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Citations for Takara Bio :
1 - 50 of 180 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... and histidine (H) and containing 5-Bromo-4-Chloro-3-Indolyl α-D-galactopyranoside (X-α-gal) (Clontech) to detect interactions ...
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bioRxiv - Cell Biology 2022Quote: ... D/D solubilizer (635054) from Takara, VECTASHIELD Mounting Medium (H-1400 ...
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bioRxiv - Cell Biology 2020Quote: ... D/D solubilizer were purchased from Clontech. Methyl-β-cyclodextrin and 2-hydroxypropyl-β-cyclodextrin were purchased from Wako Chemicals ...
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bioRxiv - Genomics 2020Quote: Ribonuclease H (RNase H) (20~60 U/μL) (Takara Bio Inc)
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bioRxiv - Genomics 2023Quote: ... The final 65 μl volume was treated with 5 μl of an enzymatic mixture containing: 0.1 μl of RNase H (60 U/μl, Ribonuclease H
H>, catalog num. 2150, Takara), 2 μl of RNase ONE (10 U/μl ... -
bioRxiv - Cell Biology 2023Quote: 1.0 μM of D/D solubilizer (Takara, Cat: # 635054) in Opti:MEM® (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated with D/D-Solubilizer (5 μM, Clonetech/Takara) and Cycloheximide (35.54 μM ...
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bioRxiv - Plant Biology 2023Quote: ... and histidine (-H) (TaKaRa 630319).
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bioRxiv - Cell Biology 2021Quote: ... 3 h and 0 h before extracting total RNA by MiniBEST Universal RNA Extraction Kit (Takara). The abundances of the interest genes were detected measured in each time point by real-time quantitative PCR (qPCR ...
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bioRxiv - Cell Biology 2023Quote: ... The human WT Trop-2 and the Trop-2-Q118E mutant cDNAs [70] were amplified by PCR method (primers: F’ gcgattctcgagtccggtccgcgttcc-XhoI and R’ gcgccggtaccaagctcggttcctttc-KpnI) and sub-cloned into the pEYFP-N1 vector (Clontech, OH, USA) for mammalian expression to give the Trop-2-pEYFP-N1 and Trop-2-Q118E-pEYFP-N1 plasmids.
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bioRxiv - Cell Biology 2023Quote: ... Release of TfR from the ER was triggered by adding D/D solubilizer (Takara Bio) to the live-cell imaging solution at a final concentration of 1 µM.
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bioRxiv - Cell Biology 2020Quote: PC4 cells (Gordon et al., 2017) were incubated with 0.5 µM D/D Solubilizer (Takara; 635054) in a time course-dependent manner ...
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bioRxiv - Developmental Biology 2021Quote: ... Lentiviral supernatant was harvested at 48 h and 54 h after transfection and concentrated using the lenti-X concentrator (Clontech) at 4°C for 12-36 h ...
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bioRxiv - Genomics 2024Quote: ... RNA was digested with RNase H (Takara Bio) at 37°C for 30 min and the cDNA was purified with AMPureXP beads ...
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bioRxiv - Neuroscience 2023Quote: ... The virus particles containing media were collected 24 h and 48 h after transfection and were concentrated using Lenti-X concentrator (Takara, 631232). The virus containing media was resuspended in Neurobasal media and tittered by puromycin selection using HEK 293T cell line ...
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bioRxiv - Neuroscience 2024Quote: ... HEK293 cells were first incubated with ARIAD ligand at a concentration of 1 µM (AL; D/D Solubilizer; Takara) for the indicated times ...
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bioRxiv - Microbiology 2021Quote: ... P3 and NP from D/660 and D/CN286 were amplified from the respective bidirectional pHW2000 plasmid constructs using CloneAmp Hifi PCR mix (Takara) with the following cycle profile ...
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bioRxiv - Cell Biology 2020Quote: ... generates a protein that undergoes spontaneous aggregation that reverses upon the addition of D/D solubilizer (catalogue# 635054; Takara Bio), a cell-permeant rapamycin analog that binds to the FM4 domains (see Fig 3e).
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bioRxiv - Synthetic Biology 2020Quote: ... The deletion of the lacZ gene was verified using blue-white selection on an LB agar plate containing 0.1 mM isopropyl-β-D-thiogalactopyranoside (Nacalai Tesque) and 4.0 × 10−3 % 5-bromo-4-chloro-3-indolyl-β-D-galactoside (Takara Bio).
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bioRxiv - Biophysics 2022Quote: ... HEK-D (see below) and Lenti-X 293T (Takara) cells were cultured in growth medium consisting of Dulbecco’s modified Eagle’s medium (GIBCO) ...
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bioRxiv - Molecular Biology 2021Quote: ... Resulting cDNAs were treated with RNAse H (Cat#2150B; Takara Bio) and quantified by RT-qPCR using the PowerUp™ SYBR™ Green Master mix (Cat#A25742 ...
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bioRxiv - Genetics 2021Quote: ... SYBR Premix Ex Taq II (Tli RNase H Plus) (Takara Bio), or Power SYBR Green PCR Master Mix (Applied Biosystems ...
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bioRxiv - Plant Biology 2024Quote: ... TB Green Premix Ex Taq II (Tli RNase H Plus) (Takara) was used for qPCR analysis in an Mx3000P qPCR machine (Agilent) ...
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bioRxiv - Biochemistry 2023Quote: ... TB Green Premix Ex Taq II (Tli RNase H Plus) (Takara, #RR820A), RevertAid First Strand cDNA synthesis kit (Thermo scientific ...
