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Citations for Takara Bio :
551 - 600 of 5144 citations for Human High Sensitive Interleukin 8 IL8 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Heat Shock Factor 1 (HSF1) as a new tethering factor for ESR1 supporting its action in breast cancerbioRxiv - Cancer Biology 2021Quote: The shRNA target sequence for human HSF1 (NM_005526.4) was selected using the RNAi Target Sequence Selector (Clontech, Mountain View, CA, USA). The target sequences were ...
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bioRxiv - Microbiology 2021Quote: ... Phage libraries were constructed using antibody gene fragments amplified from PBMCs of 50 healthy human subjects (Allcells, PB003F and PB003C) and total RNA from PBMCs (TaKaRa, 636592), spleens (TaKaRa ...
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bioRxiv - Cell Biology 2023Quote: ... The coding sequence of EPHX2 was amplified by PCR from liver cDNA (Human Multiple Tissue cDNA panels; Takara Bio, Shiga, Japan) using primers (5’-GTCGACATGACGCTGCGCGCGGCCGTCTTCG-3’ ...
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bioRxiv - Cell Biology 2023Quote: The plasmid pEGFP-N1–VMP1 was obtained by inserting the human VMP1 sequence (NCBI reference sequence: NM_030938.5) into pEGFP-N1 (CLONTECH cat. #6085-1). The plasmid pcDNA4-B–hVMP1–V5/His was obtained by inserting the human VMP1 sequence (NM_030938.5 ...
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bioRxiv - Developmental Biology 2023Quote: ... Quantitative RT-PCR for pluripotency and trilineage spontaneous differentiation was performed according to the instruction manual of the human ES cell Primer Array (Takara Clontech).
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bioRxiv - Biochemistry 2023Quote: Mouse UCP1 gene and human codon-optimized mitoLbNOX and LbNOX (addgene #74448 and #75285) were cloned into pLVX-TRE3G vector (Clontech, CA) using Gibson assembly ...
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Changes in cell morphology and function induced by NRAS Q61R mutation in lymphatic endothelial cellsbioRxiv - Cell Biology 2023Quote: ... The pLVSIN-EF1α-AcGFP-C1 vector (5.5 μg) was added to 7 μL Lentiviral Mix High Titer Packaging Mix (Takara Bio Inc., Otsu, Japan), 1500 μL serum-free DMEM ...
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bioRxiv - Immunology 2023Quote: ... M-IgA was expressed by transfecting a pair of alpha heavy and kappa light chain genes into FreeStyle CHO-S cells that were grown in a 24-well plate according to the manufacturer’s protocol (CHOgro High Yield Expression System, Takara Bio, Shiga, Japan). For large-scale antibody production ...
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bioRxiv - Cell Biology 2019Quote: ... Calcium phosphate transfection kit (Takara) was used for transfection ...
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bioRxiv - Cancer Biology 2020Quote: ... 3.0 kit (Takara Bio, Inc.) and cloned into the EcoRI/XhoI sites of the pET32a (+ ...
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bioRxiv - Cell Biology 2021Quote: ... SYBR Green PCR kit (Takara) was applied to conduct RT-qPCR ...
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bioRxiv - Genomics 2022Quote: ... Purification Kit Maxi (Takara, 6676) and quantified by the AAVpro Titration Kit (Takara ...
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bioRxiv - Molecular Biology 2019Quote: ... In-Fusion Cloning Kit (Clontech) was used for in vitro assembly of plasmids ...
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bioRxiv - Developmental Biology 2019Quote: ... amplified with SMARTer kit (Clontech), and sequenced on an Illumina HiSeq 4000 platform (100bp runs ...
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bioRxiv - Developmental Biology 2020Quote: ... Dual Index Kit (Takara, # R400406) was used to construct dual indexed libraries for each sample ...
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bioRxiv - Cancer Biology 2021Quote: ... LDH-cytotoxicity assay kit (Takara) was used according to the manual provided ...
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bioRxiv - Immunology 2020Quote: ... with ZapR kit (Takara Bio) for ribosomal depletion ...
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bioRxiv - Cell Biology 2022Quote: ... PrimeScript RT reagent kit (Takara) reverse transcribed RNA and qPT-PCR was performed by SybrGreen Supermix (Bio-Rad Laboratories) ...
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bioRxiv - Developmental Biology 2022Quote: ... amplified with SMARTer kit (Clontech), and sequenced on an Illumina HiSeq 4000 platform (100bp runs ...
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bioRxiv - Neuroscience 2023Quote: ... SMARTScribe Reverse Transcriptase kit (Clontech) was used to generate cDNAs ...
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bioRxiv - Microbiology 2023Quote: ... TaKaRa MutanBEST Kit (Takara, R401), QuickCutTM Hind III (TAKARA ...
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bioRxiv - Developmental Biology 2024Quote: ... ThruPLEX DNA-seq Kit (Takara) was used to construct the CUT&RUN DNA library for sequencing on an Illumina platform ...
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bioRxiv - Molecular Biology 2024Quote: ... or DNA ligation kit (Takara) and transformed into StellarTM competent cells (Takara Bio ...
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bioRxiv - Immunology 2024Quote: ... SMART-Seq HT kit (Takara) was used for full-length cDNA synthesis and amplification followed by library preparation using Illumina Nextera XT kit ...
