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Citations for Takara Bio :
401 - 450 of 532 citations for Chloroethane D5 98% 1000 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Genomes in the cell lysate were digested by 600U/mL of HindIII (Takara Bio, Shiga, Japan) for 4 hours at 37°C ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was directly loaded into a gravity column filled with 1 mL TALON resin (TaKaRa) pre-equilibrated with the binding buffer (20 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Cell Biology 2019Quote: ... The supernatant was pH’ed to 7.6 and mixed with 1 ml Talon Metal affinity resin (Clontech 635509) for 30 min ...
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bioRxiv - Microbiology 2019Quote: ... We added 5 μl of transformants (107/mL) on SD/–Leu/–Trp (Clontech, Mountain View, CA, USA) and SD/–Ade/– His/–Leu/–Trp (3 mM 3-AT ...
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bioRxiv - Microbiology 2019Quote: ... and the supernatant was applied to 1 mL of pre-washed TALON metal affinity resin (Takara Clontech) at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... and the supernatant was applied to 1 mL of pre-washed TALON metal affinity resin (Takara Clontech) at 4°C ...
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bioRxiv - Synthetic Biology 2021Quote: ... Concentrated protein was bound to 0.5–1 mL of His60 nickel or HisTalon cobalt resin (Takara Bio) for 0.5–1 hr at 4°C while rotating in a 10-mL Pierce disposable column (Thermo Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... 50% fresh RPMI medium supplemented with 20 U/ml IL-2 and spin-fected on RetroNectin (Takara)-coated plates at 3000 g at 32°C for 2 h ...
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bioRxiv - Cell Biology 2022Quote: ... cells were treated with the appropriate chemicals at the following concentrations: 1 µg/ml doxycycline (Clontech, 631311), 2.5 µM MG132 (APExBIO ...
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bioRxiv - Biochemistry 2019Quote: ... 500 µg protein at 1 mg/mL were prepared with Buffer A (Phosphoprotein Kit, Clontech, Cat#635626): 185 µL PC-3 lysate (pH 8.8) ...
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bioRxiv - Immunology 2019Quote: ... Retroviral supernatant was added to non-treated retronectin-coated (10 µg/ml) 6-well plates (Takara Bio) and spun for 30 min (1,200 x g ...
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bioRxiv - Bioengineering 2020Quote: The cleared lysate was poured on 5 mL packed Ni-NTA agarose (His60 Superflow, TaKaRa Bio Europe) gravity flow columns ...
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bioRxiv - Immunology 2021Quote: ... wild-type SARS-CoV-2 RBD was purified using a 5 mL HisTALON superflow cartridge (Takara Bio) followed by size exclusion chromatography using a Superdex 200 10/300 GL column pre-equilibrated in 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: ... 40 μg mL−1 5-Bromo-4-Chloro-3-Indolyl-beta-D-Galactosidase (X-gal, Takara Bio), and 500 μM isopropyl beta-D-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Immunology 2022Quote: ... The NTD domain was purified from clarified supernatants using 2 ml of cobalt resin (Takara Bio TALON), washing with 50 column volumes of 20 mM HEPES-HCl pH 8.0 and 150 mM NaCl and eluted with 600 mM imidazole ...
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bioRxiv - Cell Biology 2022Quote: ... cells were diluted to 25,000 cells/mL and dispensed into ICELL8 3’ DE chips (Takara Bio, CA) using an MSND device (Takara Bio) ...
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bioRxiv - Genetics 2023Quote: ... resuspend in 50 mM NaCitrate containing 0.25 mg/ml of boiled RNase A (2158, Takara, Kusatsu, Japan), at 37℃ for 2 h ...
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Comprehensive mutational analysis of the checkpoint signaling function of Rpa1/Ssb1 in fission yeastbioRxiv - Genetics 2023Quote: ... the supernatant was loaded onto a 5 ml column with prewashed Talon resin (Clontech Laboratories, Inc, CA). The column was washed three times with the low pH buffer (pH 6.3) ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was incubated overnight with 0.5 ml of Talon (immobilised metal affinity chromatography, IMAC) resin (Clontech) in the presence of 150 mM NaCl and 20 mM imidazole ...
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bioRxiv - Neuroscience 2024Quote: ... The preserved HEK medium was then spun down with 12.5 ml of lenti-X concentrator (Takara Bio), in an effort to capture the two distinct lentiviruses ...
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bioRxiv - Cell Biology 2020Quote: ... MC3T3-E1 and ATDC5 cells were infected with CRISPR lentivirus and selected with puromycin (4.5 μg/ml, Clontech) for 7 days ...
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bioRxiv - Genomics 2022Quote: Induction of the expression of Asmt by the addition of doxycycline (0.5 µg/mL; Cat# 631311, Takara Bio) for 24 h and total RNA was extracted using Trizol reagent ...
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bioRxiv - Microbiology 2022Quote: ... KSHV replication in iSLK/Bac16 cells was reactivated by a combination of 1 μg/ml doxycycline (DOX, Clontech) and 1 mM sodium butyrate (Bu ...
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X-ray mediated scintillation increases synaptic activity via Cerium-doped LSO and Channelrhodopsin-2bioRxiv - Neuroscience 2020Quote: ... showing a viral titer of >106 IFU/ml as measured by Lenti-X GoStix (Takara, Mountain View, CA). To develop stably expressing OptoXR-EYFP cells ...
