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Citations for Takara Bio :
151 - 200 of 1054 citations for Dengue Virus Serotype 2 Envelope Protein HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Virus titer was determined by using Jurkat T cells and Lenti-X GoStix Plus (Takara Clontech).
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bioRxiv - Microbiology 2023Quote: ... RNA was purified from the medium with NucleoSpin® RNA Virus kit (U0956A, Takara Bio Inc.) by following the manufacture’s instruction and subjected to RT-qPCR with the primer pairs for EGFP (5′-CAAGCTGACCCTGAAGTTCATCTG-3′ and 5′-TTGAAGAAGTCGTGCTGCTTCATG-3′ ...
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bioRxiv - Plant Biology 2023Quote: ... under the control of cauliflower mosaic virus 35S promoter in the binary vector pBI121 (Clontech, USA) was transferred into Agrobacterium tumefaciens strain GV3101 and used to transform Arabidopsis by floral dip method and tobacco plants by leaf disk method (Horsch et al. ...
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bioRxiv - Microbiology 2021Quote: ... 10 μg of Spike expressor and 2 μg of a green fluorescent protein (GFP) expressor (pIRES2-eGFP; Clontech) was transfected into 2 × 106 293T cells ...
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bioRxiv - Molecular Biology 2021Quote: TET-ON stable HEK293 cells with the Tetracycline-inducible expression constructs were grown in DMEM supplemented with 10% TET-approved FCS (Clontech) and induction of expression of respective gene product was carried out for indicated time durations using 400 ng/ml Doxycycline (SIGMA) ...
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bioRxiv - Neuroscience 2020Quote: We transiently co-transfected cDNA constructs of GluN1 and GluN2A into mammalian human embryonic kidney 293 (HEK293) with a separate pEGFP-Cl vector (Clontech) at a ratio of 4.5:4.5:1 (GluN1/GluN2A/EGFP ...
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bioRxiv - Cancer Biology 2023Quote: ... His-TEV tagged protein was captured with Co-TALON resin (Clonetech, Takara Bio USA, 2 mL slurry/liter culture) at 4 ºC for 1 h with constant end-to-end mixing ...
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bioRxiv - Microbiology 2023Quote: ... The protein-bead mixtures were then loaded onto a gravity column (TALON 2 mL Disposable Gravity Column, Takara #635606). Lysate was loaded into the column 5 times with gravity flow and then washed in a 1M NaCl buffer 3 times ...
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bioRxiv - Genomics 2020Quote: ... The titer of each virus was determined using the Lenti-X Go-Stix Plus kit (Takara Bio), according to the manufacturer’s instruction.
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bioRxiv - Microbiology 2019Quote: ... Whole genomic amplification of the influenza virus was conducted using Ex Taq™ Hot Start Version (TaKaRa). Forward primers were Uni-1.5 and Uni-0.5 mixed in a ratio of 3:2 ...
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bioRxiv - Synthetic Biology 2022Quote: Pantropic vesicular stomatitis virus G pseudotyped lentivirus was produced via transfection of LentiX 293T cells (Clontech #11131D) with a pHR’SIN:CSW transgene expression vector and the viral packaging plasmids pCMVdR8.91 and pMD2.G using FuGENE HD (Promega ...
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bioRxiv - Microbiology 2022Quote: ... LASVpp-BlaM virus was concentrated 10 times with Lenti-X concentrator (Clontech Laboratories, Mountain View, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... and 5 units of SMART Moloney murine leukemia virus reverse transcriptase (Takara Bio, Mountain View, CA, USA). The RT reaction was carried out at 42°C for 2 hrs ...
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bioRxiv - Cell Biology 2019Quote: Virus titer was determined by quantitative Polymerase Chain Reaction (qPCR) using Adeno-X qPCR Titration Kit (Clontech) on an Applied Biosystems 7900HT.
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bioRxiv - Immunology 2020Quote: ... and total RNA was reverse-transcribed to cDNA with Moloney Murine Leukemia Virus Reverse Transcriptase (2641B, TAKARA). Real-time quantitative PCR (qPCR ...
