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Citations for Takara Bio :
351 - 400 of 6128 citations for Cow G Protein Coupled Bile Acid Receptor 1 GPBAR1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2022Quote: ... cDNA was synthesized from 1 μg of total RNA using the PrimeScript RT reagent kit (Perfect Real Time) (Cat No RR037A, TaKaRa) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... First-strand cDNA was synthesized from 1 μg of total RNA using the PrimeScript™ RT Master Mix Kit (Takara). SYBR Premix Ex Taq™ kit (Takara ...
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bioRxiv - Plant Biology 2023Quote: ... cDNAs were prepared from 1 ng of total RNA using the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (TaKaRa) according to the manufacturer guidelines for cDNA synthesis and amplification ...
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bioRxiv - Microbiology 2022Quote: ... 1 ng of total RNA was pre-amplified with the SMARTer Ultra Low Input kit v4 (Clontech, Mountain View, CA) per manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2023Quote: ... Reverse transcription (RT) reactions were performed with 1 μg total RNA using the PrimeScript RT kit with gDNA Eraser (Takara). The resulting cDNA was amplified in triplicate on the 480 Real-Time PCR system (Roche ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Reverse transcription (RT) reactions were performed with 1 μg total RNA using the PrimeScript RT kit with gDNA Eraser (Takara). The resulting cDNA was amplified in triplicate on the 480 Real-Time PCR system (Roche ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 μg of total RNA was reverse transcribed into complementary DNA (cDNA) using PrimeScript™ RT Reagent Kit (Takara, RR047B). Amplification of cDNA product was performed using specific primers with the TB Green® Premix Ex Taq™ II (Takara ...
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bioRxiv - Microbiology 2024Quote: ... The PCR product was run on a 1% agarose gel at 85 V for 35 min and gel extracted using the Nucleospin Gel Extraction Kit (Takara). We then used PCR with KOD Polymerase 2x Mastermix (Millipore Sigma ...
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bioRxiv - Immunology 2024Quote: ... we ran our linearized plasmid backbone and 83 bp PCR products on a 1% agarose gel and recovered each using the Gel and PCR Clean-up Kit (Takara) per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... two to three amino acids in GFP-SP-BAP were replaced by alanine for each construct using the CloneAmp HiFi PCR Premix (Takara Bio) according to the manufacturer.
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bioRxiv - Neuroscience 2022Quote: ... Pak1 fragment (amino acids 270–521) was PCR-amplified from pCMV6M-Pak1 and subcloned into pCold-Pros2 vector (Takara Bio Inc.) to generate pCold-Pros2-Pak1cat ...
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bioRxiv - Developmental Biology 2021Quote: ... the RNA-protein mix was catalyzed by proteinase K (Takara, 9034) at 55 °C for 1 h ...
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bioRxiv - Genomics 2019Quote: ... and proteins extracted with affinity purification using Cobalt-Talon beads (Clontech). Site-directed mutagenesis for Exd and Hth was performed via amplification of the original plasmid with primers harboring single amino acid replacements (arginine to alanine ...
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bioRxiv - Cell Biology 2022Quote: ... E.coli competent cells transformed with pTf16 chaperone protein tag (TaKaRa Bio) per the manufacturer’s protocol.
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bioRxiv - Neuroscience 2021Quote: ... The enhanced green fluorescent protein (EGFP) vector was obtained from Clontech Europe ...
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bioRxiv - Microbiology 2022Quote: ... S-6P-NanoLuc proteins were released after HRV 3C protease (TaKaRa) treatment overnight at 4°C.
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bioRxiv - Biochemistry 2022Quote: ... The proteins were purified using TALON Metal Affinity Resin (Takara Bio) and eluted with a buffer containing 200 mM imidazole ...
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bioRxiv - Biochemistry 2023Quote: ... The protein was purified by metal-affinity chromatography (Talon resin, Clontech), the affinity tag removed using tobacco etch virus (TEV ...
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bioRxiv - Cell Biology 2023Quote: ... His6-tagged proteins were purified with Talon metal affinity resin (Clontech) using the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the protein concentration was assessed and quantified using BCA (TaKaRa, Japanese). Immunoblotting was conducted on cell and tissue extracts (30 μg total protein/well ...
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Caspase cleavage of Influenza A virus M2 disrupts M2-LC3 interaction and regulates virion productionbioRxiv - Microbiology 2024Quote: ... The expressed protein was purified by TALON Metal Affinity Resin (Clontech), cleaved by TEV protease ...
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bioRxiv - Molecular Biology 2023Quote: ... GST-tagged protein was eluted with 6ml glutathione (10mg/ml, Takara) and the flow-through was collected ...
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bioRxiv - Synthetic Biology 2020Quote: HEK293T cells were transiently transfected with pLenti-EF-blast vectors together with pCAG-HIVgp and pCMV-VSV-G-RSV-Rev (provided by RIKEN BRC, Ibaraki, Japan) using TransIT-2020 Reagent (TaKaRa). The medium ...
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bioRxiv - Biochemistry 2020Quote: ... After high speed centrifugation (40 000 x g/40 min/4 °C) the supernatant was loaded on to a gravity TALON metal affinity column (Takara), equilibrated and washed with 10 CV buffer A/20 mM imidazole ...
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bioRxiv - Biochemistry 2020Quote: ... After high speed centrifugation (40 000 x g/40 min/4 °C) the supernatant was loaded on a HiTrap (GE) TALON Crude (Takara) metal affinity column ...
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Potential for virus endogenization in humans through testicular germ cell infection: the case of HIVbioRxiv - Microbiology 2020Quote: ... or pBR_NL4-3 92TH014.12 IRES eGFP (R5 tropic) [81] (a kind gift from Frank Kirchhoff, Ulm University, Germany) and VSV-G envelope encoding plasmid (PT3343-5, Clontech). 293T cell supernatants were collected 3 days post-transfection ...
