Plant Biology 2020Quote: ... The yeast one-hybrid (Y1H) assay was conducted using the Matchmaker™ Gold Yeast One-Hybrid Library Screening System kit (Cat. no. 630491, Clontech).
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bioRxiv - Developmental Biology 2020Quote: ... TUNEL assay was performed to detect apoptotic cell death using the in situ Apoptosis Detection Kit (Takara Bio Inc., Shiga, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... The Y2H assay was performed following the manual provided in the kit “Matchmaker Gold Yeast Two-Hybrid” (Clontech, Mountain View, USA). The list of VirE2 interacting clones has been given in Fig.S1.
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bioRxiv - Immunology 2021Quote: ... The transcripts of the DEMs and DEPs were analyzed and validated by qRT-PCR assays using the Mir-X miRNA qRT-PCR SYBR® Kit (TaKaRa, Japan) and the PrimeScriptTM RT reagent Kit (TaKaRa ...
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bioRxiv - Immunology 2021Quote: ... and the primers of the U6 snRNA used in the qRT-PCR assays were provided in the Mir-X miRNA qRT-PCR SYBR® Kit (Takara, Japan). The qRT-PCR data were analyzed by the 2−ΔΔCt method as described in Livak & Schmittgen (2001 ...
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bioRxiv - Microbiology 2023Quote: The 5’ and 3’ termini of the sRNA OueS were determined by the RACE assay (SMARTer RACE 5’/3’ kit, Takara Bio USA), adapted for non-poly-A-tailed RNA ...
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bioRxiv - Microbiology 2023Quote: ... levels were measured by a qRT-PCR assay using the One Step TB Green PrimeScript PLUS RT-PCR Kit (Perfect Real Time) (TaKaRa, Cat# RR096A). The PCR protocol was 42°C for 5 min ...
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bioRxiv - Microbiology 2023Quote: ... vRNA copy numbers of each virus stock were measured via RT-qPCR assay with the One Step TB Green PrimeScript PLUS RT-PCR Kit (Perfect Real Time) (TaKaRa, Cat# RR096A) as described previously [12] ...
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bioRxiv - Immunology 2023Quote: ... which was measured using a real-time PCR assay with a SARS-CoV-2 direct detection RT‒qPCR kit (Takara Bio, Siga, Japan). The IC50 was calculated by IC50 Calculator (https://www.aatbio.com/tools/ic50-calculator ...
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bioRxiv - Cell Biology 2023Quote: ... A filter-based β-galactosidase assay and liquid culture assay using ONPG were performed in three replicates as described in the Yeast protocols handbook (Clontech). As a positive control for the study ...
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bioRxiv - Biochemistry 2020Quote: ... Protein concentration was determined by Bradford assay (TAKARA). Nickel affinity purification of 10His-SUMO1T95R conjugates was carried out with Ni-NTA agarose beads (Qiagen) ...
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bioRxiv - Microbiology 2020Quote: ... For cell death measurement by LDH assay (Takara), culture supernatants were clarified with a 5 minute spin at 400×g ...
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bioRxiv - Microbiology 2019Quote: ... the WST-1 Cell Proliferation Assay System (TaKaRa) was used following the manufacturer’s recommendation ...
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bioRxiv - Bioengineering 2019Quote: ... The luciferase assay was conducted according to a standard protocol of Ready-To-Grow Dual Secreted Reporter Assay (Clontech Laboratories, Inc., US) except that the amount of substrate was reduced to half of the defined amount.
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bioRxiv - Cancer Biology 2019Quote: ... The assay was performed following manufacturer’s protocol (Takara, Japan) and relative cytotoxicity was calculated according to the same protocol.
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bioRxiv - Immunology 2020Quote: ... The protein concentration was determined by Bradford assay (Takara), and the cell lysate was mixed with 4x Laemmli loading buffer containing β mercapto-ethanol ...
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bioRxiv - Genomics 2024Quote: ... and titer quantified using p24 ELISA antigen assay (Takara). MOI=5 was used to transduce 1x1097 EndoC-βH3 cells in culture media without pen/strep and puromycin.
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bioRxiv - Plant Biology 2019Quote: ... Y2H assays were performed using the Matchmaker 3 system (Clontech). The resulting transformants with appropriate positive and negative controls were spotted on SD (-Leu/-Trp ...
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bioRxiv - Cancer Biology 2020Quote: ... WST-1 assays were performed as per manufacturer’s instructions (Takara) (18).
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bioRxiv - Plant Biology 2022Quote: Y2H assays were performed according to the manufacturer’s protocol (Clontech). For Y2H screening ...
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bioRxiv - Plant Biology 2023Quote: Yeast two-hybrid assays were performed using strain AH109 (Takara). Vectors pGADT7 and pGBKT7 were sequentially transformed into this strain ...
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bioRxiv - Synthetic Biology 2021Quote: ... Pseudotyped viruses were quantified with a p24 assay (Takara Bio #632200) per manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... Y2H assay was performed according to the Yeast Protocols Handbook (Clontech) using the Y2H Gold yeast reporter strain (Clontech) ...
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bioRxiv - Plant Biology 2023Quote: Y1H assay was conducted according to protocol (Clontech, cat. No. 630491). Briefly ...
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bioRxiv - Plant Biology 2023Quote: Y2H assay was performed according to protocol (Clontech, cat. No. 630489). Since BcICE1 self-activates ...
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bioRxiv - Plant Biology 2023Quote: Y2H assays were performed using the MatchmakerTM Two-Hybrid System (Clontech). Bait (pGBKT7 ...
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bioRxiv - Microbiology 2023Quote: ... SEAP activity was quantified using the Great EscAPe SEAP assay (Clontech). Fluorescence was read on a Filtermax F5 plate reader (Molecular Devices).
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bioRxiv - Plant Biology 2024Quote: ... Yeast two-hybrid assays were performed using yeast strain AH109 (Clontech) following standard procedures (Yeast Protocols Handbook PT3024-1 ...
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bioRxiv - Plant Biology 2021Quote: Cell viability assays were performed by using either Y2HGold yeast strains (Clontech) containing a pBridge bait vector or diploid yeast strains generated by mating a Y2HGold strain containing a bait vector with a Y187 strain (Clontech ...
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bioRxiv - Plant Biology 2021Quote: Y2H assays were performed using the Matchmaker™ Two-Hybrid System (Clontech). Bait (pGBKT7 ...
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bioRxiv - Plant Biology 2021Quote: Y2H assays were performed using the Matchmaker GAL4 two-hybrid system (Clontech). The full-length and/or partial (RING ...
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bioRxiv - Immunology 2020Quote: ... The PCR assays were run using LA Taq polymerase (Takara Bio, TAKRR002) with the following thermocycling conditions ...
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bioRxiv - Cancer Biology 2023Quote: ... 10 µL of premix WST-1 cell proliferation assay (Takara Bio, Inc.) was added to each well and the plate was incubated for an additional 2 hours ...
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bioRxiv - Plant Biology 2023Quote: ... Quantitative α-galactosidase assay was also performed according to manufactural instruction (Clontech). Briefly ...