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Citations for Takara Bio :
401 - 450 of 5945 citations for Cortisol ELISA Kit 1 Whole plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... PrimeScript™ RT Reagent Kit (TAKARA) was used to generate cDNA and FastStart Essential DNA Green Master (Roche ...
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bioRxiv - Cell Biology 2023Quote: ... using In-Fusion Cloning kit (Takara).
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bioRxiv - Genomics 2023Quote: ... SMART-Seq v4 kit (Clontech, 634893) was used for the reverse transcription and cDNA amplification according to the manufacturer’s specifications ...
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bioRxiv - Immunology 2023Quote: Using PrimeScript RT reagent Kit (TaKaRa), 0.5 µg of total RNAs were reverse transcribed into complementary DNA ...
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bioRxiv - Cell Biology 2022Quote: ... with the SYBR premeraseTaq kit (TAKARA). Amplified mRNA levels were normalized to 18s mRNA ...
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bioRxiv - Molecular Biology 2023Quote: ... retrotranscribed using PrimeScript Reagent Kit (Takara) and amplified by RT-qPCR using PowerUp SYBR-Green MasterMix (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... SMARTer PCR cDNA synthesis kit (Clontech) was used according to the manufacturer’s specifications ...
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bioRxiv - Neuroscience 2023Quote: ... and AAV titration kit (TaKaRa/Clontech). The purity of viruses was routinely checked by silver staining (Pierce ...
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bioRxiv - Neuroscience 2023Quote: ... and AAV titration kit (TaKaRa/Clontech). The purity of viruses was routinely checked by silver staining (Pierce ...
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bioRxiv - Molecular Biology 2023Quote: ... by In-fusion cloning kit (Takara).
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bioRxiv - Molecular Biology 2023Quote: ... by In-fusion cloning kit (Takara).
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bioRxiv - Cell Biology 2023Quote: ... using In-Fusion cloning kit (Takara). We verified the sequences of both constructs pHR-[SFFV/FLAG::TurboID] and pHR-[SFFV/FLAG::TurboID::SQSTM1] using Sanger sequencing ...
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bioRxiv - Neuroscience 2023Quote: ... using Infusion kit (Takara catalog # 638949). For some enhancers we instead cloned into a next generation reporter vector backbone that includes a SYFP2-P2A-3xFLAG-H2B reporter for detection of cytosolic SYFP2 and nuclear FLAG for simultaneous expression analysis and snRNA-seq ...
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bioRxiv - Molecular Biology 2023Quote: The Universal GenomeWalker 2.0 kit (Clontech Laboratories Inc. ...
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bioRxiv - Molecular Biology 2023Quote: ... In-Fusion HD Cloning Kit (Clontech) was used for this reaction.
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bioRxiv - Genetics 2023Quote: ... PrimeScript RT Reagent Kit (Takara, RR037) was used for cDNA synthesis ...
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bioRxiv - Cell Biology 2023Quote: ... E-cadherin antibody (HECD-1, 1:1000 IF, 1:1000 WB) was from Takara Bio ...
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bioRxiv - Neuroscience 2019Quote: ... Rabbit anti-dsRed (Clontech, 1:500-1:1000), Sheep anti-GFP (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... rabbit anti-DsRed (1:200-1:500, Clontech), mouse anti-1D4 anti-Fasciclin II (1:10 ...
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bioRxiv - Plant Biology 2020Quote: ... The cDNA library was ligated to pGADT7-Rec vector to prepare recombinant AD construct using Make Your Own “Mate and Plate” Library system (Clontech). The yeast strain Y2H gold was co-transformed with bait and prey recombinant construct and colonies were screened against DDO (SD/-Leu/-Trp ...
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bioRxiv - Genomics 2021Quote: The NucBlue and DAPI co-stained single-nuclei suspension (60□cells/µl) was distributed to eight wells of a 384-well source plate (Takara) and then dispensed into a barcoded SMARTer ICELL8 3’ DE Chip (Takara ...
