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Citations for Takara Bio :
1 - 50 of 5758 citations for Cow Upstream stimulatory factor 1 USF1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: Lenti-X™ p24 Rapid Titration ELISA Kit (TaKaRa) was used to determine virus titer ...
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bioRxiv - Molecular Biology 2022Quote: ... the GST gene from the pEGKG plasmid (29) was amplified by PCR and inserted immediately upstream of GFPS65T using the In-Fusion HD cloning kit (Takara).
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bioRxiv - Plant Biology 2019Quote: ... 2.5 kb of promoter sequences were isolated from genomic DNA and inserted upstream of the ß-glucoronidase gene of pCambia1301 vectors using the In-Fusion Cloning Recombinase kit (Clontech). The constructs were transformed into Agrobacterium tumefaciens strain GV3101 by electroporation ...
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bioRxiv - Cell Biology 2023Quote: ... templates were constructed by cloning the 1.5 kb upstream and downstream homology arms into the pPD95.77 plasmid using the In-Fusion Advantage PCR cloning kit (Clontech, Cat. #639621). Fluorescence tags were inserted into the constructs with a flexible linker before the stop codons ...
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bioRxiv - Cancer Biology 2020Quote: ... The titer of virus was measured using P24 ELISA kit (Clontech).The virus was aliquoted and stored at −80°C.
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bioRxiv - Plant Biology 2022Quote: ... we amplified the genomic fragment of the 4,194 bp upstream region of the translational initiation site from Tak-1 gDNA using PrimeSTAR Max DNA polymerase (Takara Bio), subcloned it into pENTR1A at the EcoRI restriction sites using the In-Fusion HD Cloning Kit and then transferred it into the destination vector pMpGWB10465 using Gateway LR Clonase II Enzyme mix (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2020Quote: ... viral titer was determined using the lentiviral p24 ELISA kit from Takara Bio (MountainView ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The isolation of the upstream genome sequences of gnrh1 and gnrh3 of piranha was performed using the Universal GenomeWalker ™ kit (Takara Bio USA) according to the instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Lentiviral titers were determined using the LentiX-p24 Rapid Titer ELISA kit (Takara). All cell culture reagents were obtained from ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Concentrated viruses were titered using the p24-Elisa kit (Takara Bio, cat. 632200) following manufacturer’s protocols ...
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bioRxiv - Microbiology 2023Quote: ... upstream and downstream flanking regions (approximately 1,000 bp) were amplified using PrimeStar HS (Takara Bio) and cloned into the vector pCE along with a 30 bp site specific spacer sequence (in the gene to be deleted ...
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bioRxiv - Bioengineering 2020Quote: ... and 21 were measured with an enzyme immunosorbent assay (ELISA) kit (Takara, Shiga, Japan).
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bioRxiv - Neuroscience 2020Quote: ... which were determined by an ELISA Adeno-X Rapid Titer Kit (Cat#: 631028, Takara). It detects the Adenoviral Hexon surface antigen ...
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bioRxiv - Immunology 2021Quote: ... Fgf9 cDNA was cloned upstream of the IRES-eGfp-SV40 polyA cassette (pIRES2-eGfp vector, Clontech) in the pTRE2 vector (Clontech)49 ...
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bioRxiv - Cancer Biology 2023Quote: ... The virus titer was determined with Lenti-X™ p24 Rapid Titration ELISA Kit (Takara), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... Coding sequence for RIIα was amplified from the library using primers Prkar2a_F & Prkar2a_R and inserted upstream of the IRES2 sequence in pIRES2-GFP (Clontech) using EcoRI and BamHI entry sites ...
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bioRxiv - Microbiology 2020Quote: ... Viral titer was determined by the ELISA p24 antigen assay (Lenti-X p24 Rapid Titer Kit, TaKaRa). Monocytes were infected immediately after purification by adding HIV-1BaL virus to the culture at MOI between 3 and 5 based on the p24 titer ...
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bioRxiv - Microbiology 2022Quote: ... Viral titer was determined by the ELISA p24 antigen assay (Lenti-X p24 Rapid Titer Kit, TaKaRa). Infections of fully differentiated MDM were performed after adherent growth in the presence of M-CSF for seven days ...
