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Citations for Takara Bio :
301 - 350 of 2391 citations for PCR Tube since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... was amplified using CloneAmp HiFi PCR mix (Takara) in a 2-step PCR-program of 98°C for 10 seconds followed by 68°C for 4 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... or by CloneAmp HiFi PCR Premix (Takara Bio). Random mutagenesis was performed using Error-Prone PCR (EP-PCR ...
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bioRxiv - Genetics 2019Quote: ... PCR was performed using Taq polymerase (TAKR001C, ClonTech) when running DNA fragments on a gel ...
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bioRxiv - Cancer Biology 2020Quote: ... and SYBR-Green PCR Master Mix Kit (Takara) on an ABI7500 system (Applied Biosystem) ...
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bioRxiv - Molecular Biology 2020Quote: ... and Nucleospin Gel and PCR Clean-up (Takara). The sequencing library was sequenced with the Illumina Next-Seq 500 instrument (Illumina ...
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bioRxiv - Genetics 2021Quote: ... PCR was performed using PrimeSTAR HS (Takara Bio) and T100 Thermal Cycler (Bio-rad ...
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bioRxiv - Microbiology 2020Quote: ... The PCR products were digested with DpnI (TaKaRa) at 37°C for 1 h ...
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bioRxiv - Plant Biology 2021Quote: ... and In-Fusion Advantage PCR Cloning Kit (Clontech) were used ...
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bioRxiv - Genetics 2020Quote: ... PCR products amplified using LA Taq polymerase (Takara) or Multiplex PCR Kit (Qiagen ...
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bioRxiv - Genetics 2020Quote: ... Mutagenic PCR reactions used PrimeStar GXL polymerase (Takara), and 45mer mutagenesis primers (Table S2 ...
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bioRxiv - Cell Biology 2020Quote: ... EGFP was PCR-amplified from pEGFP-C2 (Clontech) and similarly cloned into the pRSET His-SS-TEV vector ...
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bioRxiv - Immunology 2020Quote: ... The riboswitch was amplified by PCR (CloneAmp, Takara; according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: Using PCR and In-Fusion cloning (Takara Bio), the ‘NGG’ PAM-recognition domain of the prime editor protein (PE2 ...
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bioRxiv - Microbiology 2022Quote: ... PCR was performed with Ex-Taq enzyme (TaKaRa) in a total volume of 30 µl containing 1 µl viral DNA ...
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bioRxiv - Genomics 2022Quote: ... PCR was performed using Taq polymerase (TAKR001C, ClonTech) when running DNA fragments on a gel ...
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bioRxiv - Biochemistry 2022Quote: ... PCR was performed using KOD DNA polymerase (Takara) with the following settings ...
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bioRxiv - Neuroscience 2022Quote: ... PCR amplification (PrimeSTAR Max polymerase, Takara Bio USA), and In-Fusion cloning (In-Fusion Snap Assembly Master Mix ...
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bioRxiv - Plant Biology 2022Quote: The In-Fusion Advantage PCR Cloning Kit (Clontech) and Gateway Cloning Technology (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... 1x of PCR buffer (10x, Takara, CA, USA), 10 μL of barcoded primer set forward/ reverse from ONT ...
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bioRxiv - Microbiology 2023Quote: ... In the In-Fusion PCR cloning system (Clontech), the S1 or S2 gene of the HV80 strain was replaced with the corresponding region of the H120 strain to construct the recombinant plasmids pMDVM8-HV80S1H120S2 and pMDVM8-H120S1HV80S2 ...
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bioRxiv - Developmental Biology 2023Quote: ... using CloneAmp™ HiFi PCR Premix (Takara 639298) and cloned it (in frame ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... PrimeScript RT-PCR Kit (Takara Bio, Shiga, Japan)
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bioRxiv - Cell Biology 2023Quote: ... using a Takara Thermal PCR Cycler (Takara Bio). RT-PCR of miRNAs was performed with a Taqman microRNA Reverse Transcription Kit (Invitrogen ...
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bioRxiv - Bioengineering 2023Quote: For PCR reactions PrimeSTAR HS DNA Polymerase (Clontech), Phusion High-Fidelity DNA polymerase (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... PCR was performed using Taq polymerase (TAKR001C, ClonTech) when running DNA fragments on a gel ...
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bioRxiv - Cell Biology 2023Quote: ... PCR amplification was conducted using PrimeSTAR Max (Takara) or KOD-Plus-Neo (TOYOBO).
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bioRxiv - Genetics 2023Quote: ... Then One-step PrimeScript RT-PCR kit (Takara) was used to generate cDNA ...
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bioRxiv - Plant Biology 2023Quote: ... the PCR products were digested with SifI (Takara) and cloned into the empty pCambia1305-3xFLAG or pCambia1305-3xHA vectors (gifted by Dr Yuli Ding ...
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bioRxiv - Genetics 2023Quote: ... and PCR amplified into 600 bp products (Takara). Indel frequencies were determined through Sanger data-decomposition using TIDE analysis tool ...