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bioRxiv - Developmental Biology 2024Quote: ... The SmartSeq v4 kit (Clontech) was used for reverse transcription and cDNA amplification ...
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bioRxiv - Cell Biology 2020Quote: The yeast strain AH109 was co-transfected with bait vector full-length human SNX4 or Lamin cloned into pGBKT7 (Clontech, Oxford, UK) against a pray library of FL SNX-BARs ...
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bioRxiv - Biochemistry 2022Quote: ... DNA ligation of the linearised pOPINTTGneo vector and the human UGGT1insert was achieved by In-fusion™ligation-independent cloning (Takara Ltd.)
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bioRxiv - Neuroscience 2021Quote: ... under control of the modified human GFAP promoter, GfaABC1D (Lee et al., 2008) using restriction cloning and In-Fusion HD assembly (Clontech, Takara Bio, USA). The following sequence elements were obtained from Addgene ...
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bioRxiv - Cancer Biology 2022Quote: ... Plasmid pMSCV-GADD34-puro contains the human PPP1R15A (GADD34, NM_014330.5) gene subcloned into the NcoI/EcoRI sites of the retroviral vector pMSCV-puro (Clontech Laboratories, Mountain View, CA).
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bioRxiv - Immunology 2022Quote: Total RNA was isolated from RAW264.7 cells and ground cartilage from human tibial plateaus using TRIzol reagent (Takara Bio Inc., Shiga, Japan). For mRNA quantification ...
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bioRxiv - Developmental Biology 2023Quote: ... Primer specificity and amplification efficiency for genes of interest were verified by performing reactions on a dilution series of human reference cDNA (Takara, cat# 636693). Gene expression relative to GAPDH and RPLP0 house-keeping genes was calculated in Microsoft Excel ...
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bioRxiv - Immunology 2024Quote: ... genes encoding the variable regions of C7 and C74 heavy chains were cloned into a pMN vector with a human CH1 domain and a C-terminal His-tag using In-Fusion cloning system (Takara Bio #639649). The full light chains of C7 and C74 were cloned into the pMN vector without any purification tag using the same method ...
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bioRxiv - Molecular Biology 2019Quote: ... This region was PCR amplified with DinoSL and KbrSRP-U6R1 primer set (sequences and Tm in Table 2) using the high fidelity PrimeSTAR HS DNA Polymerase (Takara, Kusatsu, Shiga Prefecture, Japan) at 94°C for 1 min ...
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bioRxiv - Cell Biology 2023Quote: ... MSI2 ORF (NM_138962.2) was amplified by PCR with specific primers and high-fidelity Ex Taq DNA polymerase (Takara Bio USA, Inc., Mountain View, CA) using a cDNA containing human MSI2 obtained from OriGene (Rockville ...
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bioRxiv - Molecular Biology 2021Quote: ... The cDNA was synthesized using a reverse transcription system kit (PrimeScript RT Reagent Kit, TaKaRa, RR037B) and oligo(dT ...
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bioRxiv - Systems Biology 2020Quote: ... SMARTer Stranded RNA-Seq Kit (Clontech) was used according to the manufacturer’s instructions to prepare sequencing libraries ...
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bioRxiv - Cell Biology 2020Quote: The In-Fusion Cloning Kit (Takara) and the In-Fusion Cloning primer design tool were used for primer design ...
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bioRxiv - Evolutionary Biology 2021Quote: ... using the In-Fusion Kit (Clontech). The mir-137 and miR-122 sequence was thus under the control of the constitutive human RNA Polymerase III promoter U6.
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bioRxiv - Cell Biology 2021Quote: ... and PCR Clean-Up Kit (Takara) then In-Fusion (Takara ...
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bioRxiv - Plant Biology 2020Quote: ... In-Fusion HD Cloning Kit (Takara) was utilized to construct plasmid vectors ...
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bioRxiv - Microbiology 2020Quote: The SMARTer Pico RNA Kit (Clontech) was used for library preparation ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... PrimeScript RT Reagent Kit (Takara, RR037A) was used to generate cDNA ...
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bioRxiv - Genomics 2020Quote: ... MiniBEST Plant RNA Extraction Kit (TaKaRa) was used to isolate total RNA ...
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bioRxiv - Cell Biology 2020Quote: ... or PrimeSTAR DNA Polymerase kit (Takara). PCR products were run on agarose gel to visualize the fragment size ...
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bioRxiv - Cell Biology 2021Quote: ... Using mycoplasma detection kit (Takara, 6601), we confirmed no contamination in Neuro2A cell line ...
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bioRxiv - Neuroscience 2020Quote: ... a Calcium Phosphate Transfection kit (Clontech) was used to transfect cells with a mixture of OXTR-iTango2 DNA plasmid vectors with a secreted alkaline phosphatase (SEAP ...
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bioRxiv - Microbiology 2021Quote: ... Then the Reverse Transcription Kit (Takara) was used to reversely transcribe RNA to cDNA ...
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bioRxiv - Zoology 2020Quote: ... The protein concentration was measured by TaKaRa BCA Protein Assay Kit (Takara Bio) in accordance with the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... The SYBR Premix ExTaq Kit (TaKaRa) was used for qRT-PCR ...
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bioRxiv - Cancer Biology 2019Quote: ... and cloned using InFusion kit (Clontech) into an expression vector containing fabp10a promoter ...