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Citations for Takara Bio :
1 - 50 of 359 citations for GDF 11 BMP 11 Human HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: Hippocampal neurons were transfected at 11 days in vitro (DIV 11) using Calphos calcium phosphate transfection reagent (Takara) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... Primary antibodies anti-HA.11 (Clontech, 631207) or anti-A3H were used at a dilution at 1:500 and 1:50 ...
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bioRxiv - Microbiology 2022Quote: ... HEK293 cells were cotransfected with the S expression plasmids (400 ng) and pDSP8-11 (400 ng) using TransIT-LT1 (Takara, Cat# MIR2300). To prepare target cells ...
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bioRxiv - Genomics 2021Quote: ... 11 µL of 10 mM dNTP (Takara, #639125), 13.75 µL of ultra-pure water and 2.75 µL of RNase Inhibitor (Fisher ...
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bioRxiv - Biochemistry 2021Quote: ... TB Green Premix Ex Taq 11 (TliRNaseH Plus) kit (Takara, Japan) with respective qRTPCR conditions (Annealing Tc-55°C or 60°C ...
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bioRxiv - Cell Biology 2020Quote: Human Embryonic Kidney 293 (HEK293) cells (Clontech Laboratories) were used for most experiments described in this study ...
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bioRxiv - Developmental Biology 2022Quote: ... Quantitative (q) RT-PCR was performed using SYBR Premix ExTaq 11 (Takara Bio) and detected using the Bio-Rad CFX96 Connect system ...
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bioRxiv - Biochemistry 2022Quote: ... as used before.11 TSC were cotransfected with GFP-expressing plasmid peGFP-CI (Clontech) and sorted for GFP-positive cells in a 96 well plate at the central research facility cell sorting at MHH ...
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bioRxiv - Microbiology 2021Quote: ... stomach and trachea of the 11 species were extracted using RNAiso Plus (TaKaRa, Lot#AJF1819A), and reverse-transcribed using TaKaRa PrimerScript TM II 1st Strand cDNA Synthesis Kit (Lot#AK70993A) ...
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bioRxiv - Neuroscience 2023Quote: ... which was inserted into pAcGFP-His- MAP2C 11-308 by In-Fusion cloning kit (Takara). pAcGFP-His-MAP2C-Tau was chimera of MAP2C_M1-L311 and Tau0N4R_P193-L383 in the numbering Tau0N4R ...
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bioRxiv - Biochemistry 2022Quote: ... 9, 11, 13, 20, or 45 (SERF2, C9orf16, C19orf53, C11orf58, BEX3, or SERBP1 respectively) was inserted into pCold I (Takara), together with the client protein ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA was amplified by PCR in separate 25 μL reactions each containing 2 μL of cDNA for 11-13 cycles using 0.5 μL (2.5 U) LA Taq (Takara, Cat# RR002M) with P5-3’ and miRCat-PCR-2 oligos at an annealing temperature of 58°C ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The cDNA samples were cloned into a pUC19 vector (PCR-amplified with primers 11 and 12) using In-Fusion HD Cloning Kit (Takara). After transformation into Escherichia coli ...
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bioRxiv - Biophysics 2024Quote: ... was cloned from the Saccharomyces cerevisiae S288c genome into a pETM-11 expression vector linearised at the XhoI and XbaI restriction sites using In-Fusion (Takara) technology ...
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bioRxiv - Molecular Biology 2020Quote: ... JEG3 cells (human; sex: female, placenta epithelial), HEK293 derivative Lenti-X™ 293T cells (human; sex: female, kidney epithelial) obtained from Takara (cat. 632180), Huh-7.5 heptoma cells (human ...
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bioRxiv - Plant Biology 2022Quote: Full-length CDSs of CiS40-11 was cloned into the expression vector pCanG-HA and pCAMBIA1302 using In-Fusion® HD Cloning Kit (TaKaRa, Japan). All generated binary plasmids were confirmed by enzyme digestion and sequencing validation ...
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bioRxiv - Microbiology 2022Quote: ... HEK293 cells were cotransfected with the S expression plasmids (400 ng) and pDSP8-11 (ref.65) (400 ng) using TransIT-LT1 (Takara, Cat# MIR2300). On day 3 (24 hours posttransfection) ...
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bioRxiv - Microbiology 2022Quote: ... HEK293 cells were cotransfected with the S expression plasmids (400 ng) and pDSP8-11 (ref.58) (400 ng) using TransIT-LT1 (Takara, Cat# MIR2300). On day 3 (24 hours posttransfection) ...
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bioRxiv - Microbiology 2023Quote: ... HEK293 cells were cotransfected with the S expression plasmids (400 ng) and pDSP8-11 (ref.69) (400 ng) using TransIT-LT1 (Takara, Cat# MIR2300). On day 3 (24 hours post-transfection) ...
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bioRxiv - Microbiology 2023Quote: ... HEK293 cells were cotransfected with the S expression plasmids (400 ng) and pDSP8-11 (ref.59) (400 ng) using TransIT-LT1 (Takara, Cat# MIR2300). On day 3 (24 h posttransfection) ...
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bioRxiv - Cell Biology 2023Quote: The pik1-11 temperature sensitive allele was constructed as described (Tang et al., 2019) with the exception that EX taq polymerase (Takara, cat# 4025) and accompanying dNTPs (Takara ...
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bioRxiv - Synthetic Biology 2023Quote: ... HEK293 cells (Takara 632180), C3H/10T1/2 Clone 8 (ATCC# CCL-226) ...
