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Citations for Takara Bio :
101 - 150 of 5773 citations for Mouse Two pore calcium channel protein 2 Tpcn2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: Yeast-2-hybrid assays were performed with the plasmids and strains from the Matchmaker Gold Yeast Two-Hybrid System (Takara Bio) according to manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... The virus titre was calculated using the ELISA-based Lenti-X™ p24 Rapid Titer Kit (Takara Bio) following the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2020Quote: Yeast two-hybrid experiments were carried out using the Matchmaker GAL4 Two-Hybrid System 3 (Takara Clontech) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: Yeast two-hybrid experiments were carried out using the Matchmaker GAL4 Two-Hybrid System 3 (Takara Clontech) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: The Yeast two-hybrid assay was performed using the Matchmaker™ Gold Yeast Two-Hybrid System (Clontech). The full-length coding region of ZmBZR1 were ligated into pGBKT7 plasmid as bait and the full-length coding regions of ZmBEH1 was fused into pGADT7 vector as prey ...
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bioRxiv - Cell Biology 2019Quote: ... Yeast two-hybrid assays were performed according to the Matchmaker Gold Yeast two-hybrid system manual (Clontech). Different combinations of constructs were co-transformed into yeast strain AH109 using the polyethylene glycol/lithium acetate method as described in the manual of Clontech ...
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bioRxiv - Plant Biology 2020Quote: Yeast two-hybrid analysis was employed using the MatchMaker GAL4 Two-Hybrid System 3 (Takara Bio, Japan) as previously described (Umezawa et al. ...
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bioRxiv - Microbiology 2021Quote: Yeast two-hybrid assays were performed in the yeast strain Y2Hgold with Matchmaker two-hybrid system (Clontech) according to manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2022Quote: The yeast two hybrid assay was performed as indicated in MATCHMAKER GAL4 two-hybrid system 3 (Clontech). The protein coding regions of genes used in this study were amplified from Guy11 cDNA with primer pairs listed in Table 1.1 ...
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bioRxiv - Biochemistry 2019Quote: ... Protein purity and concentration were determined by SDS-PAGE and BCA protein assay kit (Takara) respectively ...
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bioRxiv - Cell Biology 2022Quote: ... Total protein was quantified using the TAKARA BCA Protein Assay Kit (TAKARA Bio Inc., Japan). Equal amounts of protein were separated by SDS-PAGE on 10% gels ...
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bioRxiv - Neuroscience 2019Quote: ... RNA was divided in two equal samples and used for amplification with SMART RACE cDNA Amplification Kit (Clontech) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... The two fragments were assembled in a single In-Fusion reaction (In-Fusion HD Cloning Kit, Takara, 639650), and the PCR-derived regions of the resulting plasmids were confirmed by sequencing.
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bioRxiv - Cell Biology 2022Quote: Every protein expressed in mouse rods was also cloned into pEGFP-N1 (Clontech) for expression in AD293 cells using the AgeI and NotI cloning sites within the vector to replace EGFP with the tagged proteins ...
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bioRxiv - Plant Biology 2019Quote: Yeast two-hybrid assays were carried out using the Matchmaker™ gold Yeast two-Hybrid Systems (Clontech, USA). Different truncated CDS of EcaICE1 without transcriptional activation activity were subcloned into pGBKT7 (BD ...
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bioRxiv - Cell Biology 2020Quote: Yeast two-hybrid assays were performed according to the manual of Matchmaker Gold Yeast two-hybrid system (Clontech). The relevant combinations of constructs were co-transformed into yeast strain AH109 using the polyethylene glycol/lithium acetate method as described in the manual ...
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bioRxiv - Immunology 2023Quote: ... Kit Advantage 2 PCR Enzyme System (Clontech, 639206), QIAquick Gel Extraction ...
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bioRxiv - Biochemistry 2023Quote: ... The Bradford protein assay kit was purchased from Takara Bio (Shiga ...
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bioRxiv - Biophysics 2024Quote: Lentivirus titration was determined by p24 ELISA using Lenti-X™ p24 Rapid Titer Kit (Takara Bio, no. 632200) and a plate reader (PerkinElmer Victor3 V) ...
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bioRxiv - Molecular Biology 2020Quote: The Matchmaker Yeast Two-Hybrid System (Clontech) was used for protein–protein interaction analysis following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... the Matchmaker Yeast Two-Hybrid System (Clontech) was used according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: The Matchmaker Yeast two-hybrid system (Clontech) was used to test direct protein-protein interactions according to the manufacturer’s instructions ...
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The E3 ubiquitin-protein ligase MDM2 is a novel interactor of the von Hippel-Lindau tumor suppressorbioRxiv - Biochemistry 2020Quote: The Matchmaker Gold Two Hybrid System (Clontech) was used to detect direct interaction between MDM2 and pVHL (default conditions) ...
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bioRxiv - Plant Biology 2021Quote: The matchmaker GAL4 two-hybrid system (Clontech) was used for Y2H assays ...
