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Citations for Takara Bio :
1 - 50 of 4979 citations for Mouse Immunoglobulin G IgG CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Following bulk BCR and TCR are prepared using SMARTer Mouse BCR IgG H/K/L Profiling Kit and SMARTer Mouse TCR a/b profiling kit separately (Takara). Based on the extracted mRNA amount of each sample ...
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bioRxiv - Immunology 2023Quote: ... and used to generate Illumina-ready heavy and light chain sequencing libraries using the SMARTer Mouse BCR IgG H/K/L Profiling Kit (Takara, Cat# 634422). Briefly ...
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bioRxiv - Immunology 2022Quote: ... RT-PCR products were nested PCR-amplified with primers for IgG VH or IgG VL following the HS DNA polymerase kit protocol (Takara, TAK R010). The IgG VH and IgG VL nested PCR products were purified and next cloned into human IgG1 heavy chain and light chain expression vectors ...
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bioRxiv - Immunology 2022Quote: Matched healthy donor RNA was used to generate targeted IgG and IgM AIRR-seq libraries using the SMARTer Human BCR IgG IgM H/K/L Profiling Kit (Takara Bio USA) according to the manufacturer’s instructions with no modifications ...
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bioRxiv - Immunology 2024Quote: ... and pMD2-VSV-G to HEK293T cells using the CalPhos Mammalian Transfection Kit (Clontech Laboratories). Viral supernatants were filtered through 0.45 μm low-protein-binding filters ...
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bioRxiv - Molecular Biology 2021Quote: ... Both types of DNA fragment (0.1 µg) were used as templates to synthesize RNA using an in vitro transcription kit (in vitro Transcription T7 Kit; Takara Bio, Shiga, Japan) in 1.5 mL centrifuge tubes ...
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bioRxiv - Immunology 2022Quote: ... The SMARTer Mouse TCR a/b Profiling Kit (Takara, Cat. No. 634403) was used to amplify both TCRα and TCRβ sequences ...
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bioRxiv - Cell Biology 2020Quote: ... and pVSV-G (Takara Bio), followed by viral amplification in cells for 72 hours and addition of viral particles to HeLa cells ...
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bioRxiv - Cell Biology 2022Quote: ... pVSV-G (PT3343-5, Clontech) and psPAX2 (Addgene plasmid #12260 ...
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bioRxiv - Cell Biology 2024Quote: ... pVSV-G (PT3343-5, Clontech) and psPAX2 (Addgene plasmid #12260 ...
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bioRxiv - Immunology 2019Quote: ... The cDNA was generated and amplified by SMARTer Mouse TCRαβ Profiling Kit (Clontech). Libraries were sequenced by illumina sequencer MiSeq (2×250 cycles) ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 g RNA was used to synthesize the cDNA using reverse transcription M-MLV (RNase-free) kits (TaKaRa, Japan). qRT-PCR was performed with SYBR green PCR master mix (ToYoBo ...
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bioRxiv - Developmental Biology 2023Quote: ... and the viral packing vectors (pCMV-ΔR8.9 and pCMV-VSV-G) using the CalPhos mammalian transfection kit (631312, Clontech). pCW57.1-Luciferase and pCW57.1-mDux-CA were a gift from Stephen Tapscott (Addgene plasmids #99283 and #99284) ...
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bioRxiv - Genomics 2022Quote: ... IgG and IgM 5’RACE AIRR-seq libraries were generated using the SMARTer Human BCR Profiling Kit (Takara Bio), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... were transfected with pVSV-G (Clontech) and a pBABE vector containing both a gene for YFP-tagged histone H2B protein and for a gene encoding G418 resistance ...
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bioRxiv - Cancer Biology 2020Quote: ... 2.2 μg pCMV5-VSV-G (Clontech) were diluted in 600 μl OptiMem (Gibco ...
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bioRxiv - Immunology 2020Quote: ... and 160 ng pDM2.G (Clontech), 1.3 μg CMV 8.91 (Clontech) ...
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bioRxiv - Bioengineering 2023Quote: ... 2 µg pantropic pVSV-G (Clontech), 3 µg pCL- (Imgenex) ...
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bioRxiv - Cell Biology 2023Quote: ... and pVSV-G (#PT3343-5, Clontech) vectors were transfected using Lipofectamine 3000 ...
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bioRxiv - Genomics 2020Quote: ... 0.8μg of Total RNA was utilized as template for RT with random hexamer primers using PrimeScript RT reagent Kit (Takara). qRT-PCR was performed with respective gene-specific primers (PD GFD ...
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bioRxiv - Cell Biology 2023Quote: ... the transfer plasmid (pBABEpuro-based or pLZRS-IRES-ΔNGFR/GFP-based) and pCMV-VSV-G were co-transfected into GP2-293 packaging cells using CalPhos mammalian transfection kit (Takara/Clontech) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: Mouse tail DNA was amplified using KAPA Taq PCR Kit (Takara Bio, cat. R040A) and primers Rosa26 WT F ...
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bioRxiv - Cancer Biology 2020Quote: ... 1 µg of total RNA served as a template for cDNA synthesis with a PrimeScript™ 1st strand cDNA Synthesis Kit (Takara). GAPDH and 18S ribosomal RNA were amplified as reference genes for target mRNA ...
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bioRxiv - Cell Biology 2021Quote: ... lentiviral shRNA expression constructs were transfected together with packaging vectors (VSV-G, RSV-Rev and GAG-Pol) into 293T producer cells using CalPhos Transfection Kits (Takara Bio) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... 1 μg of total RNA from each condition was converted into cDNA using PrimeScript RT reagent with gDNA Eraser kit (Takara Clontech) and MJ Research PTC-220 Dyad PCR System (Conquer Scientific) ...
