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Citations for Takara Bio :
151 - 200 of 1405 citations for Human Serum Albumin Rabbit Polyclonal affinity purified since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... Recombinant His-tagged protein was purified from cell-free extracts using immobilized metal affinity chromatography (IMAC using Talon resin; Takara Bio). The buffer used during the purification process was 20 mM Tris-HCl ...
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bioRxiv - Microbiology 2021Quote: ... The recombinant protein in the soluble fraction was then purified using TALON® Metal Affinity Resin (Clontech Laboratories, Mountain View, CA, USA) per the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... or TALON metal affinity resin (Takara), for GST-tagged and His-tagged constructs ...
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bioRxiv - Cell Biology 2021Quote: ... and Talon metal affinity resin (Clontech), respectively ...
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bioRxiv - Biochemistry 2021Quote: ... using TALON metal affinity resin (Takara) (Fridy et al. ...
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bioRxiv - Biochemistry 2023Quote: ... Cobalt-based affinity resins (Talon, Clontech) were used to capture target proteins with an N-terminal His-tag ...
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bioRxiv - Cell Biology 2019Quote: ... 2016) using anti-GFP (Living Colors A.v. peptide antibody, rabbit polyclonal, 1 mg/ml; TakaraBio/Clontech) primary antibody and horseradish peroxidase linked anti-rabbit IgG (from donkey ...
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bioRxiv - Neuroscience 2023Quote: ... a mix of an mCherry-DsRed rabbit polyclonal antibody (Living Colors-Clontech Antibody 632496, 1:1000) and a mouse monoclonal antibody against oxytocin (P38 ...
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bioRxiv - Physiology 2019Quote: ... Membranes were incubated at 4°C in primary antibodies diluted 1:1000 in 5% bovine serum albumin: anti-GFP (ClonTech Living Colours #ab632375), anti-HSP60 (Department of Biology ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were incubated with a diluted primary antibody: rabbit polyclonal anti-DsRed at 1:1000 dilution (Takara Biomedical Technology ...
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bioRxiv - Immunology 2023Quote: Primary antibodies used for imaging include Living Colors anti-DsRed Rabbit Polyclonal Pan Antibody (1:500; TaKaRa), Chicken Polyclonal anti-GFP (1:300 ...
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bioRxiv - Plant Biology 2019Quote: ... and TALON® Metal Affinity Resin (Clontech), respectively ...
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bioRxiv - Microbiology 2021Quote: ... Purification with TALON Metal Affinity Resin (Takara) was carried out from the insoluble fraction by using multiple rounds of sonication and washes in PBS containing 0.1% Tween (v/v) ...
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bioRxiv - Biochemistry 2020Quote: ... Ni-NTA affinity resin was from Clontech. For anion exchange chromatography the HiTrapQ column was from GE Healthcare ...
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bioRxiv - Biophysics 2021Quote: ... Talon metal affinity resin was from Takara Bio (Mountain View ...
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bioRxiv - Microbiology 2023Quote: ... or TALON Metal Affinity Resin (Takara Bio) respectively ...
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bioRxiv - Molecular Biology 2024Quote: TALON Metal Affinity Resin (TAKARA, Shiga, Japan) was used to purify N-terminal Hisx6-tagged TraR protein ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were purified under denaturing conditions by metal affinity chromatography using a column packed with 50% His60 Ni2+ Superflow Resin (Clontech, Mountain View, CA, USA). The purified effector proteins were dialysed (Spectra/Por®3 Dialysis Membrane ...
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bioRxiv - Immunology 2023Quote: ... The 6-His tagged recombinant proteins were purified from the supernatant by gravity-fed through TALON® Metal Affinity Resin (Takara Bio, Shiga, Japan). Following a wash step with PBS (pH 8) ...
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bioRxiv - Biochemistry 2021Quote: ... The resulting cell lysate was used for affinity purification and flowed over a TALON cobalt affinity resin (Takara Bio). The cobalt resin was washed with ~20 column volumes (CV ...
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bioRxiv - Neuroscience 2022Quote: ... or a rabbit anti-DsRed polyclonal antibody (1:1000, Cat # 632496, RRID:AB_10013483, Takara Bio U.S.A., Inc., CA, U.S.A.). In addition ...
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bioRxiv - Genetics 2019Quote: ... Lysis and Talon affinity resin purification (Takara Bio) and size exclusion chromatography was essentially as described [48] ...
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bioRxiv - Biochemistry 2020Quote: ... and TALON® metal affinity resin from Clontech were used ...
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bioRxiv - Cell Biology 2021Quote: ... TALON Metal Affinity Resin (Takara Bio, Catalog #635502) which binds His6-tagged proteins with high affinity ...
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bioRxiv - Molecular Biology 2023Quote: 5 ml TALON metal affinity resin (TaKaRa Bio) was equilibrated with five column volumes of native lysis buffer ...
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bioRxiv - Plant Biology 2023Quote: ... The TALON Metal Affinity Resin (Takara, cat. 635501), Pierce Glutathione Agarose (Thermo ...
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bioRxiv - Genomics 2020Quote: ... and Living Colors DsRed Polyclonal Antibody (rabbit anti-E2-Crimson, Clontech [now Takara Bio USA], Mountain View, CA, USA). Sections were washed and stained with the secondary antibodies Streptavidin:Alexa Fluor 488 (Biolegend ...
