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Citations for Takara Bio :
301 - 350 of 6153 citations for Cow T Cell Surface Glycoprotein CD3 Gamma Chain CD3G ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Adeno-associated virus (AAV) cell-lysates were produced using the AAVpro Purification Kit (All Serotypes) (Takara) with slight modifications ...
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bioRxiv - Immunology 2024Quote: ... banked cell pellets were rapidly thawed and processed using the Nucleospin® Blood XL kit (Takara) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... The upstream and downstream homologous arms of each TF obtained by PCR amplification were introduced along with the pyrG marker into a pMD20 T-Vector (TaKaRa, Japan) by NEBuilder (New England Biolabs ...
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bioRxiv - Plant Biology 2020Quote: ... For Yeast-Two-Hybrid assays HvRIPb and HvRIPb truncations were subcloned from the pGEM-T easy vector into pGADT7 and pGBKT7 plasmids (Clontech Laboratories) using the EcoRI and BamHI restriction sites ...
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bioRxiv - Microbiology 2021Quote: ... and the amplified fragments were cloned into pMD19-T vector with Mighty ta-cloning Reagent Set for Prime STAR (Takara, Japan), and verified by sequencing ...
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bioRxiv - Epidemiology 2019Quote: ... The PCR products of the three fragments were cloned into a pMD18-T easy vector (TaKaRa Biotechnology Co., Ltd., Dalian, China) for later sequencing (Syn-Biotechnology ...
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bioRxiv - Plant Biology 2020Quote: ... plant intron Syn7 [34] regulated by a double-enhancer Cauliflower mosaic virus 35S RNA promoter (P35S2) and a nos terminator in the T-DNA binary vector pBI121 (Clontech, Switzerland). Syn7 was amplified with the primers 5′- GAGCTGAAAGggtaagattatcgatatttaaattatttatttcttcttttc-3′ and 5′-TGAAGTCGATGCctgcatgatatcataaaaccatgga-3′ specifying the splicing junctions and then inserted into the YFP coding region by overlapping PCR ...
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bioRxiv - Biochemistry 2020Quote: ... The targeted gene and its flanking sequences were amplified by PCR with Synechocystis chromosome DNA as the template and cloned into pMD18–T vector (Catalog No: D101A, Takara, Japan). The physical map of the Synechocystis genome region containing sll0445–sll0447 or sll1334 ...
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bioRxiv - Molecular Biology 2021Quote: Gene-specific target sequences as well as a heterologous fragment from Mus musculus (Muslta) used for double-stranded RNA (dsRNA) synthesis were amplified and cloned into pMD™ 19-T Vector (Takara). Templates used for single-stranded RNA were amplified with primers that incorporated with the T7 promoter sequence (S4 Table) ...
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bioRxiv - Plant Biology 2022Quote: ... PCR products were amplified with specific primers based on the off-target sites and purified products were cloned into pMD19-T vector (TaKaRa, Japan) for Sanger sequencing ...
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bioRxiv - Neuroscience 2023Quote: Artificially synthesized (G4C2)50 sequences flanked at the 5′ end with an EagI recognition site and at the 3′ end with a PspOMI recognition site were subcloned into T-vector pMD20 (Takara Bio). To generate a longer repeat size ...
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bioRxiv - Cell Biology 2023Quote: ... The copy number was calculated using the standard curves for cloned Tert and Dloop in a pMD19-T Easy vector (TaKaRa, 3271), respectively ...
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bioRxiv - Cell Biology 2020Quote: ... RNA was isolated from cells using the NucleoSpin RNA Plus kit of Clontech Laboratories (Takara Bio Company). cDNA was constructed using the In-Fusion SMARTer Directional cDNA Library Construction kit of Takara Bio ...
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bioRxiv - Cancer Biology 2021Quote: Total RNA was extracted from cells and quantified using RNA Isolation Reagent kit (9109, Takara, Tokyo, Japan). cDNA was obtained from 1 µg of total RNA from each sample (Super M-MLV reverse transcriptase ...
