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Citations for Takara Bio :
1 - 50 of 711 citations for Dichlorine bis pentane 2 4 dionato o o' platinum since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... PK-15 cells were transfected with PX459-Ifnar1-k/o or PX459-Stat2-k/o plasmids using the TransIT-X2 Dynamic Delivery System (TaKaRa, Cat# V6100) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... For iPSC-O differentiation to hepatocyte-like cells (HLC-O): Hepatic differentiation of iPSC-O was performed by Cellartis Hepatocyte Differentiation Kit (Y30050, Takara Bio, Shiga, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... They were then incubated O/N at 4°C with the corresponding primary antibody: anti-DsRed (1:500; Clontech), mouse anti-GFP ([1:100] ...
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bioRxiv - Plant Biology 2021Quote: ... SD growth medium w/o Ade−His−Leu−Trp− (Himedia) and supplements media (Clontech) were used for the Y2H experiment.
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bioRxiv - Genomics 2021Quote: ... DNA-barcoded lectin was dialyzed by Tube-O-DIALYZER (Takara Bio Inc., Shiga, Japan) and concentrated using centrifugal filters having a 10 kDa molecular weight cut off (MWCO ...
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bioRxiv - Cell Biology 2021Quote: RNAs were extracted from A498 and 786-O cells using the TRIzol reagent (Takara, Otsu, Japan) and then reverse transcribed into cDNA using the TaqMan™ advanced miRNA cDNA synthesis kit (Thermo Fisher) ...
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bioRxiv - Cell Biology 2023Quote: ... The β-galactosidase activity was determined by ONPG (o-nitrophenyl β-D-galactopyranoside) liquid assay (quantitative) as mentioned elsewhere (Clontech Laboratories, 2008). β-galactosidase activity from each sample was calculated from at least three biological replicates and the concentration of cells (O.D.600 ...
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bioRxiv - Biochemistry 2022Quote: ... and ligated into the multiple cloning site 2 (MCS2) of the pTRE3G-BI vector (Clontech, Mountain View, CA, USA), resulting in the construct pTRE3G-BI/GPR83-LgBiT ...
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bioRxiv - Plant Biology 2024Quote: ... Samples were then run on a 4-12% Bis-Tris gel and transferred to a membrane for western blotting using α-GFP (Clontech) and α-HA (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2022Quote: ... modified from pTRE-Tight-BI (Takara-Bio) (Koraichi et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... and subcloned into pTRE3G-BI (TaKaRa Bio) to construct pTRE3G-BI-GFP ...
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bioRxiv - Cell Biology 2020Quote: ... Protein samples were run on a 4-12% Bis-Tris gel (Novex) and Western blots were performed with the following antibodies: α-GFP (Clontech Living Colors 632381 (JL-8); RRID:AB_2313808 ...
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bioRxiv - Synthetic Biology 2023Quote: ... pTRE3G and rtTA3G sequences were amplified from pTRE3G-BI-ZsGreen1 (Takara #631339) and pCMV-Tet3G Vector (Takara # 631335) ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant was clarified by centrifugation (292,055 g, 60 min, 4°C) and bound to 2 ml of TALON IMAC resin (Clontech) overnight with 10 rpm rotation in the presence of 20 mM imidazole and NaCl added up to 800 mM ...
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bioRxiv - Molecular Biology 2021Quote: ... Pre-miR-122 or pre-miR-146a sequence cloned within pTRE-Tight-BI Vector (Clontech) in NheI and NotI sites or Bam HI and Hind III to make induced miR-122 (imiR-122 ...
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bioRxiv - Immunology 2023Quote: ... a derivative of pInducer20-NA with the bidirectional doxycycline-inducible promoter from pTRE3G-BI (TaKaRa). Expression of VHH-HA and the C1C-EGFP inflammasome reporter was thus doxycycline-inducible ...
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bioRxiv - Immunology 2024Quote: ... T cells were activated for 2 days and NK cells were activated for 4 days followed by retronectin (Takara, T100B) mediated viral transduction ...
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bioRxiv - Cancer Biology 2022Quote: ... 4×106 B cells per well were plated in 2 mL on 6-well plates that had been coated with RetroNectin (25 μg/mL, 4°C, overnight; #T100B, Takara), blocked with 2% BSA in PBS (1h ...
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bioRxiv - Biochemistry 2022Quote: The coexpression construct pTRE3G-BI/GPR83-LgBiT:SmBiT-ARRB2 and the expression control vector pCMV-TRE3G (Clontech) were cotransfected into HEK293T cells using the transfection reagent Lipo8000 (Beyotime Technology) ...
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bioRxiv - Neuroscience 2022Quote: ... protein lysate was immunoprecipitated for 2 h at 4°C with the following antibodies: mouse anti-GFP (1:1000; Takara Bio), mouse anti-FLAG (1:1000 ...
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bioRxiv - Immunology 2023Quote: ... The mixture was then chilled on ice for 2 min and then mixed with 4 μl 5x first-strand buffer (Takara Bio), 2 μl 100 mM DTT ...
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bioRxiv - Cell Biology 2020Quote: ... for 1 h at 4 °C with rotation and then transferred to gravity columns (Talon® 2 ml Disposable Gravity Column, Clontech, #635606-CLI). The lysate was drained and the beads were washed five times with cold wash buffer (50 mM Tris ...
