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Citations for Takara Bio :
1 - 50 of 815 citations for Piggybac Transposable Element Derived 3 PGBD3 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... The plasmid for G3BP1-eGFP expression was cloned starting from an ePB-bsd-Eif1a (PiggyBac transposable vector) backbone (47) using sequential In-Fusion Cloning (Takara Bio Inc., Otsu, Japan) reactions ...
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bioRxiv - Cancer Biology 2020Quote: ... a targeting vector was constructed (Supplementary Figure 1) to include a heptameric tetracycline-response element (TRE) derived from the pTRE2 vector (Clontech), inserted into the intron 2 of the endogenous Myc locus that serves as a binding site for tetracycline-regulatable transcriptional repressors ...
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bioRxiv - Cell Biology 2020Quote: ... pC22 was derived from Clontech vector pEGFP-C2 by cutting with EcoRI plus XmaI ...
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bioRxiv - Neuroscience 2024Quote: EGFP derived from pEGFP-C1 (Clontech) was cloned in-frame to the N-terminal domain of the mouse NFL gene (derived from pmNFL ...
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bioRxiv - Biochemistry 2021Quote: ... 1 μg of PB-Tet-On Advanced (containing PiggyBac Transposon sequences57 flanking rtTA-Advanced (Takara Bio) and puromycin selection cassette) ...
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bioRxiv - Molecular Biology 2023Quote: ... along with the hyperactive PiggyBac Transposase (hyPBase) utilizing Xfect transfection reagent (Takara-Bio, San Jose, California). Transposase and Transposon DNA were added at 1:5 ratio in Xfect transfection reagent per manufacturer instructions to form the nanoparticle transfection complex ...
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bioRxiv - Biochemistry 2021Quote: ... plasmid backbones derived from EYFP-N1 (Clontech/Takara), Lenti-Cas9-blast (a kind gift of F ...
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bioRxiv - Biochemistry 2021Quote: ... plasmid backbones derived from EYFP-N1 (Clontech/Takara), Lenti-Cas9-blast (a kind gift of F ...
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bioRxiv - Biochemistry 2021Quote: ... which is derived from the pBacPAK8 system (ClonTech). Recombinant baculoviruses were prepared in Spodoptera frugiperda (Sf9 ...
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bioRxiv - Cell Biology 2023Quote: ... plasmid backbones derived from EYFP-N1 (Clontech/Takara), Lenti-Cas9-blast (a kind gift of F ...
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bioRxiv - Cell Biology 2023Quote: ... plasmid backbones derived from EYFP-N1 (Clontech/Takara), Lenti-Cas9-blast (a kind gift of F ...
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bioRxiv - Cell Biology 2022Quote: ... which is derived from the pBacPAK8 system (ClonTech). CUL4A (FL ...
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bioRxiv - Microbiology 2024Quote: ... Retroviral expression vectors were derived from pQCXIH (Clontech) and lentiviral vectors were derived from pTM952 ...
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bioRxiv - Cell Biology 2021Quote: ... Previously built plasmids derived from pmCherry-N1 (Takara-Clontech) and piRFP (a gift from Michael Davidson & Vladislav Verkhusha ...
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bioRxiv - Cell Biology 2021Quote: ... Previously built plasmids derived from pmCherry-N1 (Takara-Clontech) and piRFP (a gift from Michael Davidson & Vladislav Verkhusha ...
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bioRxiv - Molecular Biology 2024Quote: ... Lentivirus transduction vectors were derived from pLVX-puro (Clontech). For β-galactosidase transduction ...
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bioRxiv - Bioengineering 2024Quote: ... The IRES sequence was derived from pIRES2-EGFP (Clontech). Other sequences were derived from public databases ...
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bioRxiv - Cell Biology 2024Quote: ... The HEK293 T-derived cell line Lenti-X (Takara) was cultured in DMEM with 10% FBS and 1% PS ...
