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Citations for Takara Bio :
1 - 50 of 70 citations for Pemetrexed disodium Impurity 30 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2020Quote: ... A middle-copy (15-30 copies) episomal plasmid pGADT7 (Takara Bio) was used to increase the concentration of synthetic circuit components in order to buffer for the intrinsic molecular noise ...
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bioRxiv - Synthetic Biology 2023Quote: ... met14) was grown at 30 °C in YPDA medium (Clontech Takara). Yeast transformants were selected in Synthetic Defined (SD ...
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bioRxiv - Synthetic Biology 2023Quote: ... met14) was grown at 30 °C in YPDA medium (Clontech Takara). Yeast transformants were selected in Synthetic Defined (SD ...
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bioRxiv - Plant Biology 2024Quote: ... All probes were filtered on columns (CHROMA SPIN-30 columns, Clontech) to remove remaining nucleotides ...
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bioRxiv - Synthetic Biology 2022Quote: ... A middle-copy (∼10-30 copies) episomal plasmid pGADT7 (Takara Bio Inc.) was used to increase the concentration of proteins to buffer for the effects of intrinsic molecular noise and selected using different auxotrophic selection markers (Leucine ...
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bioRxiv - Microbiology 2023Quote: ... A middle-copy (~10-30 copies) episomal vector pGADT7 (Takara Bio Inc.) was used as the basic expression backbone for the introduction of chimeric proteins ...
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bioRxiv - Plant Biology 2023Quote: ... RNA from 30 nymphs was extracted using the RNAiso Plus kit (TaKaRa) according to the manufacturer’s instructions as one biological replicate ...
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bioRxiv - Plant Biology 2024Quote: ... and 14-30 nt ssRNA Ladder Marker (Takara™, catalog no. 3416). Gels were stained with 1X SYBR Gold Nucleic Acid Gel Stain (Invitrogen™ ...
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bioRxiv - Bioengineering 2023Quote: ... This was followed by a 30-minute DNase I (Takara Bio Inc., Japan) digestion at 37℃ ...
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bioRxiv - Immunology 2022Quote: ... coated with 30 μg/mL Retronectin reused up to three times (T100B, Takara), at 2000 x g for 2 hours at 32°C and replaced with activated human CD8+ T cells in fresh RPMI supplemented with 30 U/mL IL-2 ...
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bioRxiv - Systems Biology 2024Quote: ... 0.22μm filtered and concentrated 30 times by lenti-X concentrator (Takara Bio, 631231). The concentrated lentivirus was then aliquoted and stored at - 80°C until use.
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bioRxiv - Cell Biology 2024Quote: ... All PCRs were conducted for 30 cycles using Pyrobest DNA polymerase (Takara, R005A), PCR products were phosphorylated and ligated ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant (800 µL) was incubated with 30 µL of Talon beads (Takara Bio) at 4°C for 1 hour with continuous shaking ...
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bioRxiv - Neuroscience 2024Quote: ... and at 72°C for 30 sec in TaKaRa Thermal Cyclar Dice Touch (Takara).
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bioRxiv - Cell Biology 2023Quote: ... concentrated down 10 or 30 times using the Lenti-X Concentrator (631232; Takara Bio). Supernatants were kept at -80°C prior to being directly applied to target cells which were then spun at 700 xg for 1 h at 37°C ...
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bioRxiv - Genetics 2021Quote: ... The purified RNA (total 30 μg) was reverse transcribed using PrimeScript RT Master Mix (Takara). On-target or off-target sites of genomic DNA or cDNA were amplified with KOD Multi & Epi PCR kits (Toyobo ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA was extracted from pool of 30 embryos at 1 dpf using trizol reagent (Takara), chloroform (Sigma Cat ...
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bioRxiv - Molecular Biology 2020Quote: ... The membrane was dried and pre-hybridized at 37 °C for 30 min in ExpressHyb (Clontech). Hybridization was performed in ExpressHyb containing RI-labeled probe at 37 °C overnight with rotation ...
