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Citations for Takara Bio :
1 - 50 of 68 citations for Ion Exchange Chromatography since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... in Terrific Broth and purified by metal ion affinity chromatography (IMAC) on TALON/Cobalt beads (Clontech) using 1M NaCl containing buffers to prevent the potential co-purification of E ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant protein was then purified by immobilized metal ion affinity chromatography using a cobalt-based matrix (Talon, Clontech) and eluted with 100 mM imidazole ...
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bioRxiv - Biochemistry 2021Quote: ... Recombinant protein was then purified by immobilized metal ion affinity chromatography using a cobalt-based matrix (Talon, Clontech) and eluted with 100 mM imidazole ...
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant containing Rap1 was further purified by Immobilized Metal ion Affinity Chromatography using TALON Metal Affinity Resin (Clontech) containing Co2+ cations as described (Yanez et al ...
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bioRxiv - Biophysics 2021Quote: ... The resulting supernatant was collected by ultracentrifugation (150,000 × g, 1 hour, 4 °C) and applied onto an immobilized metal-ion affinity chromatography column (Talon, Clontech) equilibrated with 40 mM Tris ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant protein were purified to >90% electrophoretic purity by immobilized metal ion affinity chromatography using a cobalt-based matrix (Talon, Clontech) and eluted with imidazole as described previously24 ...
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bioRxiv - Biochemistry 2024Quote: ... pLysS or Rosetta pLysS cells and overexpressed proteins were purified using immobilized metal-ion affinity chromatography (IMAC) with TALON resin (Clontech) (Abad et al ...
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bioRxiv - Biochemistry 2021Quote: ... Cfr was purified by Talon chromatography (Clontech) from clarified lysates ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Fabs were purified by cobalt chromatography (Takara) and further purified over an S200 column on an AKTA pure (Cytiva) ...
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bioRxiv - Biochemistry 2019Quote: ... followed by affinity chromatography using Co Talon resin (Clontech). Further purification was accomplished by ion exchange and size-exclusion chromatography on an AKTA PURE system (GE Healthcare).
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bioRxiv - Bioengineering 2020Quote: ... and purified using Co2+ metal affinity chromatography (TALON, Clontech) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... IC3 was purified by affinity chromatography (TALON resin, Clontech) using the two N-terminal histidine tags of aIF5B and aIF1A ...
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bioRxiv - Cell Biology 2020Quote: ... cerevisiae Tip20 was purified using TALON affinity chromatography (Clontech) in 20 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2019Quote: ... The resulting enzyme was then purified by Talon chromatography (Clontech) and underwent iron-sulfur cluster reconstitution using previously published protocols 26,30,31.
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bioRxiv - Biochemistry 2023Quote: ... The protein was purified by metal-affinity chromatography (Talon resin, Clontech), the affinity tag removed using tobacco etch virus (TEV ...
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bioRxiv - Cancer Biology 2022Quote: ... CDC37 was expressed as previously described using Talon metal affinity chromatography (Takara Bio company ...
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bioRxiv - Biochemistry 2023Quote: ... affinity chromatography (Dsl1:Qb:Qc and His7-Tip20:Sec20) or TALON (Takara Bio) affinity chromatography (all other complexes).
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bioRxiv - Immunology 2023Quote: ... Recombinant cholix toxin was first purified on nickel metal affinity chromatography (Takara) and subsequent TEV protease-mediated 6His-MBP tag removal at 4°C/Over Night (ON) ...
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bioRxiv - Genetics 2023Quote: ... 200186 and purified by cobalt metal-affinity chromatography (Talon resin, Clontech 635652). cFos/cJun was stored in 18 mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2024Quote: ... the LHA and RHA PCR products were used as primers for amplifying the tsp exchange gene using CloneAmp HiFi PCR Premix (Takara Bio). After five PCR cycles ...
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bioRxiv - Molecular Biology 2019Quote: ... LDLR-ECD was enriched by affinity chromatography using TALON superflow affinity resin (Clontech) followed by gel filtration chromatography on a Superdex 200 10/300 GL column (GE Healthcare).
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bioRxiv - Biochemistry 2021Quote: ... Proteins in the supernatant were purified by the affinity chromatography with TALON (Clontech) resin ...
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bioRxiv - Genomics 2024Quote: ... followed by an Amicon filter (MilliporeSigma, Burlington, MA) buffer exchange (Vaillancourt et al., 2019) or Takara NucleoBond HMW DNA kit (Takara, Kusatsu, Shiga, Japan). Genome assembly used hifiasm v0.16.1-r375 (Cheng et al ...
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bioRxiv - Cell Biology 2020Quote: ... HIS-Ub-conjugated proteins were purified by cobalt chromatography (TALON metal affinity resin, Clontech), as described in the manual ...
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bioRxiv - Immunology 2021Quote: ... and the supernatant was subjected to affinity chromatography using Ni-NTA beads column (Takara). Eluted protein was dialyzed against 1×PBS with four changes for two days at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... target proteins were purified from cell lysate by Ni-iminodiacetic acid affinity chromatography (Clontech). However ...