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bioRxiv - Plant Biology 2021Quote: ... The CaMV 35S promoter was then replaced by the HB40 native promoter (1,859 bp upstream translation start site) using In-Fusion (Takara) to generate the HB40:HB40-GFP construct ...
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bioRxiv - Molecular Biology 2024Quote: To generate APRF1pro:APRF1-mVENUS and TOPP4pro:TOPP4-3xFLAG the genomic region of both genes including a region of around 1.5 kb upstream their transcription start sites were amplified by PCR and cloned by the InFusion cloning (Takara) into the pCAMBIA1300 in frame with the coding sequences of mVENUS or the 3xFLAG peptide ...
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bioRxiv - Bioengineering 2021Quote: ... The virus titre was calculated using the ELISA-based Lenti-X™ p24 Rapid Titer Kit (Takara Bio) following the manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: The complementing strain was constructed by amplifying either nyxA or nyxB and their corresponding promoter region (500 bp upstream) with the PrimeStar DNA polymerase (Takara). Insert and pmini-Tn7 (47 ...
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bioRxiv - Microbiology 2019Quote: ... The complementing strain was constructed by amplifying BspL and its promoter region (500 bp upstream) with the PrimeStar DNA polymerase (Takara) using the following primers ...
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bioRxiv - Plant Biology 2020Quote: A reporter plasmid was made by cloning the 76bp AG intron sequence upstream of the lacZ gene in the vector pLacZi (Clontech). The yeast reporter strain was made by integration of the linearized AG intron reporter plasmid into the yeast strain YM4271 ...
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bioRxiv - Plant Biology 2022Quote: ... and pMKMM21 (3.5 kb upstream SYP12A:5’UTR:miniTurbo- Myc-SYP12A:3’UTR) were generated by in-fusion cloning (HD enzyme mix; Takara Bio). Gateway binary vectors pMKMM22 and pMKMM23 for the expression of miniTurbo-Myc- MpSYP13B and miniTurbo-Myc-MpSYP12A were generated by LR-recombination (LR clonase ...
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bioRxiv - Plant Biology 2021Quote: ... MEL1 and lacZ) under distinct GAL4 upstream activating sequences as described in Matchmaker GAL4 Two-Hybrid System 3 & Libraries User Manual (Clontech). The transformants were grown on synthetic dropout (SD ...
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bioRxiv - Cell Biology 2021Quote: ... pLPCX-IRES-eGFP was generated by cloning a fusion PCRP consisting of the encephalomyocarditis virus internal ribosomal entry site (EMCV-IRES) upstream of the eGFP coding sequence flanked by EcoRI and NotI sites into the cognate sites of pLPCX (Clontech). A DNA gene block encoding the vaccinia virus Wisconsin strain K3L fused to the C-terminus of mNeonGreen by a GSGS linker and hosted into the expression vector pTwist Lenti SFFV puro WPRE was obtained commercially (Twist Bioscience) ...
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bioRxiv - Cell Biology 2023Quote: ... The upstream and downstream regions adjacent to the binding site were amplified using Advantage 2 polymerase (Takara Bio, Shiga, Japan) from 3T3-L1 genomic DNA and cloned into the HR110-PA-1 vector (System Biosciences) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 2019) upstream of an egfp coding sequence and an SV40 polyadenylation sequence using In-Fusion Snap Assembly master mixes (Takara). To prepare the injection mixtures ...
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bioRxiv - Microbiology 2024Quote: ... and the Tn7 site homologous fragments (upstream and downstream) were amplified from AB5075 from genomic DNA using the PrimeStar polymerase (Takara).
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bioRxiv - Physiology 2022Quote: ... The insulin-like growth factor 1 (IGF1) coding sequence was PCR amplified (CloneAmp, Takara Bio; Cat. No. 639298) from cDNA generated from a rat gastrocnemius muscle ...
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bioRxiv - Biophysics 2024Quote: Lentivirus titration was determined by p24 ELISA using Lenti-X™ p24 Rapid Titer Kit (Takara Bio, no. 632200) and a plate reader (PerkinElmer Victor3 V) ...
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bioRxiv - Immunology 2021Quote: ... and F1 and F2 arms homologous to upstream and downstream sequences of the A41L ORF using in-fusion cloning (Takara Bio). The resultant spike transfer cassette was linearized by NotI and transfected into the MCL for homologous recombination with SCV ...