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bioRxiv - Microbiology 2020Quote: ... The purified virus was titrated with Lenti-X p24 Rapid Titer Assay (Takara Bio, Cat. No. 632200). The virus was stored at -80 °C for future use.
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bioRxiv - Cell Biology 2021Quote: ... cDNAs were synthesized from total RNA using Moloney Murine Leukemia Virus (M-MLV) Reverse Transcriptase (Takara, Japan) based on the manufacturer’s instructions.
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bioRxiv - Pathology 2020Quote: ... and 5 units of SMART Moloney murine leukemia virus reverse transcriptase (Takara Bio, Mountain View, CA, USA). The RT reaction was carried out at 42°C for 2 hrs ...
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bioRxiv - Cell Biology 2020Quote: ... and virus was collected 72 and 96 hours after transfection and concentrated using Lenti-X concentrator (Clontech). Cells were then transduced with virus after growing to 75% confluency and supplemented with 10μg/mL polybrene ...
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bioRxiv - Cell Biology 2022Quote: The AAV-sgApc virus was produced from the pAAV-Guide-it-Down construct (Clontech Laboratories Inc., 041315) using assembly primers:
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bioRxiv - Cancer Biology 2023Quote: ... The virus titer was determined using the Lenti-X™ p24 Rapid Titer Kit (Cat: 632200, Clontech).
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bioRxiv - Microbiology 2023Quote: The plasmid constructs used as repair template for recombinant virus generation were generated using InFusion cloning (TaKaRa). The sequence references below are based on the HSV-1 KOS genome accession number JQ673480 (Macdonald et al 2012) ...
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bioRxiv - Neuroscience 2022Quote: AAVs were packaged via calcium phosphate transfection of HEK293 cells as detailed in (McClure et al 2011) and purified using an AAVpro purification kit (Takara Bio).
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bioRxiv - Microbiology 2023Quote: ... HEK293 cells were cotransfected with the S expression plasmids (400 ng) and pDSP 61,62 (400ng) using TransIT-LT1 (Takara, Cat# MIR2306). On day 3 (24 hours post transfection) ...
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bioRxiv - Microbiology 2021Quote: 293T were transfected with full length SARS-CoV-2 Spikes and a green fluorescent protein (GFP) expressor (pIRES2-eGFP; Clontech) using the calcium-phosphate method ...
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bioRxiv - Microbiology 2022Quote: ... PreS2-LHBS sequence (deletion nucleotides 2-55) was cloned into the protein stability construct after the c-terminal of EGFP by In-Fusion cloning (Takara). The protein stability construct was a kind gift from Dr ...
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bioRxiv - Physiology 2022Quote: ... the beads were well-washed by column binding buffer 5 times and then incubated in 2× Protein SDS PAGE Loading (Takara) 100°C for 5 min to completely elute the proteins ...
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bioRxiv - Pathology 2021Quote: ... Expression of the GST-N protein of SARS-CoV-2 was induced by isopropyl-D-1-thiogalactopyranoside (0.3 mM IPTG, Takara Bio). The cell pellets were sonicated ...
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bioRxiv - Microbiology 2022Quote: ... and E484Q mutations of the SARS-CoV-2 spike protein were determined by qPCR using mutation detection kits purchased from Takara Bio (Shiga ...
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bioRxiv - Cancer Biology 2021Quote: ... The collected media containing virus was filtered through 0.45μm pore filter and mixed with Lenti-X concentrator (Clontech) as indicated by manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2019Quote: ... The vaccinia virus DNA polymerase was used to clone the protospacers into a lentiviral construct (In-fusion, Takara). The lentiviral construct was linearized by Bfua1 digestion (New England Biolabs) ...
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bioRxiv - Bioengineering 2021Quote: ... The virus titre was calculated using the ELISA-based Lenti-X™ p24 Rapid Titer Kit (Takara Bio) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... Cells expressing lentiviral vectors were created by following the manufacturer’s instructions for virus preparation and cell infection (Clontech). Cells were selected for expression by treatment with puromycin ...
