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Citations for Takara Bio :
51 - 100 of 256 citations for Influenza Virus Hemagglutinin HA H9N2 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Primary antibodies anti-HA.11 (Clontech, 631207) or anti-A3H were used at a dilution at 1:500 and 1:50 ...
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bioRxiv - Systems Biology 2020Quote: ... or pCMV-HA-N vector (Clontech #635690), respectively ...
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bioRxiv - Cell Biology 2019Quote: ... and subcloned into pCMV-HA vector (Clontech) at the SalI/XhoI sites ...
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bioRxiv - Plant Biology 2020Quote: ... Using the cDNA synthesis kit (TaKaRa: Moloney Murine Leukemia Virus Version), cDNA was synthesized from extracted RNA ...
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bioRxiv - Cancer Biology 2020Quote: ... The titer of virus was measured using P24 ELISA kit (Clontech).The virus was aliquoted and stored at −80°C.
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bioRxiv - Molecular Biology 2020Quote: ... virus supernatant was concentrated with Lenti-X™ Concentrator (Takara, #631231) as per the manufacturer’s protocol.
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bioRxiv - Biochemistry 2020Quote: ... Virus titer was determined using Lenti-XTM GoStixTM Plus (Takara Bio), and Huh7 cells were infected at a MOI of 5 ...
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bioRxiv - Genetics 2022Quote: ... Virus titer was estimated using Lenti-X GoStix Plus (Takara, 631281) after 100x dilution ...
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bioRxiv - Immunology 2023Quote: ... The virus titer was determined using Lenti-XTM GoStix Plus (Clontech).
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bioRxiv - Cell Biology 2024Quote: ... The virus titer was determined using Lenti-X GoStix Plus (Clontech). To transduce hTCEpi cells ...
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bioRxiv - Developmental Biology 2024Quote: ... Foamy virus was produced using Lenti-X 293T (Takara Bio, Kusatsu) generated as previously described and frozen at -80°C for long term storage42 ...
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bioRxiv - Neuroscience 2023Quote: Rabies genomes were exacted by NucleoSpin® RNA Virus (740956.50, Takara), RT-PCR was performed with AccuScript PfuUltra II RT-PCR Kit (600184 ...
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bioRxiv - Physiology 2024Quote: ... Virus concentration was determined using Lenti-X GoStix Plus (Takara Bio) after one freeze-thaw cycle to be consistent with experimental conditions.
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bioRxiv - Molecular Biology 2024Quote: ... separated by an encephalomyelitis virus internal ribosome entry site (IRES) (Clontech), were cloned into the MCS of an GreS base vector ...
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bioRxiv - Molecular Biology 2024Quote: ... and the human herpes simplex virus 5 puromycin resistance marker (Clontech).
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bioRxiv - Physiology 2024Quote: ... the full-length HA-TTL was amplified from the HA-TTL-IRES-dsRed plasmid using PrimeStar GLX Polymerase (Takara Clontech) and ligated into the NheI and BamHI restriction sites of VC206 ...
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bioRxiv - Physiology 2024Quote: ... the full-length HA-TTL was amplified from the HA-TTL-IRES-dsRed plasmid using PrimeStar GLX Polymerase (Takara Clontech) and ligated into the NheI and BamHI restriction sites of VC206 ...
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bioRxiv - Biochemistry 2020Quote: ... HA-tag polyclonal Antibody (631207) was from Clontech.
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bioRxiv - Bioengineering 2023Quote: ... HA-mEPOR was retrovirally transduced with pMSCVneo (Clontech) and selected with 0.6 U/ml human erythropoietin (Epoetin Alfa ...
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bioRxiv - Microbiology 2023Quote: ... the cells were transfected with the eukaryotic expression plasmid harbouring USP8 (pCMV-HA-USP) or Empty vector (pCMV-HA) using X-fect (TAKARA Bio) transfection reagent according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... were sub-cloned from pCMV-HA-BTN3A2-L and pCMV-HA-BTN3A2-S expression vectors into the pLVX-tight-puro vector (632162, Clontech, USA) (Supplementary Table S1) ...
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bioRxiv - Genomics 2019Quote: ... We concentrated the virus 100-fold with Lenti-X concentrator (Clontech, 631231) following the manufacturer’s recommendations before aliquoting and freezing at -80C.
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bioRxiv - Cancer Biology 2023Quote: ... Virus particles were precipitated with Lenti-X Concentrator (#631232, Takara Bio, Japan), suspended in phosphate buffer saline (PBS ...
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bioRxiv - Immunology 2023Quote: ... Virus supernatant was harvested three days later and coated on retronectin (TaKaRa)-treated plates (incubated overnight at 4°C previously ...
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bioRxiv - Cell Biology 2024Quote: ... Virus was produced by Lenti-X™ 293T cells (Takara Bio USA), transfected with lentiviral expression vector (330 ng) ...
