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Citations for Takara Bio :
1 - 50 of 830 citations for Phosphatase Inhibitor Cocktail 2 Tubes 100X since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... 1X protease inhibitor cocktail (635673; Takara Clontech), 1 mM PMSF ...
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bioRxiv - Molecular Biology 2024Quote: ... 1X protease inhibitor cocktail (635673; Takara Clontech), 1 mM PMSF ...
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bioRxiv - Plant Biology 2023Quote: ... 0.05% T-20 and protease inhibitor cocktail (Takara)] ...
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bioRxiv - Neuroscience 2023Quote: ... and 10% glycerol with complete protease inhibitor cocktail (Clontech Laboratories). Cells were incubated on ice for 20 minutes before centrifugation at 14,000 rpm at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Lysozyme solution and Protease Inhibitor Cocktail (Takara Bio USA, Inc., CA). The suspension was sonicated 10 sec for 3 times with a 30 s pause ...
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bioRxiv - Molecular Biology 2024Quote: ... and 2 U recombinant inhibitor (TaKaRa) for samples containing biological inhibitor ...
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bioRxiv - Immunology 2023Quote: ... tubes with 1:1 FBS:PBS supplemented with recombinant RNase inhibitor (1:100, Takara). The live singlet gated CD3+ T cells were further gated as per the gating strategy shown in additional file 1 ...
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bioRxiv - Neuroscience 2019Quote: ... 2 U/μl RNase Inhibitor (Clontech/TaKaRa). We generated single cell RNA-Seq libraries using a modified Smart-seq2 method (Ding et al. ...
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bioRxiv - Neuroscience 2019Quote: ... 2 U/μl RNase Inhibitor (Clontech/TaKaRa). We generated single cell RNA-Seq libraries using a modified Smart-seq2 method (Ding et al. ...
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bioRxiv - Cell Biology 2021Quote: ... and pH7.2) supplemented with the ProteoGuard EDTA-free protease inhibitor cocktail (Clontech, 635673). The cells in the suspended solution were sonicated using the Q125 sonicator (Qsonica ...
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bioRxiv - Neuroscience 2022Quote: ... and 2 ul recombinant RNase inhibitor (Takara, 2313B). Isolated nuclei were sorted on a MA900 Multi-Application Cell Sorter (Sony Biotechnology) ...
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bioRxiv - Genomics 2024Quote: ... Supernatant containing viral particles was harvested after 2 and 3 days and concentrated 100x using Lenti-X concentrator (Takara) following manufacturers instructions.
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bioRxiv - Cell Biology 2019Quote: ... 0.5% CHAPS supplemented with protease inhibitor cocktail) were first applied to a His60 Ni Superflow column (Clontech). Eluates from the His60 Ni column were pooled and dialyzed overnight against 50 mM sodium phosphate pH 7.4 and then loaded onto a DEAE column ...
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bioRxiv - Microbiology 2022Quote: ... 2X ProteoGuard EDTA-free protease inhibitor cocktail (catalog no. 635673, Takara Bio USA, Inc., San Jose, CA, USA), and DNase and RNase-free RPI molecular grade water (catalog no ...
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bioRxiv - Immunology 2021Quote: ... 2.5 mM dNTP and 2 U/μL of recombinant RNase inhibitor (Clontech) then spun down and frozen at –80 °C.
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bioRxiv - Immunology 2021Quote: ... containing 2 μl lysis buffer per well (1:20 RNase inhibitor (Clontech) in 0.2% (v/v ...
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bioRxiv - Neuroscience 2022Quote: ... 2.5 mM dNTP and 2 U/mL of recombinant RNase inhibitor (Clontech) then spun down and frozen at −80°C ...
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bioRxiv - Neuroscience 2023Quote: ... 2.5 mM dNTP and 2 U/μL of recombinant RNase inhibitor (Clontech) then spun down and frozen at-80°C ...
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bioRxiv - Molecular Biology 2021Quote: ... and transferred to RT tubes (Maxima RT: Life Technologies, Inc., EP0753; 10mM dNTP Life Technologies, Inc., 4303443; RNAase inhibitor: Takara Bio ...
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bioRxiv - Neuroscience 2023Quote: ... we harvested 10-12 GFP+ cells per fly into 0.5ul nuclease free water in the pipette tip and then the tip was broken into a 96 well PCR tube containing RNAse inhibitors and buffer as described by Clontech’s ultra low HV SMARTer Ultra Low RNAseq kit (Catalog #634823) ...
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bioRxiv - Molecular Biology 2022Quote: ... the cell pellets were resuspended in the prepared cell lysis buffer (0.1% Triton-X, 20 mM Tris, 100 mM NaCl) and ProteoGuard EDTA-Free protease inhibitor cocktail (Takara) for 30 minutes followed by sonication in ice cold water for 40 minutes (75 amplitude ...
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bioRxiv - Immunology 2021Quote: ... 100,000 cells were sorted into 200 uL PBS with 1 uM DTT and 5 uL RNase Inhibitor Cocktail (Takara); for ex vivo culture experiments ...
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bioRxiv - Genomics 2022Quote: ... 16 µL 100x DAPI and 8 µL ICELL8 Second Diluent (Takara) were added and incubated 10 minutes at RT ...
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bioRxiv - Genetics 2022Quote: ... Produced lentiviruses were concentrated 100x with Lenti-X Concentrator (#631232, Takara) to increase virus concentration.