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bioRxiv - Genomics 2019Quote: ... into individual wells of a 48-well plate (Brand) filled with 4 μl of a hypotonic lysis buffer containing 2 U/μL RNase inhibitor (Takara) and 0.2 % Triton-X-100 in Nuclease-free water ...
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bioRxiv - Immunology 2020Quote: ... Transient retroviral supernatants were produced as previously described.44 Activated NK cells were purified and transduced with retroviral supernatants on day +4 in human fibronectin-coated plates (Clontech Laboratories ...
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bioRxiv - Neuroscience 2020Quote: ... Single cells were sorted into 96-well plates containing 4uL lysis buffer containing 4U Recombinant RNase Inhibitor (Takara Bio 2313B), 0.05% Triton X-100 ...
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bioRxiv - Biophysics 2021Quote: ... Cells were seeded at 25-30% confluency onto the prepared coverslips placed in 6-well plates in cell imaging media comprising phenol-red free DMEM (HyClone) supplemented with 10% (v/v) FBS (ClonTech) and 1 mM sodium pyruvate and allowed to settle for at least 4h ...
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bioRxiv - Microbiology 2022Quote: ... cells were transduced in 24-well plates precoated with recombinant fibronectin (FN CH-296, Retronectin Takara, Clontech, Mountain View, CA) with retroviral supernatants and expanded in complete medium (45% RPMI-1640 and 45% Click’s medium ...
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bioRxiv - Microbiology 2022Quote: ... cells were transduced in 24-well plates precoated with recombinant fibronectin (FN CH-296, Retronectin Takara, Clontech, Mountain View, CA) with retroviral supernatants and expanded in complete medium (45% RPMI-1640 and 45% Click’s medium ...
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bioRxiv - Immunology 2020Quote: ... for 24 h and transduced with lentiviral particles by spinfection (1000 x g for 90 min at 32°C) in the presence of Polybrene (5 μg/ml) on the plates coated with Retronectin (50 μg/ml) (Takara/Clontech) and anti-CD3 (1–2 μg/ml) ...
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bioRxiv - Cell Biology 2020Quote: ... Mouse E-Syt3 cDNA was amplified by PCR from a cDNA library constructed with poly(A) RNA from 3T3-L1 adipocytes (Mate & Plate TM Library System, Clontech) and cloned into the PT-Adv vector using 5’and 3’ E-Syt3 primers (Table 1) ...
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bioRxiv - Cell Biology 2021Quote: ... 5 × 104 SH-SY5Y cells in 90 µL of growth media containing no antibiotics were plated in opaque TC-treated 96-well plates containing Xfect (Clontech)/DNA transfection complexes ...
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bioRxiv - Molecular Biology 2021Quote: ... First strand cDNA was synthesized with 3.6 μg of total RNA using Make your own ‘mate & plate’ library system (Takara, Clontech) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2022Quote: ... A tomato Mate & Plate library was constructed and used for screening with the Matchmaker Gold Yeast Two-Hybrid System (Clontech) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: iPSCs SFCi55 and hESCs RUNX1-GFP were plated at 3 × 105 cells per a well of a 6 well plate and reverse transfected with 2 μg of DNA using the Xfect Transfection reagent (Clontech) and analyzed 2 days later.
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bioRxiv - Immunology 2022Quote: ... Lentiviral supernatants were spun onto nontreated wells plates coated with 30 μg/mL Retronectin reused up to three times (T100B, Takara), at 2000 x g for 2 hours at 32°C and replaced with activated human CD8+ T cells in fresh RPMI supplemented with 30 U/mL IL-2.
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bioRxiv - Plant Biology 2022Quote: ... which was conducted with the “Make Your Own “Mate & Plate™” Library System” according to the manufacturer’s instructions with minor modifications (Clontech). Oligo-dT primers provided in the kit were used for first-strand cDNA synthesis with 2 µg total RNA as input ...
