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Citations for Takara Bio :
201 - 250 of 551 citations for Hemopexin Mouse HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... coated T-225 flasks containing 85-95% confluent HEK-293T/17 cells (ATCC, CRL-11268) were each transfected (Xfect, Takara #631318) with a DNA cocktail containing the 1 ...
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bioRxiv - Neuroscience 2023Quote: ... Supernatants from rescue plates were passaged on 15 cm plates of HEK 293T/17 cells (ATCC) transfected with pLV-TTBG and pLV-TTBL (described above) using Xfect transfection reagent (Takara 631318) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: Plasmids NLAD8 HIV-1 AD8 Macrophage-Tropic R5 (NIH AIDS reagents #11346) or HIV Gag-imCherry V3 loop (29) were transfected in HEK 293T cells using CalPhos Mammalian Transfection Kit (Takara Bio) in DMEM ...
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bioRxiv - Biophysics 2019Quote: ... Cells were harvested and purified using TALON His-Tag Purification protocol (Clontech Laboratories, Mountain View, CA.) The SUMO tag was cleaved by Ulp-1 as described above and the protein was further purified using ion-exchange chromatography (Bio-Rad ...
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bioRxiv - Plant Biology 2020Quote: ... and on a SD-Leu-Trp-Ade-His plate containing X-α-gal (Takara Bio, USA). Plates were imaged after incubation for 60 - 72 hr at 30°C unless otherwise stated ...
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bioRxiv - Plant Biology 2020Quote: ... The E.coli-expressed tNPR1-His protein was extracted and purified using TALON Metal Affinity Resin (Clontech). For rabbit immunization ...
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bioRxiv - Genetics 2022Quote: ... then washed in water and re-suspended in 125-250mL -leu - his galactose liquid culture (Takara Bio Minimal SD Bases ...
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bioRxiv - Molecular Biology 2019Quote: ... the fragment were ligated into the Xho I and Bam HI sites of pEGFP-N3 (Clontech), creating Aβ fused in frame to the N-terminus of GFP ...
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bioRxiv - Neuroscience 2023Quote: ... pAcGFP-His-MAP2C without AcGFP was amplified and Dendra2 was amplified from pDendra2-C vector (Takara). Dendra2 was inserted into where AcGFP was by In-Fusion cloning kit ...
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bioRxiv - Cell Biology 2023Quote: ... His-tagged recombinant proteins were isolated by 1 h incubation with Talon metal affinity resin (Clontech) at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... HEK293T cells grown in 10 ml of medium (3.0 x 105 cells/ml) were transfected with 10 µg of pCAGGS-NP (1-450) using TransIT-293 Reagent (Takara, Shiga, Japan). Three days post-transfection ...
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bioRxiv - Cancer Biology 2023Quote: ... and 1.25Lμg pVSV-G (second-generation lentivirus packaging system) into human embryonic kidney packaging cells GP2-293 (Clontech, Inc., Mountain View, CA, USA), using CalPhos™ Mammalian Transfection Kit (Clontech ...
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bioRxiv - Microbiology 2023Quote: ... packaging cells were transfected with expression vectors (pMSCVneo-fePit1, pMSCVneo-fePit2, or pMSCVneo empty vector) using the TransIT®-293 reagent (Takara, Kusatsu, Japan). Two days later ...
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bioRxiv - Plant Biology 2019Quote: Each purified protein preparation of His-PHOT1 N2 and N4 was incubated with TALON Magnetic Beads (TaKaRa) at 4°C for 30 min and further incubated at 4°C for 30 min with in vitro transcription and translation reactant containing RPT2 N ...
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bioRxiv - Cell Biology 2022Quote: ... The library was generated by using the SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian (Clontech) with 1 μg RNA as input ...
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bioRxiv - Microbiology 2020Quote: ... the His-tagged spike protein produced in the culture supernatants was purified with a Talon resin (Clontech).
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bioRxiv - Pathology 2020Quote: ... RBD-scFv was purified from the supernatant using Capturem™ His-Tagged Purification Miniprep Kit (Takara Bio). One prep of 800 μL supernatant through one column of the kit yielded 102 μg/mL of RBD-scFv measured by NanoDrop™ 2000/2000c Spectrophotometers (ThermoFisher) ...
