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Citations for Takara Bio :
651 - 700 of 2075 citations for Monoisononyl Phthalate 100 Ug Ml In Mtbe Ring 1 2 13C2 Dicarboxyl 13C2 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... and 2 µl of the reaction was transformed into 25 µl of Stellar chemically competent bacteria (Takara). Plasmid minipreps were performed using the NucleoSpin Plasmid Transfection-grade Mini kit (Macherey-Nagel ...
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bioRxiv - Genomics 2022Quote: ... and 2 µl of the reaction was transformed into 25 µl of Stellar chemically competent bacteria (Takara).
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bioRxiv - Cell Biology 2022Quote: ... full-length GIP-2 cDNA with a C-terminal V5-His6 tag was inserted into pColdI (TAKARA) and used to inoculate rabbits and rats ...
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bioRxiv - Biochemistry 2023Quote: ... The virus particles were further concentrated at 10X in volume using Lenti-X-concentrator (Clontech, PT4421-2) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Yeast 2-hybrid (Y2H) analysis was performed using the protocols described in the Yeast protocols handbook (Clontech, Protocol No ...
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bioRxiv - Molecular Biology 2024Quote: ... Amplification was performed using the Advantage 2 Polymerase Mix (Clonetech, now Takara Bio USA, Mountain View, CA) and the following primers ...
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bioRxiv - Bioengineering 2024Quote: ... Viral genome number was quantified by real-time PCR with AAVpro Titration Kit Ver.2 (Takara, 6233) according to manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2021Quote: ... residues 1-217) and CrkII (Uniprot P46108-1; residues 1-330) were each cloned into a modified pCOLD IV vector (Takara) that contains a N-terminal His TEV cleavage tag by Genscript ...
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bioRxiv - Biochemistry 2022Quote: ... 1-206) and SYCE3 (1-88) were cloned into pGBKT7 vectors (Clontech) and human sequences for SYCP1 (1-811) ...
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bioRxiv - Neuroscience 2022Quote: ... rat anti-GFP (1:200;) and rabbit anti-dsRed (1:600; Clontech). The anti-GFP and anti-dsRed antibodies were used to amplify the intrinsic fluorescent signals in the Ccr2RFP/+fmsEGFP/+ mice ...
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bioRxiv - Immunology 2024Quote: ... TALON metal affinity resin (Takara, #635653, 0.5-1:1 v/v ratio) was washed with PBS and added to the supernatant ...
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bioRxiv - Plant Biology 2019Quote: ... The first-strand cDNA for qRT-PCR was synthesized from 100 ng total RNA using PrimeScript RT reagent Kit with gDNA Eraser (TaKaRa). For qRT-PCR ...
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bioRxiv - Cancer Biology 2019Quote: ... we performed 30 separate 100 μL PCR reactions with 10 μg genomic DNA in each reaction using ExTaq DNA Polymerase (Clontech) then combined the resultant amplicons ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA was annealed to primers by the addition of 4 µL 100 µM Random Hexamer Primers (TaKaRa, Mountain View, CA) and incubation at 65°C for 5 min ...
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bioRxiv - Developmental Biology 2021Quote: ... cDNA was synthesized and amplified from 100 pg–300 pg of input RNA using SMART-seqTMv4 Ultra Low input RNA kit (Takara Clontech). Sequencing libraries were prepared using the Nextera XT DNA library preparation kit (Illumina) ...
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bioRxiv - Plant Biology 2021Quote: ... total RNA was extracted from approximately 100 mg fresh weight (FW) of Arabidopsis shoots using NucleoSpin RNA Plant (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 100 ng RNA extracted from 12 male or female pharate larvae was reverse-transcribed using PrimeScript II reverse transcriptase (TaKaRa) at 50°C for 30 min ...
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bioRxiv - Genetics 2021Quote: ... The fragment length of PCR products was detected by 1.2% agarose gel electrophoresis using 5 μl of 100 bp DNA Ladder (TaKaRa, Japan) and gel stain (TransGen Biotech ...
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bioRxiv - Immunology 2020Quote: ... Filtered viral supernatants were concentrated 100 times using Lenti-X™ Concentrator (Takara Bio, Clontech, Saint Germain en Laye, France) and resuspended in phenol red-free medium ...
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bioRxiv - Immunology 2022Quote: ... Concentrated particles were resuspended in 100% WRN conditioned media and titers were checked using Lenti-XTM GoStixTM Plus (Takara 631280). Only high titer lenti-particles were used to transduce duodenoids ...
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bioRxiv - Microbiology 2022Quote: ... The resulting mutant BAC was electroporated into NEB 10-beta cells and purified using the Nucleobond BAC 100 kit (Takara).
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bioRxiv - Microbiology 2023Quote: ... 50 mM KCl, 100 mM Tris-HCl [pH 7.4], 40% glycerol, 0.4 U/μL Recombinant RNase Inhibitor [TaKaRa, Cat# 2313A]) as described previously [27] ...
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bioRxiv - Neuroscience 2023Quote: ... the RNA concentration was determined via Nanodrop and up to 100 ng was used as an input material for library preparation using the Clontech Ultra Low kit (Clontech).
