Labshake search
Citations for Takara Bio :
1 - 50 of 2138 citations for PI 3065 CAS 955977 50 1 98% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2021Quote: ... 50 ng/ml of doxycycline (Takara Bio USA, Mountain View, CA, USA) were added to each cell lines and cultured for 48 h.
-
bioRxiv - Biophysics 2020Quote: ... was cultured under the following conditions: cells were maintained in DMEM/F-12 50/50 medium containing 10% fetal bovine serum (tetracycline-free, Takara, Mountain View, CA), penicillin (100 units/ml) ...
-
bioRxiv - Microbiology 2022Quote: ... and 50 ng mL−1 or 25 ng mL−1 anhydrotetracycline (aTc, Clontech) and Aeromonas sp ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... from 12 ng of L001 library in a 50 μL reaction with PrimeSTAR (TAKARA, Mountain View, CA) using the following conditions ...
-
bioRxiv - Molecular Biology 2021Quote: ... and 1 µM of Shield-1 peptide (Clontech, Mountain View CA) for 4-6 hours ...
-
bioRxiv - Molecular Biology 2023Quote: ... and 1 μM of Shield-1 peptide (Clontech, Mountain View CA) for 4 h ...
-
bioRxiv - Neuroscience 2020Quote: ... containing 1% protease inhibitor (Clontech, Mountain View, CA) and 1% phosphatase inhibitors (Millipore Sigma) ...
-
bioRxiv - Molecular Biology 2022Quote: ... 6xHN Polyclonal Antibody (1:2000, Takara, CA, USA) at 4°C for overnight ...
-
bioRxiv - Cell Biology 2024Quote: hTERT Human Retinal Pigmented Epithelium-1 (hTERT-RPE-1, Clontech, CA, USA) cells were maintained in Dulbecco’s Modified Eagles Medium combined with high concentration of glucose ...
-
bioRxiv - Cancer Biology 2023Quote: ... lentiviral constructs pCMV-VSV-G and pCMV-dR8.2 dvpr 98 were transfected in HEK293T cells by the calcium-phosphate method (CalPhos Mammalian Transfection Kit, Takara, 631312) for virus production ...
-
bioRxiv - Neuroscience 2021Quote: ... Primary antibodies: anti-GFP (chick 1:20) and anti-DsRed (rabbit, 1:50, Takara Bio#632496). Secondary antibodies ...
-
bioRxiv - Neuroscience 2020Quote: ... STEM121 (human cytoplasm, 1:2000; Y40410, Takara, Mountain View, CA), overnight at room temperature to detect engrafted cells ...
-
bioRxiv - Microbiology 2023Quote: ... 5 U μL-1 Taq DNA polymerase (Takara, CA, USA), 1x of PCR buffer (10x ...
-
bioRxiv - Cell Biology 2024Quote: ... anti-mCherry 1:1000 (#632543 Takara Bio, San Jose, CA). Secondary antibodies donkey anti-Guinea pig Texas Red and goat anti-mouse FITC (Rockland Immunochemicals ...
-
bioRxiv - Biophysics 2024Quote: ... the supernatant was incubated with 1 ml 50% v/v Talon resin (Clontech) and 5 mM imidazole pH 8.0 per initial litre of cell culture for 1 h ...
-
bioRxiv - Immunology 2024Quote: ... the JR-CSF Env encoding fragments containing the desired alanine substitutions were ligated in-frame into a replication competent JR-CSF infectious molecular clone (pYK-JRCSF obtained through the NIH ARP, catalog no. 2708[98]) using InFusion technology (Takara Bio Inc., USA) according to instructions by the manufacturer ...
-
bioRxiv - Neuroscience 2020Quote: ... monoclonal mouse anti-STEM121 (1:500; Y40410, Takara, Mountain View, CA), polyclonal rabbit anti-GFAP (1:500 ...
-
bioRxiv - Cell Biology 2024Quote: ... GFP (Living Colors, Takara Biotech, San Jose CA, 632375; 1:2000), Pfn1 (Abcam ...
