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Citations for Takara Bio :
451 - 500 of 6188 citations for Osteopontin Human OPN ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... PCR products were cloned into vector pcDNA3.3 (Thermo Fischer Scientific) for human IgG1 expression using In-Fusion cloning technology (Clontech) as described previously[1] ...
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bioRxiv - Immunology 2021Quote: ... Antibodies were produced by transient transfection of human epithelium kidney 293T Lenti-X cells (Clontech Laboratories, Mountain View, CA) using the JetPrime transfection kit (Polyplus Transfection Illkirch ...
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bioRxiv - Molecular Biology 2021Quote: ... Human eRF1 coding sequence was cloned into the XhoI and HindIII sites of the pλN-HA-C1 vector (Clontech). The pλN-HA-C1-eRF1 plasmid was then used to generate the pλN-HA-C1-eRF1AAQ (G183A G184A ...
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bioRxiv - Cell Biology 2021Quote: ... US) and human cervical adenocarcinoma cells expressing a tetracycline-inducible repressor (HeLa Tet-Off, Clontech, Mountain View, California, US) were cultured in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Biophysics 2021Quote: The EGFR-GFP plasmid was constructed using the cDNA of human EGFR (pNeoSRαII) provided by Akihiko Yoshimura (Keio University) and was inserted into the pEGFP-C1 vector (Clontech) with the same linker sequence suggested by Carter and Sorkin (Carter and Sorkin ...
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bioRxiv - Cancer Biology 2023Quote: ... Individual lentiCRISPRv2 vectors were introduced along with packaging vectors into human embryonic kidney (HEK) 293T cells via calcium phosphate transfection according to the manufacturer’s protocol (Clontech). Lentivirus was harvested at 48 and 72 hours after transfection in RPMI media supplemented with 10% FBS and filtered with 45 μm filters prior to transduction of cancer cell lines using centrifugation at 1000 g for 2 hours in the presence of 8 μg/mL of polybrene (Santa Cruz Biotechnology) ...
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bioRxiv - Genomics 2023Quote: 200ng of RNA extracted from whole human blood used as input for reverse transcription using Smartscribe RT (Takara Clontech) with primers targeting the IGH constant regions (IDT ...
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bioRxiv - Genomics 2023Quote: 200ng of RNA extracted from whole human blood used as input for reverse transcription using Smartscribe RT (Takara Clontech) with primers targeting the IGH constant regions (IDT ...
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bioRxiv - Cell Biology 2023Quote: ... Gene expression profiles of mature hepatocytes were analyzed using human liver total RNA (636531, Clontech: Takara Bio, Shiga, Japan).
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bioRxiv - Cell Biology 2023Quote: ... Gene expression profiles of mature hepatocytes were analyzed using human liver total RNA (636531, Clontech: Takara Bio, Shiga, Japan).
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bioRxiv - Biochemistry 2024Quote: ... The sequence of human histone H2A(K15C-129) was cloned into the pCold-Trigger factor (TF) vector (Takara Bio) with a SUMO coding sequence inserted to generate His6–TF–SUMO–H2A(K15C-129 ...
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bioRxiv - Genetics 2021Quote: ... and were routinely cultured on gelatin-coated plates in t2i/L media: NDiff (N2B27) (Takara #y40002) supplemented with titrated 2i (0.2μM PD0325901 ...
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bioRxiv - Genetics 2020Quote: ... the plate was applied to a Thermal Cycler Dice® Real Time System II (TP900; TAKARA) with the following amplification conditions ...
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bioRxiv - Immunology 2019Quote: Non-tissue culture treated 12-well plates were coated overnight at 4C with 1mL Retronectin (Takara) at 25ug/mL in PBS ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were seeded in one well of a 6 well plate in supplemented DEF-CS (Takara). After a recovery period of 5 days ...
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bioRxiv - Plant Biology 2020Quote: ... and on a SD-Leu-Trp-Ade-His plate containing X-α-gal (Takara Bio, USA). Plates were imaged after incubation for 60 - 72 hr at 30°C unless otherwise stated ...
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bioRxiv - Synthetic Biology 2020Quote: ... Concentrated virus was plated on non-TC treated 6-well plates coated with retronectin (Takara T100B), and spun for 90 minutes at 1200xg ...
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bioRxiv - Immunology 2020Quote: ... the above plates were added with the RNase inhibitor and PrimeScript II Reverse Transcriptase (Takara, Japan) to a total volume of 10 μl ...
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bioRxiv - Neuroscience 2023Quote: Each lysis plate well contained 4uL of lysis buffer (4U Recombinant RNase Inhibitor (Takara Bio 2313B), 0.05% Triton X-100 ...