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bioRxiv - Genetics 2019Quote: ... RNA was reverse transcribed using SMART-Seq Reverse Transcriptase followed by 11 cycles of PCR amplification (SMART-Seq v4 kit, Takara Bioscience, Cat. 634890). Purification and size selection of DNA was carried out using Ampure XP beads (Beckman Coulter ...
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bioRxiv - Molecular Biology 2019Quote: HEK293 Tet-off cells (Clontech) were transfected with pTRE-TIGHT plasmids bearing the (de)optimized and WT sequences and incubated overnight at 37ºC ...
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bioRxiv - Biophysics 2021Quote: ... HEK293 Tet-ON cells (Clontech) were co-transfected with linearized pTRE-HTT128Q together with a plasmid expressing a hygromycin resistance gene ...
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bioRxiv - Neuroscience 2020Quote: We transiently co-transfected cDNA constructs of GluN1 and GluN2A into mammalian human embryonic kidney 293 (HEK293) with a separate pEGFP-Cl vector (Clontech) at a ratio of 4.5:4.5:1 (GluN1/GluN2A/EGFP ...
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bioRxiv - Cell Biology 2020Quote: ... 7.5 × 107 Hek293-lentiX cells (Clontech) were seeded on 15-cm tissue culture plates ...
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bioRxiv - Cancer Biology 2019Quote: HEK293 Lenti-X (Clontech, Cat. # 632180) cells were cultured in DMEM with 10% FBS ...
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bioRxiv - Cell Biology 2023Quote: HEK293 (Clontech #C3003-1; lot #7030396) cell lines were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) ...
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bioRxiv - Biophysics 2021Quote: ... HEK293S Lenti-X producer cells (Takara/Clontech) were transfected in DMEM with 2 % (v/v ...
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bioRxiv - Biophysics 2021Quote: ... HEK293S Lenti-X producer cells (Takara/Clontech) were transfected in DMEM with 2 % (v/v ...
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bioRxiv - Bioengineering 2023Quote: HEK293 cells (DMSZ) and HEK293T cells (Takara) were maintained in Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Immunology 2019Quote: ... HEK293 cells were transfected using Xfect (TakaRa, #631318) with the viral packaging construct [pMDLg/pRRE (Addgene ...
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bioRxiv - Cell Biology 2022Quote: ... Lenti-X HEK293 cells (Takara Bio, Cat #632180) were transfected with pHR-SFFV-dCas9-BFP-KRAB ...
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bioRxiv - Cancer Biology 2019Quote: ... embryonic kidney cell line HEK293(ATCC CRL-1573) and 293GP cells (Clontech) were cultured in high glucose (5g/l ...
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bioRxiv - Immunology 2023Quote: ... CPER products were transfected into HEK293-C34 cells using TransIT-LT1 (Takara) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... HEK293 cells were co-transfected either with pEGFP-N1 (Clontech, 6085-1) or pCAG-DsRed-T1 (gift from Prof ...
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bioRxiv - Cell Biology 2021Quote: ... The HEK293-based Lenti-X 293T (HEK293T) cell line was obtained from Takara Bio (Shiga ...
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bioRxiv - Cell Biology 2022Quote: ... Virus containing supernatants were harvested five times from transfected Ecopack-HEK293 cells (Clontech/Takara), over a period of three days post-transfection and filtered with 0.45 µm filters ...
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bioRxiv - Cell Biology 2022Quote: ... Virus containing supernatants were harvested five times from transfected Ecopack-HEK293 cells (Clontech/Takara), over a period of three days post-transfection and filtered with 0.45 µm filters ...
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bioRxiv - Microbiology 2022Quote: ... the CPER products were transfected into HEK293-C34 cells using TransIT-LT1 (Takara, Cat# MIR2300) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... the CPER products were transfected into HEK293-C34 cells using TransIT-LT1 (Takara, Cat# MIR2300) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... the CPER products were transfected into HEK293-C34 cells using TransIT-LT1 (Takara, cat# MIR2300) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2019Quote: ... HEK293T and CHO-K1 cell lines were ordered from American Type Culture Collection (ATCC) and HEK293-tetON cells and CHO-tetON cells were purchased form Clontech (Takara Bio). Synthetic DNA was ordered from Twist Bioscience.
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bioRxiv - Cell Biology 2022Quote: ... Lentiviruses containing guide RNAs targeting GORAB were created in HEK293 Lenti-X cells (Takara 31966021) plated on 10 cm dishes the day before transfection ...
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bioRxiv - Bioengineering 2023Quote: HEK293 cells (JRCB Cell Bank, Osaka, Japan) and AAVpro 293T cells (Takara Bio Inc., Shiga, Japan) were cultured in Dulbecco’s modified Eagle Medium (Sigma Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... the CPER products were transfected into HEK293-hACE2/hTMPRSS2 cells by using TransIT-LT1 (Takara, Cat# MIR2305). At one day post-transfection ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 cells were transfected with pX330 vector together with an EGFP-containing plasmid (pEGFP-N1; Clontech Laboratories, Inc). Single cells were sorted using a FACS Vantage SE machine and the knockouts confirmed by sequencing ...
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bioRxiv - Neuroscience 2021Quote: ... The transfection efficiency of HEK293 cells was evaluated by transfecting cells with EGFP-N1 (Clontech; Mountain View, CA) in parallel reactions ...
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bioRxiv - Neuroscience 2022Quote: All viruses were made using the 2nd generation lentiviral packaging systems in Lenti-X HEK293 FTT cells (Takara). Lenti-X cells were passaged maximum 3 times before being used for virus production in HEK media (High glucose DMEM with 4 mM GlutaMAX ...