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Citations for Takara Bio :
601 - 650 of 1209 citations for Human MMP24 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... DNA-lipid complexes were prepared by incubating 100 ng of eGFP-N1 plasmid (Takara Biosciences) with Lipofectamine 2000 for 20 min ...
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bioRxiv - Biophysics 2022Quote: ... This plasmid expresses the PR isoform-B under a tetracycline controllable promoter (TetOff system, Clontech). To perform the SPT experiments ...
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bioRxiv - Neuroscience 2021Quote: ... PCR amplified and inserted into the ESCargo plasmid using in-fusion cloning (Takara Bio, CA). St3-Halo was generated by replacing the GFP in St3-GFP with Halo tag (GenScript USA Inc ...
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bioRxiv - Molecular Biology 2022Quote: ... was amplified from genomic DNA and cloned into a pENTR/D plasmid by InFusion (Takara) to generate pENTR-gTPF2 ...
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bioRxiv - Biochemistry 2022Quote: ... mouse DPPA3 cDNA was sub-cloned into a pGEX4T-3 plasmid using In-Fusion (Clontech) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: ... Mutations of LAT1 were introduced by whole plasmid PCR using PrimeSTAR MAX DNA polymerase (Takara) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... ptrA and AnpyrG used as selective markers were amplified using plasmids pPTR-I (Takara Bio) and pSH1 (14 ...
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bioRxiv - Microbiology 2020Quote: The gammaretroviral transfer plasmids pQCXIP (puromycin resistance) and pQCXIH (pygromycin resistance) were obtained from Clontech. The blasticidin gammaretroviral transfer vector (pQCXIB ...
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bioRxiv - Plant Biology 2021Quote: ... harbouring the pTiBo542 helper plasmid and cloned to pGBKT7 cloning vector (Clontech, Mountain View, USA) (Ramalingam et al ...
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bioRxiv - Cell Biology 2019Quote: ... in the T/C-28a2 chondrocyte cell after transfection with a pIRESpuro2 plasmid construct (Clontech) containing the human Cx43 sequence ...
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bioRxiv - Physiology 2021Quote: ... plasmids were transfected into C2C12 cells using Xfect reagent (Cat. No. 631324; Takara Bio, USA) according to manufacturer instructions.
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bioRxiv - Microbiology 2021Quote: ... and plasmid vectors were amplified by PCR using PrimeSTAR MAX DNA polymerase (Takara, Shiga, Japan) and combined using the In-Fusion System (Takara) ...
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bioRxiv - Molecular Biology 2020Quote: ... HeLa cells were transfected with the following plasmids: pEGFP as control vector expressing GFP (Clontech), and pEGFP-M27 ...
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bioRxiv - Microbiology 2022Quote: Purified plasmids were transfected into HeLa 229 cells using the Xfect transfection reagent (Takara Biosciences) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... plasmid libraries were made using the inverse PCR method with PrimeSTAR Max DNA polymerase (Clontech), In-Fusion HD Cloning Kit (Clontech) ...
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bioRxiv - Microbiology 2022Quote: ... The resulting plasmid was transfected into 293T cells using Lentiviral High Titer Packaging Mix (Takara) to obtain the lentiviral vector for TSG101–venus transduction ...
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bioRxiv - Microbiology 2023Quote: ... USA) and subcloned into the NheI and XhoI restriction sites of plasmid pIRES2-EGFP (Clontech, Takara Bio USA ...
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bioRxiv - Biophysics 2023Quote: ... The plasmid DNA was cut with BamH1 to a linear DNA (Takara Biomedical Technology (Beijing)) ...
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bioRxiv - Systems Biology 2023Quote: ... Ligated plasmid products were transformed into stellar competent cells (E. coli HST08 strain, Takara Bio). See Table S1 for full descriptions of constructs.
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bioRxiv - Cancer Biology 2023Quote: ... The TOB1-AS1 overexpression plasmid was amplified by transforming Stellar™ Competent Cells (Takara, 636763) with the plasmid as per instructions and isolated by QIAGEN HiSpeed Plasmid Midi Kit (QIAGEN ...
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bioRxiv - Developmental Biology 2023Quote: ... to remove the template plasmid DNA and then self-ligated using Ligation High Ver2 (Takara). All PCR products and enzyme-digested vectors were purified using QIAquick PCR Purification Kit (Qiagen) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... coli BL21 (DE3) in the presence of GroEL/ES chaperones (pGro7 plasmid, TAKARA Bio, USA). After a pre-screen on agar plates ...
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bioRxiv - Cell Biology 2023Quote: ... 1.6 µg of a transfer plasmid was transfected into LentiX 293T cells (Clontech Laboratories, inc.) with 0.8 µg of psPAX2 (addgene #12260 ...
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bioRxiv - Synthetic Biology 2024Quote: ... The inducible ABE plasmid was made by integrating a tet-responsive promoter (TRE3G, Takara Bio) and ABE 7.1050 (Addgene #102919 ...
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bioRxiv - Bioengineering 2024Quote: ... and the receptor- or payload-encoding viral expression plasmids (2.0 μg) into Lx293T cells (Clontech) in 6-well plates with Lipofectamine 3000 (Thermo Fisher L3000001) ...
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bioRxiv - Cancer Biology 2024Quote: ... Caspase 8 reporter was established by replacing the DEVD amino acid sequence with XEXD in pLenti_CMV_GFP_Blast_FLIP_GFP_DEVD plasmid through the In-fusion Snap assembly master mix (Takara Bio) using the following oligos forward ...
