Mouse Anti Chikungunya Virus E2 Protein 16A12 citations for Takara Bio

Quote: ... and TCR beta chain libraries were generated using SMARTer Mouse TCR a/b Profiling Kit (Clontech). Samples were pooled to a final pool concentration of 4 nM and diluted to a final concentration of 13.5 pM ...

Protective mitochondrial fission induced by stress responsive protein GJA1-20k

Daisuke Shimura, et al., bioRxiv - Cell Biology 2021

Quote: ... for the samples from the mouse heart or Human Mitochondrial DNA Monitoring Primer Set (TaKaRa Bio) for the cells following manufacturer protocol.

Plant mixed lineage kinase domain-like proteins limit biotrophic pathogen growth

Lisa Mahdi, et al., bioRxiv - Plant Biology 2019

Quote: Primary antibodies were monoclonal antibodies from mouse: α-GFP (JL-8, 1:5000, Takara, Shiga, Japan) or rat ...

ATP allosterically stabilizes Integrin-linked kinase for efficient force generation

Isabel M. Martin, et al., bioRxiv - Biophysics 2021

Quote: Full length mouse ILK cDNA was cloned into the EcoRI site of pEGFP-N1 plasmid (Clontech). The R255A ...

Arabidopsis ROOT PHOTOTROPISM2 is a Light-Dependent Dynamic Modulator of Phototropin1

Taro Kimura, et al., bioRxiv - Plant Biology 2019

Quote: Each purified protein preparation of His-PHOT1 N2 and N4 was incubated with TALON Magnetic Beads (TaKaRa) at 4°C for 30 min and further incubated at 4°C for 30 min with in vitro transcription and translation reactant containing RPT2 N ...

Arabidopsis ROOT PHOTOTROPISM2 is a Light-Dependent Dynamic Modulator of Phototropin1

Taro Kimura, et al., bioRxiv - Plant Biology 2019

Quote: ... 30 µg of microsomal proteins were then treated with 30 units of calf intestinal alkaline phosphatase (TaKaRa) at 37°C for 2 h ...

N-terminal ubiquitination of amyloidogenic proteins triggers removal of their oligomers by the proteasome holoenzyme

Yu Ye, David Klenerman, Daniel Finley, bioRxiv - Biochemistry 2019

Quote: The supernatant from purification of His6-tagged proteins was loaded onto a self-packed cobalt column (Clontech). Unbound proteins were washed off with Loading Buffer (50 mM Tris-HCl [pH 7.4] ...

Rational engineering of an erythropoietin fusion protein to treat hypoxia

Jungmin Lee, et al., bioRxiv - Synthetic Biology 2021

Quote: ... Concentrated protein was bound to 0.5–1 mL of His60 nickel or HisTalon cobalt resin (Takara Bio) for 0.5–1 hr at 4°C while rotating in a 10-mL Pierce disposable column (Thermo Scientific) ...

A slow-exchange conformational switch regulates off-target cleavage by high-fidelity Cas9

Viviane S. De Paula, et al., bioRxiv - Biochemistry 2020

Quote: ... Proteins were expressed in Escherichia coli strain BL21(DE3) cells containing chaperone plasmid pG-KJE8 (TAKARA, 3340). When the optical density at 600 nm reached 0.6 ...

An infectivity-enhancing site on the SARS-CoV-2 spike protein is targeted by COVID-19 patient antibodies

Yafei Liu, et al., bioRxiv - Microbiology 2020

Quote: ... the His-tagged spike protein produced in the culture supernatants was purified with a Talon resin (Clontech).

Neisseria gonorrhoeae subverts formin-dependent actin polymerization to colonize human macrophages

Stanimir S. Ivanov, et al., bioRxiv - Microbiology 2021

Quote: U937 cell lines stably expressing red fluorescent protein membrane were generated using pDsRed-Monomer-Mem (Takara Bio) cloned into pLB vector from Addgene (Addgene plasmid 11619 ...

