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Citations for Takara Bio :
1 - 50 of 101 citations for Creatine Kinase M Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... and T4 polynucleotide kinase (Takara). The extension reaction was performed with the AccuPower® DNA Sequencing Kit (Bioneer) ...
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bioRxiv - Cell Biology 2022Quote: ... phosphorylated by T4 polynucleotide kinase (TaKaRa) and inserted between SpeI and NotI restriction sites (pFastBac1-hCAP-D3-[3C]-StrepII ...
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bioRxiv - Cell Biology 2022Quote: ... phosphorylated by T4 polynucleotide kinase (TaKaRa), and inserted between BamHI and EcoRI restriction sites by using restriction enzymes and a DNA ligation kit (TaKaRa) ...
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bioRxiv - Genomics 2019Quote: ... together with phosphorylated (T4 polynucleotide kinase, Takara) primers that spanned the length of the RNA of the retrozyme (i.e. ...
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bioRxiv - Cell Biology 2021Quote: ... and then phosphorylated by T4 polynucleotide kinase (Takara, 2021S). pX459 was digested with BbsI ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by conducting a T4 polynucleotide kinase reaction (TAKARA). After phosphorylation ...
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bioRxiv - Genetics 2020Quote: ... 5′-phosphorylated by T4 polynucleotide kinase (TaKaRa, Shiga, Japan), self-ligated by T4 ligase (TaKaRa ...
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bioRxiv - Plant Biology 2023Quote: ... and end-labeled by T4 polynucleotide kinase (Takara Bio) and [γ-32P]-ATP (PerkinElmer) ...
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bioRxiv - Plant Biology 2021Quote: ... Strands with a 5′ monophosphate were radiolabeled with T4 polynucleotide kinase (Takara) and [γ-32P]ATP ...
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bioRxiv - Microbiology 2019Quote: ... and a Renilla luciferase driven by the thymidine kinase promoter (pRLTK; Clontech) was performed according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... a stepwise RNA end repair was carried out using T4 polynucleotide kinase (TaKaRa). A 3′-dephosphorylation and 5′-phosphorylation reaction was performed using T4 PNK enzyme (TaKaRa) ...
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bioRxiv - Biochemistry 2021Quote: ... T4 polynucleotide kinase and TransIT®-LT1 Reagent were purchased from TAKARA (Shiga, Japan). SB203580 was purchased from Adipogen Life Science (San Diego ...
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bioRxiv - Immunology 2023Quote: ... Plates were coated with 5μg/mL of target peptide using coating reagent from the Takara Peptide Coating Kit (Takara cat. #MK100). Measles peptide was utilized as a negative control ...
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bioRxiv - Cell Biology 2023Quote: ... and the 5′-end was phosphorylated with T4 polynucleotide kinase (Cat. # 2021S: Takara, Kyoto, Japan). This phosphorylated DNA fragment was ligated to the Bbs I cloning site in pSpCas9(BB)-2A-Puro (PX459 ...
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Mck1 defines a key S-phase checkpoint effector in response to various degrees of replication threatsbioRxiv - Molecular Biology 2019Quote: ... DNA was transferred to HyBond N+ in transfer buffer (0.4 M NaOH, 1 M NaCl) and UV cross-linked before hybridization with a random primed probe (Takara) overnight at 42°C and washed twice for 20 min with 0.5× SSC 0.1% SDS at 65°C.
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bioRxiv - Microbiology 2023Quote: ... corresponding to these sgRNA sequences (LpAsp_For and LpAsp_Rev as well as LpCht_For and LpCht_Rev) were phosphorylated by T4 polynucleotide kinase (TAKARA) followed by annealing and cloning into BbsI digested pSPneogRNAH vector (Zhang and Matlashewski ...
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bioRxiv - Biochemistry 2022Quote: ... An oligonucleotide probe against snoRNA58 labeled with [γ-32P] ATP by T4 polynucleotide kinase (Takara, cat# 2021A) was used as a loading control ...
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bioRxiv - Cell Biology 2020Quote: ... M-MLV reverse transcriptase and RNase inhibitor (TaKaRa, D2639A). Q-PCR was performed in triplicate with Power SYBR® Green (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2019Quote: ... and retrotranscription was performed with M-MLV retrotranscriptase (Clontech). Subsequently ...
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bioRxiv - Molecular Biology 2021Quote: ... and 1 µM of Shield-1 peptide (Clontech, Mountain View CA) for 4-6 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... and 1 μM of Shield-1 peptide (Clontech, Mountain View CA) for 4 h ...
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bioRxiv - Immunology 2024Quote: ... cDNA was subsequently synthesized using Reverse Transcriptase M-MLV (TakaRa) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: The VZV thymidine kinase gene (ORF36) was inserted into the Lenti-X pLVX-Puro vector (Clontech, Mountain View, CA) with XhoI and EcoR I sites ...
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bioRxiv - Bioengineering 2019Quote: ... MMEJ vector was modified so that 2A peptide and EGFP cDNA (Clontech) were inserted downstream of the SaCas9 driven by CMV promoter replaced for the GRK1-93 promotor ...