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FTSH3 facilitates complex I degradation through a direct interaction with the complex I subunit PSSTbioRxiv - Plant Biology 2023Quote: ... Mating was carried out in a 96-well plate according to manufacturer’s instructions (Matchmaker GAL4 Two Hybrid System (Clontech/TakaraBio). Mated diploid strains were serially diluted and plated on - Leu–Trp double dropout (DDO ...
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bioRxiv - Plant Biology 2023Quote: ... The cDNA library was prepared from rice flowers and ligated to the pGADT7-Rec vector using the Make Your Own “Mate and Plate” Library System (Takara). A second cDNA library was prepared from rice leaves under salt stress ...
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bioRxiv - Immunology 2023Quote: ... and GC B cells (live singlet CD19+ CD4− IgDlo CD71+CD38int CD20+ CXCR5+) were sorted using a FACSAria II into 96-well plates containing 2 μL Lysis Buffer (Clontech) supplemented with 1 U μl−1 RNase inhibitor (NEB) ...
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bioRxiv - Cancer Biology 2022Quote: ... 4×106 B cells per well were plated in 2 mL on 6-well plates that had been coated with RetroNectin (25 μg/mL, 4°C, overnight; #T100B, Takara), blocked with 2% BSA in PBS (1h ...
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bioRxiv - Neuroscience 2023Quote: ... and AAV1-syn-F14F15S-sTpEptTA_v2 were made by transfecting 8×15c plates (per virus) of HEK 293T cells using Xfect transfection reagent (Takara 631318). Briefly ...
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Induction of PARP7 Creates a Vulnerability for Growth Inhibition by RBN2397 in Prostate Cancer CellsbioRxiv - Cancer Biology 2023Quote: ... BBQ and FICZ as indicated at 37°C in 96-well plates for 5 days prior to measuring viability using WST reagent (Takara) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Fractionated CD34+ cells from Animals #2 and #3 were cultured overnight on RetroNectin-coated plates (Takara, T100B, Mountain View, CA) in X-VIVOTM 10 (Lonza ...
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bioRxiv - Neuroscience 2024Quote: ... and re-plated at a concentration of 2.5×105 cells/cm2 onto plates coated with 4 μg/mL iMatrix-511 (Takara Bio) in N2B27 medium supplemented with 10 μg/mL BDNF and 10 µg/mL Rock Inhibitor Y-27632 ...
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bioRxiv - Molecular Biology 2023Quote: Lentivirus was produced using a standard protocol64 from a 15 cm plate of 25M lenti-x 293T cells (Clontech #632180) via polyethylenimine (PEI ...
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bioRxiv - Cancer Biology 2024Quote: ... Meanwhile, untreated 6-well cell culture plates (Falcon, 351146) were coated with 15 µg/ml retronectin solution (Takara Bio, T100A) overnight at 4 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... approximately 20% confluent HEK293T cells seeded in 12-wells plates were transfected with 300ng ZNRF3 encoding plasmid and 50ng GFP expression plasmid (pEGFP-C1, Clontech) using Lipofectamine 2000 ...
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bioRxiv - Cell Biology 2024Quote: iPSCs SFCi55 and hESCs RUNX1-GFP were plated at 3 × 105 cells per a well of a 6 well plate and reverse transfected with 2 μg of DNA using the Xfect Transfection reagent (Clontech) and analyzed 2 days later.
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bioRxiv - Genetics 2024Quote: ... 0.2*106 mESCs were seeded in a well of a 12-well plate in 2iL and transduced the next day with 1ml of 5x concentrated (lenti-X, Clontech) and filtered viral supernatant with 8 ng/μl polybrene (Sigma Aldrich) ...
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bioRxiv - Plant Biology 2023Quote: ... the Y2HGold strain was transformed with the bait vector (pGBKT7-SR45) and then mated with strain Y187 containing an Arabidopsis cDNA library (Mate and Plate Library-Universal Arabidopsis, Clontech) (Stankovic et al. ...
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bioRxiv - Immunology 2019Quote: ... Viral titers were measured by a kit (Lenti-X p24 Rapid titer kit, #632200. Takara, Kusatsu, Japan). To suppress Mul1 expression ...