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bioRxiv - Genetics 2022Quote: ... Each 30 µL PCR reaction volume contained 15 µL Premix Ex Taq enzyme (Takara Biomedical Technology), 0.2 µL BSA ...
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bioRxiv - Neuroscience 2022Quote: ... Target sequences were amplified by 18–30 cycles of PCR using Ex-Taq™ (Takara Bio). For semiquantitative PCR ...
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bioRxiv - Systems Biology 2024Quote: Total RNA was extracted from 20-30 explants using a NucleoSpin RNA extraction kit (U955C, Takara). mRNA sequencing analysis was performed by BGI (Beijing ...
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bioRxiv - Bioengineering 2020Quote: ... Transformed strains were grown at 30 °C on agar plates containing minimal SD base (Clontech, cat. 630411) and –His/–Trp/–Ura dropout supplement (Clontech ...
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bioRxiv - Microbiology 2021Quote: ... Samples were incubated 30 min at RT and loaded onto a 10 μL aliquot of Talon (Takara) resin ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Ligation was performed by incubation at 16℃ for 30 minutes (DNA ligation kit Mighty mix, Takara, Japan) and the products were transformed into DH5α competent cells (Takara ...
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bioRxiv - Genetics 2022Quote: ... Each 30 µL PCR reaction volume contained 15 µL Premix Ex Taq enzyme (Takara Biomedical Technology, Beijing, China), 0.2 µL bovine serum albumin (BSA) ...
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bioRxiv - Evolutionary Biology 2020Quote: Colony PCR was performed with 16S-rRNA universal primers 8F (AGAGTTTGATCMTGGCTCAG) [28,29] and 1492R (GGYTACCTTGTTACGACTT) [30,31] in a 30 µL reaction using Ex Taq (TaKaRa). Amplification condition for the PCR included an initial denaturation step of 95 °C for 3 min ...
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bioRxiv - Microbiology 2023Quote: ... GISAID ID: EPI ISL 408667) [30] were generated by PCR using PrimeSTAR GXL DNA polymerase (TAKARA, Cat# R050A). A linker fragment comprising hepatitis delta virus ribozyme ...
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bioRxiv - Biophysics 2024Quote: ... Insoluble material was then removed by centrifugation at 50k x g for 30 min and Talon resin (Clontech) with 20 mM imidazole overnight binding at 4 °C ...
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bioRxiv - Developmental Biology 2020Quote: ... RNA (30 ng) was used for synthesis of cDNA with PrimerScript RT reagent kit with gDNA eraser (TAKARA BIO). QPCR was performed with TB Green Premix Ex-taq II (TAKARA BIO) ...
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bioRxiv - Bioengineering 2020Quote: ... The supernatant was collected by centrifugation at 25,000 g for 30 min and then incubated with Nickel-NTA agarose resin (Clontech) overnight at 4 °C ...
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bioRxiv - Biophysics 2021Quote: ... The supernatant was isolated by centrifugation at 160,000 g for 30 min and incubated with TALON superflow metal affinity resin (Clontech) at 4 °C overnight ...
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bioRxiv - Biophysics 2021Quote: ... The sample was clarified by centrifugation at 30,000 rpm for 30 min and the supernatant was then incubated with TALON resin (Clontech) supplemented with 10 mM imidazole for 3 h at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... The lysate was cleared by centrifugation (13000 g, 30 min) and the supernatant passed over a TALON resin (Clontech) and purified according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Retroviral supernatants were loaded by centrifugation (2,000xg for 2 hours at 30°C) onto non-tissue culture treated 6-well plates pre-coated with RetroNectin (20μg/mL; Takara). Activated T cells were added and spin-transduced for 30 minutes at 1,000xg ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was collected by centrifugation at 35,000 x g for 30 min and incubated with TALON IMAC resin (Clontech) and 20mM imidazole at 4 °C overnight.
