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Citations for Takara Bio :
551 - 600 of 878 citations for IFN γ Receptor II Human CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: PtK2 GFP-α-tubulin cells (stable line expressing human α-tubulin in pEGFP-C1; Takara Bio Inc. ...
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bioRxiv - Immunology 2021Quote: ... and cDNA encoding human full-length MuSK was cloned into pIRES2-EGFP plasmid vector (Clontech). AChR and MuSK vectors were kindly provided by Drs ...
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bioRxiv - Neuroscience 2022Quote: 50 µg of human cerebral cortex lysate (Protein Medley, Takara, Kusatsu, Japan, Cat. No. 635323) were diluted and prepared according to manufacturer’s instructions (protocol PT1602-1) ...
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bioRxiv - Molecular Biology 2022Quote: ... The bait stain was combined with a Mate & Plate™ Human Heart library (Takara Bio) for 24 hours after which cells were plated on selective synthetic defined (SD ...
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bioRxiv - Neuroscience 2022Quote: ... and either 4 wells of 10 pg of Human Universal Reference Total RNA (Takara 636538) or 2 wells of 10 pg of Human Universal Reference and 2 wells of 10 pg Control RNA provided in the Clontech kit ...
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bioRxiv - Cell Biology 2023Quote: ... Aurora A and TPX2 were amplified from human testis cDNA (Marathon cDNA, Takara Bio Inc.) using Pfu polymerase (Promega) ...
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bioRxiv - Cell Biology 2023Quote: ... and SOX4 were amplified from a reverse transcript of Human Fetal Brain Total RNA (Takara) by high-fidelity PCR using Phusion DNA Polymerases (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... mRNA was utilized with the SMARTer Human TCR a/b Profiling Kit v2 (Takara, USA). The resulting libraries were sequenced for paired-end reads of 2×250 bp on an Illumina system at a sequencing depth of 7.5X.
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bioRxiv - Developmental Biology 2023Quote: ... The ORF of human KIF23 was cloned within pCAX vector using In-Fusion system (TaKaRa). The disease associated c.755T>A mutation was introduced to human KIF23 cDNA by the PCR-based mutagenesis as described (Xia et al. ...
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bioRxiv - Cell Biology 2020Quote: ... and reverse transcribed into cDNA using reagents from a Prime Script II first strand cDNA synthesis kit (Takara). Real-time PCR was performed with the reverse transcription products ...
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bioRxiv - Microbiology 2021Quote: ... Viral RNA was amplified using PrimeScript II High Fidelity One Step RT-PCR Kit (Takara Bio, Shiga, Japan) under the following reaction conditions ...
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bioRxiv - Developmental Biology 2020Quote: Total RNA was extracted from cells using the NucleoSpin RNA II kit (Clontech Laboratories, Mountain View, CA, USA). The quality and quantity of total RNA were measured by Nanodrop 1000 (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: ... Quantitative Real Time PCR reactions were performed with the TB Green™ Premix Ex Taq™ II (TAKARA) as recommended by the supplier ...
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bioRxiv - Molecular Biology 2019Quote: ... cDNA was amplified using the primer sets listed in Table S5 with SYBR Premix Ex Taq II (Takara) in accordance with the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Quantitative real-time PCR analysis was performed using the SYBR Premix Ex TaqTM II kit (Takara, Dalian, China). HMGB1 primers were designed using Primier4.1 software and GADPH was used as a reference gene ...
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bioRxiv - Physiology 2021Quote: Real-time qRT–PCR was performed on cDNA amplified by PrimeScript II 1st Strand cDNA Synthesis Kit (Takara) using GoTaq qPCR (Promega ...
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bioRxiv - Plant Biology 2020Quote: ... and reverse-transcribed with a random hexamer or oligo(dT)24 primer using SYBR Premix ExTaq II (TaKaRa). qRT-PCR reactions were performed with gene-specific forward and reverse primers using the PrimeScript RT-PCR kit (TaKaRa ...
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bioRxiv - Microbiology 2020Quote: ... The qRT-PCR was performed using the TB Green Premix Ex Taq II (Takara Bio Inc., Shiga, Japan) on a QuantStudio 3 Real-Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2021Quote: ... and used as a template for qPCR assays employing the SYBR® Premix Ex Taq™ II (Takara) with primers listed in Table S1 ...
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bioRxiv - Biophysics 2021Quote: ... A total of 20 μl reaction system contains 10 μl 2X Premix Ex Taq II (TaKaRa, Dalian, China), 1 μl of 3D gene specific primer (forward ...
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bioRxiv - Molecular Biology 2022Quote: ... Real-time PCR was performed using TB Green® Premix Ex Taq™ II (Tli RNaseH Plus) (Takara). Values of gene expression were normalized to GAPDH expression using the ΔΔCT method.
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bioRxiv - Molecular Biology 2022Quote: ... RT-qPCR for mRNA detection was performed with the SYBR Premix Ex Taq II (Takara Biomedical Technology, China) using Bio-Rad iQ5 detection instrument (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... qRT-PCR was carried out using the SYBR® Premix Ex Taq™ II (Takara Biotechnology, Dalian, China) and CFX96 Connect Real-Time system (Bio-RAD ...
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bioRxiv - Microbiology 2021Quote: ... and the cDNA was analyzed by qRT-PCR using TB Green® Premix Ex TaqTM II (Takara, Japan). The primer sequences are shown in Table 2 ...
