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Citations for Takara Bio :
51 - 100 of 526 citations for Mayaro Virus E2 Envelope Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... Foamy virus was produced using Lenti-X 293T (Takara Bio, Kusatsu) generated as previously described and frozen at -80°C for long term storage42 ...
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bioRxiv - Neuroscience 2023Quote: Rabies genomes were exacted by NucleoSpin® RNA Virus (740956.50, Takara), RT-PCR was performed with AccuScript PfuUltra II RT-PCR Kit (600184 ...
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bioRxiv - Physiology 2024Quote: ... Virus concentration was determined using Lenti-X GoStix Plus (Takara Bio) after one freeze-thaw cycle to be consistent with experimental conditions.
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bioRxiv - Molecular Biology 2024Quote: ... separated by an encephalomyelitis virus internal ribosome entry site (IRES) (Clontech), were cloned into the MCS of an GreS base vector ...
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bioRxiv - Molecular Biology 2024Quote: ... and the human herpes simplex virus 5 puromycin resistance marker (Clontech).
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Potential for virus endogenization in humans through testicular germ cell infection: the case of HIVbioRxiv - Microbiology 2020Quote: ... or pBR_NL4-3 92TH014.12 IRES eGFP (R5 tropic) [81] (a kind gift from Frank Kirchhoff, Ulm University, Germany) and VSV-G envelope encoding plasmid (PT3343-5, Clontech). 293T cell supernatants were collected 3 days post-transfection ...
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Potential for virus endogenization in humans through testicular germ cell infection: the case of HIVbioRxiv - Microbiology 2020Quote: ... VSV G-pseudotyped HIV-1 using full-length HIV-1 molecular clone pNL4-3 [79] or the R5-tropic pNL4-3 AD8 derivative [80] and VSV-G envelope encoding plasmid (PT3343-5, Clontech); 4 ...
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bioRxiv - Cancer Biology 2022Quote: ... in which pRetroX-TetOne puro empty vector or pRetroX-TetOne puro-ALKBH5 were each cotransfected with the envelope vector VSV-G using Xfect transfection reagent (Clontech). BEAS2B ...
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bioRxiv - Immunology 2022Quote: ... 25 μg of the retroviral expression vector (pLZRS-human codon-optimized Bcl6-P2A-human codon-optimized BclxL-IRES-GFP) and 5 μg of the envelope vector (p10A1; Clontech) were transfected into GP2-293 cells using Lipofectamine 3000 (ThermoFisher ...
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bioRxiv - Immunology 2021Quote: ... The retroviral transfer vector was co-transfected with pVSV-G retroviral envelope-expressing plasmid into the GP2-293 cell line (Clontech-TaKaRa) using Lipofectamine 2000 reagent (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... Lentiviral particles were produced by co-transfecting the barcoding plasmid with the packaging and envelope plasmids pMD2G and pCMV-dR8.91 (provided by Andrew Ramos) into Lenti-X 293T cells (Takara, 632180) with Lipofectamine 2000 (Thermo Fisher ...
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bioRxiv - Immunology 2023Quote: Lentivirus was generated by co-transfecting the barcoding plasmid with the packaging and envelope plasmids pMD2G and pCMV-dR8.91 (provided by Andrew Ramos) into Lenti-X 293T cells (Takara, 632180) using Lipofectamine 2000 (Thermo Fisher ...
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bioRxiv - Genomics 2019Quote: ... We concentrated the virus 100-fold with Lenti-X concentrator (Clontech, 631231) following the manufacturer’s recommendations before aliquoting and freezing at -80C.
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bioRxiv - Cancer Biology 2023Quote: ... Virus particles were precipitated with Lenti-X Concentrator (#631232, Takara Bio, Japan), suspended in phosphate buffer saline (PBS ...
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bioRxiv - Immunology 2023Quote: ... Virus supernatant was harvested three days later and coated on retronectin (TaKaRa)-treated plates (incubated overnight at 4°C previously ...
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bioRxiv - Cell Biology 2024Quote: ... Virus was produced by Lenti-X™ 293T cells (Takara Bio USA), transfected with lentiviral expression vector (330 ng) ...
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bioRxiv - Microbiology 2023Quote: ... we further concentrated virus stocks using Lenti-X™ Concentrator (Takara, 631232) to between 3.5*10^5-1.5*10^6 TU/ml.
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bioRxiv - Systems Biology 2022Quote: ... Plasmids containing mKO2-hCdt1 (30-120) and mAG-hGeminin (1-110) were co-transfected with envelope and packaging plasmids into LentiX-293T cells (Takara bio) to generate lentiviral particles ...
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bioRxiv - Immunology 2021Quote: ... The retroviral transfer vector was co-transfected with pVSV-G retroviral envelope-expressing plasmid into the GP2-293 cell line (Clontech-TaKaRa) using Lipofectamine 2000 reagent (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... packaging (pCAG-HIVgp) and envelope (pCMV-VSV-G-RSV-Rev) plasmids were co-transfected into Lenti-X 293T cells (Takara Bio) using Fugene 6 reagent (Promega) ...
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bioRxiv - Cell Biology 2022Quote: ... The virus-containing media were harvested and concentrated with Lenti-X concentrator (Clontech) per the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... Virus-containing supernatants were concentrated with Lenti-X and Retro-X concentrator (Takara), respectively ...