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bioRxiv - Plant Biology 2023Quote: ... and SD4 (-Trp-Leu-His-Ade) media at 30℃ for 3–5 d according to the manufacturer’s manual (Clontech).
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bioRxiv - Cell Biology 2024Quote: ... after which virus-containing supernatants were harvested and concentrated 30–40 fold using a Lenti-X Concentrator (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Lysates were clarified by centrifugation at 100,000 × g for 30 min and incubated with His60 Ni Superflow affinity resin (Takara). Resin was washed with lysis buffer containing a stepwise gradient of 15-50 mM imidazole ...
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bioRxiv - Molecular Biology 2024Quote: ... Lysate was clarified by centrifugation at 100,000 × g for 30 min and incubated with His60 Ni Superflow affinity resin (Takara). Resin was washed with lysis buffer containing a stepwise gradient of 15–50 mM imidazole ...
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bioRxiv - Physiology 2021Quote: ... the pSTT91 and pGAD424 plasmids cloned with potential interactors were introduced into the Saccharomyces cerevisiae strain L40 and grown for 2 days at 30°C on a leucine- and tryptophan- deficient synthetic defined premixed yeast growth medium (SD-Leu-Trp, TaKaRa Clontech)(Hollenberg et al. ...
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bioRxiv - Genomics 2021Quote: ... Libraries from 4-cell embryos were also prepared using 18-30 nt gel purified RNA using the SMARTer smRNA-Seq Kit (Clontech) and NEXTflex-Small-RNA-Seq (New England Biolabs ...
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bioRxiv - Synthetic Biology 2023Quote: ... each for a 50-µL reaction containing ∼30 fmol of EcoRI-XbaI digested pLVSIN-CMV-Pur backbone plasmid (Takara #6183), ∼300 fmol of the insert fragment ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 μL of reaction was added to 50 μL Stellar Competent cells on ice for 30 mins followed by 45 second heat shock at 42 degrees C and resuspended in 500 μL SOC medium (Takara) and placed 1 hour shaking 200 rpm at 30 degrees C ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was obtained by centrifugation of the cell lysate at 10,000 g for 30 minutes and applied into the His TALON™ gravity column (Clontech). The columns were washed to remove the non-target proteins ...
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bioRxiv - Immunology 2024Quote: An adenovirus plasmid encoding the first 30 amino acids of KRAS with the G12V mutation (KRAS G12V-30) was created following the manufacturer’s instructions (Adeno-X CMV, Takara Bio). Adenovirus particles were produced by transfecting the adenoviral plasmid into HEK293 cells ...
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bioRxiv - Immunology 2022Quote: ... Lentiviral supernatants were spun onto nontreated wells plates coated with 30 μg/mL Retronectin reused up to three times (T100B, Takara), at 2000 x g for 2 hours at 32°C and replaced with activated human CD8+ T cells in fresh RPMI supplemented with 30 U/mL IL-2.
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bioRxiv - Plant Biology 2022Quote: ... Transferred membranes were blocked with 5% (w/v) milk for 30 mins at RT before being hybridized with the corresponding anti-GFP (632381, Takara) or anti-mCherry (632543 ...
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bioRxiv - Microbiology 2022Quote: ... The resultant lysate was cleared by centrifugation at 30 000 x g for 20 min and applied to Talon CellThru Co2+ chelating resin (TaKaRa-Clontech). The protein bound resin was washed with 3 column volumes (CV ...
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bioRxiv - Biochemistry 2023Quote: ... the insoluble material was removed by 50k x g centrifugation for 30 min and the supernatant was added to Talon resin (Clontech) with 20 mM imidazole to bind overnight at 4°C ...
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bioRxiv - Physiology 2021Quote: ... the pSTT91 and pGAD424 plasmids cloned with potential interactors were introduced into the Saccharomyces cerevisiae strain L40 and grown for 2 days at 30°C on a leucine- and tryptophan- deficient synthetic defined premixed yeast growth medium (SD-Leu-Trp, TaKaRa Clontech)(Hollenberg et al. ...