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bioRxiv - Immunology 2024Quote: ... The RNeasy kit and the cDNA Synthesis Kit (Takara, Cat#: 9767, RR047A) was used to extract total RNA and prepare cDNA following the manufacturer’s instructions ...
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Diversified expression of gamma-protocadherins regulates synaptic specificity in the mouse neocortexbioRxiv - Neuroscience 2021Quote: LATaq Kit (RR002B, TaKaRa) was used in nested PCR ...
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bioRxiv - Cancer Biology 2022Quote: ... 3’RACE kit (Takara) was used to convert RNAs of the prostate tumors into cDNAs by a reverse transcriptase and oligo-dT adapter primer ...
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bioRxiv - Genetics 2019Quote: First strand cDNA was synthesized from 1 μg total RNA by using a PrimeScript RT Reagent Kit with gDNA Eraser (Takara Bio, Kusatsu, Japan). qRT-PCR was performed in a 25 μl reaction volume with SYBR Premix Ex Taq II Tli RNaseH Plus (Takara Bio ...
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bioRxiv - Genomics 2020Quote: ... Full-length cDNA libraries were constructed from 1 μg of total RNA with SMARTer® cDNA synthesis kit (Takara Bio, Kusatsu, Shiga Japan), utilizing switching mechanism at 5’ end of RNA template (SMART ...
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bioRxiv - Plant Biology 2020Quote: ... First-strand cDNA was synthesized from 1 μg total RNA (20 μL reaction volume) using a PrimeScript™ 1st Strand cDNA Synthesis Kit (Takara, Dalian, China) according to the manufacturer’s protocol.
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bioRxiv - Neuroscience 2022Quote: ... Antennal cDNA was synthesized from 1 μg of antennal total RNA using SMARTer™ RACE cDNA amplification kit according to manufacturer’s instructions (Clontech, Mountain View, CA). To obtain full-length coding sequences ...
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bioRxiv - Physiology 2020Quote: ... containing 1 μL of 10× reaction buffer (SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing, Cat. No. 634888, Takara Bio, Kusatsu, Japan). Nuclease-free water was added to 11.5 μL ...
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bioRxiv - Cancer Biology 2019Quote: ... First-strand cDNA was synthesized from 1 μg of total RNA using the PrimeScript™ RT reagent Kit with gDNA Eraser (TaKaRa, Dalian, China). SYBR Premix Ex Taq II (TaKaRa ...
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bioRxiv - Genetics 2020Quote: ... RNA was extracted from samples using TRIzol and cDNA synthesized from 1 μg RNA template using PrimeScript™ RT reagent Kit with gDNA Eraser (TAKARA BIO INC). The sequences of primers used for amplification are shown in Table S6 ...
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bioRxiv - Cancer Biology 2021Quote: Total RNA (1 μg) was reverse transcribed to cDNA using the PrimeScriptTM RT reagent kit with gDNA Eraser (Cat#: RR047A, TaKaRa Bio, Shiga, Japan). qRT–PCR was performed using a LightCycler 480 II instrument (Roche Molecular Systems ...
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bioRxiv - Molecular Biology 2022Quote: ... Finally RNA was isolated from the eluates by phenol-chloroform-Isoamyl alcohol (25:24:1) pH 4.5 extraction and cDNA was prepared using 1st strand cDNA synthesis kit (Takara Bio Inc., Shiga, Japan) as described in above RNA analysis section ...
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bioRxiv - Plant Biology 2022Quote: ... Total RNA was isolated using Quick-RNA miniprep kit (ZymoResearch, CA, USA) and 1 μg of RNA was used to generate cDNA using SMART MMLV reverse transcriptase (Takara Bio USA, Inc.). Transcript abundance of an ABA biosynthesis gene ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: The cDNA was synthesized using 1 µg of RNA from each treatment using the PrimeScript™ RT reagent Kit with gDNA Eraser (TaKaRa Dalian, China). RT-qPCR was performed on a Bio-Rad CFX96 (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... Approximately 1 µg of total RNA was used for reverse transcription using the PrimeScript™ RT reagent kit with gDNA Eraser (Takara, Beijing, China). After the transcribed cDNAs were 5-fold diluted ...
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bioRxiv - Molecular Biology 2023Quote: ... the first-strand cDNA was synthesized from 1 μg total RNA using PrimeScript™ II 1st Strand cDNA Synthesis Kit (Takara, Beijing, China) following the manufacturer’s instructions.
