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Citations for Takara Bio :
551 - 600 of 6770 citations for Human Fibrinogen C Domain Containing Protein 1 FIBCD1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... cell-free protein expression system from Takara Bio Inc ...
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bioRxiv - Biochemistry 2021Quote: ... Protein was purified using Talon resin (Clontech), eluted in 200 mM imidazole ...
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bioRxiv - Zoology 2020Quote: ... The protein concentration was measured by TaKaRa BCA Protein Assay Kit (Takara Bio ...
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bioRxiv - Molecular Biology 2019Quote: ... and protein was incubated with Talon (Clontech), 0.5 ml resin per litre cell culture for 1 h at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... the Capturem Protein A technology from Takara was employed according to the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... subtilis Secretory Protein Expression System (TAKARA Bio). For signal peptide library transformation into B ...
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bioRxiv - Developmental Biology 2021Quote: ... cDNA was synthesized from 1 μg total RNA using a PrimeScript RT reagent kit (Takara) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Total RNA (1 μg) was reverse transcribed by a reverse transcription kit (RR047A, Takara, China). Real-time quantitative PCR was performed on ABI 7500 system (Applied Biosystems ...
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bioRxiv - Genomics 2019Quote: ... 1 microgram of input RNA was used to generate cDNA (Clontech SMARTER cDNA synthesis kit), cDNA was size selected (3-6 kb ...
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bioRxiv - Developmental Biology 2019Quote: ... cDNA was synthesized on 1 μg total RNA using the cDNA synthesis kit (TaKaRa, Japan) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... and 1 μg was reverse-transcribed using PrimeScriptTM RT Reagent Kit (perfect Real Time) (Takara). RT-qPCR was performed with qPCRBIO SyGreen Mix LoRox polymerase (Cultek ...
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bioRxiv - Developmental Biology 2024Quote: ... cDNA libraries were then synthesized using the SMARTer PCR cDNA Synthesis Kit (Clontech; PT4097-1) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... containing 10 μL of TB Green Premix Ex Taq (Takara, Japan), 6 μL of ddH2O ...
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bioRxiv - Microbiology 2020Quote: ... RSV NC was expressed from a pEYFP-N1 containing vector (Clontech) described previously [9] ...
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bioRxiv - Developmental Biology 2022Quote: ... Lentivirus-containing medium was concentrated using Lenti-X-concentrator (Takara Bio). Six-well plates were seeded with 100,000 primary keratinocytes per well and transduced with 150 uL each of lenti-eGFP ...
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bioRxiv - Cell Biology 2019Quote: ... containing 5 μl of 2× SYBR Premix Ex Taq II (TaKaRa), 1 μl of cDNA ...
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bioRxiv - Cell Biology 2019Quote: ... containing 5 μl of 2× SYBR Premix Ex Taq II (TaKaRa), 1 μl of cDNA ...
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bioRxiv - Synthetic Biology 2020Quote: ... coli Rosetta (DE3) (DAD2 constructs) or BL21 (DE3) containing pGro7 (Takara) (ShHTL7 constructs ...
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bioRxiv - Cell Biology 2019Quote: ... Plasmids containing pEGFP-C1 are commercially available from Clontech (CA, USA). Rab5 and Rab11 in the pEGFP-C1 vector were gifts from Prof ...
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bioRxiv - Developmental Biology 2024Quote: ... containing 10% tetracycline-free fetal calf serum (FCS) (TaKaRa Bio, 631106), 2 mM L-Glutamine (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: Expression plasmids for fusion proteins of GFP and tardigrade proteins were constructed by Gibson assembly into pEGFP-N1 (Clontech) of the tardigrade cDNA (obtained by gene synthesis from Integrated DNA ...
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bioRxiv - Molecular Biology 2020Quote: A codon-optimized synthetic gene corresponding to the full length nucleocapsid protein (Thermo GeneArt, Regensburg, Germany) was cloned into a modified pET28a vector using the In-Fusion cloning kit (Takara Bio Saint-Germain-en-Laye, France). The resulting plasmid encoded for an N-terminal His6 tag followed by thrombin and tobacco etch virus cleavage sequences upstream of the full-length nucleocapsid protein sequence ...
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bioRxiv - Evolutionary Biology 2022Quote: ... with 0.1 µL of Taq Polymerase 5 U.µL-1 (TaKaRa Ex Taq® Kit MgCl2 Free Buffer) (TaKaRa Ex Taq, TaKaRa Bio, Shiga, Japan), 2.5 µL PCR Buffer 10X ...
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bioRxiv - Genomics 2019Quote: We used commercially available total RNA from human adipose tissue (Clontech, lot 1604416A) and blood (peripheral leukocytes ...
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bioRxiv - Biochemistry 2020Quote: ... ADAR1 and PCBP2 cDNA were cloned from human thymus plasmid cDNA library (Clontech) using standard PCR techniques and then subcloned into indicated vectors ...
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bioRxiv - Microbiology 2021Quote: The human kidney epithelial cell line Lenti-X 293T was purchased from Takara. The human liver cell line Huh7.5.1 was provided by Dr ...
