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Citations for Takara Bio :
551 - 600 of 1168 citations for Mouse AWAT2 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2022Quote: ... 56 plasmids in 293FT HEK cells using the CalPhos™ Mammalian Transfection Kit (Takara bio, 631312). Following 2 μM tamoxifen treatment ...
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bioRxiv - Developmental Biology 2023Quote: ... This rtTA-advanced ORF was replaced with rtTA3G from the pCMV-Tet-3G plasmid (TaKaRa Bio), by XbaI and XmaI digestion and cloning of three separate fragments to create the final rtTA vector AAVS1-NEO-UBrtTA-3G.
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bioRxiv - Genomics 2023Quote: ... Recombinant AAV2 was packaged in HEK293T cells using pHelper and pRC2-mi342 plasmids (Takara, Catalog #632608). Three days after transfection ...
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bioRxiv - Molecular Biology 2024Quote: ... YCplac22 plasmids that carry tsa1 mutant alleles were constructed using the PrimeSTAR Mutagenesis Basal Kit (TaKaRa) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Retrovirus plasmids were generated by restriction enzyme cloning of latency genes into pRetroX-IRES-ZsGreen (Takara). Latency locus genes were amplified from WT MHV68-BAC DNA127 and the frame-shift stop mutant ...
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bioRxiv - Bioengineering 2023Quote: ... Plasmid DNA from the Gibson assembly reaction was then transformed into Stellar Competent Cells (Takara Bio).
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bioRxiv - Biochemistry 2023Quote: The plasmids for producing XccOpgD mutants were constructed using a PrimeSTAR mutagenesis basal kit (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: HEK293T cells were transfected with expression plasmids using the TransIT-293 transfection reagent (Takara, Shiga, Japan), following the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... Plasmid sequences confirmed by Sanger sequencing (Quintara Biosciences) were transformed into Stellar Competent Cells (Takara Bio) and purified using the NucleoBond Xtra Midi endotoxin-free midi-prep kit (Takara Bio) ...
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bioRxiv - Developmental Biology 2024Quote: ... Col2a1a enhancer was amplified from the plasmid -1.7kbcol2a1a:EGFP-CAAX (24) using CloneAmp HiFi premix (Takara #639298) with the primers ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-P-cadherin (TAKARA Cat#M127, 1:100 for IF); mouse anti-Rac1 (Abcam Cat#ab33186 ...
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bioRxiv - Cell Biology 2020Quote: Mouse monoclonal E-cadherin antibody (HECD-1) was purchased from Takara Bio Inc ...
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bioRxiv - Neuroscience 2021Quote: ... anti-human GFAP (mouse IgG1, 1:2000, Takara Bio, Shiga, Japan), anti-CNPase (mouse IgG1 ...
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bioRxiv - Microbiology 2019Quote: ... purchased antisera used were: mouse monoclonal anti-GFP (clone JL8, Clontech), rat monoclonal anti-ß tubulin (clone YL1/2 ...
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bioRxiv - Genetics 2019Quote: ... GAL4 DNA-BD Mouse Monoclonal Antibody (Takara Bio USA, Inc. #630403), and GAPDH Mouse Monoclonal Antibody (CB1001-500UG 6C5 ...
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bioRxiv - Biochemistry 2019Quote: ... Western blotting was performed with anti-6×His mouse mAb (Clontech), with secondary IRDye 800CW goat anti-mouse (LI-COR ...
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bioRxiv - Neuroscience 2020Quote: ... monoclonal mouse anti-STEM121 (1:500; Y40410, Takara, Mountain View, CA), polyclonal rabbit anti-GFAP (1:500 ...
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bioRxiv - Microbiology 2021Quote: ... Gal4-based two-hybrid screening against the mouse cDNA library (Clontech) was performed as described before (Mitsuzawa et al. ...
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bioRxiv - Cell Biology 2021Quote: ... Monoclonal mouse anti-GFP antibodies (JL-8) were from Clontech (632381). Monoclonal mouse anti-Flag (M2 ...
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bioRxiv - Immunology 2022Quote: ... mouse anti-GFP clone JL-8 (Takara Bio Cat# 632380, RRID:AB_10013427), rabbit anti-FLAG clone D6W5B (Cell Signaling Technology Cat# 14793 ...
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bioRxiv - Developmental Biology 2022Quote: ... Predicted enhancer elements were PCR-amplified from mouse genomic DNA (Clontech) and cloned into the respective LacZ expression vector (Osterwalder et al ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated with primary antibodies: mouse anti-GFP (1:2000; Clontech), mouse anti-FLAG (1:1000 ...
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bioRxiv - Genetics 2021Quote: ... probed with anti-GFP (Takara Bio Clontech, #632380, mouse, 1:5000) and anti-Tubulin (Sigma ...
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bioRxiv - Genetics 2021Quote: ... probed with anti-GFP (Takara Bio Clontech, #632380, mouse, 1:5000) and anti-Tubulin (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... Primary anti-6xHis monoclonal mouse antibody (Clontech, 631212, diluted 1:1,000) was used to detect 6xHis-ubiquitin in samples permeabilized with 0.5% Triton-X100 ...
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bioRxiv - Developmental Biology 2020Quote: ... Westerns were probed with mouse monoclonal anti-GFP JL8 (#632381, Clontech) or mouse monoclonal anti-Myc 9E10 (DSHB).
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bioRxiv - Cell Biology 2020Quote: ... Living colors mouse anti-GFP monoclonal antibody was purchased from Clontech, and goat anti-mouse antibody HRP-conjugated antibody was purchased from Jackson ImmunoResearch.
