Labshake search
Citations for Takara Bio :
1 - 50 of 152 citations for Octreotide acetate impurity H since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2020Quote: Ribonuclease H (RNase H) (20~60 U/μL) (Takara Bio Inc)
-
bioRxiv - Genomics 2023Quote: ... The final 65 μl volume was treated with 5 μl of an enzymatic mixture containing: 0.1 μl of RNase H (60 U/μl, Ribonuclease H
H>, catalog num. 2150, Takara), 2 μl of RNase ONE (10 U/μl ... -
bioRxiv - Plant Biology 2023Quote: ... and histidine (-H) (TaKaRa 630319).
-
bioRxiv - Cell Biology 2021Quote: ... 3 h and 0 h before extracting total RNA by MiniBEST Universal RNA Extraction Kit (Takara). The abundances of the interest genes were detected measured in each time point by real-time quantitative PCR (qPCR ...
-
bioRxiv - Developmental Biology 2021Quote: ... Lentiviral supernatant was harvested at 48 h and 54 h after transfection and concentrated using the lenti-X concentrator (Clontech) at 4°C for 12-36 h ...
-
bioRxiv - Genomics 2024Quote: ... RNA was digested with RNase H (Takara Bio) at 37°C for 30 min and the cDNA was purified with AMPureXP beads ...
-
bioRxiv - Neuroscience 2023Quote: ... The virus particles containing media were collected 24 h and 48 h after transfection and were concentrated using Lenti-X concentrator (Takara, 631232). The virus containing media was resuspended in Neurobasal media and tittered by puromycin selection using HEK 293T cell line ...
-
bioRxiv - Cell Biology 2022Quote: ... the relevant AD and BD constructs were co-transformed into the auxotrophic yeast strain AH109 using the polyethylene glycol/lithium acetate method according to the manufacture’s manual (Clontech). Yeast cells haboring the relevant combinations of constructs were dotted on plates with double (-Leu-Trp) ...
-
bioRxiv - Bioengineering 2023Quote: ... cerevisiae was carried out by using the lithium acetate method of Yeastmaker™ Yeast Tansformation System (CLONTECH Laboratories, Inc.). The plasmid of pICASA was cleaved by XbaI and integrated into the chromosomal DNA of S ...
-
bioRxiv - Molecular Biology 2022Quote: ... the spent medium was collected and filtered through a 0.45 μm cellulose acetate filter before concentrating the supernatant using Lenti-X concentrator (TaKaRa). Caco-2 cells were cultured at <50% confluence and transduced with lentiviral particles ...
-
bioRxiv - Plant Biology 2024Quote: ... the relevant combinations of constructs were co-transformed into the auxotrophic yeast strain AH109 using the polyethylene glycol/lithium acetate method according to the manufacturer’s manual (Clontech). Yeast cells were dotted on the plates of double (-Leu-Trp ...
-
bioRxiv - Microbiology 2020Quote: ... Transformation of AH109 cells with pGAD424- and pGBT9-based constructs by the lithium acetate method was performed following the guidelines in the Matchmaker two-hybrid system (Clontech). Double transformants were isolated on synthetic defined medium lacking leucine and tryptophan ...
-
bioRxiv - Neuroscience 2022Quote: ... spent medium was collected and filtered through a 0.45 μm cellulose acetate filter before concentrating the supernatant using Lenti-X™ concentrator (TaKaRa).
-
bioRxiv - Cell Biology 2020Quote: ... Cotransformation with pGBT9 and pGADT7 plasmids was performed by a modification of the lithium acetate procedure as described in the Yeast Protocols Handbook from Clontech. Plasmids expressing GAL4-activation domains and GAL4-DNA-binding domains contain the Leu2 and Trp1 selection markers ...
-
bioRxiv - Cell Biology 2022Quote: ... The yeast strain AH109 was transformed with the constructs in destination vectors using lithium acetate transformation (Yeast Protocols Handbook PT3024-1, Clontech). The transformants were grown on synthetic defined medium (0.67% yeast nitrogen base ...