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bioRxiv - Biochemistry 2020Quote: ... and cell toxicity was evaluated using the LDH Cytotoxicity Detection Kit (Cat #MK401; Takara Bio, Shiga, Japan).
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bioRxiv - Neuroscience 2020Quote: Cell line CUT&RUN libraries were prepared using the SMARTer ThruPLEX DNA-seq Kit (Takara Bio R400676), with the following PCR conditions ...
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bioRxiv - Microbiology 2020Quote: ... Protein concentrations of cell lysates were measured using a bicinchoninic acid protein assay kit (Takara Bio Inc.). Cell lysate containing the same amount of protein was mixed with 10 volumes of methanol and then centrifuged at 20,000 × g for 30 min to precipitate proteins ...
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bioRxiv - Microbiology 2020Quote: Genomic DNA was isolated from Vero cells using the NucleoSpin Tissue kit (Takara Bio Inc., Shiga, Japan). To determine the sequence of the N-terminal PVR1- and PVR2-coding regions ...
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bioRxiv - Cell Biology 2022Quote: ... Cell pellets was collected and isolated for total RNA with MiniBEST Universal RNA Extraction Kit (Takara, #9767). Total RNA (1 μg ...
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bioRxiv - Molecular Biology 2022Quote: Single cells were prepared using the STORM-seq protocol (referencing the SMART-Seq Stranded Kit – Takara Bio) on the SPT Labtech Mosquito (HV) ...
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bioRxiv - Neuroscience 2019Quote: ... Lysing of cells and particle purification were carried out using AAVpro® Purification Kit (All Serotypes, TAKARA) according to the manufacturer’s instructions ...
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Maternal transmission of a paramutant phenotype requires intact DNMT2 functions in the male germlinebioRxiv - Genetics 2020Quote: ... The single cell cDNA libraries were made using SMART-Seq v4 Ultra Low Input RNA Kit (Takara). The cDNAs were fragmentized and Illumina Sequencing adaptors were added using Nextera DNA Library Preparation Kit ...
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bioRxiv - Developmental Biology 2021Quote: ... AAVs particles were then purify from AAV-producing cells using AAVpro Purification Kit Maxi (Takara, Cat. #6666).
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bioRxiv - Developmental Biology 2021Quote: ... RNA were extracted from each well and converted into cDNA using SAMRT-Seq Single cell kit (Takara). DNA library was made and sequenced at Single Cell Genomic Core at the Baylor College of Medicine ...
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bioRxiv - Physiology 2021Quote: ... Adenovirus was generated in HEK293A cells and titered using the Adeno-X Rapid Titer Kit (Takara Bio). For differentiation ...
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bioRxiv - Cancer Biology 2022Quote: ... gDNA from all cells were isolated using the Machery Nagel L Midi NucleoSpin Blood Kit (Clontech, 740954.20). Modifications to the manufacturer’s instructions were added as follows ...
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bioRxiv - Cancer Biology 2023Quote: ... bacteria cells were pelleted and BAC DNA was extracted using the NucleoBond Xtra BAC kit (Takara, 740436) as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: All viruses and lentiviruses were produced by transfecting Hek293T cells using CalPhos Mammalian Transfection Kit (Takara Bio) according to the manual in DMEM ...
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bioRxiv - Genomics 2024Quote: ... Both reagents were included in SMART-Seq Single Cell PLUS kit (Takara Bio, San Jose, CA, U.S.). Immediately after sampling in lysis buffer ...
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bioRxiv - Molecular Biology 2019Quote: ... The amplified fragment was cloned into the EcoR I and BamH I restriction enzyme sites of pMD18-T vector (TaKaRa, Kusatsu, Japan) and verified by sequencing ...
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bioRxiv - Microbiology 2022Quote: ... were synthesized in vitro transcription based on the corresponding cDNAs inserted into the pGEM-T easy vector with T7 RNA polymerase (Takara, Beijing, China) as previously described (22) ...
