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Citations for Takara Bio :
51 - 100 of 202 citations for Measles Virus Nucleoprotein HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Collected crude virus was concentrated with Lenti-X (Takara Bio) following manufacturer instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Virus was concentrated with polyethylene glycol (Lenti-X, Takara, #631232). Lentivirus was produced in airway epithelial cell expansion medium supplemented with Y27632 and retinoic acid as described95 ...
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bioRxiv - Microbiology 2021Quote: ... Complete nucleoprotein and glycoprotein genes were amplified using Takara long amplicon Taq polymerase with GC buffers (RR02AG Takara Bio USA, Mountain View, CA, USA) using the primers indicated in Table 1 after cDNA synthesis using random hexamer primers and Roche AMV reverse transcriptase (10109118001 Roche ...
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bioRxiv - Plant Biology 2020Quote: ... Using the cDNA synthesis kit (TaKaRa: Moloney Murine Leukemia Virus Version), cDNA was synthesized from extracted RNA ...
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bioRxiv - Cancer Biology 2020Quote: ... The titer of virus was measured using P24 ELISA kit (Clontech).The virus was aliquoted and stored at −80°C.
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bioRxiv - Molecular Biology 2020Quote: ... virus supernatant was concentrated with Lenti-X™ Concentrator (Takara, #631231) as per the manufacturer’s protocol.
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bioRxiv - Biochemistry 2020Quote: ... Virus titer was determined using Lenti-XTM GoStixTM Plus (Takara Bio), and Huh7 cells were infected at a MOI of 5 ...
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bioRxiv - Genetics 2022Quote: ... Virus titer was estimated using Lenti-X GoStix Plus (Takara, 631281) after 100x dilution ...
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bioRxiv - Immunology 2023Quote: ... The virus titer was determined using Lenti-XTM GoStix Plus (Clontech).
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bioRxiv - Cell Biology 2024Quote: ... The virus titer was determined using Lenti-X GoStix Plus (Clontech). To transduce hTCEpi cells ...
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bioRxiv - Developmental Biology 2024Quote: ... Foamy virus was produced using Lenti-X 293T (Takara Bio, Kusatsu) generated as previously described and frozen at -80°C for long term storage42 ...
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bioRxiv - Neuroscience 2023Quote: Rabies genomes were exacted by NucleoSpin® RNA Virus (740956.50, Takara), RT-PCR was performed with AccuScript PfuUltra II RT-PCR Kit (600184 ...
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bioRxiv - Physiology 2024Quote: ... Virus concentration was determined using Lenti-X GoStix Plus (Takara Bio) after one freeze-thaw cycle to be consistent with experimental conditions.
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bioRxiv - Molecular Biology 2024Quote: ... separated by an encephalomyelitis virus internal ribosome entry site (IRES) (Clontech), were cloned into the MCS of an GreS base vector ...
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bioRxiv - Molecular Biology 2024Quote: ... and the human herpes simplex virus 5 puromycin resistance marker (Clontech).
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bioRxiv - Microbiology 2022Quote: ... the CPER products were transfected into HEK293-C34 cells using TransIT-LT1 (Takara, Cat# MIR2300) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... the CPER products were transfected into HEK293-C34 cells using TransIT-LT1 (Takara, Cat# MIR2300) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... the CPER products were transfected into HEK293-C34 cells using TransIT-LT1 (Takara, cat# MIR2300) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2019Quote: ... HEK293T and CHO-K1 cell lines were ordered from American Type Culture Collection (ATCC) and HEK293-tetON cells and CHO-tetON cells were purchased form Clontech (Takara Bio). Synthetic DNA was ordered from Twist Bioscience.
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bioRxiv - Cell Biology 2022Quote: ... Lentiviruses containing guide RNAs targeting GORAB were created in HEK293 Lenti-X cells (Takara 31966021) plated on 10 cm dishes the day before transfection ...
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bioRxiv - Genomics 2019Quote: ... We concentrated the virus 100-fold with Lenti-X concentrator (Clontech, 631231) following the manufacturer’s recommendations before aliquoting and freezing at -80C.
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bioRxiv - Cancer Biology 2023Quote: ... Virus particles were precipitated with Lenti-X Concentrator (#631232, Takara Bio, Japan), suspended in phosphate buffer saline (PBS ...
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bioRxiv - Immunology 2023Quote: ... Virus supernatant was harvested three days later and coated on retronectin (TaKaRa)-treated plates (incubated overnight at 4°C previously ...
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bioRxiv - Cell Biology 2024Quote: ... Virus was produced by Lenti-X™ 293T cells (Takara Bio USA), transfected with lentiviral expression vector (330 ng) ...
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bioRxiv - Microbiology 2023Quote: ... we further concentrated virus stocks using Lenti-X™ Concentrator (Takara, 631232) to between 3.5*10^5-1.5*10^6 TU/ml.