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bioRxiv - Molecular Biology 2020Quote: ... Fractions were precipitated with trichloroacetic acid (TCA) and subjected to Western blot analysis (anti-His antibody, Clontech). Additionally ...
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bioRxiv - Developmental Biology 2020Quote: ... Both chicken DLX1 and DLX1 Q50E proteins were purified using Capturem His-Tagged Purification Maxiprep Kit (Clontech), and verified by Western blot with primary antibodies against 6x His tag (Invitrogen ...
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bioRxiv - Bioengineering 2023Quote: ... Yeast transformants were selected by growth on synthetic dropout nutrient medium SD/-Leu/-His/-Ade/-Trp (Clontech) supplemented with 40mg/LX-α-gal (5-bromo-4-chloro-3-indolyl-a-D-galactopyranoside ...
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bioRxiv - Developmental Biology 2021Quote: ... Lentiviral supernatants were subsequently collected after transfection of HEK 293T cells with either the pLVX-TRE3G-GOI or the pLVX-Tet3G vector using the X-fect reagent (Clontech; Cat. No. 631317). Supernatants were concentrated by ultracentrifugation ...
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bioRxiv - Bioengineering 2023Quote: ... CCR5-KO donor rAAV6 virus was produced in HEK-293T cells and was purified with AAVpro Purification Kit (TakaRa, San Jose, CA, USA). Electroporation of the RNP complex was performed using the Lonza 4D-Nucleofector (Lonza Group Ltd ...
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bioRxiv - Cancer Biology 2023Quote: Lentiviral particles were generated in HEK 293T cells by co-transfection of the lentiviral vector pLVX-luc-IRES-puro (Clontech, Mountain View, CA) with the pCMV-dR8.9 and pCMV-VSV-G plasmids (both obtained from Addgene ...
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bioRxiv - Plant Biology 2022Quote: ... and His (-HTLA) but containing 5-bromo-4-chloro-3-indolyl α-D-galactopyranoside (Clontech, Madison, WI, USA). As a control ...
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bioRxiv - Microbiology 2022Quote: ... then the His-tagged ORF8 protein produced in the culture supernatants was purified with a Talon resin (Clontech). The absence of endotoxin contamination was confirmed using the ToxinSensor chromogenic LAL Endotoxin Assay Kit (GeneScript).
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bioRxiv - Molecular Biology 2023Quote: ... and RNA-sequencing libraries were made using the SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian (Takara). Libraries were sequenced at the Bauer Core Facility (Harvard University ...
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bioRxiv - Cell Biology 2023Quote: ... RNA-seq libraries were prepared with the SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian (Takara #634875). 75 bp single-end sequencing was performed using an Illumina NextSeq500 system at the CRI at UT Southwestern Sequencing Facility ...
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bioRxiv - Plant Biology 2023Quote: ... Ade and His but containing 5-Bromo-4-Chloro-3-indolyl a-D-galactopyranoside (X-a-gal) (Clontech). To detect interactions ...
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bioRxiv - Genomics 2023Quote: ... Libraries were prepared using the SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian (Takara Bio USA, Inc.), which incorporates both RiboGone and SMART (Switching Mechanism At 5’ end of RNA Template ...
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bioRxiv - Genomics 2023Quote: ... 100 ng to 1 µg of total RNA was used for Hi-Mammalian whole transcriptome preparation (Takara Bio) and sequencing was performed on Nextseq2000 instrument with 1 x 72 bp single-end setup ...
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bioRxiv - Microbiology 2023Quote: ... The His-tagged CRONE protein was purified by cobalt affinity chromatography using TALON® metal affinity resin (Takara) under native conditions in phosphate buffered saline (PBS) ...
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bioRxiv - Cell Biology 2023Quote: ... Sequencing libraries from treated RNA were created using the SMARTer Stranded Total RNA HI Mammalian kit (Takara 634873) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA-seq libraries were prepared using the SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian kit (Takara) due to retirement of the ScriptSeq v2 kit ...