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Lateral organ diversification in plants mediated by the ALOG protein family of transcription factorsbioRxiv - Plant Biology 2019Quote: ... an MpTAW1 genomic fragment with a 10 kb upstream region and a 3 kb downstream region was amplified by PCR using Prime STAR GXL polymerase (TaKaRa, Japan) and subcloned into pENTR/D-TOPO (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... These fragments were cloned into pUC19 with the HYG resistance cassette upstream of pCTR4-2 with InFusion cloning (Takara, Cat#638911). Plasmids were isolated from individual clones and confirmed by digestion with HindIII (New England Biolabs ...
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bioRxiv - Microbiology 2023Quote: ... the V5-tag sequence followed by a Stop codon was introduced upstream of the IRES in the pLVX-IRES-Neo vector (Clontech, Addgene), using annealed oligo-cloning ...
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bioRxiv - Biochemistry 2023Quote: ... The fusion between the core of the AGA1 promoter (-150 to 0) and the STL1 upstream activation sequence (-800 to -163) was generated by In-Fusion cloning (Takara Bio) into a plasmid containing the 24xPP7 stem loops ...
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bioRxiv - Developmental Biology 2022Quote: ... A synthetic CRE sequence flanked by AttB1-AttB2 sites was cloned (by Eurofins) upstream of a IRES2-DsRed-express2 cassette (plasmid pIRES2-DsRed-express2 from Clontech/SakaRa, #632540), producing a plasmid called p4 containing AttB1-CRE-AttB2_IRES2-DsRed-express2 ...
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bioRxiv - Plant Biology 2023Quote: ... the genomic sequence from the MpRKD promoter region (3827 bp upstream from start codon) to the 3’UTR was amplified by PCR with PrimeSTAR HS DNA polymerase (TaKaRa Bio, Kusatsu, Japan) and the primers IF-Hind-pRKD-F and IF-Sal-gRKD-R ...
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bioRxiv - Developmental Biology 2024Quote: ... PCR-amplified enhancer-containing fragments were tandemly inserted into the upstream region of the original promoter sequence by In-Fusion cloning (TAKARA BIO, Shiga, Japan). This process is summarized in Figure S3 ...
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bioRxiv - Molecular Biology 2021Quote: ... we performed ELISA against HIV p24 (TaKaRa Clontech #632200) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... we performed ELISA against HIV p24 (TaKaRa Clontech #632200) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... and titer quantified using p24 ELISA antigen assay (Takara). MOI=5 was used to transduce 1x1097 EndoC-βH3 cells in culture media without pen/strep and puromycin.
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bioRxiv - Cell Biology 2023Quote: ... downstream of the Myh6 (α- myosin heavy chain) promoter and upstream of a human growth hormone polyadenylation signal using In-Fusion HD (Takara Bio cat# 011614). The final transgenic targeting construct was verified by DNA sequencing ...
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bioRxiv - Microbiology 2021Quote: ... An anti-histidine antibody was used for ELISAs as positive control (Takara, catalog # 631212).
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bioRxiv - Cancer Biology 2021Quote: ... a WST-1 cell proliferation assay kit (MK400, Takara Bio) was used according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... and as a prey to the activation domain of the GAL4 transcription factor (GAL4-AD) in plasmid pACT2 (Clontech). To monitor the induction of a HIS3 reporter gene by complexes of bait and prey fusion proteins ...
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bioRxiv - Biochemistry 2024Quote: ... The sequence of human histone H2A(K15C-129) was cloned into the pCold-Trigger factor (TF) vector (Takara Bio) with a SUMO coding sequence inserted to generate His6–TF–SUMO–H2A(K15C-129 ...
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bioRxiv - Immunology 2020Quote: Levels of p24 antigen in the purified SARS-CoV-2 pseudotype solution was measured by ELISA (Takara). Mouse sera were heat inactivated ...
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bioRxiv - Cell Biology 2023Quote: candidate factors were prepared by subcloning the ORF template clones by PCR amplification with Prime STAR GXL DNA polymerase (Takara) and ligation by In-Fusion cloning enzyme (Clontech).