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bioRxiv - Microbiology 2023Quote: ... we further concentrated virus stocks using Lenti-X™ Concentrator (Takara, 631232) to between 3.5*10^5-1.5*10^6 TU/ml.
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bioRxiv - Immunology 2019Quote: IFITM constructs cloned into the pCMV-HA vector (Clontech) were described previously (13 ...
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bioRxiv - Cell Biology 2020Quote: ... Vps4A E228Q was cloned into the pCMV-HA (Clontech) vector using restriction enzymes EcoRI and NotI ...
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bioRxiv - Cell Biology 2022Quote: ... The virus-containing media were harvested and concentrated with Lenti-X concentrator (Clontech) per the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... Virus-containing supernatants were concentrated with Lenti-X and Retro-X concentrator (Takara), respectively ...
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bioRxiv - Cell Biology 2019Quote: ... after which media was collected and virus was concentrated with Lenti-X (Clontech) according to manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Virus-rich supernatants were concentrated using Lenti-X™ Concentrator (Takara Bio, 631231) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2024Quote: ... The virus titer was measured using Adeno-X Rapid Titer Kit (Takara Bio) and 108 pfu (plaque-forming units ...
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bioRxiv - Cell Biology 2022Quote: ... Sar1[T39N]-HA in pTARGET or in IRES-GFP (Clontech) were used before 6 ...
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bioRxiv - Neuroscience 2020Quote: ... and the virus collected after 72h using Lenti-X-concentrator (Cat. No. 631231, Clontech).
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bioRxiv - Bioengineering 2021Quote: ... Virus-containing supernatants were concentrated with Lenti-X Concentrator or Retro-X concentrator (Takara), respectively ...
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bioRxiv - Cell Biology 2022Quote: ... The titer of the virus was determined by Adeno-X Rapid Titer Kit (Clontech). Cells were infected with 50 MOI for 48 hr ...
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bioRxiv - Cell Biology 2020Quote: ... culture supernatants were collected and virus concentrated 10x overnight with Lenti-X concentrator (Clontech) according to the manufacturer ...
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bioRxiv - Cell Biology 2022Quote: ... Virus containing supernatants were harvested five times from transfected Ecopack-HEK293 cells (Clontech/Takara), over a period of three days post-transfection and filtered with 0.45 µm filters ...
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bioRxiv - Cell Biology 2022Quote: ... Virus containing supernatants were harvested five times from transfected Ecopack-HEK293 cells (Clontech/Takara), over a period of three days post-transfection and filtered with 0.45 µm filters ...
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bioRxiv - Cancer Biology 2023Quote: ... virus-containing supernatants were first concentrated 10x using Lenti-X Concentrator (Takara, Cat# 631232). Single cell-suspended organoids were mixed with concentrated virus ...
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bioRxiv - Neuroscience 2023Quote: ... and then the virus solution was concentrated using the Lenti-X Concentrator (631231, Clontech) and suspended in PBS (in vivo conditions).
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bioRxiv - Cancer Biology 2023Quote: ... Virus was titered using the Lenti-X p24 Rapid Titer Kit (Takara Cat #632200).
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bioRxiv - Physiology 2024Quote: ... Virus containing medium was collected at 48h and concentrated using Lenti-XTM Concentrator (Takara). C2C12 mouse myoblasts were seeded 24h prior to infection and then transduced with virus-containing supernatant supplemented with 8μg/mL polybrene (Millipore ...
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bioRxiv - Microbiology 2023Quote: ... HA trimers were purified by passage over Co-NTA agarose (Clontech) followed by gel filtration chromatography on Superdex 200 (GE Healthcare ...
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bioRxiv - Microbiology 2023Quote: ... HA was purified by passage over TALON Metal Affinity Resin (Takara) followed by gel filtration chromatography on Superdex 200 (GE Healthcare ...
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bioRxiv - Cancer Biology 2021Quote: ... Virus transduction was performed using RetroNectin® Recombinant Human Fibronectin Fragment (Takara, cat.no. T100A/B) according to the manufacturer’s instructions (with centrifugation) ...
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bioRxiv - Cancer Biology 2022Quote: ... 10 ml of virus containing media were concentrated with Lenti-X concentrator (Takara Bio, 631231) to a titer sufficient to decrease pStat1 levels by 25% (Mean pStat1+ CD11b+ cells ...
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bioRxiv - Microbiology 2022Quote: ... cDNA was produced by using Moloney murine leukemia virus (Mo-MLV) reverse transcriptase (TaKaRa, Japan) with a reverse primer ...
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bioRxiv - Cancer Biology 2019Quote: ... Virus was collected after 48 hours and concentrated with Lenti-X Concentrator (Clontech, Cat#631231) (16) ...