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bioRxiv - Immunology 2022Quote: ... The g block and linearized plasmid backbone were ligated together using the In-fusion HD cloning kit (Takara Bio, catalog #638920).
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bioRxiv - Microbiology 2021Quote: ... 1 μg of RNA was used for library preparation with the SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian (Takara Bio), following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... Each sample (2 μg) of total RNA was used to synthesize first-strand cDNA with a PrimeScript RT Kit with gDNA eraser (Takara, RR0447A). RT-qPCR was performed using a Roche LightCycler 480 system with a SYBR Fast qPCR Kit (KAPA Biosystems ...
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bioRxiv - Neuroscience 2023Quote: ... We then synthesized these sequences as G-blocks (Integrated DNA Technologies) and cloned them using Infusion kit (Takara biosciences catalog # 638948) into pSMART-HC-Kan (Lucigen ...
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bioRxiv - Cell Biology 2023Quote: ... the transfer plasmid (pBABEpuro-based or pLZRS-IRES-ΔNGFR/GFP-based) and pCMV-VSV-G were co-transfected into GP2-293 packaging cells using CalPhos mammalian transfection kit (Takara/Clontech) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... pVSV-G (631530, Clontech, Mountain View, CA) and psPAX2 ...
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bioRxiv - Immunology 2022Quote: ... added with RT-PCR master mix and IgG VH/VL primers per well and performed with RT-PCR following the one step RT-PCR kit protocol (Takara, RR057A). Then ...
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bioRxiv - Cell Biology 2023Quote: ... Retroviruses with the vesicular stomatitis virus–G (VSV-G) envelope were produced by transfection of GP2-293 cells (Clontech) with the pRetroX construct and pVSV-G (Clontech ...
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bioRxiv - Genomics 2021Quote: ... Purified RNA samples were diluted to 1 μg/μL and pooled to perform cDNA synthesis by utilizing PrimerScript first strand cDNA synthesis kit (Cat. no. 6110A, Takara Bio, Japan), following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... and TCR beta chain libraries were generated using SMARTer Mouse TCR a/b Profiling Kit (Clontech). Samples were pooled to a final pool concentration of 4 nM and diluted to a final concentration of 13.5 pM ...
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bioRxiv - Immunology 2022Quote: ... with pQCXIP constructs and pVSV-G (Clontech Laboratories). Jurkat NFAT luciferase (JNL ...
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bioRxiv - Cell Biology 2023Quote: ... with the pRetroX construct and pVSV-G (Clontech) using Lipofectamine LTX reagent (Invitrogen) ...
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bioRxiv - Molecular Biology 2021Quote: ... One µg of total RNAs was subjected to reverse transcription into cDNAs using PrimeScriptTM RT reagent Kit with gDNA Eraser (Takara, cat. no. RR047A) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... the lentiviral transfer plasmid pCSII-EF-luciferase (Agarwal et al., 2006), and pCMV-VSV-G (Stewart et al., 2003) using the CalPhos Mammalian Transfection Kit (Takara Bio Company, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... Add 25 mL 200 g/L glucose and 25 mL 20 g/L amino acid drop-out mix (Clontech. Inc., Japan) solution to prepare the medium] ...
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bioRxiv - Immunology 2022Quote: ... Bulk TCR sequencing was performed using the SMARTer® Mouse TCR a/b Profiling Kit (Takara Bio). Libraries were sequenced using the 600-cycle MiSeq Reagent Kit v3 (Illumina ...
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bioRxiv - Immunology 2022Quote: ... IGK and IGL 5’RACE AIRR-seq libraries were generated using the SMARTer Mouse BCR Profiling Kit (Takara Bio ...
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bioRxiv - Biophysics 2024Quote: VSV-G VLPs containing Vpr-eGFP and Vpr-StayGold were produced using Lenti-X™ Packaging Single Shots (VSV-G, Takara Bio, no. 631275), with pLVXS-ZsGreen1-Puro (Takara Bio ...
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bioRxiv - Cancer Biology 2021Quote: ... using Takara’s Lenti-X (VSV-G) Packing Single Shots (Takara Bio/ Clontech Laboratories ...
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bioRxiv - Neuroscience 2021Quote: ... MYC (mouse, Clontech, 631206 ...
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bioRxiv - Microbiology 2021Quote: ... 5′-RACE cDNA was obtained from bulk-sorted splenic B cells of each mouse with the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (TaKaRa). The IgG PCRs were set up with Platinum Taq High-Fidelity DNA Polymerase (Life Technologies ...
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bioRxiv - Immunology 2021Quote: ... 5’ s-RACE cDNA was obtained from bulk-sorted B cells of each mouse with the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (TaKaRa). The IgG PCRs were set up with Platinum Taq High-Fidelity DNA Polymerase (Life Technologies ...
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bioRxiv - Neuroscience 2022Quote: Total RNA from mouse cochleae was extracted using an RNA Extraction Kit according to the manufacturer’s instructions (#9767, TaKaRa, Japan). The extracted RNA concentration was measured using a Nanodrop 2000 spectrophotometer (#ND-LITE ...
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bioRxiv - Microbiology 2019Quote: ... 5’-RACE cDNA was obtained from bulk-sorted splenic B cells of each mouse with SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (TaKaRa). The immunoglobulin PCRs were set up with Platinum Taq High-Fidelity DNA Polymerase (Life Technologies ...
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bioRxiv - Neuroscience 2021Quote: Total RNAs from the mouse forebrain were reverse transcribed using the PrimeScript II first strand cDNA Synthesis Kit (Takara Bio) with the random hexamer primer ...