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bioRxiv - Genetics 2022Quote: ... reverse transcription was carried out using 2 μL of RNA mixed with 4 μL of 5×PrimeScript IV cDNA Synthesis Mix (Takara, Code No. 6215A) containing PrimeScript IV RTase ...
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bioRxiv - Microbiology 2024Quote: ... and was then incubated with 2 mL TBS pH 8.0 pre-equilibrated Talon® Metal Affinity resin during 2 h at 4°C in a vertical rotating mixer (Takara Bio, Kusatsu, Japan). The resin was washed with 10 volumes of TBS pH 8.0 buffer containing 0.1% Triton X-100 and 10 mM imidazol (both from Sigma) ...
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bioRxiv - Synthetic Biology 2023Quote: ... 2 µL of 2 mM dNTPs (Takara #4025), and 0.6 µL of 100% DMSO (NEB #12611P) ...
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bioRxiv - Synthetic Biology 2023Quote: ... 2 µL of 2 mM dNTPs (Takara #4025), and 0.6 µL of 100% DMSO (NEB #12611P) ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 (TaKaRa) and purified using ProbeQuant G-50 Micro Columns (GE Healthcare ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 (TaKaRa) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... A cDNA coding for 128QHTT with C-terminal fusion to a FLAG-His affinity tag was cloned into the vector pTRE-tight-BI-AcGFP1 (Clontech) for expression of 128QHTT upon induction with Dox ...
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bioRxiv - Cell Biology 2021Quote: ... WT TARPs and ncAA-tagged TARPs were subcloned into a bidirectional doxycycline-inducible expression vector pTRE3G-BI (Takara Bio, #631332) using the restriction sites KpnI/XbaI ...
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bioRxiv - Biochemistry 2022Quote: The Tet-On inducible expression construct pTRE3G-BI/GPR83-EGFP or pTRE3G-BI/GPR83-IRES-EGFP was transiently transfected into HEK293T cells together with the expression control vector pCMV-TRE3G (Clontech) using the transfection reagent Lipo8000 (Beyotime Technology) ...
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bioRxiv - Neuroscience 2022Quote: ... Stable cell line were generated as follows: A bicistronic expression vector was generated in the pTRE-tight-BI plasmid (Clontech) containing both human Nptx2 and human Nptxr cDNAs ...
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bioRxiv - Immunology 2022Quote: ... or MAP2 kinases under a bidirectional dox-inducible promoter were generated using lentiviral vector pInducer20bi-NA, a derivative of pInducer20-NA (Schmidt et al., 2016a) using the promoter from pTRE3G-BI (Takara Bio).
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bioRxiv - Cancer Biology 2023Quote: ... pBH001 is comprised of a tetracycline-regulated bidirectional promoter for inducible expression of both firefly luciferase (a fragment cloned from the pTRE3G-BI-Luc control plasmid (Takara Bio)) and a recoded human L1 sequence (encoding both ORF1 and ORF2 ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 kit (Takara) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 (Takara, #6045) according to manufacturer’s protocols ...
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bioRxiv - Immunology 2022Quote: ... version 2 (Takara). Fastq files were generated from bcl files using BCL2FASTQ v2.17.1.14 with default parameters ...
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bioRxiv - Neuroscience 2022Quote: ... 2 (Takara Bio).
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bioRxiv - Molecular Biology 2023Quote: ... 2 (Takara Bio).
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bioRxiv - Cell Biology 2022Quote: ... SEPT2 constructs in pTRIP TRE Bi plasmids and all interface mutants in pTRIP TRE Bi plasmids were cloned using seamless cloning (In-Fusion HD Cloning Plus Kit from Takara Bio, 638910). All pcDNAs and all wild-type SEPT7 and SEPT9-containing constructs in pTRIP TRE Bi plasmids were generated with classical cloning ...
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bioRxiv - Neuroscience 2020Quote: ... 4 U RNAse inhibitor (Takara, 2313A), 10mM dNTPs (Thermo Scientific ...
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bioRxiv - Biochemistry 2024Quote: HEK293T cells were transiently cotransfected with the expression construct pTRE3G-BI/S1PR2-LgBiT:SmBiT-ARRB2 and the expression control vector pCMV-TRE3G (Clontech, Mountain View, CA, USA) using the transfection reagent Lipo8000 (Beyotime Technology ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 (Takara, Shiga, Japan) and 0.32 µM of each primer according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... 4 ul 2.5 μM dNTP mixture (Takara), 0.8 ul 100mM DTT and 14.2 ul RNase-free water ...
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bioRxiv - Microbiology 2022Quote: ... and 4 μM random hexamer primers (Takara Bio ...
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bioRxiv - Microbiology 2020Quote: ... and 4) TaKaRa LA Taq (TaKaRa RR042). For each library ...
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bioRxiv - Microbiology 2024Quote: ... PrimeScript reverse transcriptase (4 µl) (Takara, #2680B), and filtered water (6 µl ...
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bioRxiv - Neuroscience 2022Quote: ... Doxycycline (2 mg/L, Clontech) was also included on d0 to induce TetO gene expression by binding to rtTA and the TetO promoter upstream of the Ngn2 gene ...
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bioRxiv - Neuroscience 2023Quote: ... 2 µg/ml doxycycline (Takara) was added on day 0 to induce TetO gene expression ...
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bioRxiv - Cell Biology 2024Quote: ... Ver.2 (Takara, cat# 6233), as per manufacturer’s protocol.