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bioRxiv - Cell Biology 2019Quote: ... after which media was collected and virus was concentrated with Lenti-X (Clontech) according to manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Virus-rich supernatants were concentrated using Lenti-X™ Concentrator (Takara Bio, 631231) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2024Quote: ... The virus titer was measured using Adeno-X Rapid Titer Kit (Takara Bio) and 108 pfu (plaque-forming units ...
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bioRxiv - Neuroscience 2020Quote: ... and the virus collected after 72h using Lenti-X-concentrator (Cat. No. 631231, Clontech).
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bioRxiv - Bioengineering 2021Quote: ... Virus-containing supernatants were concentrated with Lenti-X Concentrator or Retro-X concentrator (Takara), respectively ...
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bioRxiv - Cell Biology 2022Quote: ... The titer of the virus was determined by Adeno-X Rapid Titer Kit (Clontech). Cells were infected with 50 MOI for 48 hr ...
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bioRxiv - Cell Biology 2020Quote: ... culture supernatants were collected and virus concentrated 10x overnight with Lenti-X concentrator (Clontech) according to the manufacturer ...
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bioRxiv - Cell Biology 2022Quote: ... Virus containing supernatants were harvested five times from transfected Ecopack-HEK293 cells (Clontech/Takara), over a period of three days post-transfection and filtered with 0.45 µm filters ...
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bioRxiv - Cell Biology 2022Quote: ... Virus containing supernatants were harvested five times from transfected Ecopack-HEK293 cells (Clontech/Takara), over a period of three days post-transfection and filtered with 0.45 µm filters ...
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bioRxiv - Cancer Biology 2023Quote: ... virus-containing supernatants were first concentrated 10x using Lenti-X Concentrator (Takara, Cat# 631232). Single cell-suspended organoids were mixed with concentrated virus ...
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bioRxiv - Neuroscience 2023Quote: ... and then the virus solution was concentrated using the Lenti-X Concentrator (631231, Clontech) and suspended in PBS (in vivo conditions).
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bioRxiv - Cancer Biology 2023Quote: ... Virus was titered using the Lenti-X p24 Rapid Titer Kit (Takara Cat #632200).
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bioRxiv - Physiology 2024Quote: ... Virus containing medium was collected at 48h and concentrated using Lenti-XTM Concentrator (Takara). C2C12 mouse myoblasts were seeded 24h prior to infection and then transduced with virus-containing supernatant supplemented with 8μg/mL polybrene (Millipore ...
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bioRxiv - Cancer Biology 2021Quote: ... Virus transduction was performed using RetroNectin® Recombinant Human Fibronectin Fragment (Takara, cat.no. T100A/B) according to the manufacturer’s instructions (with centrifugation) ...
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bioRxiv - Cancer Biology 2022Quote: ... 10 ml of virus containing media were concentrated with Lenti-X concentrator (Takara Bio, 631231) to a titer sufficient to decrease pStat1 levels by 25% (Mean pStat1+ CD11b+ cells ...
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bioRxiv - Microbiology 2022Quote: ... cDNA was produced by using Moloney murine leukemia virus (Mo-MLV) reverse transcriptase (TaKaRa, Japan) with a reverse primer ...
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bioRxiv - Cancer Biology 2019Quote: ... Virus was collected after 48 hours and concentrated with Lenti-X Concentrator (Clontech, Cat#631231) (16) ...
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bioRxiv - Microbiology 2022Quote: ... The purified virus titer was determined using an Adeno-X Rapid Titer kit (Takara Bio). Sex and age at sampling for the cells were as follows ...
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bioRxiv - Cancer Biology 2023Quote: ... The virus titer was determined with Lenti-X™ p24 Rapid Titration ELISA Kit (Takara), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... The virus titer was measured using Lenti-X GoStix Plus assay kit (Takara Bio, 631280).
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bioRxiv - Cancer Biology 2023Quote: ... Lenti-X GoStix Plus were then used to determine virus titer (Takara Bio cat#631280). For lentiviral infection ...
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bioRxiv - Immunology 2024Quote: ... The virus particles were then purified using the Aneno-X Maxi Purification Kit (Takara Bio), and the buffer was replaced with 1X Formulation Buffer containing 2.5% glycerol (w/v) ...
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bioRxiv - Microbiology 2024Quote: ... A3 encapsidation was determined by concentrating the virus containing supernatant using Retro-X Concentrator (Clontech) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... Virus was plated on non-tissue culture treated 24-well plates precoated with retronectin (TaKaRa) overnight at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... Virus titer was measured by using Lenti-X p24 Rapid Titer Kit (Cat #022261, Takara).
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bioRxiv - Cancer Biology 2021Quote: ... Virus was collected at 48 hours and 72 hours and concentrated with Lenti-X Concentrator (Clontech). The Virus was resuspended in DMEM and stored at -80C.
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bioRxiv - Cell Biology 2019Quote: ... after which virus-containing supernatants were harvested and concentrated ∼40-fold using Lenti-X Concentrator (Clontech) per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The virus was then quantified using the Lenti-X™ p24 Rapid Titer Kit (Takara Bio) and aliquots were frozen at −80° C for future use.