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bioRxiv - Microbiology 2023Quote: ... inverse PCR amplification was conducted with oligonucleotides and templates listed in Table S1 and the resulting PCR products were treated with T4 Polynucleotide Kinase (Takara) and ligated with a DNA ligation kit (Takara) ...
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bioRxiv - Molecular Biology 2023Quote: ... synthesized 5’ and 3’ fragments of the target RNA were 32P-radiolabeled at the 5’ end using T4 polynucleotide kinase (Takara). After gel purification ...
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bioRxiv - Molecular Biology 2022Quote: ... and digested with SacI and XbaI enzymes in M buffer (TaKaRa). The gene sequence included a SacI restriction site in the second intron and ...
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bioRxiv - Cancer Biology 2022Quote: ... Reverse transcription was performed using the M-MLV reverse system (Takara) to obtain cDNA ...
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bioRxiv - Microbiology 2021Quote: ... cDNA was synthesized using M-MLV reverse transcriptase (TaKaRa Biotechnology, Dalian, China). In brief ...
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bioRxiv - Immunology 2022Quote: ... and first strand cDNA was synthesized with Reverse Transcriptase M-MLV (TaKaRa). Real-time PCR was performed using Hieff® qPCR SYBR Green Master Mix (No Rox ...
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bioRxiv - Developmental Biology 2020Quote: ... Reverse transcription was performed by using reverse transcriptase M-MLV (TaKaRa, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... was subcloned into the lentiviral expression vector pLVX-M-puro (Takara 632164). For CRISPR-KO experiments ...
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bioRxiv - Neuroscience 2023Quote: ... followed by blocking solution containing primary antibodies against mCherry (mouse monoclonal 1:500, Takara Bio) and c-Fos (rabbit polyclonal 1:500 ...
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bioRxiv - Cell Biology 2023Quote: HaCaT cells were treated with the E-cadherin-blocking antibody (SHE78-7, Takara, Shiga, Japan), PY-60 (Axon Medchem ...
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bioRxiv - Biochemistry 2019Quote: ... CoAA and hnRNP M fragments were inserted in-frame into pM vector (Clontech) containing Gal4 DNA-binding domain to produce Gal4-fusion proteins ...
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bioRxiv - Microbiology 2021Quote: ... followed by cDNA synthesis using M-MLV reverse transcriptase (TaKaRa Biotechnology, Dalian, China). The qualified cDNAs were subjected to high-throughput sequencing for viral metagenomic analysis by the Beijing Genome Institute (BGI ...
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bioRxiv - Molecular Biology 2020Quote: ... The plug was equilibrated twice in 1 ml of 1× M buffer (TaKaRa) by rotating the tube for 30 min at room temperature ...
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bioRxiv - Plant Biology 2022Quote: ... cDNA synthesis was performed using the M-MLV reverse transcriptase (Takara Bio, Inc.) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... The plug was equilibrated twice in 1 mL of 1× M buffer (TaKaRa) by rotating the tube for 30 min at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... The plug was equilibrated twice in 1 mL of 1× M buffer (TaKaRa) by rotating the tube for 30 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were added to the blocking solution (Rabbit anti-DsRed 1:1000, stock #632496, Takara Bio ...
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bioRxiv - Developmental Biology 2021Quote: ... and reverse-transcribed using random hexamer-primers and M-MuLV reverse transcriptase (Takara, Japan). The resulted cDNA was used to perform quantitative PCR in an Applied Biosystems Step One Plus real-time PCR system (Applied Biosystems ...
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bioRxiv - Cell Biology 2020Quote: ... the reverse transcription reactions were performed using reverse Transcriptase M-MLV (RNase H-) (TaKaRa). The cDNA was then used for real-time qPCR using the SYBR Green qPCR Master Mix (Bi-make ...
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bioRxiv - Genomics 2019Quote: ... 9.0 μL of 3 SMART™ CDS Primer II A (12 M, Clontech, 634936), and 1.4 μL of Loading Reagent (20X ...
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bioRxiv - Microbiology 2024Quote: ... and cDNA was synthesized using the M-MLV Reverse Transcriptase cDNA Synthesis Kit (Takara) following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: ... After removal of the blocking solution 100 μL of anti-GFP (Living Colors GFP monoclonal antibody, Clontech) diluted 1:10,000 in antibody solution (1x PBS ...
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bioRxiv - Cell Biology 2019Quote: ... 2016) using anti-GFP (Living Colors A.v. peptide antibody, rabbit polyclonal, 1 mg/ml; TakaraBio/Clontech) primary antibody and horseradish peroxidase linked anti-rabbit IgG (from donkey ...
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bioRxiv - Cell Biology 2023Quote: Procollagen Type 1 C-peptide (PIP) was measured according to the manufacturer’s instructions (Takara, Shiga, Japan). For evaluation of PIP ...
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bioRxiv - Developmental Biology 2019Quote: ... The first strand cDNA was generated with M-MLV first strand cDNA synthesis kit (Takara), using oligo (dT ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 µg of total RNA was converted to cDNA using M-MLV reverse transcriptase (TAKARA) under standard conditions with oligo(dT ...