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Citations for Takara Bio :
1 - 50 of 4824 citations for En 1948 4 Who Dioxin Like Pcb "Starter Kit" since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... and PrimeScript™ RT Reagent Kit (RR037B, Takara Bio, Saint-Germaine-en-Laye, France), respectively ...
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bioRxiv - Cell Biology 2022Quote: ... was obtained from Takara (Saint-Germain-en-Laye, France). IR dye conjugated secondary antibodies were used at a dilution of 1:20,000 and purchased from LI-COR Biosciences (Cambridge ...
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bioRxiv - Cell Biology 2020Quote: ... was obtained from Takara (Saint-Germain-en-Laye, France). Rat HA antibody (Roche ...
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bioRxiv - Neuroscience 2022Quote: ... AP20187 (B/B) (Clontech, Saint-Germain-en-Laye France) was added 4 h post-transfection overnight.
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bioRxiv - Genomics 2020Quote: ... for subsequently cloned using the InFusion HD Cloning Kit protocol (Takara Bio, Saint-Germain-en-Laye, France). All plasmids were confirmed by Sanger sequencing ...
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bioRxiv - Microbiology 2024Quote: ... The reverse transcription was performed using the PrimeScript RT reagent kit (Takara, Saint-Germain-en-Laye, France) with a primer oligo-d(T)-AP (5’ GACCACGCGTATCGATGTCGACTTTTTTTTTTTTTTTTv 3’) ...
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bioRxiv - Genomics 2021Quote: ... Stellar competent cells (Clontech, TaKaRa, Saint-Germain-en-Laye, France) were transformed with the ligated vector and plated ...
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bioRxiv - Genomics 2021Quote: ... Stellar competent cells (Clontech, TaKaRa, Saint-Germain-en-Laye, France) were transformed with the ligated vector and plated ...
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bioRxiv - Cell Biology 2019Quote: ... Point mutants were generated using the In-Fusion HD cloning kit (TakaraBio/Clontech, Saint-Germain-en-Laye, France). DNA sequencing was carried out by LGC Genomics (Berlin ...
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The transcription factor RUNX2 drives the generation of human NK cells and promotes tissue residencybioRxiv - Immunology 2022Quote: ... RetroNectin (12 µg/mL, Takara Bio, Saint-Germain-en-Laye, France) was coated and the previously mentioned cytokines were added in combination with polybrene (8 µg/mL ...
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bioRxiv - Biochemistry 2021Quote: ... or PrimeSTAR GXL DNA Polymerase (Takara Bio, Saint-Germain-en-Laye, France) according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2022Quote: ... The Lenti-XTM HTX Packaging System (Clontech, Saint-Germain-en-Laye, France) was used with the appropriate plasmid (generated by DC Bioscience ...
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bioRxiv - Synthetic Biology 2022Quote: ... or PrimeSTAR GXL DNA Polymerase (Takara Bio, Saint-Germain-en-Laye, France) according to the manufacturers’ recommendations ...
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bioRxiv - Cell Biology 2024Quote: ... anti-GFP (JL-8, 1:3000, Clontech, Saint-Germain-en-Laye, France) and anti-Actin (AC74 ...
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bioRxiv - Immunology 2020Quote: Lenti-X™ 293T cells (Takara Bio, Clontech, Saint Germain en Laye, France) were grown using complete Dulbecco’s Modified Eagle Medium F-12 (DMEM F-12 ...
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bioRxiv - Cell Biology 2020Quote: ... Point mutants were generated by site-directed mutagenesis using the In-Fusion HD cloning kit (TakaraBio/Clontech, Saint-Germain-en-Laye, France). Sequence correctness was verified by sequencing (LGC Genomics ...
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bioRxiv - Neuroscience 2023Quote: ... Constructs containing point-mutated ARE-like sequences were prepared using the PrimeSTAR Mutagenesis Basal Kit (Takara Bio, Shiga, Japan). Because an ARE half-site can function alone to confer androgen inducibility (Pihlajamaa et al. ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was incubated with TALON resin (Takara Biotech, Saint-Germain-en-Laye, France) for 90 min in a ratio of 1 ml resin per 10 g of cells ...