A non-acidic method using hydroxyapatite and phosphohistidine monoclonal antibodies allows enrichment of phosphopeptides containing non-conventional phosphorylations for mass spectrometry analysis

K. Adam, et al., bioRxiv - Biochemistry 2019

Quote: ... 500 µg protein at 1 mg/mL were prepared with Buffer A (Phosphoprotein Kit, Clontech, Cat#635626): 185 µL PC-3 lysate (pH 8.8) ...

PICH function is required for organization of SUMOylated proteins on mitotic chromosomes

Victoria A. Hassebroek, et al., bioRxiv - Cell Biology 2020

Quote: ... The expressed protein was purified using hexa-histidine-tag and GST-tag by Talon-resin (Clontech/Takara) or Glutathione-sepharose (GE healthcare ...

PICH function is required for organization of SUMOylated proteins on mitotic chromosomes

Victoria A. Hassebroek, et al., bioRxiv - Cell Biology 2020

Quote: ... The expressed protein was purified using hexa-histidine-tag and GST-tag by Talon-resin (Clontech/Takara) or Glutathione-sepharose (GE healthcare ...

Chemical screening pipeline for identification of specific plant autophagy modulators

Adrian N. Dauphinee, et al., bioRxiv - Plant Biology 2019

Quote: ... Proteins were transferred onto a PVDF membrane and blotted using 1:2000 a-GFP (Cat 632381, Clontech), 1:2000 a-actin (AS13 2640 ...

Ameliorative effect of a quinazoline-based bromodomain inhibitor, CN210, on experimentally-induced Crohn's disease-like murine ileitis via inhibition of inflammatory cytokine expression

Takehisa Noguchi, et al., bioRxiv - Pharmacology and Toxicology 2020

Quote: ... and TATA-binding protein (TBP) (MA050367) were obtained from the Perfect Real-Time Supporting System (Takara Bio). The mRNA expression level was standardised to that of TBP ...

Ameliorative effect of a quinazoline-based bromodomain inhibitor, CN210, on experimentally-induced Crohn's disease-like murine ileitis via inhibition of inflammatory cytokine expression

Takehisa Noguchi, et al., bioRxiv - Pharmacology and Toxicology 2020

Quote: ... and TATA-binding protein (TBP) (MA050367) were obtained from the Perfect Real-Time Supporting System (Takara Bio). The mRNA expression level was standardised to that of TBP.

Distal-less genes Dlx1/Dlx2 repress oligodendrocyte genesis through transcriptional inhibition of Olig2 expression in the developing vertebrate forebrain

Qiang Jiang, et al., bioRxiv - Developmental Biology 2020

Quote: ... Both chicken DLX1 and DLX1 Q50E proteins were purified using Capturem His-Tagged Purification Maxiprep Kit (Clontech), and verified by Western blot with primary antibodies against 6x His tag (Invitrogen ...

Metabolic heritage mapping: heterogenous pools of cytoplasmic nucleotide sugars are selectively utilized by various glycosyltransferases

Paulina Sosicka, et al., bioRxiv - Biochemistry 2021

Quote: ... After 12 h medium was collected and recombinant proteins were purified on TALON metal affinity resin (Takara). 200 μl of the resin was loaded onto 1 ml column (Bio-Rad ...

RNA and FUS act in concert to prevent TDP-43 spatial segregation

Clément Demongin, et al., bioRxiv - Molecular Biology 2023

Quote: ... cDNAs encoding for sequences of proteins of interest were amplified and inserted into pEGFP-N1 vector (Clontech). Each DNA construct was checked by conventional Sanger sequencing of purified plasmid.

Yeast PRPP amidotransferase assembles into fine granules to promote de novo purine synthesis through intermolecular substrate channeling

Masak Takaine, Rikuri Morita, bioRxiv - Cell Biology 2023

Quote: ... The concentration of the purified protein was assessed by densitometry using bovine serum albumin (TaKaRa, cat. # T9310A) as a standard following SDS-PAGE and staining with the Q-stain ...

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