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bioRxiv - Genomics 2023Quote: ... were used for the genomic DNA (gDNA) extraction with NucleoBond AXG 100 or AXG 500 with NucleoBond Buffer Set IV (Clontech) or DNeasy Blood and Tissue kit (Qiagen) ...
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bioRxiv - Cancer Biology 2023Quote: ... Growth media was changed the following day and lentiviruses-containing supernatants were harvested at 72 hr after transfection and concentrated 100-fold using Lenti-X concentrator (Clontech). For infection ...
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bioRxiv - Microbiology 2023Quote: ... 50 mM KCl, 100 mM Tris-HCl [pH 7.4], 40% glycerol, and 0.4 U/μL Recombinant RNase Inhibitor [TaKaRa, Cat# 2313A]) [27] ...
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bioRxiv - Cell Biology 2023Quote: ... and varying amounts (0, 10, 20, 100, 200 ng) of pAAV MyoD expression vectors into AAVpro 293T cells (Takara Bio) using the FuGENE HD transfection reagent (Promega) ...
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bioRxiv - Neuroscience 2020Quote: ... dsRed (1:1000, Clontech), GFP (1:1000 ...
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bioRxiv - Genomics 2021Quote: ... 1 mM dNTPs (Clontech), 1 U/mL RNase inhibitor ...
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bioRxiv - Neuroscience 2020Quote: ... STEM121 (1:200, Takara), TH (1:200 ...
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bioRxiv - Molecular Biology 2022Quote: ... AP21967 (1 μM, TaKaRa).
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bioRxiv - Neuroscience 2023Quote: pmCherry-1 (632525, Clontech) was used as the backbone and control vector for the overexpression experiments ...
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bioRxiv - Genetics 2024Quote: ... 1 mM dNTPs (Clontech), 1 U/mL RNase inhibitor ...
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bioRxiv - Immunology 2021Quote: ... directly into 1.7 ml eppendorf tubes containing 12.0 μl 1X reaction buffer (Takara) for cDNA synthesis using the SMART-Seq v4 Ultra low Input RNA kit (Takara ...
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bioRxiv - Plant Biology 2020Quote: ... and 200 ng/mL AbA (Takara Bio USA, Inc., Mountain View, US-CA) were added when they are required ...
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bioRxiv - Cell Biology 2022Quote: ... transfected iPSCs were selected for 48 hours with 0.5 μg/mL puromycin (Takara).
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bioRxiv - Synthetic Biology 2022Quote: ... A total of 5 ml of Talon Metal Affinity Resin (Takara Bio USA) was added to the supernatant and mixed overnight at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... Diluted lysate was incubated with 1.25 ml His60 Ni Superflow affinity resin (Takara) per liter of expression culture ...
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bioRxiv - Developmental Biology 2019Quote: ... the 10 mL of viral supernatant were concentrated with LentiX Concentrator (Takara, 631231) due to the lower viral titer (Datlinger et al ...
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bioRxiv - Biochemistry 2021Quote: ... and incubated with 0.5 mL nickel resin (His60 Ni Superflow Resin, Takara Bio) in a Poly-Prep chromatography column (BioRad ...
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bioRxiv - Immunology 2021Quote: ... were coated overnight at 4°C with 20 μg/ml Retronectin (Takara T100B). Viral supernatant was spun for 90 minutes at 2,000g and 30°C onto the plated retronectin and half the supernatant volume removed carefully after spinning ...
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bioRxiv - Microbiology 2023Quote: ... The sample was loaded onto a 5-ml TALON metal affinity resin (Clontech) equilibrated in loading buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... and the supernatant was incubated with 4 ml TALON metal affinity resin (Clontech) at 4°C for 1 hr ...
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bioRxiv - Immunology 2022Quote: ... coated with 30 μg/mL Retronectin reused up to three times (T100B, Takara), at 2000 x g for 2 hours at 32°C and replaced with activated human CD8+ T cells in fresh RPMI supplemented with 30 U/mL IL-2 ...
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bioRxiv - Cancer Biology 2023Quote: ... Clarified lysate was mixed with ∼0.5 ml/L of culture cobalt resin (TaKaRa) for one hour ...
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bioRxiv - Immunology 2023Quote: ... retronectin-treated plates were prepared by coating with 12.5 μg/ml retronectin (TaKaRa) in PBS (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... transfected iPSCs were selected for 48 hours with 0.5 μg/mL puromycin (Takara). Differentiation of iPSCs into iNeurons was performed using an established protocol35 ...
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bioRxiv - Cancer Biology 2024Quote: ... Untreated tissue culture plates were coated with 25mg/ml Retronectin (Takara Bio #T100B) overnight at 4°C to be used for retroviral transduction ...
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bioRxiv - Biochemistry 2024Quote: ... The clarified supernatant was incubated with 5 ml of TALON resin (Takara Bio) for 90 min at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... SLD3 and ESA1 ORF were PCR-amplified with Taq polymerase (MightyAmp ver. 2, Takara Bio Co., Otsu, Japan) and cloned into the BamHI sites of the Y2H DB vector pST1667 and the Y2H AD vector pST548 (Tanaka ...