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bioRxiv - Cell Biology 2023Quote: The pCAG-MDM4 expression vector was established by cloning of MDM4 sequence into a pCAG vector47 containing a multiple cloning site (pCAG-MCS) using In-Fusion® Snap Assembly Starter Bundle kit (#638945; Takara Bio). A single restriction digest was performed on the pCAG-MCS vector using XhoI (Cat ...
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bioRxiv - Plant Biology 2019Quote: ... strategy and internal primers designed from the genomic contigs (Table S1) following the kit supplier’s recommendations (Takara Bio Europe/Clontech, Saint Germain-en-Laye, France). The 3’ ends were amplified using forward internal and polyA-anchored LD primers (Table S1 ...
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bioRxiv - Biophysics 2019Quote: ... The supernatant was incubated with 2 mL TALON resin (Takara, Saint-Germain-en-Laye, France) in a tube using a rotation shaker for 30-60 minutes ...
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bioRxiv - Microbiology 2021Quote: ... and 0.7 U of Terra PCR Direct Polymerase (ClonTech Europe, Saint-Germain-en-Laye, France).
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bioRxiv - Cell Biology 2023Quote: ... All other constructs were generated by In-Fusion Cloning (Takara, Saint-Germain-en-Laye, France) using the manufacture’s instructions ...
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bioRxiv - Immunology 2022Quote: ... 0.5 µg of the isolated RNA was reverse transcribed to cDNA using an oligo dT primer and 12.5 units of reverse transcriptase mix (PrimeScript RT Reagent Kit, TaKaRa Bio, Saint-Germain-en-Laye, France). cDNA corresponding to 10 ng total RNA served as the template in a real-time PCR ...
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bioRxiv - Cell Biology 2021Quote: ... The PCR was performed with Advantage-HD DNA polymerase (Takara Bio, Saint-Germain-en-Laye, France) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: A codon-optimized synthetic gene corresponding to the full length nucleocapsid protein (Thermo GeneArt, Regensburg, Germany) was cloned into a modified pET28a vector using the In-Fusion cloning kit (Takara Bio Saint-Germain-en-Laye, France). The resulting plasmid encoded for an N-terminal His6 tag followed by thrombin and tobacco etch virus cleavage sequences upstream of the full-length nucleocapsid protein sequence ...
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bioRxiv - Genetics 2021Quote: ... and cloned into the equally digested bait DNA vector pAbAi (Takara Clontech, Saint-Germain-en-Laye, France). The yeast strain S ...
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bioRxiv - Immunology 2020Quote: ... filtered (0.45□μm) and concentrated using Lenti-XTM Concentrator (Takara Bio, Clontech, Saint Germain en Laye, France).
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bioRxiv - Cell Biology 2024Quote: ... The following antibodies were used: anti-GFP (JL-8; 1:20, Clontech, Saint-Germain-en-Laye, France), anti-PHB1 (EP2803Y ...
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bioRxiv - Microbiology 2023Quote: ... RT-qPCR was introduced to measure the relative expression of RDase genes by using the One Step PrimeScript™ RT-PCR Kit (Perfect Real Time) (Takara Bio, Saint-Germain-en-Laye, Germany). Primers targeting RDase genes were designed using the NCBI primer design tool with the setting parameters ...
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bioRxiv - Cell Biology 2021Quote: ... For iPSC-O differentiation to hepatocyte-like cells (HLC-O): Hepatic differentiation of iPSC-O was performed by Cellartis Hepatocyte Differentiation Kit (Y30050, Takara Bio, Shiga, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: ... Each half-site of the responsible ERE-like sequence was mutated into a HindIII recognition site using the PrimeSTAR Mutagenesis Basal Kit (Takara Bio, Shiga, Japan). Transcriptional activity assays with these constructs were done as described above ...
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bioRxiv - Cell Biology 2020Quote: ... we took advantage of the In-Fusion® HD Cloning system (Takara Bio Europe, Saint-Germain-en-Laye, FR) to generate a PiggyBac™ transposon system (PB ...
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bioRxiv - Immunology 2020Quote: ... Filtered viral supernatants were concentrated 100 times using Lenti-X™ Concentrator (Takara Bio, Clontech, Saint Germain en Laye, France) and resuspended in phenol red-free medium ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The recombinant Irg1-like protein was enriched using cobalt TALON Metal Affinity Resin (Takara Bio #635501) with gentle shaking for 1 hour at 4 ℃ and washed twice by centrifugation for 3 minutes at 3500 rpm at 4 ℃ and resuspension in the washing buffer (20 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2021Quote: ... 0.4 µ mol of each primer and 0.7 U of Terra PCR Direct Polymerase (ClonTech Europe, Saint-Germain-en-Laye, France).