-
bioRxiv - Molecular Biology 2024Quote: ... The AD and BD fusion vectors were introduced into yeast (Saccharomyces cerevisiae) strain Y2H Gold using the polyethylene glycol/lithium acetate method described in the Yeast Protocols Handbook (Clontech). Co-transformed colonies were initially grown separately on SD/-Trp/-Leu plates and further screened for growth on SD/-Trp/-Leu/-His/-Ade plates ...
-
bioRxiv - Cancer Biology 2024Quote: ... media containing the virus were harvested and filtered through a 0.22 mm pore cellulose acetate filter and then concentrated with the Lenti-X Concentrator (Takara Bio) before quantitative characterization with the Lenti-X p24 Rapid Titer Kit (Takara Bio) ...
-
bioRxiv - Immunology 2022Quote: The recombinant pGBKT7-PGRP3 bait construct was transformed into yeast strain Y2HGold via the lithium acetate method per manufacturer protocol (Takara Bio). Transformants were cultured on SD/-Trp (SDO ...
-
bioRxiv - Molecular Biology 2021Quote: ... Resulting cDNAs were treated with RNAse H (Cat#2150B; Takara Bio) and quantified by RT-qPCR using the PowerUp™ SYBR™ Green Master mix (Cat#A25742 ...
-
bioRxiv - Genetics 2021Quote: ... SYBR Premix Ex Taq II (Tli RNase H Plus) (Takara Bio), or Power SYBR Green PCR Master Mix (Applied Biosystems ...
-
bioRxiv - Plant Biology 2024Quote: ... TB Green Premix Ex Taq II (Tli RNase H Plus) (Takara) was used for qPCR analysis in an Mx3000P qPCR machine (Agilent) ...
-
bioRxiv - Biochemistry 2020Quote: ... was transformed with the appropriate plasmids (see figure legends) according to the manufacturer’s instructions using the lithium acetate procedure (Clontech Matchmaker 2 manual). Liquid cultures were grown overnight in standard dropout (SD ...
-
bioRxiv - Biochemistry 2023Quote: ... TB Green Premix Ex Taq II (Tli RNase H Plus) (Takara, #RR820A), RevertAid First Strand cDNA synthesis kit (Thermo scientific ...
-
Vitamin D signaling orchestrates skeletal muscle metabolic flexibility by regulating its fuel choicebioRxiv - Physiology 2023Quote: ... with SYBR® Premix Ex Taq (Tli RNase H Plus) by TAKARA. Each sample was amplified in triplicates using primers specific to genes of interest ...
-
bioRxiv - Molecular Biology 2024Quote: ... TB Green® Premix Ex Taq™ (Tli RNase H Plus; TAKARA) in a set volume of 10µL ...
-
bioRxiv - Plant Biology 2024Quote: ... and 1×SYBR Premix Ex Taq (Tli RNase H plus, RR420A; Takara). PCR conditions were as follows ...
-
bioRxiv - Developmental Biology 2021Quote: ... The supernatant was collected after 48 h and the Lenti-X concentrator (Clontech) was added to the suspension ...
-
bioRxiv - Cell Biology 2020Quote: ... After 24 h cells were transfected with an eGFP vector (3 μg; Clontech) using Fugene-6 HD reagent according to the manufacturer's protocol ...
-
bioRxiv - Genetics 2021Quote: ... or using SYBR Premix Ex Taq II (Tli RNase H Plus) (Takara Bio) on a LightCycler 480 II (Roche) ...
-
bioRxiv - Cell Biology 2024Quote: ... device using TB Green Premix Ex Taq II (Tli RNase H Plus, Takara) and the gene expression was normalized to Cyclophilin and presented ...
-
bioRxiv - Neuroscience 2023Quote: ... fixed for 1 h in 4% paraformaldehyde/PBS (TAKARA BIO INC. Cat#T900), and subjected to antibody labeling ...
-
bioRxiv - Developmental Biology 2024Quote: ... 5 µl of SYBR® Premix Ex Taq (Tli RNase H Plus) (Takara) and run in a CFX connect instrument (Bio-Rad) ...