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bioRxiv - Cell Biology 2023Quote: The Ub(K48R)-IBM and Ub(K48R)ΔLRGG-IBM expression constructs were generated by gene synthesis and cloned to a Tet-on 3G inducible PiggyBac vector using In-Fusion HD enzyme (Clontech). The IgA2 and its IBM mutant constructs were cloned to a PCDNA3.1 vector under the control of a CMV promoter ...
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bioRxiv - Microbiology 2023Quote: Human kidney-derived Lenti-X 293T cells (TaKaRa, Cat# Z2180N) and porcine kidney-derived PK-15 cells (Japanese Collection of Research Bioresources Cell Bank ...
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bioRxiv - Cancer Biology 2022Quote: ... Sequences derived from pRetroX-G1-Red and pRetroX-SG2M (Clontech) were cloned into pHR lentiviral vectors under puromycin or blasticidin selection ...
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bioRxiv - Developmental Biology 2022Quote: ... Predicted enhancer elements were PCR-amplified from mouse genomic DNA (Clontech) and cloned into the respective LacZ expression vector (Osterwalder et al ...
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A gene desert required for regulatory control of pleiotropic Shox2 expression and embryonic survivalbioRxiv - Developmental Biology 2023Quote: ... Predicted enhancer elements were PCR-amplified from mouse genomic DNA (Clontech) and cloned into a Hsp68-LacZ expression vector for random integration114 ...
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bioRxiv - Genetics 2020Quote: ... that contained both of the transgenes was subcloned to a piggyBac vector using the In-Fusion® HD Cloning Kit from Takara with the primers pNE3_UAS_to_pBac.F ...
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bioRxiv - Synthetic Biology 2022Quote: ... Cas9GCN4 and the catalytic core of chromatinmodifying enzymes were cloned into PiggyBac recipient plasmids by homology arm recombination using In-fusion HD cloning (Takara #639650). Specifically ...
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bioRxiv - Neuroscience 2020Quote: ... Templates for assembly were derived from human whole-brain cDNA (Takara) for all cDNAs ...
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bioRxiv - Cancer Biology 2022Quote: ... Two RNA samples derived from normal brain were purchased from Clontech Laboratories and BioChain respectively.
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bioRxiv - Molecular Biology 2022Quote: ... These sequences were obtained from pPIGA3GFP (inverted repeats of piggyBac transposon [17]) and pAcGFP-Hyg C1 vector (CMVp-AcGFP and SV40p-Hyg) (632492; Takara Bio USA). Recombinant baculovirus was then produced using a Bac-to-Bac system ...
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bioRxiv - Molecular Biology 2021Quote: HEK293T derived Lenti-X™ cells (Takara Bio, Mountain View, CA, USA) and TZM-bl cells (ARP-8129 ...
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bioRxiv - Molecular Biology 2021Quote: ... we used a secreted Metridia luciferase reporter gene derived from pMetLuc2 (Clontech). The coding sequence of this gene was interrupted by the TP53 nonsense mutation R213X ...
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bioRxiv - Immunology 2023Quote: miRNA was extracted from myotube-derived EVs using TRIzol reagent (Takara Biotechnology, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... terminator sequences and miniTn7 elements were amplified using PrimerStar Max DNA Polymerase from Takara (templates indicated in supplemental table S3) ...
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bioRxiv - Molecular Biology 2023Quote: ... was cloned in a home-made inducible vector derived from pLVX-Tight-Puro (Clontech) to make it SIN by a deletion in the 3’ LTR ...
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bioRxiv - Physiology 2021Quote: ... PCR was performed with cDNA derived from 0.5 μg of total RNA with Extaq (TaKaRa). The primer sequences were 5’-CAACGCCCATCGTTTCATGG-3’ and 5’-CAAAAACTTGAAGCCCGCCA-3’ for NtGAox20 and 5’-ATGTGTTCGTTTCAGCCCGA-3’ and 5’-CCGCTGAAAAGTGTGCTTCC-3’ for tobacco arcA as an internal gene control (Ishida et al. ...
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bioRxiv - Microbiology 2020Quote: ... The PCR-amplified elements were ligated in a single reaction step by In-Fusion cloning (Clontech) upstream of the HA3 tag in XmaI and AfeI sites of pLN-HA3-loxP ...