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bioRxiv - Plant Biology 2019Quote: ... Real-time quantitative RT-PCR was performed with a PrimeScript II 1st strand cDNA Synthesis Kit (TaKaRa Bio) and SYBR Premix Ex Taq II (TaKaRa Bio) ...
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bioRxiv - Microbiology 2020Quote: ... The qPCR reaction system consisted of 12.5 μL of 2 × SYBR Premix Ex TaqTM II (Takara, Dalian, China), 0.5 μL of upstream and downstream primers (10 mM) ...
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bioRxiv - Genomics 2022Quote: ... in which 5 pmol of substrate primer and 12,5 µl of TB Green Premix Ex Taq II (Takara) were added ...
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bioRxiv - Microbiology 2023Quote: ... each RT reaction solution was amplified in 25 µl of SYBR Premix Ex Taq II (Takara Bio, Inc.) containing 0.2 µM of each primer ...
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bioRxiv - Cancer Biology 2022Quote: ... the complementary DNA (cDNA) products were amplified using TB Green Premix Ex Taq II (Takara Bio Inc, Japan) according to the manufacturer’s instructions in CFX 96 thermo cycler (Bio-Rad Laboratories ...
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bioRxiv - Developmental Biology 2023Quote: ... Purified total RNA was reverse transcribed into cDNA by PrimeScript II 1st strand cDNA Synthesis Kit (Takara, 6210A) or SMARTer™ RACE cDNA Amplification Kit (Clontech) ...
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bioRxiv - Cell Biology 2023Quote: ... and a PrimeScript RTreagent kit with gDNA Eraser and SYBR Premix Ex Taq II (TliRNase H Plus) (TaKaRa) and an ABI7500 qPCR instrument (Life Technologies) ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative PCR reactions were performed using SYBR Premix Ex Taq II with a thermal cycler (TP800, Takara Bio).
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bioRxiv - Biochemistry 2023Quote: ... Real-time polymerase chain reaction (real-time PCR) was performed with TB Green Premix Ex Taq II (Takara). Primers were obtained from Takara Bio Inc ...
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bioRxiv - Molecular Biology 2023Quote: ... Quantitative PCR (qPCR) was conducted in duplicate samples utilizing TB Green Premix Ex Taq II (Takara, Cat# RR820S) and the Thermal Cycler Dice (Takara ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative RT-PCR was then performed on the cDNA using TB Green Premix Ex Taq II (RR820A, Takara), following the instructions manual.
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bioRxiv - Bioengineering 2023Quote: ... qPCR was performed using TB Green® Premix Ex Taq™ II (Tli RNase H Plus) from TAKARA BIO USA INC (CA ...
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bioRxiv - Microbiology 2023Quote: ... The relative mRNA expression was measured by TB Green Premix Ex Taq II (Takara Bio, Shiga, Japan; RR82WR) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... Reverse transcription reactions were performed using the PrimeScript II 1st strand cDNA Synthesis Kit (TAKARA, Cat. No. 9750), solution was then removed ...
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bioRxiv - Neuroscience 2024Quote: ... RT-qPCR was performed using SYBR® Premix Ex Taq™ II (Perfect Real Time, Takara Bio Inc.). SYBR green detection of PCR products was conducted in real time using a MyiQ single-color detection system (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: ... Real time PCR was set up using TB Green Premix Ex Taq II (Tli RNase H Plus) (TakaRa) in a Quantstudio5 machine ...
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bioRxiv - Bioengineering 2019Quote: The promoter of the HSPA6 gene (Uniprot P17066) was amplified from human genomic DNA (Clontech #636401) from −1231 bp to +119 bp relative to the transcriptional start site ...
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bioRxiv - Biochemistry 2019Quote: Human LRAT cDNA was synthesized and cloned into a pcDNA3.1(+) vector (Clontech, Palo Alto, California, USA) by a third party (GeneArt ...
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bioRxiv - Cancer Biology 2019Quote: ... The human patient PDX cell lines were maintained in RPMI-1640 medium containing 10% FBS (Clontech). KRAS mutation status ...
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bioRxiv - Biochemistry 2020Quote: ... Human IFITM3 cDNA was purchased from Open Biosystems and PCR cloned into pCMV-HA vector (Clontech). All mutants of IFITM3 were generated by using QuikChange II Site-Directed Mutagenesis Kit (Agilent Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... for the samples from the mouse heart or Human Mitochondrial DNA Monitoring Primer Set (TaKaRa Bio) for the cells following manufacturer protocol.
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bioRxiv - Genomics 2022Quote: ... 100 ng of polyA selected RNA from the human lung carcinoma cell line A549 (Takara #636141) was used as input ...
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bioRxiv - Physiology 2022Quote: ... the cDNAs of full length human GCNA or IDR hGCNA were cloned into pEGFP-C1 (Clontech) between BglII and SalI sites ...
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bioRxiv - Genetics 2019Quote: Regions orthologous to human placental igDMR were PCR-amplified with TaKaRa EX Taq HS (TaKaRa Bio) with primers specific for chimpanzee and rhesus macaque CGIs (Supplementary Table 4) ...
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bioRxiv - Cell Biology 2020Quote: ... Constructs for C-terminal GFP-tagged human RHBDL4 were generated by subcloning into pEGFP-N1 (Clontech). For generating point mutants ...
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bioRxiv - Cell Biology 2020Quote: Human sparc cDNA clone was described by us before 29 and subcloned into pShuttle vector (Clontech). Adenovirus expressing human SPARC was constructed using Adeno-X expression system (Clontech ...