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bioRxiv - Cell Biology 2020Quote: ... The proteins were first purified by affinity chromatography using TALON metal affinity resin (TaKaRa), and further purified via gel filtration chromatography on a Superdex 200 column (GE Healthcare ...
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bioRxiv - Biophysics 2022Quote: ... and ACE2 was purified by nickel affinity chromatography using His60 Ni Superflow Resin (Takara) followed by size exclusion chromatography on a Superdex 200 column (Cytiva ...
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bioRxiv - Immunology 2023Quote: ... and supernatants were purified via immobilized metal affinity chromatography using Cobalt-TALON resin (Takara). Eluted proteins were further purified by size-exclusion chromatography using a Superdex 200 Increase 10/300 GL column (Cytiva ...
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bioRxiv - Biophysics 2024Quote: ... The purification process involved an affinity chromatography step using cobalt affinity resin (TALON, Clontech), followed by a size exclusion chromatography (SEC ...
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bioRxiv - Cancer Biology 2023Quote: ... Centrifuged lysate was purified by gravity-flow chromatography on TALON Metal Affinity Resin (Clontech) following the manufacturer’s protocol with 0.05% Triton X-100 added to all buffers ...
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bioRxiv - Biophysics 2024Quote: ... MelBSt purification from membranes by cobalt-affinity chromatography (Talon Superflow Metal Affinity Resin, Takara) after extraction by 1.5% UDM ...
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bioRxiv - Microbiology 2020Quote: ... The Spike ectodomain was purified by immobilised metal affinity chromatography using Talon resin (Takara Bio) charged with cobalt followed by size exclusion chromatography using HiLoad 16/60 Superdex 200 column in 150 mM NaCl ...
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bioRxiv - Microbiology 2020Quote: ... The Spike ectodomain was purified by immobilized metal affinity chromatography using Talon resin (Takara Bio) charged with cobalt followed by size exclusion chromatography using HiLoad 16/60 Superdex 200 column in 150 mM NaCl ...
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bioRxiv - Plant Biology 2022Quote: ... Proteins were purified by immobilized-metal affinity chromatography using TALON® metal affinity resin (Clontech), where filtered supernatant (0.45 μm filter ...
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bioRxiv - Neuroscience 2019Quote: ... following 0.5 mM IPTG induction for 17h at 28 °C and purification by TALON chromatography (Clontech), essentially as described10 ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatants were applied to a chromatography column packed with 5 ml His60 superflow resin (Clontech) that had been equilibrated with buffer A (20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was subjected to immobilized metal affinity chromatography (IMAC) with talon resin (Takara Bio USA). The bound protein was washed with TBS buffer containing 0.02% GDN and 20 mM imidazole and eluted with TBS buffer containing 0.02% GDN and 250 mM imidazole ...
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bioRxiv - Genetics 2022Quote: ... Small DNA fragments were removed by spin-column chromatography with Chroma-Spin-1000+TE columns (Clontech) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... aIF1A was purified as described (24) except that a standard affinity chromatography step on Talon resin (Clontech) was added to the original protocol ...
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bioRxiv - Biochemistry 2020Quote: ... its variant PmCpeB_C82A and CpcB were purified by affinity chromatography using TALON® metal affinity resin (Clontech) and purification was carried out according to the manufacturer’s instructions based on sodium phosphate buffer (60 mM ...
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bioRxiv - Cell Biology 2020Quote: ... coli cells and purified to near-homogeneity by metal-affinity chromatography using TALON resin (Takara Bio, USA). After purification ...
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bioRxiv - Immunology 2021Quote: ... Supernatant from Expi293 transfected cells was manually purified using TALON metal affinity chromatography (Takara bio Inc – 635502), according to manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was incubated overnight with 0.5 ml of Talon (immobilised metal affinity chromatography, IMAC) resin (Clontech) in the presence of 150 mM NaCl and 20 mM imidazole ...
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bioRxiv - Genetics 2019Quote: ... following 0.5 mM IPTG induction for 4 h at 25°C with subsequent purification using TALON chromatography (Clontech) as described 34 ...
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bioRxiv - Microbiology 2022Quote: ... CydDC was purified via His6-tag affinity chromatography using a Talon (Co-IMAC) affinity matrix (Takara Bio Inc.). For column conditioning and washing step I (10 column volumes [CV] ...
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bioRxiv - Microbiology 2023Quote: ... The His-tagged CRONE protein was purified by cobalt affinity chromatography using TALON® metal affinity resin (Takara) under native conditions in phosphate buffered saline (PBS) ...
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bioRxiv - Immunology 2021Quote: ... supernatants were harvested by centrifugation and further purified using immobilized metal affinity chromatography (IMAC) with cobalt-TALON resin (Takara) followed by Superdex 200 Increase 10/300 GL size exclusion column (GE Healthcare).
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bioRxiv - Microbiology 2019Quote: ... The Pfc43opt recombinant protein was soluble and purified by cobalt affinity chromatography using TALON® metal affinity resin (Takara) under native conditions in phosphate buffered saline (PBS) ...
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bioRxiv - Immunology 2023Quote: ... S-IgA was purified by two-step chromatography using the Capturem His-Taged Purification Kit (Takara Bio, Shiga, Japan) followed by size exclusion chromatography (Cytiva ...