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bioRxiv - Plant Biology 2023Quote: Genomic DNA was removed from 1 μg purified RNA using gDNA Eraser (PrimeScript RT Reagent Kit, Perfect Real Time) following manufacturer’s instructions (Takara Bio Inc., Kusatsu, Japan). Reverse transcription and cDNA was synthesized using PrimeScript Reverse Transcriptase (TaKaRa Bio) ...
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bioRxiv - Cell Biology 2020Quote: ... at the C-terminus were amplified by PCR using KOD-Plus-Neo polymerase (Toyobo) and Human Universal QUICK-Clone cDNA II (Clontech) for a template cDNA and then cloned into a pET-41 Ek/LIC vector (Novagen) ...
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bioRxiv - Developmental Biology 2021Quote: ... The guide RNA was designed to target an immediate downstream of the stop codon of the human GATA3 gene and generated using the Guide-it sgRNA In Vitro Transcription System (Clontech). The target sequences of gRNAs are shown in Table S2 ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA fragment of human METTL18 with a C-terminal HA sequence was cloned into AgeI and EcoRI sites of the pQCXIP vector (Clontech). To generate hMETTL18-Asp193Lys-Gly195Arg-Gly197Arg-HA ...
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bioRxiv - Biochemistry 2019Quote: ... 5’UTRs of MAGED2 TV2 and TV3 were amplified by RT-PCR from RNA isolated from human testes tissue purchased from Takara Bio (Mountain View ...
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bioRxiv - Plant Biology 2020Quote: ... Plasmids for the PARN activity assay were constructed by inserting the coding sequence of RRD1 or human PARN (hPARN) into the pHAT vector (Clontech). The hPARN sequence was derived from the GNP Human cDNA clone IRAK071M01 (RIKEN BioResource Research Center) ...
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bioRxiv - Cell Biology 2020Quote: ... 3C protease-cleavage site and a His12-tag at the N-terminus were amplified by PCR using KOD-Plus-Neo polymerase (Toyobo) and Human Universal QUICK-Clone cDNA II (Clontech) as a template cDNA and then cloned into a pET-41 Ek/LIC vector (Novagen) ...
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bioRxiv - Neuroscience 2020Quote: We transiently co-transfected cDNA constructs of GluN1 and GluN2A into mammalian human embryonic kidney 293 (HEK293) with a separate pEGFP-Cl vector (Clontech) at a ratio of 4.5:4.5:1 (GluN1/GluN2A/EGFP ...
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bioRxiv - Microbiology 2022Quote: Sequence of the human variable heavy and kappa chains were obtained by using SMARTer 5’ RACE technology (Takara Bio USA) adapted for antibodies to amplify the variable genes from heavy and kappa chains for each hybridoma ...
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bioRxiv - Cell Biology 2022Quote: pLVX-Puro GFP-TMEM11 was generated by PCR amplifying TMEM11 from human cDNA and cloning into the XhoI/BamHI sites of pAcGFP1-C1 (Takara), followed by subsequent cloning of the GFP-TMEM11 cassette into the Xho/BamHI sites of pLVX-Puro (Takara ...
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bioRxiv - Neuroscience 2021Quote: ... The cDNAs encoding full-length human HCN1 channel and mouse TRIP8b (splicing variant 1a-4) were cloned into the pcDNA 3.1 (Clontech Laboratories) mammalian expression vector ...
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bioRxiv - Molecular Biology 2022Quote: ... and Human GABRB3 (IMAGE ID 3871111, Source BioScience) were used to obtain N-terminal GST fusions in pGEX-KG (Clontech) or N-terminal FLAG fusions in pJEN1 (pcDNA3 derived ...
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bioRxiv - Neuroscience 2022Quote: The Yeast-Two-Hybrid screening for Jacob interaction partners was performed using a pretransformed human brain cDNA Library in pACT2 (Matchmaker-GAL4 Two-Hybrid II; Clontech) as described previously (Helmuth et al. ...
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bioRxiv - Immunology 2019Quote: ... Rab 5 and Rab 7 cDNA (a gift of M. Sandor) and human CD81 cDNA (Open Biosystems) were cloned into pmCherry-N1 vector (Clontech). Raji/DC-SIGN cells were electroporated with 1 µg of indicated plasmid using the Eppendorf Multiporator in iso-osmolar electroporation buffer using a 90 µs ...
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bioRxiv - Cell Biology 2019Quote: ... The coding sequences of these small GTPase proteins were amplified with polymerase chain reaction (PCR) using KOD-Plus-Neo DNA polymerase (Toyobo) and Human Universal QUICK-Clone cDNA II (Clontech) as template cDNA ...