-
bioRxiv - Neuroscience 2022Quote: ... mouse anti-nc82 (1:50; Developmental Studies Hybridoma Bank) and rabbit anti-RFP (1:1000; TaKaRa Bio USA, #632496) at room temperature with agitation for 2 days ...
-
bioRxiv - Cancer Biology 2024Quote: ... 1 U/µL Recombinant RNase Inhibitor (Takara Bio, San Jose, CA, USA), 2,5 mM dNTPs (Thermo Fisher) ...
-
bioRxiv - Cancer Biology 2023Quote: ... 1-st PCR (PCR 1) was performed using Titanium Taq DNA Polymerase (# 639209, Takara Bio, CA, USA). Separation of the PCR products from primers and gel purification was done by QIAquick PCR & Gel Cleanup Kit (Qiagen ...
-
bioRxiv - Microbiology 2020Quote: ... Proteins were purified under denaturing conditions by metal affinity chromatography using a column packed with 50% His60 Ni2+ Superflow Resin (Clontech, Mountain View, CA, USA). The purified effector proteins were dialysed (Spectra/Por®3 Dialysis Membrane ...
-
bioRxiv - Immunology 2024Quote: ... one containing Mu Mx1 and one containing the Tol2 transposase were transfected into DF1 cells at 50-70% confluency using Xfect transfection reagent (Takara Bio, San Jose, CA) at a 5:1 ratio respectively ...
-
bioRxiv - Molecular Biology 2022Quote: ... was based on a pIRES2-EGFP vector (Clontech, Mountain View, CA; #6029-1). cDNA inserts from coding domain sequences (CDS ...
-
bioRxiv - Immunology 2020Quote: ... AP-1-Luc or NF-IL6-Luc plasmid (Clontech, Pal Alto, CA, USA) using Lipofectamine 2000 for 24 h (Stratagene ...
-
bioRxiv - Synthetic Biology 2024Quote: ... cultures were supplemented with 0.5 μg·ml−1 anhydrotetracycline (aTc) (Clontech, Mountain View, CA) and 1 mM neuraminic acid (Neu5Ac ...
-
bioRxiv - Synthetic Biology 2024Quote: ... cultures were induced with 0.5 μg·ml−1 anhydrotetracycline (aTc) (Clontech, Mountain View, CA). When experimental conditions required it ...
-
bioRxiv - Molecular Biology 2021Quote: ... were co-transfected into HEK293T cells at a 1:1:1:1.6:4.6 ratio using CalPhos mammalian transfection kit (TaKaRa Clontech, Mountain View, CA #631312) according to manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2021Quote: ... were co-transfected into HEK293T cells at a 1:1:1:1.6:4.6 ratio using CalPhos mammalian transfection kit (TaKaRa Clontech, Mountain View, CA #631312) according to manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2020Quote: ... The pHISi-1 vector was provided by the Matchmaker one-hybrid kit (Clontech, CA). The 40 bp promoter sequence was originally cloned from the maize C1 gene promoter (22) ...
-
bioRxiv - Biochemistry 2023Quote: ... and a fusion construct containing nucleotides of CPY (1-50) and Atg15ΔN35 was generated by ligation (Clontech). To generate pRS426-ATG15-3xFLAG (TPL003) ...
-
bioRxiv - Neuroscience 2021Quote: ... The coding region of rGAT-1 was inserted into pEYFP-C1 (Clontech, Palo Alto, CA). QuikChange Site-directed Mutagenesis kit was utilized to introduce the GAT-1 variants into a wildtype GAT-1 plasmid ...
-
bioRxiv - Biophysics 2022Quote: HIV-1 Gag (75) was ligated into pEGFP-N1 (Clontech, Takara Bio, Mountain View, CA) to generate the HIV-1 Gag-EGFP vector ...