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Potential for virus endogenization in humans through testicular germ cell infection: the case of HIVbioRxiv - Microbiology 2020Quote: ... VSV G-pseudotyped HIV-1 using full-length HIV-1 molecular clone pNL4-3 [79] or the R5-tropic pNL4-3 AD8 derivative [80] and VSV-G envelope encoding plasmid (PT3343-5, Clontech); 4 ...
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bioRxiv - Cell Biology 2021Quote: pAAVS1-CMV-HA-TRF2 and variants encoding alanine-substitution mutants of TRF2 (used in Figure 1E-G) were prepared using the In-Fusion (Clontech) reaction ...
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bioRxiv - Cancer Biology 2022Quote: ... in which pRetroX-TetOne puro empty vector or pRetroX-TetOne puro-ALKBH5 were each cotransfected with the envelope vector VSV-G using Xfect transfection reagent (Clontech). BEAS2B ...
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bioRxiv - Cell Biology 2022Quote: ... VSV-G pseudotyped retroviral particles encoding constructs of choice were prepared using standard protocols using GP2-293 packaging cells (Clontech). Viral supernatants were collected and used to infect target cells by centrifugal inoculation (spinoculation) ...
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bioRxiv - Cell Biology 2020Quote: ... was co-transfected with packaging plasmid (pCAG-HIVgp) and VSV-G/Rev-expressing plasmid (pCMV-VSVG-RSV-Rev) into HEK293T cells (Clontech). Cells expressing Involucrin-mCherry were selected by 2 µg/ml Puromycin treatment.
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bioRxiv - Immunology 2021Quote: ... The retroviral transfer vector was co-transfected with pVSV-G retroviral envelope-expressing plasmid into the GP2-293 cell line (Clontech-TaKaRa) using Lipofectamine 2000 reagent (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were clarified by centrifugation at 3,000 x g and incubated with Talon® immobilized metal affinity resin (Takara #635502) for 2 hours at 4ºC ...
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bioRxiv - Microbiology 2022Quote: ... The resultant lysate was cleared by centrifugation at 30 000 x g for 20 min and applied to Talon CellThru Co2+ chelating resin (TaKaRa-Clontech). The protein bound resin was washed with 3 column volumes (CV ...
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bioRxiv - Cancer Biology 2023Quote: ... Additional lentiviral vectors were packaged in HEK 293T cells using Lenti-X Packaging Single Shots (VSV-G) (Takara Bio USA) per the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... the insoluble material was removed by 50k x g centrifugation for 30 min and the supernatant was added to Talon resin (Clontech) with 20 mM imidazole to bind overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was obtained by centrifugation of the cell lysate at 10,000 g for 30 minutes and applied into the His TALON™ gravity column (Clontech). The columns were washed to remove the non-target proteins ...
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bioRxiv - Cancer Biology 2023Quote: ... The generated lentiviral plasmids were cotransfected with viral packaging plasmids PAX2 and pMD2.G into HEK 293T Lenti-X cells (Clontech) using TransIT-LT1 (Mirus Bio LLC ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was collected after centrifugation at 65,000 g for 35 min and was incubated with TALON® Metal Affinity Resin (TaKaRa) for 2 h at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... with psPAX2 and pMD2.G viral particles harvested during the 16-72 h window post-transfection were concentrated with Lenti-X (Clontech) and stored at -80°C ...
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bioRxiv - Immunology 2022Quote: ... Seq Kit (Takara), as per the manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 kit (Takara) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... mRNA was retro-transcribed by Takara kit (Takara Bio), GAPDH was used as internal reference ...
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bioRxiv - Biophysics 2019Quote: ... D40A mutation and deletion of C-terminal 10 amino acids were introduced into pET15-sfGFP-minD by using the PrimeSTAR Max mutagenesis protocol (TaKaRa, Shiga, Japan). Similarly ...
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bioRxiv - Genetics 2021Quote: ... The cDNA samples were prepared from 1 μL total RNA per reaction using a reverse transcription kit (Takara Biotechnology, Tokyo, Japan) and stored at - 20°C for qRT-PCR assays ...
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bioRxiv - Developmental Biology 2021Quote: ... and 1 ng of RNA was used for cDNA synthesis (SMART-Seq v4 Ultra Low Input RNA kit – Takara Bio: #634898). Verification of library quality and sequencing were done as for the embryonic dataset (see above) ...
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bioRxiv - Synthetic Biology 2021Quote: ... An aliquot of 1 μg of DNAse treated RNA was used for cDNA synthesis using the PrimeScript™ RT-PCR Kit (Takara) in a final volume of 20 μL according to the manufacturer indications ...
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bioRxiv - Cell Biology 2021Quote: ... with primers BA1569/BA1570 and cloned into RSFDuet-1 using BamHI/EcoRI sites with an In-Fusion HD Cloning Plus kit (Takara Bio). To make pBA2276 (His6-Designed zinc finger) ...
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bioRxiv - Developmental Biology 2022Quote: ... An aliquot of 1 μg of the total RNA was used to synthesize cDNA using PrimeScript™ RT reagent Kit with gDNA eraser (Takara). qRT-PCR analysis was performed on a StepOnePlus Real-Time PCR system (Applied Biosystems ...
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bioRxiv - Plant Biology 2022Quote: ... The 1 μg total RNAs were used to synthesize cDNA with the PrimeScript™RT reagent Kit with gDNA Eraser (Takara). qRT-PCR was carried out with SYBR Premix Ex Taq II Premix (Takara ...