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Citations for Takara Bio :
51 - 100 of 546 citations for Cytomegalovirus Purified Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2019Quote: ... cells bearing the corresponding plasmids and were purified according to.63 Partially purified lysates were loaded onto equilibrated cobalt His-TALON columns (Clontech, Mountain View, CA). The column was washed with 40 to 50 volumes of wash buffer (50 mM NaH2PO4 ...
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bioRxiv - Microbiology 2020Quote: ... iRBDs were purified by passage over Co-NTA agarose (Clontech) followed by gel filtration chromatography on Superdex 200 (GE Healthcare ...
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bioRxiv - Plant Biology 2020Quote: ... the proteins were purified by TALON Metal Affinity Resin (Clontech) and filtered with Amicon Ultra 0.5ml (30K ...
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bioRxiv - Microbiology 2020Quote: ... Clarified supernatants were purified in batch over Talon resin (Takara) prior to buffer exchanging into 20mM Tris pH8 150mM NaCl and flash freezing.
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bioRxiv - Cell Biology 2020Quote: ... The solubilized protein was purified by Talon-resin (Clontech/Takara) using the hexa-histidine-tag fused at the N-terminus of the protein ...
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bioRxiv - Cell Biology 2020Quote: ... The solubilized protein was purified by Talon-resin (Clontech/Takara) using the hexa-histidine-tag fused at the N-terminus of the protein ...
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Biosynthesis of Circular RNA ciRS-7/CDR1as Is Mediated by Mammalian-Wide Interspersed Repeats (MIRs)bioRxiv - Molecular Biology 2019Quote: ... Purified RNA was reverse-transcribed using PrimeScript II (Takara Bio) with oligo-dT and random primers ...
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bioRxiv - Microbiology 2019Quote: ... The resulting enzyme was then purified by Talon chromatography (Clontech) and underwent iron-sulfur cluster reconstitution using previously published protocols 26,30,31.
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bioRxiv - Cell Biology 2019Quote: ... Protein was purified on a His60 Ni Superflow column (Clontech) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... The solubilized fragment was purified by Talon-resin (Clontech/Takara) using the hexa-histidine-tag fused at the N-terminus of the fragment ...
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bioRxiv - Cell Biology 2019Quote: ... The solubilized fragment was purified by Talon-resin (Clontech/Takara) using the hexa-histidine-tag fused at the N-terminus of the fragment ...
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bioRxiv - Molecular Biology 2020Quote: ... but purified using TALON cobalt metal affinity resin (Takara Bio) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2019Quote: ... The lentivirus was purified by Lenti-XTM Concentrator (Clontech, 631231) and tittered by qPCR Lentivirus Titration Kit (Applied Biological Materials Inc. ...
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bioRxiv - Immunology 2020Quote: ... and purified over IMAC resin (HisTALON™ Superflow Cartridge, TAKARA), using a fast protein liquid chromatography system (ÄKTA FPLC Systems ...
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bioRxiv - Immunology 2020Quote: ... Fabs were purified by passage over Co-NTA agarose (Clontech) followed by gel filtration chromatography on Superdex 200 (GE Healthcare ...
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bioRxiv - Immunology 2020Quote: ... Fabs were purified by passage over Co-NTA agarose (Clontech) followed by gel filtration chromatography on Superdex 200 (GE Healthcare ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR products were pooled by template and purified (Nucleospin, Takara). RACE amplicons were end repaired ...
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bioRxiv - Molecular Biology 2022Quote: ... Purified DNA was directly ligated with pMD18-T vector (Takara) for TA cloning according to the pMD18-T kit protocol ...
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bioRxiv - Systems Biology 2022Quote: ... viruses were purified using the Retro-X Concentrator kit (Clontech) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... purified using NucleoSpin Gel and PCR Clean-Up Kit (Takara), cloned into pRACE vector (provided in the kit ...
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bioRxiv - Biochemistry 2023Quote: ... protein complexes were purified from by Ni2+-NTA (Takara Bio) affinity chromatography (Dsl1:Qb:Qc and His7-Tip20:Sec20 ...
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bioRxiv - Microbiology 2023Quote: ... Fabs were purified by passage over Co-NTA agarose (Clontech) followed by gel filtration chromatography on Superdex 200 (GE Healthcare ...
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bioRxiv - Cancer Biology 2023Quote: ... Viral preparations were then purified by LentiX concentrator (Takara Bio). GSCs were infected with lentivirus and after 24 hours of incubation at 37°C ...
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bioRxiv - Immunology 2024Quote: ... His-tagged CD45RO was purified by TALON affinity resin (Takara) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... AAV was purified with the AAVpro Purification Kit (Takara, 6666) according to manufacturer’s instruction ...
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bioRxiv - Microbiology 2020Quote: ... The amplicons were purified by DNA Amplification Clean Up Kit (Clontech). Amplicons were pooled in equimolar ratios prior to library preparation ...
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bioRxiv - Microbiology 2020Quote: ... HIV viral particles were purified using the Lenti-X concentrator (Takara) and were resuspended in fresh DMEM medium supplemented with 5% of FBS ...