-
bioRxiv - Biophysics 2022Quote: HIV-1 Gag (75) was ligated into pEGFP-N1 (Clontech, Takara Bio, Mountain View, CA) to generate the HIV-1 Gag-EGFP vector ...
-
bioRxiv - Neuroscience 2023Quote: ... Rabbit primary anti-RFP (anti-DsRed Living Colors, 632496, Takara, Mountain View, CA, 1:1000) was followed with donkey anti-rabbit AF594 (715-586-152 ...
-
bioRxiv - Cancer Biology 2024Quote: ... 50 ng/mL doxycycline (Clontech Laboratories) was added to the culture medium ...
-
bioRxiv - Genomics 2023Quote: ... The ligation reaction (1 µL) was transformed into 50 µL of Escherichia coli HST08 premium electro-cells (Takara, Japan) using a Gene Pulser Xcell (Bio-Rad ...
-
bioRxiv - Biochemistry 2022Quote: ... and mixed with 50 μL TALON Co2+ resin pre-equilibrated in binding buffer A (Takara Bio, 50% slurry) resulting in a final volume of 100 μL ...
-
bioRxiv - Molecular Biology 2020Quote: ... 0.4 μL ROX Reference Dye (50×) (TaKaRa), 0.8 μL of each primer (10 μM) ...
-
bioRxiv - Biophysics 2022Quote: ... YeastMaker DNA carrier 50 μg (Takara Bio), supplemented with 1 μg of pYeDP60 vector) ...
-
bioRxiv - Neuroscience 2023Quote: ... Tissues were rinsed four times for 15 min each with 0.1 M PBS and incubated with secondary antibodies anti-DsRed (1:500; RRID:AB_10013483; Living Colors DsRed polyclonal; Takara Bio USA, San Jose, CA) and anti-Troma1 (1:500 ...
-
bioRxiv - Microbiology 2021Quote: ... PCR amplifications were conducted in 50-μL total volumes containing 1 μL of template DNA using an ExTaq kit (Takara). The cycling protocol was as follows ...
-
bioRxiv - Molecular Biology 2022Quote: ... Lysate was cleared by centrifugation at 43 550×g and afterwards bound to 1 mL 50% slurry of Talon Metal Affinity Resin (TaKaRa) equilibrated in lysis buffer without PMSF ...
-
bioRxiv - Developmental Biology 2024Quote: ... 50 ng-1 ug of mRNA was reverse transcribed to cDNA using the PrimeScript RT Master Mix kit (Takara, RR036A). Real-time PCR was performed using the TB Green Premix Ex Taq kit (Takara ...
-
bioRxiv - Biochemistry 2020Quote: ... full-length (1-1143) or C-term (620-1143) was inserted into pGBKT7 (Clontech, Mountain View, CA) using SmaI/SalI restrictions sites ...
-
bioRxiv - Cell Biology 2022Quote: ... The extract was incubated for 1 h at 4 °C with Talon resin (Clontech, Mountain View, CA), loaded onto a column ...
-
bioRxiv - Neuroscience 2022Quote: ... brain sections were incubated with rabbit anti-DsRed antibody (1:1,000; #632496, Takara Bio., Mountain View, CA) at room temperature overnight ...
-
bioRxiv - Immunology 2022Quote: ... RNA (1 ng) was amplified using SMARTer library Ultra Low cDNA v4 kit (Takara Bio, Mountainview, CA). Sequencing cDNA libraries were preparing using a NexteraXT DNA library prep kit (Illumina ...
-
bioRxiv - Genetics 2024Quote: ... 1–1249 (full-length) were cloned into an EGFPC1 plasmid (N-terminal GFP tag) (Takara Bio, CA). For non-cleavable INF2 ...
-
bioRxiv - Cancer Biology 2023Quote: ... A bead ratio of 1x was used (50 µL of AMPure XP beads to 50 µL cDNA PCR product with 1 µL of 10x lysis buffer added, as per Clontech instructions), and purified cDNA was eluted in 17 µL elution buffer provided by Clontech ...