-
bioRxiv - Developmental Biology 2024Quote: ... 5 µl of SYBR® Premix Ex Taq (Tli RNase H Plus) (Takara) and run in a CFX connect instrument (Bio-Rad) ...
-
bioRxiv - Genetics 2024Quote: ... We used TB Green Premix Ex Taq II (Tli RNase H Plus) (TAKARA) to perform quantitative real-time PCR on LightCycler LC480 (Roche ...
-
bioRxiv - Plant Biology 2020Quote: ... After 4 h total RNA was extracted using RNAiso Plus (Takara Bio, Shiga, Japan) according to the manufacturer’s protocol and used for real-time quantitative RT-PCR (qRT-PCR ...
-
bioRxiv - Cell Biology 2020Quote: ... the reverse transcription reactions were performed using reverse Transcriptase M-MLV (RNase H-) (TaKaRa). The cDNA was then used for real-time qPCR using the SYBR Green qPCR Master Mix (Bi-make ...
-
bioRxiv - Biochemistry 2022Quote: ... The dialyzed fraction was mixed for 3 h with Talon Metal Affinity Resin (Clontech) that had been equilibrated with Buffer T ...
-
bioRxiv - Microbiology 2020Quote: Lenti-X 293T™ cells (H. sapiens; sex: female) obtained from Takara (cat. #632180) and Huh-7.5 cells (H ...
-
bioRxiv - Cell Biology 2023Quote: ... cDNA was labelled with SYBR Pre-mix Ex Taq (Tli RNase H Plus, Takara) and the Cq values were obtained from the CFX96 TouchTM Real-Time PCR Detection System (BioRad) ...
-
bioRxiv - Developmental Biology 2024Quote: ... and 5 µl of SYBR® Premix-Ex-Taq (Tli RNase H Plus, Takara) in a 10 µl reaction ...
-
bioRxiv - Cell Biology 2021Quote: ... Lentiviral particles were collected 72 h after transfection and concentrated with Lenti-X Concentrator (Clontech) and stored at − 80°C ...
-
bioRxiv - Cell Biology 2020Quote: ... and TB Green™ Premix Ex Taq™ II (Tli RNase H Plus) (RR820L, Takara) with two repeats for each PCR ...
-
bioRxiv - Molecular Biology 2023Quote: ... and SYBR Premix Ex Taq II (Tli RNase H Plus) (Takara Bio INC, Kusatsu, Japan). All reactions were performed in at least three biological replicates ...
-
bioRxiv - Plant Biology 2024Quote: ... were diluted by a 10x series dilution and spotted onto –L/-T/-H medium (Clontech) to determine interactions between the two proteins.
-
bioRxiv - Microbiology 2023Quote: ... The cDNA was amplified using SYBR Premix Ex Taq II (Tli RNase H Plus; Takara) in a CFX96 Touch real-time PCR detection system (Bio-Rad ...
-
bioRxiv - Cell Biology 2020Quote: The GST-Bbs5 was expressed for 3 h at 27 °C in Escherichia coliBL21(DE3) (Takara) transformed with pGEX-4T-2-Bbs5-WT in the presence of 0.1 mM isopropyl-β-D-thiogalactopyranoside (Takara) ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... Membranes were baked for 2 h at 80°C and prehybridized in ExpressHyb (Clontech, Mountain View) at 65°C for 1h ...
-
bioRxiv - Physiology 2022Quote: ... PCR reaction was conducted using SYBR qPCR Premix Ex Taq II Tli RNase H+ (TAKRR820W, Takara). Primer pairs are listed in supplementary table S2.
-
bioRxiv - Cancer Biology 2020Quote: Cells were incubated with Ribonuclease H RNaseH (60 U/μl) (Takara Bio Inc., Code No. 2150A) for 4 hours before immunocytochemistry assay.
-
bioRxiv - Cell Biology 2023Quote: ... His-tagged recombinant proteins were isolated by 1 h incubation with Talon metal affinity resin (Clontech) at room temperature ...