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Citations for Takara Bio :
251 - 300 of 5532 citations for Mouse Cytochrome C Oxidase Subunit II COX2 MT CO2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... and TCR beta chain libraries were generated using SMARTer Mouse TCR a/b Profiling Kit (Clontech). Samples were pooled to a final pool concentration of 4 nM and diluted to a final concentration of 13.5 pM ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA was synthesized at 44°C for 15 min using the PrimeScript RT reagent kit with gDNA eraser (TaKaRa) and a y300 PAT universal C10 primer ...
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bioRxiv - Cell Biology 2023Quote: ... 2xmCh-KIF5C in pcDNA3.1 was cloned by inserting in-frame KIF5C full length from EGFP-KIF5C (a gift from Anthony Brown) 2xmCh sequence from KIF5C(1-560)-2xmCh-EF(C) into pcDNA3.1 using InFusion Cloning kit (Takara). EGFP-Rab7A (Addgene #28047 ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was synthesized at 44°C for 15 min using the PrimeScript RT reagent kit with gDNA eraser (TaKaRa) and the y300 PAT universal C10 primer ...
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bioRxiv - Microbiology 2021Quote: ... the cDNAs were reverse-transcribed from 5 µg of total RNA with the PrimeScript™ II 1st Strand cDNA Synthesis Kit (TaKaRa, Dalian, China). Real time PCR (qPCR ...
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bioRxiv - Cell Biology 2022Quote: ... and Foxa3 were obtained by reverse transcription PCR using a template RNA extracted from a C57BL/6J liver using a PrimeScript™ II 1st strand cDNA Synthesis Kit (TAKARA Bio, Japan). The coding sequences of the primers used are listed in Table S3 ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was synthesized from total RNA of each culture sample using the PrimeScript II 1st strand cDNA Synthesis Kit (Takara Bio, Shiga, Japan) with random hexamers to yield a total volume of 20 μL ...
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bioRxiv - Immunology 2022Quote: RNA was extracted from LNs and used for RT-qPCR using a Prime Script™ II Strand cDNA Synthesis kit (Takara, Dalian, China), in accordance with the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... the first-strand cDNA was synthesized from 1 μg total RNA using PrimeScript™ II 1st Strand cDNA Synthesis Kit (Takara, Beijing, China) following the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2019Quote: ... Cells were maintained at 37 °C in a humidified atmosphere at 5% CO2 in DMEM 4.5g/L Glucose with UltraGlutamine media supplemented with 10% of Tet-free FBS (Clontech) and 1% penicillin/streptomycin.
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bioRxiv - Immunology 2022Quote: ... Bulk TCR sequencing was performed using the SMARTer® Mouse TCR a/b Profiling Kit (Takara Bio). Libraries were sequenced using the 600-cycle MiSeq Reagent Kit v3 (Illumina ...
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bioRxiv - Developmental Biology 2019Quote: ... plasmid after digestion of the inserts by EcoRI and KpnI and cloning into the EcoRI and EcoRV sites in frame with the C-terminal Flag tag in pSNAPf plasmid using In-Fusion Kit (Clontech). The human HSF2-YFP was constructed by PCR and cloned into the XhoI and SalI sites in frame with the N-terminal YFP tag in EGFp-C1 plasmid using In-Fusion Kit (Clontech) ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... and a modified vector with an N-terminal AviTag for biotinylation and C-terminal His6-tag (p28BIOH-LIC) using a ligation-independent InFusion cloning kit (ClonTech) and verified by DNA sequencing ...
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bioRxiv - Microbiology 2019Quote: ... These PCR products were cloned into pCA24N with a C-terminal GFP tag using the In-Fusion HD enzyme kit (Takara). Clones were selected on TSA plates with 50µg/ml chloramphenicol ...
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bioRxiv - Molecular Biology 2019Quote: ... The RNA was subjected to reverse transcription (RT) for 15 min at 37°C with the use of a PrimeScript RT Reagent Kit with gDNA Eraser (Takara), after which the reaction was terminated by incubation at 85°C for 5 s ...
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bioRxiv - Molecular Biology 2019Quote: ... cerevisiae CLN2 was appended to the C-terminal codon of the HIS3 gene using the In-Fusion Cloning Kit (Clontech) as previously described (2) ...
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bioRxiv - Cell Biology 2019Quote: ... PCR fragments containing M1 Spastin amino acids 197-328 was inserted into the C-terminus of FP-M11-92 linearized by BamHI enzyme using In-Fusion cloning kit (Takara). DsRed-M11-92-197-328 was constructed by replacing mApple in mApple-M11-92-197-328 with DsRed2 using NheI and EcoRI restriction sites ...
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bioRxiv - Biochemistry 2023Quote: The full-length SKP1-FBXO22 fusion protein with a 3xGGGS-linker was cloned into a pNic-Bio2 vector that encodes for an N-terminal His-tag as well as a C-terminal Avi-tag (GenBank: JF912191) using an In-Fusion HD Cloning System kit (Takara), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... cells were resuspended in Trizol reagent and frozen at -20°C until RNA was extracted using the Macherey-Nagel Nucleospin RNA XS Kit (CAS: 740902.50, Takara Bio) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... after which it was used as the template for synthesizing cDNA using the PrimeScript™ II 1st strand cDNA Synthesis Kit (Takara Bio, Beijing, China). The full-length ZmCd1 cDNA was amplified by PCR using Phanta Max Super-Fidelity DNA Polymerase (Vazyme Biotech) ...
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bioRxiv - Cell Biology 2022Quote: ... rapalog (A/C Heterodimerizer, Takara), dynapyrazole A (Sigma-Aldrich) ...
