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Citations for Takara Bio :
1 - 50 of 4835 citations for Medium Kit without Serum and with CultureBoost since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2019Quote: ... Then yeast cells were plated on SD/-LW medium (minimal media double dropouts, SD basal medium without Leu and Trp) according to the manufacturer’s protocol (Clontech, USA) for 72 h ...
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bioRxiv - Plant Biology 2023Quote: ... The yeast transformants were grown on nutrient-restricted (without Trp, Leu, His, Ade) mediums (Clontech) 3-5 days to assess interactions between various protein combinations.
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bioRxiv - Molecular Biology 2023Quote: ... medium supplemented with 10% Fetal bovine serum (FBS, Clontech). One well from a 6-well-plate at 70% confluency was used for the transfection ...
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bioRxiv - Microbiology 2022Quote: ... Transformed yeast cells were cultured in a synthetic-defined (SD) medium without tryptophan (SD/-Trp broth, Takara Bio USA Inc.) and induced in SD/-Trp broth with 2% raffinose as an alternative carbon source by the addition of 1 to 3% galactose ...
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bioRxiv - Plant Biology 2023Quote: ... were used to co-transform yeast strain AH109 and colonies carrying both vectors were selected on SD medium without tryptophan (-W) and leucine (-L) (TaKaRa 630317) at 28°C ...
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bioRxiv - Molecular Biology 2022Quote: Stable clones were grown in medium containing Tet system-approved fetal bovine serum (TaKaRa Bio), puromycin ...
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bioRxiv - Plant Biology 2022Quote: ... without DNA contamination upon DNase I treatment (Takara). After quality control (OD26/280=1.8-2.2 ...
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bioRxiv - Microbiology 2020Quote: ... the target genes with or without glycosite mutation were cloned into the pTOR::FLAG vector using the In-fusion HD Cloning kit (Clontech, Beijing, China) (Table S1) ...
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bioRxiv - Cell Biology 2021Quote: ... HUVECs were maintained in Endothelial Cell Basal Medium 2 supplemented with Endothelial Cell Growth Medium kits (C22211, C22111, Takara). For replating cells ...
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bioRxiv - Systems Biology 2020Quote: ... The cell lines were cultured in IMDM medium (Quality Biological) supplemented with 10% (v/v) Tet System Approved fetal calf serum (Takara Bio/previously Clontech), 100 U/mL penicillin ...
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bioRxiv - Cancer Biology 2022Quote: ... then cDNA was synthesized without RNA purification using SMART-seq HT (Takara). For the PANC-1 cells ...
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bioRxiv - Microbiology 2022Quote: ... the cells were treated with or without 1 μM rapalog (Takara Bio) for one hour prior to fixation ...
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bioRxiv - Molecular Biology 2022Quote: ... the transfection medium was replaced with 2 ml of reduced serum culture medium (5% FCS) supplemented with 300 μM A/C heterodimerization agent (formerly AP21967, Takara BioInc), 20 mM HEPES and 10 μM cholesterol (balanced with methyl-β-cyclodextrin ...
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bioRxiv - Biophysics 2020Quote: ... was cultured under the following conditions: cells were maintained in DMEM/F-12 50/50 medium containing 10% fetal bovine serum (tetracycline-free, Takara, Mountain View, CA), penicillin (100 units/ml) ...
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bioRxiv - Systems Biology 2020Quote: ... The cell lines were cultured in IMDM medium (Quality Biological) supplemented with 10% (v/v) Tet System Approved fetal calf serum (Takara Bio/previously Clontech), 100 U/mL penicillin ...
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bioRxiv - Cell Biology 2022Quote: ... with or without 1.59 nM (50 μg/μL) bacteriophage λ DNA (48502 bp; TaKaRa). The ratio between condensin II and λ DNA was 1 condensin II ...
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bioRxiv - Molecular Biology 2019Quote: ... Circulating miRs were isolated from 300 µL serum by using a NucleoSpin microRNA isolation kit (TaKaRa, Otsu, Japan).