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bioRxiv - Molecular Biology 2022Quote: ... Produced lentiviruses were concentrated 100x with Lenti-X Concentrator (#631232, Takara) to increase virus concentration ...
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bioRxiv - Molecular Biology 2023Quote: ... Total protein was lysed by homogenization in radioimmunoprecipitation (RIPA) buffer (FUJIFILM) containing protease inhibitor cocktail (NACALAI TESQUE) and Cryonase Cold-active Nuclease (TAKARA), while total RNA was extracted using an RNeasy Mini Kit (Qiagen) ...
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bioRxiv - Cell Biology 2020Quote: ... The calf intestinal alkaline phosphatase (TaKaRa) was used as a standard for the reaction.
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bioRxiv - Immunology 2024Quote: ... Harvested supernatant was concentrated 100x using the Lenti-X Concentrator (Takara; 631231) and resuspended in complete DMEM and stored at -80°C ...
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bioRxiv - Developmental Biology 2021Quote: ... Single embryos were collected in 2 µl RNase free water with Recombinant RNase inhibitor (TAKARA) and ruptured with an RNase free needle ...
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bioRxiv - Cancer Biology 2020Quote: ... containing 2 μL of lysis buffer (0.2% Triton X-100, 4U of RNase inhibitor, Takara) per well ...
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bioRxiv - Bioengineering 2023Quote: ... Lentivirus was then concentrated 100X using the Lenti-X concentrator (Takara Bio,631232). Biological titration of lentivirus by QPCR was carried out as previously described86 ...
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bioRxiv - Cancer Biology 2021Quote: ... containing 2 μL of PBS including 0.2% Triton X-100 and 4U of RNase inhibitor (Takara) per well ...
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bioRxiv - Developmental Biology 2020Quote: ... Lentivirus was first concentrated 100x using Lenti-X Concentrator kit from Takara (Cat # 631231). Concentrated lentivirus was aliquoted and stored in −80 before use ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 μL of alkaline phosphatase (Takara, Okasa, Japan) was added ...
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bioRxiv - Genomics 2022Quote: ... Viral supernatant was collected at 48 hours and concentrated 100x using Lenti-X Concentrator (Clontech), resuspended in cell culture media ...
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bioRxiv - Cell Biology 2023Quote: ... according to the manufacturer’s instructions and concentrated ∼100x using the Lenti-X concentrator kit (Takara) and stored at -80°C until needed ...
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bioRxiv - Molecular Biology 2024Quote: ... Fresh tubes of RRI (Cat. 2313B TaKaRa) were used in all comparisons in this study.
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bioRxiv - Physiology 2021Quote: ... mixed with 5 μl lysis buffer (0.2% Triton X-100, with 2 U/μl recombinant RNase inhibitor, Clontech) at the end of the collection and were immediately snap-frozen on dry ice ...
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bioRxiv - Molecular Biology 2019Quote: ... an alkaline phosphatase (AP) tag (pSEAP; Clontech, CA, USA) was linked to the N-terminus of PAR1 cDNA (F2R ...
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bioRxiv - Synthetic Biology 2021Quote: ... and 0.5 U/ml Bacterial Alkaline phosphatase (Takara 2120A) at 37°C for 1 h in 10 µl of a reaction mixture containing 20 mM HEPES–KOH pH 7.5(Nacalai 15639-84) ...
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bioRxiv - Cell Biology 2022Quote: ... Lentiviral media was then kept at 4°C before 100x concentration using LentiX concentrator (Takara #631232) and resuspension of virus in organoid seeding media ...
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bioRxiv - Bioengineering 2023Quote: ... The viral supernatant was concentrated to 100x in 1x DPBS using Lenti-X Concentrator (Clontech, 631232) according to the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2021Quote: ... 10 μM ROCK inhibitor (Y-27632; Selleckchem, Cat. No. S1049) and 2 μg/mL doxycycline (Clontech, Cat. No. 631311). Media was changed daily during this stage.
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bioRxiv - Neuroscience 2024Quote: ... After the addition of 5 µl lysis buffer (0.2% Triton X-100, with 2 U/μl recombinant RNase inhibitor, Clontech) to the cap ...
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bioRxiv - Plant Biology 2019Quote: ... and dephosphorylated with Calf Intestinal Alkaline Phosphatase (Takara, cat#2250A). CRISPR targets were synthesized in oligonucleotide form and annealed to produce a double stranded fragment which was subsequently ligated to the BsaI treated vectors (Takara ...
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bioRxiv - Genomics 2019Quote: ... into individual wells of a 48-well plate (Brand) filled with 4 μl of a hypotonic lysis buffer containing 2 U/μL RNase inhibitor (Takara) and 0.2 % Triton-X-100 in Nuclease-free water ...
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bioRxiv - Cancer Biology 2019Quote: ... RNase inhibitor (Clontech), 2.5 mM dNTPs (Thermo Fisher ...
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bioRxiv - Immunology 2020Quote: ... RNAse Inhibitor (Takara), and ERCC RNA spike in controls (Ambion) ...
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bioRxiv - Immunology 2024Quote: ... RNAse Inhibitor (Takara)) in a 96-well V-bottom PCR plate (Thermo Fisher) ...
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bioRxiv - Immunology 2021Quote: ... using the 1.5mL centrifuge tube magnetic stand (Takara, #621964). The final library concentrations and qualities were determined by both SYBR PCR (Kapa Biosystems ...