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Citations for Takara Bio :
1 - 50 of 5174 citations for Mouse Meiotic Recombination Protein REC8 Homolog REC8 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Error-prone PCR of rec8+ cDNA in VP068-GFP-BsdR by Ex Taq DNA Polymerase (TaKaRa Bio) was performed using several concentrations of MnCl2 (final 0.05 mM ...
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bioRxiv - Microbiology 2023Quote: ... Genes were inserted by homologous recombination using an In-Fusion HD cloning kit (Clontech) into an in-house modified pETDuet plasmid to yield a cleavable N-terminal His-SUMO fusion protein.
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bioRxiv - Developmental Biology 2024Quote: ... and finally fused by a recombination reaction using In-Fusion HD Cloning Kit (Takara Bio). In-Fusion reaction solution was directly used for transformation of TOP10 strain of E ...
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bioRxiv - Neuroscience 2021Quote: ... and generated by In-Fusion recombination (Takara Bio). VGAT-SEP was constructed by fusing SEP to the luminal C-terminus of VGAT (NM_031782.2) ...
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bioRxiv - Biochemistry 2021Quote: pRM823 (pUC118-bamA) was constructed by in vitro recombination using In-Fusion HD cloning kit (Takara Bio) of a EcoRI-BamHI fragment from pUC118 and a bamA fragment prepared by PCR amplification from the genome of MC4100 using a pair of primers ...
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bioRxiv - Cell Biology 2020Quote: Lenti-X™ p24 Rapid Titration ELISA Kit (TaKaRa) was used to determine virus titer ...
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bioRxiv - Microbiology 2019Quote: Cloning via homologous recombination was carried out using the In-Fusion® HD cloning kit (Clontech Laboratories Inc). Primers used can be seen in Table 2.
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bioRxiv - Microbiology 2020Quote: ... ORF68 and its homologs were subcloned into the NotI and XhoI sites of pcDNA4/TO-2xStrep (N-terminal) using InFusion cloning (Clontech) (Addgene #x-x) ...
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bioRxiv - Plant Biology 2023Quote: ... We modified the recombination vector by using In-Fusion (Clontech) to insert the Gateway L1-L2 site into pJHY-TMp1 linearized by digestion with PacI ...
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bioRxiv - Cancer Biology 2020Quote: ... The titer of virus was measured using P24 ELISA kit (Clontech).The virus was aliquoted and stored at −80°C.
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bioRxiv - Molecular Biology 2020Quote: ... pnYK vector was created from pnYC vector (58) by homologous recombination between the chloramphenicol and kanamycin genes resistance gene using the In-Fusion kit (Clontech) following the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2021Quote: ... by subcloning it into the multiple cloning site of the pKT2/CAGXSP vector19 through recombination cloning (In-Fusion HD Cloning Kit, Clontech). For the EV reporter ...
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bioRxiv - Genetics 2021Quote: ... by homology arm recombination using In-fusion HD-Cloning (Takara #639650) according to manufacturer’s instructions (Fig ...
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bioRxiv - Cell Biology 2019Quote: ... Transformations using homology recombination were made into chemically competent Stellar (Clontech) E ...
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bioRxiv - Plant Biology 2023Quote: Plasmids for targeted gene deletion by homologous recombination were assembled using the In-Fusion® HD Cloning Kit (Takara Bio, Japan). Briefly ...
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bioRxiv - Biochemistry 2020Quote: ... viral titer was determined using the lentiviral p24 ELISA kit from Takara Bio (MountainView ...
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bioRxiv - Plant Biology 2019Quote: ... then assembled and amplified with attB recombination sites using PrimeSTAR polymerase (Clontech). The PCR product was purified by either QIAquick Gel Extraction Kit or QIAquick PCR Purification Kit (Qiagen) ...
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bioRxiv - Microbiology 2022Quote: ... All cloning were performed using In-Fusion recombination-based cloning method (Takara) and plasmids were introduced into M ...
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bioRxiv - Plant Biology 2023Quote: ... and transferred by LR recombination into pB42AD-GW and pGILDA-GW (Clontech), respectively ...
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bioRxiv - Cell Biology 2023Quote: TurboID plasmids were made using In-Fusion Recombination (Takara Bio USA, Inc.). 3xHA-TurboID was amplified from 3xHA-TurboID-NLS pcDNA3 (Addgene plasmid #107171 ...
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Deletion or inhibition of PTPRO mitigates diet-induced hepatic steatosis and inflammation in obesitybioRxiv - Immunology 2022Quote: ... Protein concentrations were assessed with the BCA protein assay kit (Takara BCA Protein Assay Kit, Takara, Shiga, Japan). SDS-PAGE and Western blotting were performed as previously described (Shintani et al. ...
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bioRxiv - Cell Biology 2022Quote: ... Lentiviral titers were determined using the LentiX-p24 Rapid Titer ELISA kit (Takara). All cell culture reagents were obtained from ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Concentrated viruses were titered using the p24-Elisa kit (Takara Bio, cat. 632200) following manufacturer’s protocols ...