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bioRxiv - Bioengineering 2023Quote: HEK293 cells (JRCB Cell Bank, Osaka, Japan) and AAVpro 293T cells (Takara Bio Inc., Shiga, Japan) were cultured in Dulbecco’s modified Eagle Medium (Sigma Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... The virus-containing media were harvested and concentrated with Lenti-X concentrator (Clontech) per the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... Virus-containing supernatants were concentrated with Lenti-X and Retro-X concentrator (Takara), respectively ...
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bioRxiv - Cell Biology 2019Quote: ... after which media was collected and virus was concentrated with Lenti-X (Clontech) according to manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Virus-rich supernatants were concentrated using Lenti-X™ Concentrator (Takara Bio, 631231) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2024Quote: ... The virus titer was measured using Adeno-X Rapid Titer Kit (Takara Bio) and 108 pfu (plaque-forming units ...
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bioRxiv - Microbiology 2023Quote: ... the CPER products were transfected into HEK293-hACE2/hTMPRSS2 cells by using TransIT-LT1 (Takara, Cat# MIR2305). At one day post-transfection ...
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bioRxiv - Neuroscience 2020Quote: ... and the virus collected after 72h using Lenti-X-concentrator (Cat. No. 631231, Clontech).
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bioRxiv - Bioengineering 2021Quote: ... Virus-containing supernatants were concentrated with Lenti-X Concentrator or Retro-X concentrator (Takara), respectively ...
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bioRxiv - Cell Biology 2022Quote: ... The titer of the virus was determined by Adeno-X Rapid Titer Kit (Clontech). Cells were infected with 50 MOI for 48 hr ...
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bioRxiv - Cell Biology 2020Quote: ... culture supernatants were collected and virus concentrated 10x overnight with Lenti-X concentrator (Clontech) according to the manufacturer ...
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bioRxiv - Cancer Biology 2023Quote: ... virus-containing supernatants were first concentrated 10x using Lenti-X Concentrator (Takara, Cat# 631232). Single cell-suspended organoids were mixed with concentrated virus ...
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bioRxiv - Neuroscience 2023Quote: ... and then the virus solution was concentrated using the Lenti-X Concentrator (631231, Clontech) and suspended in PBS (in vivo conditions).
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bioRxiv - Cancer Biology 2023Quote: ... Virus was titered using the Lenti-X p24 Rapid Titer Kit (Takara Cat #632200).
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bioRxiv - Physiology 2024Quote: ... Virus containing medium was collected at 48h and concentrated using Lenti-XTM Concentrator (Takara). C2C12 mouse myoblasts were seeded 24h prior to infection and then transduced with virus-containing supernatant supplemented with 8μg/mL polybrene (Millipore ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 cells were transfected with pX330 vector together with an EGFP-containing plasmid (pEGFP-N1; Clontech Laboratories, Inc). Single cells were sorted using a FACS Vantage SE machine and the knockouts confirmed by sequencing ...
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bioRxiv - Neuroscience 2021Quote: ... The transfection efficiency of HEK293 cells was evaluated by transfecting cells with EGFP-N1 (Clontech; Mountain View, CA) in parallel reactions ...
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bioRxiv - Neuroscience 2022Quote: All viruses were made using the 2nd generation lentiviral packaging systems in Lenti-X HEK293 FTT cells (Takara). Lenti-X cells were passaged maximum 3 times before being used for virus production in HEK media (High glucose DMEM with 4 mM GlutaMAX ...
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bioRxiv - Cancer Biology 2021Quote: ... Virus transduction was performed using RetroNectin® Recombinant Human Fibronectin Fragment (Takara, cat.no. T100A/B) according to the manufacturer’s instructions (with centrifugation) ...
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bioRxiv - Cancer Biology 2022Quote: ... 10 ml of virus containing media were concentrated with Lenti-X concentrator (Takara Bio, 631231) to a titer sufficient to decrease pStat1 levels by 25% (Mean pStat1+ CD11b+ cells ...
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bioRxiv - Microbiology 2022Quote: ... cDNA was produced by using Moloney murine leukemia virus (Mo-MLV) reverse transcriptase (TaKaRa, Japan) with a reverse primer ...
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bioRxiv - Cancer Biology 2019Quote: ... Virus was collected after 48 hours and concentrated with Lenti-X Concentrator (Clontech, Cat#631231) (16) ...
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bioRxiv - Microbiology 2022Quote: ... The purified virus titer was determined using an Adeno-X Rapid Titer kit (Takara Bio). Sex and age at sampling for the cells were as follows ...
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bioRxiv - Cancer Biology 2023Quote: ... The virus titer was determined with Lenti-X™ p24 Rapid Titration ELISA Kit (Takara), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... The virus titer was measured using Lenti-X GoStix Plus assay kit (Takara Bio, 631280).