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bioRxiv - Bioengineering 2022Quote: ... EGFP was detected using the JL-8 mouse monoclonal antibody (Clontech). For IHC ...
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bioRxiv - Neuroscience 2023Quote: ... mouse anti-mCherry monoclonal antibody (1:500; Takara Bio Cat# 632543), Alexa Fluor 488-conjugated anti-chicken IgG antibody (1:500 ...
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bioRxiv - Plant Biology 2023Quote: ... Anti-GFP (monoclonal antibody raised in mouse, Takara, USA, catalog # 632381) and anti-SEC12 (Bar-Peled and Raikhel ...
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bioRxiv - Neuroscience 2023Quote: ... primary mouse cortical neurons were transfected with pEGFP-C1 vector (Clontech) at 3 days in vitro (DIV ...
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A gene desert required for regulatory control of pleiotropic Shox2 expression and embryonic survivalbioRxiv - Developmental Biology 2023Quote: ... Predicted enhancer elements were PCR-amplified from mouse genomic DNA (Clontech) and cloned into a Hsp68-LacZ expression vector for random integration114 ...
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bioRxiv - Cell Biology 2023Quote: ... A cDNA library generated from mouse placenta total mRNA (Takara, 636672) served as template to amplify the domains by PCR ...
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bioRxiv - Biochemistry 2020Quote: ... pGAD plasmids were transformed into PJ49-4a yeast and were selected on leucine-dropout medium (Clontech, #630414). pTEF or pTEFdual plasmids were included to express proteins in trans and were transformed with pOBD plasmids and selected on media lacking tryptophan and uracil (Sunrise Science Products ...
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bioRxiv - Evolutionary Biology 2021Quote: ... All RNA clones were prepared from the plasmids by in vitro transcription with T7 RNA polymerase (Takara) after digestion with Sma I (Takara) ...
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bioRxiv - Molecular Biology 2020Quote: ... The 3xFlag sequence was then ligated into the digested eGFP-Usp9x plasmid (Takara DNA ligation kit #6023).
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bioRxiv - Cancer Biology 2019Quote: ... BirA(R118G)-HA from pcDNA3.1 MCS-BirA(R118G)-HA plasmid was cloned into pRetroX-Tight-Pur (Clontech) at EcoRI site by PCR to get pRetroX-MCS-BioID-HA vector ...
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bioRxiv - Microbiology 2019Quote: ... Subsequently each guide was inserted into the BtgZI linearized pCas/sg plasmid by In-Fusion cloning (Takara) using primers fwd-TTACAGTATATTATT(N20)GTTTTAGAGCTAGAA and rev-TTCTAGCTCTAAAAC(N20)AATAATATACTGTAA ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The plasmid GFP-GT198 contained full-length human GT198 in a pEGFP-C3 vector (Clontech, 6082-1). HeLa cells transfected with GFP-GT198 (green ...
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bioRxiv - Cell Biology 2019Quote: The plasmid pDendra2NatMX was constructed by ligation of the NheI-HpaI Dendra2 fragment from pDendra2-C (Clontech) and the PvuII linearized pAG25 vector (Addgene) ...
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bioRxiv - Biophysics 2019Quote: ... The mEos3.2 tagged constructs used here are in Clontech N1 plasmid vector background (Clontech, Mountain View, CA).
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bioRxiv - Evolutionary Biology 2021Quote: ... pre-amplified (DFRS empty plasmid primers) and pre-digested by the same enzymes (In Fusion Kit; Clontech). The miR-137 or miR-122 complementary sequences were thereby located on the 3’UTR of the RFP gene.
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bioRxiv - Biochemistry 2020Quote: ... Proteins were expressed in Escherichia coli strain BL21(DE3) cells containing chaperone plasmid pG-KJE8 (TAKARA, 3340). When the optical density at 600 nm reached 0.6 ...
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bioRxiv - Cell Biology 2021Quote: ... Slc37a2 was amplified by PCR from BMMCs cDNA and inserted into a plEGFP-C1 plasmid (Takara Bio). Slc37a2 plEGFP-C1 plasmid or plasmid vector control were transfected into Amphopack-293 cells (BD Biosciences ...
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bioRxiv - Neuroscience 2020Quote: ... This sequence was inserted into a donor plasmid (pCRII-TOPO) using In-Fusion HD cloning (Takara – 639649). For the Cas9 plasmid ...
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bioRxiv - Microbiology 2020Quote: ... cerevisae: All Yeast-two hybrid plasmids were generated on the basis of the Matchmaker III System (Clontech Laboratories Inc. ...
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bioRxiv - Biochemistry 2022Quote: ... coding regions were cloned downstream of the CMV promoter in derivatives of the pEGFP-N1 plasmid (Clontech) with an NLS and linker sequences to EGFP or mCherry (Supplemental Table 2) ...
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bioRxiv - Molecular Biology 2022Quote: ... The plasmid DNA bearing the chaperone combination GroEL/ES (pGro7) or DnaK/J/GrpE (pKJE) from Takara Biotech chaperone kit [Nishihara et al. ...
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bioRxiv - Cell Biology 2022Quote: ... Immortalization of the cells was achieved by lipofection of the pCDNA-3xHA-hTERT plasmid with Xfect (Takara BIO INC ...
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bioRxiv - Neuroscience 2020Quote: ... The knock-in plasmid was constructed by using In-Fusion HD Cloning kit (Takara Bio USA #639650) or NEBuilder HiFi DNA Assembly kit (New England Biolabs #M5520 ...