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bioRxiv - Cancer Biology 2024Quote: 1 x 106 ACKP cells were transfected with 3 µg pE2F-TA-luc plasmid (Takara) and 0.3 µg renilla luciferase control plasmid pRL-CMV (Promega ...
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bioRxiv - Genetics 2024Quote: ... wherein 2.5 µg of pooled plasmid was diluted with Xfect reaction buffer (Takara Bio, 631318) to a total volume of 50 μL ...
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bioRxiv - Cell Biology 2024Quote: Following plasmids were used in this assay to generate stable cell lines: pCMV-HRasG12V (Clontech), Piggyback vectors ...
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bioRxiv - Immunology 2020Quote: ... Activated NK cells were transduced with retroviral supernatants on day +4 in human fibronectin-coated plates (Clontech Laboratories ...
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bioRxiv - Molecular Biology 2019Quote: ... the cDNA was amplified by a polymerase chain reaction from a human brain cDNA library (Clontech, CA) using an appropriate primer pair ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was generated from a human lung poly-A+ RNA (cat. 636105, Takara Bio, Kusatsu, Shiga, Japan) using a cDNA library construction kit (Takara Bio ...
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bioRxiv - Immunology 2020Quote: The pIRES-DsRed2 and pDsRed2-C1 vectors used to express various human aquaporin constructs were from Clontech. The human pCMV6-AC-AQP9-GFP expression vector was from Origene ...
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bioRxiv - Cell Biology 2021Quote: ... the coding sequences of human Pcnt B and Pcnt S were subcloned into peGFP-N1 (Takara Clontech) using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs ...
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bioRxiv - Cell Biology 2021Quote: ... the coding sequences of human Pcnt B and Pcnt S were subcloned into peGFP-N1 (Takara Clontech) using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs ...
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bioRxiv - Immunology 2022Quote: ... cloned into pTT3-based IgG expression vectors with human constant regions (84) using In-Fusion cloning (Clontech), expressed in 293 cells ...
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bioRxiv - Neuroscience 2024Quote: ... Human KCNQ2 cDNA (GenBank accession number NM_172108) in pIRES2-EGFP (BD Biosciences-Clontech, Mountain View, CA, USA) was used as template for in vitro mutagenesis ...
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bioRxiv - Microbiology 2020Quote: ... This DNA fragment was transformed into the ΔasgA strain along with the plasmid pPTR1 (TaKaRa Bio) containing the pyrithiamine resistance ptrA gene ...
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bioRxiv - Genetics 2020Quote: ... The linearized plasmid used for hydrogen peroxide treatment and library construction was cut by EcoRV (Takara) to generate blunt-ends ...
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bioRxiv - Molecular Biology 2021Quote: RNAi plasmids were constructed using the In-Fusion® HD Cloning Kit (Takara Bio; Cat #: 639650) and the L4440 vector ...
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bioRxiv - Neuroscience 2021Quote: ... Neuronal morphology of 14 DIV neurons transfected with the plasmid peGFP-N1 (Clontech, Mountain View, CA) was examined using high-resolution confocal digital images obtained from an Opera-Phenix microscope (Perkin-Elmer) ...
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bioRxiv - Neuroscience 2019Quote: ... and SV40 poly A signal (from the pTRE-tight plasmid from Clontech Laboratories, Mountain View, CA) sequences were cloned right after the 5’ homology fragment ...
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bioRxiv - Cell Biology 2019Quote: ... The mCherry-Golgi plasmid was made by transferring mCherry from pmCherry-N1 into pEYFP-Golgi (Clontech) using AgeI/NotI ...
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bioRxiv - Molecular Biology 2020Quote: ... and further ligated into BbsI digested pX330 plasmids by T4 DNA ligase (Takara; Kusatsu, Shiga, Japan). The ligate was transformed to DH5α competent cells for culture overnight ...
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bioRxiv - Cell Biology 2019Quote: ... This insert was ligated into the HindIII and SalI sites in the peGFP-C1 plasmid (Clontech). The eGFP and COMMD1 coding sequence was subcloned to pTre-TIGHT-Bi (Clontech ...
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bioRxiv - Microbiology 2020Quote: ... All modification for plasmids were performed using overlap PCR mutagenesis with InFusion cloning (Takara Biosciences, USA). Viral genome and support plasmids were maintained in chemically-competent Stbl2 E ...
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bioRxiv - Microbiology 2021Quote: ... This DNA fragment was transformed into the ΔkojR strain along with the plasmid pPTR1 (TaKaRa Bio) containing the pyrithiamine resistance ptrA gene ...
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bioRxiv - Microbiology 2021Quote: ... plasmids respectively using primers HindIII_ZIKV_sfRNA and XbaI_GFP (Supplementary table 7) and PrimeSTAR GXL Polymerase (Takara, Japan). Cycling conditions were as follows ...
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bioRxiv - Microbiology 2022Quote: ... The PCR product and pET28b plasmid were digested with restriction endonuclease EcoRI and HindIII (Japanese Takara), then ligated with T4 DNA Ligase (Japanese Takara) ...
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bioRxiv - Microbiology 2022Quote: ... the FLAG-APEX2-GARG-1060 insert was cloned into the retroviral vector plasmid pLNCX2 (Takara Bio). Cloning details are available upon request ...