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bioRxiv - Microbiology 2022Quote: ... This cassette was further modified by PCR and circularized by recombination (InFusion Takara) to replace gRNA1-tracerRNA1-gRNA2 by a NotI site ...
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bioRxiv - Bioengineering 2020Quote: ... and 21 were measured with an enzyme immunosorbent assay (ELISA) kit (Takara, Shiga, Japan).
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bioRxiv - Neuroscience 2020Quote: ... which were determined by an ELISA Adeno-X Rapid Titer Kit (Cat#: 631028, Takara). It detects the Adenoviral Hexon surface antigen ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentrations were measured using BCA Protein Assay Kit (TaKaRa). The samples were subjected to SDS-PAGE ...
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bioRxiv - Microbiology 2022Quote: ... The insertion was conducted by recombination of overlapping sequences using Infusion cloning (Clontech, USA), as previously reported (Park et al. ...
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bioRxiv - Zoology 2020Quote: ... The protein concentration was measured by TaKaRa BCA Protein Assay Kit (Takara Bio) in accordance with the manufacturer’s protocol ...
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Deletion or inhibition of PTPRO mitigates diet-induced hepatic steatosis and inflammation in obesitybioRxiv - Immunology 2022Quote: ... Protein concentrations were assessed with the BCA protein assay kit (Takara BCA Protein Assay Kit ...
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bioRxiv - Biochemistry 2023Quote: ... Protein concentrations were estimated using a BCA Protein Assay Kit (Takara). For FRAP experiments ...
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bioRxiv - Cancer Biology 2023Quote: ... The virus titer was determined with Lenti-X™ p24 Rapid Titration ELISA Kit (Takara), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... or mKate2 (Shcherbo et al., 2009) tags by homologous recombination via In-fusion Cloning (TaKaRa). 3 ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein concentration was measured using the BCA Protein Assay Kit (Takara Bio). The protein samples were subjected to SDS-polyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride membranes using the Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Microbiology 2022Quote: ... The protein concentration was determined by a BCA protein assay kit (TakaRa) using bovine serum albumin (BSA ...
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bioRxiv - Physiology 2022Quote: ... Protein concentration was determined using the BCA protein assay kit (Takara Bio).
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bioRxiv - Microbiology 2022Quote: ... Virus production was quantified by determining the amount of the Gag p24 protein using an enzyme-linked immunosorbent (ELISA) assay (Innogenetics or TaKaRa). For production of HIV-1 Env-pseudotyped viruses in Jurkat cells ...
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bioRxiv - Synthetic Biology 2020Quote: ... Protein concentrations were measured with a BCA protein assay kit (Takara, Dalian, China).
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bioRxiv - Microbiology 2023Quote: ... Protein concentration was quantified using the BCA protein assay kit (Takara Bio, T9300A). Cell lysates (about 200 µl ...
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bioRxiv - Microbiology 2020Quote: ... Viral titer was determined by the ELISA p24 antigen assay (Lenti-X p24 Rapid Titer Kit, TaKaRa). Monocytes were infected immediately after purification by adding HIV-1BaL virus to the culture at MOI between 3 and 5 based on the p24 titer ...
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bioRxiv - Microbiology 2022Quote: ... Viral titer was determined by the ELISA p24 antigen assay (Lenti-X p24 Rapid Titer Kit, TaKaRa). Infections of fully differentiated MDM were performed after adherent growth in the presence of M-CSF for seven days ...
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bioRxiv - Plant Biology 2021Quote: ... The fused proteins were purified with the GST-tagged protein purification kit (Clontech, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... The protein concentration was determined using the BCA protein assay kit (TaKaRa, Shiga, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... The protein concentration was quantified using the TaKaRa BCA Protein Assay Kit (TaKaRa, Japan), and 2-mercaptoethanol (Nacalai Tesque ...
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bioRxiv - Bioengineering 2023Quote: ... Using a BCA protein kit (TaKaRa, Shiga, Japan), a working solution (BCA reagent A ...
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bioRxiv - Immunology 2021Quote: ... Following bulk BCR and TCR are prepared using SMARTer Mouse BCR IgG H/K/L Profiling Kit and SMARTer Mouse TCR a/b profiling kit separately (Takara). Based on the extracted mRNA amount of each sample ...
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bioRxiv - Bioengineering 2021Quote: ... The virus titre was calculated using the ELISA-based Lenti-X™ p24 Rapid Titer Kit (Takara Bio) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... and the 3’- M6 fragment and the sfGFP fragment were inserted using In-fusion cloning (homologous recombination; TaKaRa). The resulting M6-sfGFP insert was excised using NotI and KpnI and ligated into pUASt-attB [38] ...
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bioRxiv - Developmental Biology 2021Quote: ... a single-stranded donor for homologous recombination was generated using the Guide-it Long ssDNA Production System (Clontech). The gRNA ...
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bioRxiv - Developmental Biology 2022Quote: ... PCR-amplified DNA fragments were assembled and cloned into pET-in situ Ble plasmid by recombination (InFusion, Clontech) and the resulting cell line was named ABbin situ ...