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bioRxiv - Microbiology 2022Quote: ... Membrane was immunoblotted for ≥ 3 h with primary monoclonal anti-GFP (1:5,000) antibodies (JL8, Clontech-Takara), then followed by immunoblotting for ≤ 1 h with secondary antibodies ...
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bioRxiv - Microbiology 2022Quote: ... Membrane was immunoblotted for ≥ 3 h with primary monoclonal anti-GFP (1:5,000) antibodies (JL8, Clontech-Takara), then followed by immunoblotting for ≤ 1 h with secondary antibodies ...
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bioRxiv - Neuroscience 2020Quote: ... 341F (5’-CCTACGGGNGGCWGCAG-3’) and 805R (5’-GACTACHVGGGTATCTAATCC-3’) or (2) 341F (5’-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG-3’) and 806R (5’-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGGACTACHVGGGTWTCTAAT-3’) (TaKaRa Bio, Shiga, Japan). The second PCR was performed to add the index sequences for Illumina sequencing with barcode sequences ...
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bioRxiv - Cancer Biology 2023Quote: ... CT-2AZsGreen cells were generated using lentiviral particles derived from a pLVX-ZsGreen1C1 vector (Takara Bio #632566). All cell lines were grown in DMEM high glucose with 10% FBS (Gibco #16140-071 ...
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bioRxiv - Cancer Biology 2024Quote: ... DAXX mutant expression vectors were derived from the WT DAXX expression vector using In-Fusion mutagenesis (Takara) with primers as listed in Supplementary Table 1 ...
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bioRxiv - Neuroscience 2024Quote: ... a PGK promoter-driven mCherry expression cassette and the WPRE element were inserted into pMSCV (Clontech, Takara Bio) at the XhoI and ClaI sites to generate an empty vector (pMSCV-mCherry-WPRE) ...
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bioRxiv - Neuroscience 2024Quote: ... a PGK promoter-driven mCherry expression cassette and the WPRE element were inserted into pMSCV (Clontech, Takara Bio) at the XhoI and ClaI sites to generate an empty vector (pMSCV-mCherry-WPRE) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and glyceraldehyde 3-phosphate dehydrogenase (5′-GCACCGTCAAGGCTGAGAAC-3′ and 5′-TGGTGAAGACGCCAGTGGA-3′; Takara Bio Inc., Shiga, Japan) were used for RT-PCR.
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bioRxiv - Evolutionary Biology 2020Quote: ... 3 (Takara, Shiga), which is capable of resistance/nonspecific amplification and smear suppression against such PCR-inhibiting substances ...
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bioRxiv - Developmental Biology 2021Quote: ... was incubated with antibodies at 4°C for one hour: 3 µl (0.5 µg/µl) c-Myc monoclonal antibody (Cat. No. 631206, TaKaRa), or 1 µl (1.1 µg/µl ...
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bioRxiv - Neuroscience 2022Quote: ... pcDNA3.1-DCCR1343H-HA (c.4028G>A, p.R1343H) was derived from pcDNA3.1-DCCWT-HA using In-Fusion (Clontech 639648). Next ...
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bioRxiv - Neuroscience 2023Quote: ... comprising a red-shifted variant GFP cDNA and a downstream polyadenylation signal derived from pIRES2-EGFP (Takara Bio), was inserted in-frame into the initiation codon of the Scn1a coding exon 1 using the Red/ET Recombineering kit (Gene Bridges) ...
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bioRxiv - Genomics 2024Quote: ... the cDNA/DNA hybrids derived from the neuron series were amplified with PrimerSTAR GXL DNA polymerase (Takara Bio) for 10 cycles ...
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bioRxiv - Microbiology 2020Quote: ... or Oligo dT-3 sites Adaptor Primer for 3’ RACE (Takara). To analyze the complete BToV genome ...
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bioRxiv - Cell Biology 2020Quote: ... The emerin and LEM2 coding regions were amplified from HeLa cells derived cDNA and cloned into pEGFP-N3 (Clontech). pEGFP-N3-derived plasmids encoding for LAP2β (Ungricht et al. ...