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bioRxiv - Biophysics 2021Quote: ... Constructs containing both His and Strep tags were purified using gravity flow columns containing His60 Ni-NTA resin (Clontech) followed by Streptactin affinity chromatography (IBA Lifesciences ...
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bioRxiv - Microbiology 2019Quote: ... falciparum constructs were cloned into Nhe I-Bam HI sites of the pIRES2-EGFP vector (Takara Bio, formerly Clontech). All constructs contain a modified murine kappa chain secretion signal peptide and C-terminal His tag [18] ...
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bioRxiv - Microbiology 2019Quote: ... falciparum constructs were cloned into Nhe I-Bam HI sites of the pIRES2-EGFP vector (Takara Bio, formerly Clontech). All constructs contain a modified murine kappa chain secretion signal peptide and C-terminal His tag [18] ...
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bioRxiv - Microbiology 2020Quote: ... lysed by sonication and the recombinant His-tagged protein purified from cell-free extracts using IMAC (Talon resin, Clontech) as previously described19.
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bioRxiv - Molecular Biology 2020Quote: ... Samples were analyzed on bleach agarose gels (0.06% bleach, 1% (w/v) agarose) for visualization of the RNA and on WB (anti-His antibody, Clontech) for visualization of His6-tagged Nsp1.
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bioRxiv - Synthetic Biology 2021Quote: ... Samples containing the thereby secreted [His]6-tagged VHH-HlyA fusions were next passed through Talon CellThru resin (Clontech). For this ...
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bioRxiv - Immunology 2021Quote: ... His-tagged NTD domain constructs were purified from clarified supernatants using 2 ml of cobalt resin (Takara Bio TALON), washing with 50 column volumes of 20 mM HEPES-HCl pH 8.0 and 150 mM NaCl and eluted with 600 mM imidazole ...
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bioRxiv - Molecular Biology 2023Quote: ... and verified whether it has self-activation reaction through synthetic dropout (SD)-Ade-His-Leu-Trp (Clontech, CA, USA) with X-α-galactosidase (x-α-gal) ...
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bioRxiv - Plant Biology 2022Quote: ... and interaction was examined on SD-Ade/-His/-Leu/-Trp quadruple dropout medium (Clontech, Cat. No. 630317 and 630323). For Y3H assays ...
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bioRxiv - Immunology 2023Quote: ... Samples were rRNA depleted and prepared for sequencing using SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian (Takara). In brief ...
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bioRxiv - Plant Biology 2023Quote: ... and SD4 (-Trp-Leu-His-Ade) media at 30℃ for 3–5 d according to the manufacturer’s manual (Clontech).
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Essential roles of the ANKRD31-REC114 interaction in meiotic recombination and mouse spermatogenesisbioRxiv - Genetics 2023Quote: ... alone or also lacking adenine and histidine (SD-Trp/Leu/Ade/His/AbA) were performed following the manufacturer’s instructions (Clontech).
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bioRxiv - Immunology 2023Quote: ... S-IgA was purified by two-step chromatography using the Capturem His-Taged Purification Kit (Takara Bio, Shiga, Japan) followed by size exclusion chromatography (Cytiva ...
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bioRxiv - Plant Biology 2023Quote: ... Approximately 2.6 x 106 yeast transformants were screened on 2% SD/Gal/Raf/X-P-gal (-Ura/-His/-Trp/-Leu) following the manufacturer’s instructions (Clontech). Direct protein-protein interaction was confirmed by co-transformation of the respective plasmids into the yeast strain AH109 using the Matchmaker GAL4 Two-hybrid System (Clontech) ...
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bioRxiv - Cancer Biology 2023Quote: ... His-TEV tagged protein was captured with Co-TALON resin (Clonetech, Takara Bio USA, 2 mL slurry/liter culture) at 4 ºC for 1 h with constant end-to-end mixing ...
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bioRxiv - Neuroscience 2021Quote: ... MYC (mouse, Clontech, 631206 ...
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bioRxiv - Molecular Biology 2020Quote: ... One µg of total RNA was used as input for SMARTer Stranded Total RNA Sample Prep Kit-HI Mammalian (Clontech). Sequencing was performed on the NextSeq500 instrument (Illumina ...