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Vitamin D signaling orchestrates skeletal muscle metabolic flexibility by regulating its fuel choicebioRxiv - Physiology 2023Quote: ... with SYBR® Premix Ex Taq (Tli RNase H Plus) by TAKARA. Each sample was amplified in triplicates using primers specific to genes of interest ...
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bioRxiv - Molecular Biology 2024Quote: ... TB Green® Premix Ex Taq™ (Tli RNase H Plus; TAKARA) in a set volume of 10µL ...
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bioRxiv - Plant Biology 2024Quote: ... and 1×SYBR Premix Ex Taq (Tli RNase H plus, RR420A; Takara). PCR conditions were as follows ...
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bioRxiv - Developmental Biology 2021Quote: ... The supernatant was collected after 48 h and the Lenti-X concentrator (Clontech) was added to the suspension ...
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bioRxiv - Cell Biology 2020Quote: ... After 24 h cells were transfected with an eGFP vector (3 μg; Clontech) using Fugene-6 HD reagent according to the manufacturer's protocol ...
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bioRxiv - Genetics 2021Quote: ... or using SYBR Premix Ex Taq II (Tli RNase H Plus) (Takara Bio) on a LightCycler 480 II (Roche) ...
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bioRxiv - Cell Biology 2024Quote: ... device using TB Green Premix Ex Taq II (Tli RNase H Plus, Takara) and the gene expression was normalized to Cyclophilin and presented ...
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bioRxiv - Neuroscience 2023Quote: ... fixed for 1 h in 4% paraformaldehyde/PBS (TAKARA BIO INC. Cat#T900), and subjected to antibody labeling ...
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bioRxiv - Developmental Biology 2024Quote: ... 5 µl of SYBR® Premix Ex Taq (Tli RNase H Plus) (Takara) and run in a CFX connect instrument (Bio-Rad) ...
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bioRxiv - Developmental Biology 2024Quote: ... 5 µl of SYBR® Premix Ex Taq (Tli RNase H Plus) (Takara) and run in a CFX connect instrument (Bio-Rad) ...
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bioRxiv - Genetics 2024Quote: ... We used TB Green Premix Ex Taq II (Tli RNase H Plus) (TAKARA) to perform quantitative real-time PCR on LightCycler LC480 (Roche ...
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bioRxiv - Microbiology 2020Quote: ... D and E using high fidelity PrimeSTAR Max DNA Polymerase (Takara). T7 promoter was introduced upstream of 5’ UTR of SARS-CoV-2 genome in fragment A ...
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bioRxiv - Plant Biology 2020Quote: ... After 4 h total RNA was extracted using RNAiso Plus (Takara Bio, Shiga, Japan) according to the manufacturer’s protocol and used for real-time quantitative RT-PCR (qRT-PCR ...
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bioRxiv - Cell Biology 2020Quote: ... the reverse transcription reactions were performed using reverse Transcriptase M-MLV (RNase H-) (TaKaRa). The cDNA was then used for real-time qPCR using the SYBR Green qPCR Master Mix (Bi-make ...
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bioRxiv - Biochemistry 2022Quote: ... The dialyzed fraction was mixed for 3 h with Talon Metal Affinity Resin (Clontech) that had been equilibrated with Buffer T ...
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bioRxiv - Microbiology 2020Quote: Lenti-X 293T™ cells (H. sapiens; sex: female) obtained from Takara (cat. #632180) and Huh-7.5 cells (H ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA was labelled with SYBR Pre-mix Ex Taq (Tli RNase H Plus, Takara) and the Cq values were obtained from the CFX96 TouchTM Real-Time PCR Detection System (BioRad) ...
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bioRxiv - Developmental Biology 2024Quote: ... and 5 µl of SYBR® Premix-Ex-Taq (Tli RNase H Plus, Takara) in a 10 µl reaction ...
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bioRxiv - Cell Biology 2021Quote: ... Lentiviral particles were collected 72 h after transfection and concentrated with Lenti-X Concentrator (Clontech) and stored at − 80°C ...
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bioRxiv - Cell Biology 2020Quote: ... and TB Green™ Premix Ex Taq™ II (Tli RNase H Plus) (RR820L, Takara) with two repeats for each PCR ...
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bioRxiv - Molecular Biology 2023Quote: ... and SYBR Premix Ex Taq II (Tli RNase H Plus) (Takara Bio INC, Kusatsu, Japan). All reactions were performed in at least three biological replicates ...
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bioRxiv - Plant Biology 2024Quote: ... were diluted by a 10x series dilution and spotted onto –L/-T/-H medium (Clontech) to determine interactions between the two proteins.
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bioRxiv - Microbiology 2023Quote: ... The cDNA was amplified using SYBR Premix Ex Taq II (Tli RNase H Plus; Takara) in a CFX96 Touch real-time PCR detection system (Bio-Rad ...
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bioRxiv - Plant Biology 2022Quote: ... The HMWG::TaVIT2-D sequence was inserted by In-Fusion cloning (Takara Bio) in the HindIII restriction site upstream of ZmUBI1::OsNAS2 ...
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bioRxiv - Cell Biology 2020Quote: The GST-Bbs5 was expressed for 3 h at 27 °C in Escherichia coliBL21(DE3) (Takara) transformed with pGEX-4T-2-Bbs5-WT in the presence of 0.1 mM isopropyl-β-D-thiogalactopyranoside (Takara) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Membranes were baked for 2 h at 80°C and prehybridized in ExpressHyb (Clontech, Mountain View) at 65°C for 1h ...