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bioRxiv - Microbiology 2019Quote: ... the viral RNA was purified by NucleoSpin® RNA Kit (TAKARA). Recombinant INHis proteins (300 nM ...
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bioRxiv - Biochemistry 2020Quote: ... CST was purified from the clarified lysate using Talon resin (Clontech) followed by protease cleavage of the GFP-His10 tag and finished with size exclusion chromatography on a Superdex 200 column equilibrated in Buffer A (25 mM HEPES pH 7.5 ...
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bioRxiv - Neuroscience 2021Quote: ... coli BL21 and purified on 5 mL Talon column (Clontech®) loaded with Cobalt ...
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bioRxiv - Immunology 2022Quote: ... BAC DNA was purified using the NucleoBond BAC 100 kit (Takara). Viral DNA was isolated from viral stocks by digestion with proteinase K (Roche ...
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bioRxiv - Biophysics 2020Quote: ... The complex was then purified by binding on Talon resin (Clontech) and eluted in 150 mM imidazole ...
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bioRxiv - Neuroscience 2021Quote: ... His-tagged Cbln1 were purified by Talon metal affinity resin (Clontech) and dialyzed against HBSS ...
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bioRxiv - Genomics 2019Quote: ... Purified PCR products were cloned into pMD18-T vector (TaKaRa, Japan) for transformation to DH5-α competence cells of Escherichia coli and sequencing.
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bioRxiv - Developmental Biology 2019Quote: ... The BACs were purified with the nucleobond BAC 100 kit (Clontech). We co-injected 1 nl of 40 ng/μl Tol2 mRNA and 50–250 ng/μl of the cdh1:cdh1-sfGFP or cdh1:cdh1-TagRFP BAC transgene DNA into the lifting cell of the zygote of 0 to 20-minute old embryos ...
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bioRxiv - Immunology 2020Quote: ... Clarified supernatants were purified using a 5mL Cobalt affinity column (Takara). Purified protein was filtered or concentrated and flash frozen in a buffer containing 50 mM Tris pH 8.0 and 150 mM NaCl prior to cryoEM analysis ...
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bioRxiv - Microbiology 2020Quote: ... Modified BACs were purified using a Nucleobond BAC 100 kit (Clontech). BAC quality was assessed by digestion with RsrII and SbfI (New England Biolabs) ...
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bioRxiv - Cell Biology 2021Quote: ... purified nucleic acids were treated with DNA enzyme I (Takara, Japan). RNA was reverse transcribed using RevertAid reverse transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The purified RNAs were reverse-transcribed using PrimeScript reverse transcriptase (Takara) and primer 3 and then PCR-amplified using primer 3 and 4 ...
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bioRxiv - Developmental Biology 2022Quote: ... The final BAC was purified with Nucleobond BAC 100 kit (Clontech) and co-injected with I-Sce I into one-cell-stage zebrafish embryos ...
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bioRxiv - Biochemistry 2022Quote: ... The proteins were purified using TALON Metal Affinity Resin (Takara Bio) and eluted with a buffer containing 200 mM imidazole ...
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bioRxiv - Developmental Biology 2022Quote: ... The final BAC was purified with Nucleobond BAC 100 kit (Clontech) and co-injected with 50 ng/μl Tol2 RNA into one-cell-stage zebrafish embryos ...
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bioRxiv - Genetics 2022Quote: ... and then purified with RNase-Free DNase I (Takara Company, China). 1% agarose gel was used to evaluate the RNA contamination and degradation ...
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bioRxiv - Neuroscience 2023Quote: ... were purified with the NucleoBond Xtra Midi EF kit (Takara 740420). Il6ra knockdown efficiency was assessed by transient transfection of sgRNA and dCas9-KRAB-MeCP2 into the mHypoA hypothalamic neural cell line (Cedarlane Labs ...
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bioRxiv - Immunology 2022Quote: ... Clarified culture supernatants were purified over Cobalt-TALON affinity resin (Takara), with subsequent purification via size exclusion chromatography (SEC ...
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bioRxiv - Microbiology 2023Quote: ... Nanobodies were purified from clarified supernatants using Talon affinity resin (Takara). Eluted nanobodies were concentrated in an Amicon centrifugal spin concentrator (3K MWCO) ...
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bioRxiv - Biochemistry 2023Quote: ... The protein was purified by metal-affinity chromatography (Talon resin, Clontech), the affinity tag removed using tobacco etch virus (TEV ...
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bioRxiv - Cell Biology 2023Quote: ... His6-tagged proteins were purified with Talon metal affinity resin (Clontech) using the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... The resulting amplicons were PCR-purified (NucleoSpin® Extract II, Clontech). To clone the pool of oligonucleotides in the lentiCRISPRv2 vector ...
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bioRxiv - Cell Biology 2023Quote: ... gel-purified (NucleoSpin Gel and PCR Clean-up kit from Takara) and the purified fragment was stored at -20°C ...