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bioRxiv - Molecular Biology 2020Quote: A549 cells were obtained from ATCC and cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS, Takara Biomedical Technology Co., Ltd., China), 100 U/ml penicillin and 100 U/ml streptomycin (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... RNA was purified from the medium with NucleoSpin® RNA Virus kit (U0956A, Takara Bio Inc.) by following the manufacture’s instruction and subjected to RT-qPCR with the primer pairs for EGFP (5′-CAAGCTGACCCTGAAGTTCATCTG-3′ and 5′-TTGAAGAAGTCGTGCTGCTTCATG-3′ ...
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bioRxiv - Plant Biology 2022Quote: The BRI1-coding region without the stop codon was cloned into pMD19-T (simple) (Takara Biotechnology) to generate pMD19-BRI1 that was used as a template to generate the mutants BRI1Y599F ...
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bioRxiv - Neuroscience 2023Quote: ... pAcGFP-His-MAP2C without AcGFP was amplified and Dendra2 was amplified from pDendra2-C vector (Takara). Dendra2 was inserted into where AcGFP was by In-Fusion cloning kit ...
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bioRxiv - Cell Biology 2023Quote: ... neurons expressing the indicated constructs were incubated with or without 100 nM linker (AP21967; Takara Cat#635056) for 2 h prior to fixation and imaging.
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bioRxiv - Developmental Biology 2023Quote: ... YPD medium (Clontech), and Potato Dextrose Broth (Sigma-Aldrich) ...
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bioRxiv - Synthetic Biology 2021Quote: ... and 10% Fetal Bovine Serum (Clontech). Cells were grown at 37°C with 5% CO2 and passaged using 0.05% Trypsin-EDTA solution (Thermo Fisher ...
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bioRxiv - Neuroscience 2021Quote: ... cells and medium were collected and AAV particles were purified by AAVpro Purification Kit (Takara Bio; Shiga, Japan) according to the manufacturer’s instructions.
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bioRxiv - Systems Biology 2020Quote: ... Fibroblast medium was replaced by neural differentiation medium (NDiff227; Takara-Clontech) supplemented with growth factors at the following concentrations ...
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bioRxiv - Systems Biology 2020Quote: ... Fibroblast medium was replaced by neural differentiation medium (NDiff227; Takara-Clontech) supplemented with growth factors at the following concentrations ...
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bioRxiv - Developmental Biology 2024Quote: ... PXGL or UXGL medium was made from N2B27 medium (Takara, Y40002), supplemented with either 1 µM PD0325901 (Cambridge Bioscience ...
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bioRxiv - Biochemistry 2020Quote: One colony was used to inoculate 10mL of selection media (6.9 g/L Yeast Nitrogen Base without amino acids, 0.62g/L Clontech -Leu/-Trp/-Ura dropout supplement ...
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bioRxiv - Biochemistry 2020Quote: ... Transformed cells were plated on a selection agar (6.9g/L yeast nitrogen base without amino acids, 0.62g/L Clontech -Leu/-Trp/-Ura dropout supplement ...
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bioRxiv - Cell Biology 2022Quote: ... the medium was exchanged with primate ES Cell Medium containing doxycycline (TaKaRa) (1.0 μg/mL) ...
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bioRxiv - Immunology 2021Quote: Cell death was assessed by the release of LDH in conditioned medium using LDH Cytotoxicity Detection Kit (TaKaRa, CA).
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bioRxiv - Immunology 2021Quote: Cell death was assessed by the release of LDH in conditioned medium using LDH Cytotoxicity Detection Kit (TaKaRa, CA). IL-1β levels in conditioned media were measured by ELISA (eBiosciences ...
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bioRxiv - Microbiology 2023Quote: ... The p24 amount in the medium was quantified by Lenti-X p24 Rapid Titer Kit (632200, Takara Bio Inc.) with the p24 control samples ...
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bioRxiv - Cell Biology 2023Quote: Cell death was assessed by the release of LDH in conditioned medium using LDH Cytotoxicity Detection Kit (TaKaRa, CA). IL-1β levels in conditioned media were measured by an ELISA kit (eBiosciences ...