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bioRxiv - Cell Biology 2022Quote: ... Cells expressing lentiviral vectors were created by following the manufacturer’s instructions for virus preparation and cell infection (Clontech). Cells were selected for expression by treatment with puromycin ...
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bioRxiv - Cell Biology 2023Quote: ... The viral supernatants were collected 72 hr after transfection followed by virus concentration using Lenti-X concentrator (TaKaRa). Virus containing media was added to ATG3 KO HeLa cells with 8 μg/mL polybrene (Sigma-Aldrich ...
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bioRxiv - Biophysics 2023Quote: ... The virus of each construct was produced in Lenti-X™ 293 T Cell Line (Takara, Cat. #632180) and previously described74 ...
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bioRxiv - Physiology 2024Quote: ... Three volumes of concentrated virus medium were subsequently mixed with one volume of Lenti-X concentrator (Takara Bio) and rotated at 4°C overnight before centrifugation (1500 g for 45 min at 4°C) ...
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bioRxiv - Cell Biology 2023Quote: ... Virus containing media was collected from the LentiX-293T cells and concentrated using Lenti-X Concentrator (Takara Bio). pLVX-Tet3G virus and polybrene was added to L6-myoblast cells and cells were positively selected using neomycin to create Tet3G expressing cells ...
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bioRxiv - Microbiology 2022Quote: ... HEK293 cells were cotransfected with the S expression plasmids (400 ng) and pDSP8-11 (400 ng) using TransIT-LT1 (Takara, Cat# MIR2300). To prepare target cells ...
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bioRxiv - Systems Biology 2020Quote: ... proteins in whole cell extract and immunoprecipitated fractions were digested with 2 mg/mL proteinase K (Takara Bio Inc., Kusatsu, Japan) at 42°C for 2 h ...
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bioRxiv - Biochemistry 2022Quote: ... cells were transfected with plasmids expressing either SARS-COV-2 S protein WT or mutants (E1182K, L1193G, E1182K/L1193G, L1182K/L1186G/L1193G) by using Calcium phosphate transfection kit (Takara-Bio). 24 h post transfection ...
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bioRxiv - Neuroscience 2022Quote: ... protein lysate was immunoprecipitated for 2 h at 4°C with the following antibodies: mouse anti-GFP (1:1000; Takara Bio), mouse anti-FLAG (1:1000 ...
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bioRxiv - Molecular Biology 2024Quote: ... the U2OS 2-6-3 cells expressing degron-tagged LacI fusion proteins were incubated with 300 nM Shield1 ligand (Takara Bio) and 1 µg/mL doxycycline for 24 hours ...
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bioRxiv - Molecular Biology 2020Quote: ... JEG3 cells (human; sex: female, placenta epithelial), HEK293 derivative Lenti-X™ 293T cells (human; sex: female, kidney epithelial) obtained from Takara (cat. 632180), Huh-7.5 heptoma cells (human ...
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bioRxiv - Cell Biology 2019Quote: ... Virus was produced by transfection of 293FT cells with pBABE- vimentin and pCL-Eco using Xfect transfection reagent (Clontech) and collection of supernatants 48 and 72hrs post transfection ...
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bioRxiv - Immunology 2021Quote: ... 48hrs later the virus-containing supernatant from HEK-293T cells was concentrated 100-fold using Lenti-X concentrator (Takara). Titration was performed on HEK293T cells (ATCC ...
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bioRxiv - Cell Biology 2021Quote: ... Virus is produced by transfection of 293FT cells with pBABE-vimentin and pCL-Eco using Xfect transfection reagent (Clontech) and collection of supernatants 48 and 72 hours post transfection ...
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bioRxiv - Cell Biology 2021Quote: ... after which virus-containing supernatants were harvested and concentrated approximately 40-fold using Lenti-X Concentrator (Takara Bio, 631232) per the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... bone marrow was harvested and transduced with SINV virus on plates coated with 10 μg/cm2 Retronectin (T100, Takara) for 1.5-2 hrs at 650×g and 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... Virus was resuspended in cold PBS and titre was determined using Lenti-X p24 Rapid Titre Kit (Takara Bio) according to manufacturer’s instructions.