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bioRxiv - Biophysics 2021Quote: ... SYBR Premix Ex Taq II (Takara, Otsu, Shiga, Japan) on StepOnePlus Real Time PCR system (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2019Quote: ... using the SYBR premix ExTaq™ II (Takara, Japan) in 96-well optical reaction Roche plates ...
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bioRxiv - Molecular Biology 2019Quote: ... with SYBR Premix Ex Taq II (TaKaRa, Dailian, China). The reaction was carried out at a final volume of 15.0 μL ...
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bioRxiv - Neuroscience 2020Quote: ... with SYBR Premix Ex Taq TM II (Takara Bio). The specificity of amplicons was examined by melting curve analysis and agarose gel electrophoresis ...
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bioRxiv - Plant Biology 2021Quote: ... and TB Green Premix Ex Taq II (Takara, Japan). The following PCR conditions were used ...
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bioRxiv - Immunology 2022Quote: ... using SYBR Premix Ex Taq II mix (TaKaRa Bio) as described [8] ...
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bioRxiv - Plant Biology 2022Quote: ... 10 μL of SYBR Premix Ex TaqTM II (Takara), and 0.4μL of reference dye ...
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Enterohepatic Transcription Factor CREB3L3 Protects Atherosclerosis via SREBP Competitive InhibitionbioRxiv - Physiology 2020Quote: ... and TB Green Premix EX Taq II (TAKARA Bio) (Fujimoto et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... and TB Green Premix Ex Taq II (Takara Bio). Gene expression levels were measured using TaqMan® gene expression assay kit (Applied Biosystems ...
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bioRxiv - Immunology 2021Quote: ... using the SYBR Premix Ex Taq II (Takara, Japan), using a BioRad Chromo4 real-time PCR machine ...
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bioRxiv - Immunology 2020Quote: ... with TB Green Premix Ex Taq II (RR820A; Takara). The primer sequences used in this study are listed in Table 2.
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bioRxiv - Cell Biology 2021Quote: ... or TB Green Premix Ex Taq II (Takara, RR820A) was used according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... using TB Green Premix Ex Taq II (Takara, Japan). The qRT-PCR assays were performed with technical triplicates of three biological replicates ...
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bioRxiv - Immunology 2022Quote: ... or TB Green Premix Ex Taq II (TaKaRa Bio). All data are presented as relative expression levels normalized to Hprt expression ...
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bioRxiv - Microbiology 2022Quote: ... and Thermal Cycler Dice Real Time System II (Takara). All primers used in this study are listed in Table S5 (No ...
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bioRxiv - Microbiology 2022Quote: ... and Thermal Cycler Dice Real Time System II (Takara). Three biologically independent experiments were performed and used for the analysis.
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bioRxiv - Plant Biology 2022Quote: ... 10 μl SYBR Premix Ex Taq II (Takara, Japan), and nuclease-free water ...
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bioRxiv - Molecular Biology 2023Quote: ... on Thermal Cycler Dice Real Time System II (TaKaRa). The relative mitochondrial DNA content normalized by nuclear-genome content was determined using the ΔΔCt method.
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bioRxiv - Genetics 2023Quote: ... and TB Green Premix Ex TaqTM II (Takara, RR820A) in a StepOne real-time PCR system (Applied Biosystems) ...
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bioRxiv - Cell Biology 2023Quote: ... and TB Green Premix Ex Taq II (TaKaRa Bio). In each sample ...
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bioRxiv - Cell Biology 2023Quote: ... using SYBR® Premix Ex Taq™ II (TaKaRa). Specific primers for RPL13 ...
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bioRxiv - Cell Biology 2023Quote: ... and SYBR Premix Ex Taq II (Takara, Shiga, Japan) with gene-specific primers (S4 Table ...
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bioRxiv - Plant Biology 2024Quote: ... 10 µL SYBR Premix Ex Taq II (Takara, China), 1 µL (200 nM final concentration ...
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bioRxiv - Cell Biology 2024Quote: ... using SYBR® Premix Ex TaqTM II (TaKaRa, Japan). All primer information for each target gene was obtained from the Primerbank website(https://pga.mgh.harvard.edu/primerbank/) ...
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bioRxiv - Microbiology 2022Quote: ... The resultant lysate was cleared by centrifugation at 30 000 x g for 20 min and applied to Talon CellThru Co2+ chelating resin (TaKaRa-Clontech). The protein bound resin was washed with 3 column volumes (CV ...
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bioRxiv - Microbiology 2021Quote: ... RNAs were treated for 1 h by DNase I at 37°C and reverse-transcribed using a Prime ScriptTM RT reagent kit with gDNA Eraser (Takara, Japan). Quantitative PCR was performed as mentioned above ...
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bioRxiv - Plant Biology 2021Quote: ... 1 μg of the total RNA from the leaves was reverse-transcribed for 30 min at 42°C in a 20-μl reaction volume using a High Fidelity PrimeScriptTM RT-PCR kit (Takara, Japan) according to the manufacturer’s instructions ...
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Stem cell-derived macrophages as a new platform for studying host-pathogen interactions in livestockbioRxiv - Immunology 2021Quote: ... Filtered lentivirus was either stored in aliquots at −80°C or further purified and concentrated using the Lenti-X Maxi Purification kit (Takara #631234) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... and the PCR product was subcloned into the pcDNA3.1 (+) C-terminal PA vector (digested with KpnI/XhoI) using an In-Fusion HD Cloning Kit (#Z9648N; TaKaRa, Kusatsu, Japan), resulting in pcDNA3.1(+ ...