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bioRxiv - Molecular Biology 2019Quote: ... with 10% Fetal Bovine Serum (FBS, Takara) and 100 U/mL penicillin/streptavidin (pen/strep ...
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bioRxiv - Cell Biology 2019Quote: ... with bovine serum albumin (BSA) standard (Takara). The antibodies used in this research are described Table S2.
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bioRxiv - Microbiology 2020Quote: ... ramulus DSM 15684 were cloned without signal peptide or transmembrane domain from genomic DNA using In-Fusion cloning (Takara) and the primers in Table S8 into the EcoRI and Ncol restriction sites of the corresponding plasmids ...
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bioRxiv - Cancer Biology 2020Quote: ... Both CnAOEC and ISO-HAS-B were cultured in Endothelial Cell Growth Medium 2 Kit (Takara Bio, Inc. Kusatsu, Japan). All cells used were routinely tested for Mycoplasma using PCR and were submitted to ICLAS Monitoring Center (Kawasaki ...
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bioRxiv - Cell Biology 2023Quote: cfDNA in the conditioned cell culture medium was extracted using NucleoSpin Gel and PCR Clean-up kit (Takara, Duren, Germany), and quantified with Nanodrop (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2020Quote: The coding sequence of PhS3-RNase (without signal peptide) as well as its six mutant forms described above were separately cloned into pCold-TF vector (Takara). Relevant primer sequences are listed in Supplemental Table 3 ...
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bioRxiv - Microbiology 2019Quote: ... mIRE1-wt was PCR-amplified (without a myc tag) from pcDNA3-mIRE1-3xmyc (Stahl et al, 2013) and subcloned in pMSCVhyg (Clontech) using BglII and HpaI sites ...
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C53 interacting with UFM1-protein ligase 1 regulates microtubule nucleation in response to ER stressbioRxiv - Cell Biology 2020Quote: ... The complete sequence of CDK5RAP3 without the stop codon was excised from pCR-hCDK5RAP3 by NheI/SalI and ligated into pEGFP-N3 (Clontech), resulting in plasmid phCDK5RAP3-EGFP ...
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bioRxiv - Microbiology 2021Quote: Expression plasmids encoding for human ZMPSTE24 with and without a C-terminal FLAG-tag or HA-tag were PCR amplified and subcloned into the pQXCIP (Clontech) backbone using flanking restriction sites AgeI and BamHI ...
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bioRxiv - Cell Biology 2021Quote: ... CPE 451 mCherry constructed by Gibson assembly by sub-cloning the 451 amino acids of CPE (without the Amphipathic Helix) into pmCherry-N1 (Clontech) vector using XhoI and BamHI restriction sites.As control vectors we used pEGFP-N3 and pmCherry-N1.All constructs were sequenced to confirm the fidelity of the process ...
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bioRxiv - Plant Biology 2022Quote: ... backbone with the FLAG and SV40 NLS (for version1) or without the FLAG and SV40NLS (for version 2) were amplified and assembled by TAKARA in-fusion HD cloning kit (cat639650 ...
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bioRxiv - Cell Biology 2023Quote: ... CMV-mCherry-SV40-PA was amplified via PCR and the OLIGO273 and OLIGO274 from p-mCherry-N1 without multiple cloning site (modified Clontech, Takara; United States ...
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bioRxiv - Cell Biology 2023Quote: ... CMV-mCherry-SV40-PA was amplified via PCR and the OLIGO273 and OLIGO274 from p-mCherry-N1 without multiple cloning site (modified Clontech, Takara ...
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bioRxiv - Synthetic Biology 2019Quote: ... supplemented with 10% FBS (fetal bovine serum, Clontech) and 1% P/S (Penicillin-Streptomycin ...
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bioRxiv - Molecular Biology 2019Quote: ... supplemented with 10% fetal bovine serum (FBS) (Takara), 1% MEM Non-Essential Amino Acids Solution (NEAA 100X ...