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bioRxiv - Immunology 2020Quote: The human epithelium kidney 293T Lenti-X cells (Clontech Laboratories, Mountain View, CA) were maintained in Dulbecco’s Modified Eagles medium (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... was inducibly expressed in Lenti-x-293T human female kidney cells from Takara Bio (Catalog # ...
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bioRxiv - Cell Biology 2020Quote: ... S5C-E was generated by fusing human Rac1 cDNA into EGFP-C1 (Clontech) and kindly provided by Dr ...
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Enterohepatic Transcription Factor CREB3L3 Protects Atherosclerosis via SREBP Competitive InhibitionbioRxiv - Physiology 2020Quote: ... mCherry-tagged human SREBP-1c was inserted into pmCherry (mCherry-SREBP-1c) (Clontech), and HA-tagged hamster SCAP ...
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bioRxiv - Molecular Biology 2021Quote: ... was inducibly expressed in Lenti-x-293T human female kidney cells from Takara Bio (Catalog # ...
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bioRxiv - Cancer Biology 2019Quote: Human NIC4 was sub-cloned into pEGFP-N3 (BD Clontech, Mountain View, CA) between EcoRI and BamHI restriction sites to obtain NIC4-GFP using the following primers:
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bioRxiv - Cell Biology 2019Quote: ... The sequences encoding human Rap1a(Q63E) and Rap1GAP1 were cloned into p3xFlag7.1(-) (Clontech). Transient transfection was performed using TransIT-LT1 Reagent (Mirus ...
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bioRxiv - Neuroscience 2020Quote: ... the human cytomegalovirus (hCMV) promoter/immediate-early enhancer (IE) of peGFP-N1 (Clontech) and the chimeric intron (chI ...
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bioRxiv - Cell Biology 2021Quote: ... Primer sequences (Table 2) were verified using total human kidney RNA (Takara Bio). PSMB4 was determined as the most stable housekeeping gene using the method described by Xie ...
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bioRxiv - Immunology 2021Quote: 293T Lenti-X human embryonic kidney cell line (Clontech, Mountain View, CA, USA) was used for LV or IDLV production by transient transfection as previously described22,58 ...
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bioRxiv - Genomics 2022Quote: ... Total RNA pools from human brain (cat. no. 636530, Takara Bio USA, Inc), small intestine (cat ...
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bioRxiv - Biochemistry 2023Quote: ... Human embryonic kidney (HEK) 293T Lenti-X were obtained from Takara (cat# 632180). These cells transformed with adenovirus type 5 DNA and expresses the SV40 large T antigen ...
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bioRxiv - Neuroscience 2023Quote: Human neurons were transfected on DIV28 with pEGFP-C1 (0.3 μg/well, Clontech) plus vectors expressing mCherry ...
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bioRxiv - Biochemistry 2023Quote: ... Lenti-X™ 293T cell line (human, female origin) were purchased from TaKaRa and used without further authentication ...
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bioRxiv - Molecular Biology 2023Quote: ... Cloning of the human SIK2 coding sequence to the pEGFP-C3 (Clontech, USA) vector was previously described by Küser-Abali et al. ...
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bioRxiv - Neuroscience 2024Quote: The primary human fibroblasts (HFs) and the corresponding iPSCs were purchased from Takara Bio (Kusatsu ...
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bioRxiv - Neuroscience 2022Quote: ... slices were incubated in a blocking buffer containing the primary antibodies rabbit anti-mCherry (1:1500; cat. No. 632496, Takara Bio USA, Inc., Mountain View, CA, USA) and chicken anti-GFP (1:1500 ...
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bioRxiv - Cell Biology 2019Quote: ... and both proteins were separated from each other and from a protein encoded by blasticidin resistance gene (cloned from pQCXIB #631516, Clontech) by self-cleavage peptide P2A ...
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Osh6 requires Ist2 for localization to the ER-PM contacts and efficient phosphatidylserine transportbioRxiv - Cell Biology 2020Quote: For testing protein-protein interactions Matchmaker™ GAL4 Two-Hybrid System 3 was used according to the manufacturer’s instructions (Clontech). Briefly ...
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bioRxiv - Bioengineering 2021Quote: ... 1 μg RNA was reverse transcribed using the PrimeScript RT reagent Kit with gDNA Eraser (Takara). The cDNA was then amplified for further analyses using deep sequencing on Illumina NextSeq platform ...
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bioRxiv - Bioengineering 2021Quote: ... RNA (1 μg) was reverse transcribed using the Perfect real-time cDNA reverse transcription kit (TaKaRa). Quantitative PCR was performed with 1 μg of cDNA per sample per target gene using AceQ qPCR SYBR Green master mix (Vazyme ...
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bioRxiv - Immunology 2021Quote: ... cDNA was synthesized from 1 μg of total RNA using an RNA reverse transcriptase kit (Takara). Real-time qPCR was performed using the ABI PRISM 7000 Sequence Detection System (Applied Biosystems) ...
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bioRxiv - Cell Biology 2022Quote: ... Total RNA (1 μg) was reversed to cDNA with PrimeScript™ RT reagent Kit (Takara, RR047Q), which was performed according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA was prepared with 1 µg RNA and synthesized using the Primescript RT kit (Takara Inc.). qPCR assays were performed on the StepOnePlusTM Real-time PCR System (Applied Biosystems ...