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bioRxiv - Physiology 2022Quote: ... The insulin-like growth factor 1 (IGF1) coding sequence was PCR amplified (CloneAmp, Takara Bio; Cat. No. 639298) from cDNA generated from a rat gastrocnemius muscle ...
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bioRxiv - Cancer Biology 2022Quote: ... pooled and filtered through a 0.45 μm syringe filter and then concentrated using the Lenti-X Concentrator solution (Clontech/Takara, Saint-Germain-en-Laye, France). Intestinal WT organoids were expanded 3 days prior to infection in normal growth medium supplemented with 1 μg/ml R-Spondin ...
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bioRxiv - Immunology 2020Quote: TZM-bl (kindly provided by Dr. Quentin Sattentau, University of Oxford) and Lenti-XTM 293T cells (Takara Bio, Clontech, Saint Germain en Laye, France) were cultured in either complete DMEM or complete DMEM F-12 (Thermo Fisher ...
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bioRxiv - Evolutionary Biology 2022Quote: ... magnanima was assessed by PCR amplifying a cifB-like gene (Extended Data Table 3) in the WOwHm-t76 region with the Emerald Amp Max Master mix (TaKaRa) at 94°C for 3 min ...
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bioRxiv - Plant Biology 2019Quote: The full-length cDNA of NaERF2-like with two HA flags was cloned into pCAMBIA1301 vector after 35S promoter via in-fusion technique (Clontech). N ...
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bioRxiv - Molecular Biology 2023Quote: ... flanking the site of alterations (HDE-like motifs) were amplified from the genomic DNA using CloneAmp HiFi PCR Premix (Clontech), A-tailed with DreamTaq DNA polymerase and ligated into pGEM-T Easy vector ...
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bioRxiv - Cancer Biology 2022Quote: ... pooled and filtered through a 0.45 μm syringe filter and then concentrated using the Lenti-X Concentrator solution (Clontech/Takara, Saint-Germain-en-Laye, France). Intestinal WT organoids were expanded 3 days prior to infection in normal growth medium supplemented with 1 μg/ml R-Spondin ...
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bioRxiv - Biochemistry 2024Quote: ... a two-round PCR strategy was required to amplify the ORF using a high-fidelity PrimeStar ® DNA polymerase (Takara Europe, Saint-Germain-en-Laye, France). The first round of the nested PCR was carried out using the above cDNA as the template with primers designed in the 5’- and 3’-untranslated regions (UTR ...
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bioRxiv - Immunology 2021Quote: ... Additional to the NF-κB binding motifs NF-κB response element and a TATA-like sequence from pNFκB-Luc vector (Clontech Cat. No. 631904) were added to the Firefly luciferase gene and introduced into pCDH-CMV-EF1-Puro.
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bioRxiv - Cell Biology 2020Quote: ... we designed primers with complementary overhangs (Supplementary Table S3) and used them to generate a “NEON insert” by PCR amplification (high-fidelity DNA polymerase, CloneAmp™, Takara Bio Europe, Saint-Germain-en-Laye, FR) of the mNeonGreen sequence (Allele Biotechnology and Pharmaceuticals Inc. ...
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bioRxiv - Neuroscience 2022Quote: ... RNA with RIN > 7 was prepared for sequencing using the RNAseq Ovation V2 kit (Nugen) or Smart-seq 4 low-abundance RNA kit (Takara) and sequenced on the Illumina HiSeq 4000 or Novaseq 2 platform in collaboration with Oxford Genomics Centre.
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bioRxiv - Molecular Biology 2022Quote: ... were used to prepare RNA-Seq libraries using the SMART-Seq v.4 Kit (Clontech) as previously described42 ...
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bioRxiv - Immunology 2023Quote: ... RNA was then amplified using SMART-Seq v.4 Ultra Low Input RNA kit (Clontech, cat. 63488). Subsequently ...
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bioRxiv - Immunology 2019Quote: ... using the manufacturers protocol and amplified using SMARTer Ultra Low Input RNA kit for sequencing (version 4, Clontech). Sequencing libraries were generated using TruSeq Nano DNA Sample Preparation kits (Illumina) ...