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The Arabidopsis MCTP member QUIRKY regulates the formation of the STRUBBELIG receptor kinase complexbioRxiv - Plant Biology 2022Quote: The Matchmaker yeast two-hybrid system (Takara Bio Europe ...
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bioRxiv - Plant Biology 2023Quote: The Matchmaker LexA two-hybrid system (Clontech) was used for Y2H screening of an SBR-infected soybean cDNA library ...
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bioRxiv - Immunology 2021Quote: ... and the protein concentration was measured by BCA protein assay kit (TaKaRa, Dalian, China, cat#T9300A) as previous described(Ma et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein concentration was found to be 0.9 µg/µl using BCA Protein Assay Kit (TaKaRa).
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bioRxiv - Physiology 2022Quote: ... Obtained luminescence was normalized to total protein concentration measured by BCA protein assay kit (Takara Bio).
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bioRxiv - Microbiology 2024Quote: Protein concentrations were determined using the BCA method (TaKaRa BCA Protein Assay Kit; Takara, Shiga, Japan) after 1% SDS was added to solubilize the samples ...
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bioRxiv - Immunology 2021Quote: ... was used to for two-step qPCR assay after cDNA synthesis using PrimeScript® reverse transcriptase kit (Takara, Japan). Gene expression was normalized to the expression level of β-actin ...
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bioRxiv - Immunology 2023Quote: ... S-IgA was purified by two-step chromatography using the Capturem His-Taged Purification Kit (Takara Bio, Shiga, Japan) followed by size exclusion chromatography (Cytiva ...
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bioRxiv - Plant Biology 2023Quote: ... The Y2H assay was carried out based with the manufacturer’s protocol of Matchmaker Gold Yeast Two-Hybrid System Kit (Takara). The primers used for vector construction are listed in Supplemental Data Set S1.
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bioRxiv - Cell Biology 2022Quote: ... The yeast two-hybrid assays were performed according to the Matchmarker Gold Yeast two-hybrid system manual from Clontech. Different variations of the constructs were co-transformed by the polyethylene glycol/ lithium acetate method into the AH109 yeast strain and selected on SD/-Leu-Trp plates ...
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bioRxiv - Plant Biology 2022Quote: Yeast-two-hybrid library screening was performed according to the Matchmaker Gold Yeast Two-Hybrid System User Manual (Clontech).
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bioRxiv - Molecular Biology 2023Quote: The Yeast Two-Hybrid assays were performed according to the Matchmaker Gold Yeast Two-Hybrid System (Cat#630489; Clontech) kit instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... GFP fusion proteins were revealed with mouse monoclonal antibody anti-GFP (JL-8, Clontech) used at 1/2000 dilution ...
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bioRxiv - Molecular Biology 2022Quote: ... The 2× SYBR Green qPCR Mix Kit (TaKaRa, Japan) and the cDNA concentration and primers described above were used ...
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bioRxiv - Immunology 2022Quote: ... before amplification using the Advantage 2 PCR kit (Clontech) and the SINGV6 primer (95°C for 1 min ...
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bioRxiv - Bioengineering 2023Quote: ... quantified via qPCR (AAVpro Titration Kit version 2; Clontech), and stored at 4°C until use.
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bioRxiv - Evolutionary Biology 2024Quote: ... using the Advantage® 2 PCR Kit (Takara Bio) in a touchdown cycling program as follows ...
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bioRxiv - Microbiology 2020Quote: ... Protein concentrations of cell lysates were measured using a bicinchoninic acid protein assay kit (Takara Bio Inc.). Cell lysate containing the same amount of protein was mixed with 10 volumes of methanol and then centrifuged at 20,000 × g for 30 min to precipitate proteins ...
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bioRxiv - Plant Biology 2021Quote: Yeast two-hybrid assays were performed as described (Cesari et al., 2013) using the Matchmaker Gold Yeast Two-hybrid system (Clontech).
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bioRxiv - Plant Biology 2020Quote: The yeast two hybrid (YTH) experiments were performed in three replicates using the Matchmaker Gal4-based two-hybrid system (Clontech) and the Saccharomyces cerevisiae strain Y2HGold as described in (11) ...
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bioRxiv - Molecular Biology 2021Quote: ... as internal control by the calcium phosphate transfection method according to the manufacturer’s protocol (Takara). Raji cells (3×106cells ...
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bioRxiv - Bioengineering 2023Quote: ... 661W cells were transfected with the calcium phosphate technique or the XfectTM transfection reagent (Takara). MEF cells were transfected using TurboFectTM Transfection Reagent (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... and protein concentration was estimated by BCA Kit (Takara-#T9300A). For the assay ...
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bioRxiv - Genetics 2020Quote: Gal4-based Matchmaker Two-Hybrid System 3 (Clontech) was used according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... The two-hybrid system Matchmaker 3 from Clontech was used as per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... Two-hybrid plasmids were based on pGBT9 (Clontech) or the pGAD-C- and pGBD-C-series ...