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bioRxiv - Plant Biology 2023Quote: ... and ARR1 (2070 bp) was generated using cDNA of Col-0 as template and ligated into T-vector pMD20 (Takara Bio, Japan). All the primers used for cloning were listed in Table S1 ...
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bioRxiv - Developmental Biology 2020Quote: Total RNA was extracted from cells using the NucleoSpin RNA II kit (Clontech Laboratories, Mountain View, CA, USA). The quality and quantity of total RNA were measured by Nanodrop 1000 (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2021Quote: RNA from FACS-sorted cells ranging from 3,000 – 10,000 were isolated with the NucleoSpin RNA XS kit (Clontech) with on column DNase digestion according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... cells and medium were collected and AAV particles were purified by AAVpro Purification Kit (Takara Bio; Shiga, Japan) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: cDNA was generated from single cells in the 96-well plate using the SmartSeq v4 kits (Takara Bio) using 1/4th volume reactions dispensed using a Mantis dispenser (Formulatrix) ...
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bioRxiv - Biochemistry 2019Quote: ... Lentivirus was produced by transient transfection of HEK 293T cells using the CalPhos mammalian transfection kit (Clontech laboratories) to introduce packaging vectors (VSVg and GAG/POL/Δ8.2 ...
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bioRxiv - Cell Biology 2021Quote: Total RNA was isolated from wild-type or transiently transfected cells with MiniBEST Universal RNA Extraction Kit (Takara), an additional DNase I (NEB ...
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bioRxiv - Cell Biology 2021Quote: Total genomic DNA of HeLa cells was extracted after irradiation using Total genomic DNA extract kit (TAKARA, China). Human nuclear GAPDH gene (forward ...
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bioRxiv - Immunology 2020Quote: cDNA from sorted cells was generated using the SMART-Seq v4 Ultra low input RNA kit (Clontech # 634890) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were sorted directly into buffer for SMART-Seq v4 Ultra Low Input RNA Kit (Clontech Laboratories, Inc.). Subsequent processing was carried out by the UCL Genomics sequencing facility ...
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bioRxiv - Developmental Biology 2023Quote: ... 18-20 μg of indicated plasmids were transfected in HEK293T cells using the CalPhos Mammalian Transfection Kit (Takara). After 48h ...
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bioRxiv - Biochemistry 2023Quote: ... Genomic DNA from WT and Strt1−/− cells was extracted with a NucleoSpin Tissue kit (Takara Bio, Shiga, Japan) and subjected to PCR using specific primers (Oligonucleotide sets 3 ...
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bioRxiv - Cell Biology 2023Quote: cDNA was synthesized from the total RNA of HapT1 cells using the PrimeScript RT Reagent Kit (Takara Bio). The amounts of Pts and Actb were quantified on a LightCycler 480 (Roche ...
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bioRxiv - Developmental Biology 2024Quote: Single-cell cDNA synthesis was performed using SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara) according to the manufacturer’s protocol with some modifications as previously described17 ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was extracted from drug/vehicle-treated cell lines using NucleoSpin RNA kit (Takara, Catalog no. 740955.50). 2 ug of total RNA was used to synthesize cDNA with qScript Ultra Supermix (Quantabio ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were blocked in 5% Skim milk powder (Fujifilm, Cat# 190-12865) with Phosphate buffered saline with tween® (PBS-T) (Takara, Cat# T9183). The primary antibody was incubated overnight at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... membranes were then blocked in 5% Skim milk powder (Fujifilm, Cat# 190-12865) mixed with Phosphate buffered saline with tween® (PBS-T) (Takara, Cat# T9183). The chosen primary antibody was then incubated overnight at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: 20 cm plates of HEK293T cells were transfected with 10 µg of each plasmid using CalPhos transfection kit (Takara) according to manufacturer’s instructions and harvested 3 days later ...
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bioRxiv - Immunology 2021Quote: Cell death was assessed by the release of LDH in conditioned medium using LDH Cytotoxicity Detection Kit (TaKaRa, CA).