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bioRxiv - Neuroscience 2021Quote: ... followed by an incubation overnight at 4°C with antiDsRed rabbit polyclonal primary antibody (1:1000, 632496 Takara Bio) diluted in a triton buffer (0.3% Triton X-100 diluted in PBS-DEPC) ...
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bioRxiv - Neuroscience 2021Quote: ... followed by an incubation overnight at 4°C with antiDsRed rabbit polyclonal primary antibody (1:1000, 632496 Takara Bio) diluted in a triton buffer (0.3% Triton X-100 diluted in PBS-DEPC) ...
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bioRxiv - Biochemistry 2024Quote: ... The recombinant SMC1A HD/RAD21C and SMC3 HD/RAD21N protein complexes were then purified by affinity chromatography using the 10xHis purification tag on RAD21 by incubating the cleared lysates with TALON Metal Affinity Resin (Takara Bio). The purification tag was then removed on the affinity beads by overnight 3C protease digestion at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... Recombinant proteins were extracted from this crude lysate by affinity purification with TALON Affinity Resin (Takara Bio USA, Mountain View, CA, USA), washed with a solution containing 50 mM sodium phosphate ...
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bioRxiv - Neuroscience 2019Quote: ... the slices were incubated overnight at 4°C with a rabbit polyclonal antibody against DsRed (1:1000, Takara Bio USA) diluted in a blocking solution of 0.3% Triton X-100 in PBS-DEPC ...
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bioRxiv - Immunology 2021Quote: ... The following primary antibodies were used in this study: Living colors DsRed Polyclonal antibody (Rabbit, 1:300, Takara Bio #632496); FITC Anti-mouse Ki67 (1:100 ...
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LptM promotes oxidative maturation of the lipopolysaccharide translocon by substrate binding mimicrybioRxiv - Microbiology 2023Quote: ... membranes were incubated with epitope-specific rabbit polyclonal antisera or with an anti poly-histidine horseradish peroxidase-conjugated monoclonal antibody (TaKaRa). Immunodetection was revealed by using a Clarity Western ECL blotting substrate (BioRad ...
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bioRxiv - Biochemistry 2019Quote: ... followed by affinity chromatography using Co Talon resin (Clontech). Further purification was accomplished by ion exchange and size-exclusion chromatography on an AKTA PURE system (GE Healthcare).
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bioRxiv - Biochemistry 2019Quote: ... the supernatant was loaded on TALON affinity resin (Clontech), washed with buffer containing 10mM imidazole and eluted in buffer supplemented with 400mM imidazole ...
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bioRxiv - Biophysics 2023Quote: ... Tandem affinity purification using TALON resin (Takara Bio Inc.) and Streptactin-XT resin (IBA Lifesciences ...
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bioRxiv - Molecular Biology 2024Quote: ... Lysate was incubated with TALON metal affinity resin (Takara) (3ml slurry per L culture ...
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bioRxiv - Biophysics 2024Quote: ... TALON® Metal Affinity Resin was purchased from Takara Bio (Mountain View ...
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bioRxiv - Molecular Biology 2021Quote: ... and loaded onto Co2+-charged TALON Metal Affinity Resin (Clontech) for 1.5 hours with agitation ...
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant was incubated with Talon Metal Affinity resin (Clontech) for one hour or Glutathione S-sepharose 4B (GE Healthcare Life Sciences ...
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bioRxiv - Plant Biology 2021Quote: ... and then applied to TALON metal affinity resin (Clontech, USA). The column was washed with buffer I (0.5 M NaCl ...
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bioRxiv - Biophysics 2020Quote: ... The supernatant was incubated with TALON Metal Affinity Resin (Clontech) for 30 min at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was applied on Talon Metal Affinity Resin (Clontech) pre-equilibrated with the lysis buffer and incubated at 4°C on a rotating wheel for 1 h ...
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bioRxiv - Neuroscience 2021Quote: ... 3% normal goat serum (NGS) and then incubated overnight with 1:2000 rabbit-anti-DsRed (632496, Clontech) (Geerling et al. ...
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bioRxiv - Immunology 2020Quote: ... Transient retroviral supernatants were produced as previously described.44 Activated NK cells were purified and transduced with retroviral supernatants on day +4 in human fibronectin-coated plates (Clontech Laboratories ...
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bioRxiv - Developmental Biology 2022Quote: ... polyclonal (Code No. M142, Takara bio); Monoclonal Anti-N-Cadherin/A-CAM (Clone GC-4 ...
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bioRxiv - Plant Biology 2020Quote: ... and a cobalt-charged affinity resin was used (TALON, Takara Bio).
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bioRxiv - Genomics 2019Quote: ... and proteins extracted with affinity purification using Cobalt-Talon beads (Clontech). Site-directed mutagenesis for Exd and Hth was performed via amplification of the original plasmid with primers harboring single amino acid replacements (arginine to alanine ...
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bioRxiv - Cancer Biology 2020Quote: ... Supernatant was bound to 500μL TALON Metal Affinity Resin (Takara 635501) pre-equilibrated in sonication buffer ...