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bioRxiv - Immunology 2023Quote: ... Kit Advantage 2 PCR Enzyme System (Clontech, 639206), QIAquick Gel Extraction ...
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bioRxiv - Immunology 2024Quote: ... PCR was performed using Titanium Taq (Takara, #639208) with an extension time of 90s for 30 (1st round ...
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bioRxiv - Pathology 2024Quote: ... SYBR Green PCR reagent (Takara Co. Ltd., Japan) was chosen for real-time fluorescence quantification ...
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bioRxiv - Molecular Biology 2023Quote: ... All PCR analyses were performed using PrimeStarGXL (TaKaRa) as follows ...
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bioRxiv - Plant Biology 2020Quote: The polymerase chain reaction (PCR) was performed on genomic DNA using Emerald Amp MAX PCR Master Mix (Takara Bio, CA, USA) using the primers given in Table S2 ...
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bioRxiv - Developmental Biology 2021Quote: ... PCR donor amplicons were purified using the Nucleospin Gel and PCR Clean-up kit (Machery-Nagel, distributed by Takara Bio USA). PCR products were not gel purified ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and each RNA concentration was measured by quantitative PCR after reverse transcription using PrimeScript One Step RT-PCR Kit (TaKaRa, Japan) with specific primers (Table S2) ...
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bioRxiv - Molecular Biology 2021Quote: ... Gene expression in the cDNA samples was measured by one-step qRT-PCR using a One Step PrimeScript RT-PCR Kit (Perfect Real Time) according to the manufacturer’s specifications (Takara, Dalian, China). Quantitative real-time PCR was performed on the CFX Connect Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Bioengineering 2021Quote: ... To verify deletions or test for the presence of the CPOT plasmids colony PCR was performed using SapphireAmp fast PCR mix (TaKaRa Bio). For DNA construction ...
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bioRxiv - Bioengineering 2021Quote: ... Viral RNA from pseudovirus infected cells was also quantified by qRT-PCR using the Lenti-X qRT-PCR titration kit (Clontech; 631235) following manufacturer’s instruction ...
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bioRxiv - Microbiology 2022Quote: ... was used as the template for real-time RT-PCR performed according to the manufacturer’s protocol using the One Step TB Green PrimeScript PLUS RT-PCR kit (Takara, Cat# RR096A) and the following primers ...
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bioRxiv - Immunology 2022Quote: ... added with RT-PCR master mix and IgG VH/VL primers per well and performed with RT-PCR following the one step RT-PCR kit protocol (Takara, RR057A). Then ...
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bioRxiv - Microbiology 2020Quote: ... Quantitative real-time PCR was performed as described (Pujol et al., 2008b) by using SYBR™ Green PCR Master Mix (TaKaRa). Values were normalized to those of act-1 and were analyzed by the cycling threshold method using the appropriate qRT-PCR primers (Supplementary Table S2) ...
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bioRxiv - Microbiology 2021Quote: ... was used as the template for real-time RT-PCR performed according to the manufacturer’s protocol using the One Step TB Green PrimeScript PLUS RT-PCR kit (Takara, cat# RR096A) and the following primers ...
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bioRxiv - Genomics 2022Quote: ... the primers used in this PCR carry partial Illumina adaptor sequences that permit indexing with Illumina dual-indexing primers in the second step PCR: after cleaning up with NucleoSpin Gel and PCR Clean-Up (Takara Bio), amplified barcodes were indexed using Q5 hot-start DNA polymerase (New England Biolabs ...
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bioRxiv - Genetics 2022Quote: ... The primers are listed in the S8 Table and the PCR amplifications were performed using the CloneAmp™ HiFi PCR Premix (Takara). The pUAST-dCas9-KRAB plasmid was recombined into the attP site attP40 (BL #24749 ...
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bioRxiv - Genomics 2022Quote: ... Total RNA from SARS-CoV-2 infected lung or trachea was subjected to one-step real-time reverse transcription PCR using One-step PrimeScript RT-PCR kit (Takara, RR064B). Multiplex PCR was performed to detect SARS-CoV-2 nucleocapsid gene and mouse Actb gene ...
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bioRxiv - Cell Biology 2021Quote: ... Dbl3Y570D substitution was introduced into Dbl3-myc by PCR using the CloneAmp HiFi PCR premix and In-Fusion cloning (Clontech Inc.). Constructs were verified by sequencing ...
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bioRxiv - Cell Biology 2019Quote: ... This cDNA was used for real time PCR (RT-PCR) analysis using SYBR-Premix Ex Taq™ II (Tli RNaseH Plus) (Takara) with 10 pmol of specific primers ...
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bioRxiv - Genetics 2021Quote: Isolated RNA from OSF and control tissues was immediately subjected to realtime RT PCR (using Takara PrimescriptTM one step realtime RT PCR kit) (Takara, Japan) analysis for detection of changes in expression of NQO1 mRNA according to the manufacturers protocol ...
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bioRxiv - Microbiology 2021Quote: ... was used as the template for real-time RT-PCR performed according to the manufacturer’s protocol using the One Step TB Green PrimeScript PLUS RT-PCR kit (Takara, Cat# RR096A) and the following primers ...