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bioRxiv - Biochemistry 2022Quote: ... the cleavage mixture was applied to a gravity flow column packed with 8 mL TALON cobalt resin (Takara) equilibrated in AEX buffer ...
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bioRxiv - Cell Biology 2022Quote: ... Matings between the Y2HGold and Y187 strains carrying the appropriate constructs were performed in 0.5 mL of 2X YPDA in the presence of the corresponding antibiotics following manufacturer’s instructions (Clontech’s Matchmaker Gold Yeast Two-Hybrid System ...
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bioRxiv - Genetics 2022Quote: ... 0.1-0.2*106 mESCs were seeded per well in a 24-12-well plate in conventional ESC medium and transduced the next day with 0.25-0.5 ml of 10:1 concentrated (lenti-X, Clontech) supernatant with 8 ng/μl polybrene (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2022Quote: Nuclei were isolated from 1.5 × 109 DT40 cells and digested with 60 units/ml MNase (Takara Bio Inc.) in buffer A (15 mM Hepes-KOH ...
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bioRxiv - Cell Biology 2023Quote: ... cultures were split into two 2-ml cultures of which one was supplemented with 250 nM rapalog (Clontech). Mislocalisation of the target protein was verified by live-cell microscopy.
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bioRxiv - Immunology 2023Quote: Non-tissue culture treated 12-well plates were coated overnight at 4°C with 1 ml Retronectin (Takara) at 25 μg/ml in PBS ...
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bioRxiv - Genetics 2023Quote: ... and selection was performed on SD-Trp-Leu plates containing 200 ng/ml of Aureobasidin A (Clontech, 630466) according to the manufacturer’s instructions (Clontech) ...
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bioRxiv - Cancer Biology 2024Quote: ... 500 cells were collected in the center of a 1.5 ml centrifuge tube containing 4.75 μl SMART-seq reaction buffer (Takara) using a BD FACSymphony S6 (BD Bioscience) ...
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bioRxiv - Microbiology 2024Quote: ... The supernatants from the samples were added to 0.5 ml of a Talon metal affinity resin (Clontech, USA) equilibrated with binding buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were infected with concentrated lentivirus for 24 h in wells pre-coated with Retronectin (0.2 mg/ml; Takara) in expansion medium supplemented with protamine sulfate (10 μg/ml) ...
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bioRxiv - Developmental Biology 2019Quote: ... 200 ng of expression-ready Donor clone and acceptor Vector were recombined in a final volume of 10 µl for 15 minutes at 25 °C in a thermal cycler in the presence of Cre recombinase (0.2 units) and recombinase buffer supplemented with BSA (0.1 mg/ml) (Clontech #631614) according to Clontech manual PT3460-1 (now part of Takara Bio) ...
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bioRxiv - Genomics 2019Quote: ... We spun down the aliquots and re-suspended the pellets in 1 ml DroNc-seq resuspension buffer (1X PBS, 0.01% BSA, 0.16 U/μl RNase Inhibitor (Clontech/TaKaRa, #2313A)) for Tube A and 1 ml 10X Nuclei resuspension buffer (1X PBS ...
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bioRxiv - Immunology 2021Quote: ... Concentrated vectors were added at the indicated MOI (multiplicity of infection) in presence of retronectin at 10ug/ml (Takara). The plates were then centrifuged at 1,000 g for 1 h and incubated at 37°C during 3hrs ...
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bioRxiv - Biochemistry 2020Quote: ... The final supernatant was supplemented with NaCl to 150 mM and incubated with 3 mL His60 resin (Takara Biosciences) for 30 min ...
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bioRxiv - Microbiology 2020Quote: ... cells were cultivated for three days in growth medium supplemented with 20 μg/ml of puromycin (Takara Bio Inc.) for PVR1 KO or 40 μg/ml for PVR2 KO ...
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bioRxiv - Neuroscience 2021Quote: ... 10 μM ROCK inhibitor (Y-27632; Selleckchem, Cat. No. S1049) and 2 μg/mL doxycycline (Clontech, Cat. No. 631311). Media was changed daily during this stage.
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Panacea: a hyperpromiscuous antitoxin protein domain for the neutralisation of diverse toxin domainsbioRxiv - Microbiology 2021Quote: ... Filtered lysate was incubated with 1 mL of previously buffer equilibrated Ni-beads (His60 Ni Superflow Resin, TaKaRa, Japan) for 30 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... The culture was split into two 2-mL cultures of which one was supplemented with 250 nM rapalog (Clontech). Parasite growth was determined via flow cytometry over five days as described above ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant was clarified by centrifugation (292,055 g, 60 min, 4°C) and bound to 2 ml of TALON IMAC resin (Clontech) overnight with 10 rpm rotation in the presence of 20 mM imidazole and NaCl added up to 800 mM ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were washed twice with 20 mL of D-PBS (Nacalai Tesque) and resuspended in CELLBANKER 1 (Takara Bio) at 1 × 107 cells/mL ...
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bioRxiv - Molecular Biology 2022Quote: ... The BAC containing Idh was extracted from 200 ml bacterial cultures using the NucleoBond Xtra Midi kit (Takara Bio). The fluorescence in situ hybridization (FISH ...
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bioRxiv - Biochemistry 2021Quote: ... prepared as described above was mixed with imidazole (5 mM) and 0.5 mL of Talon superflow metal affinity resin (Clontech) that had been equilibrated with buffer (as above for magnetic agarose) ...
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bioRxiv - Molecular Biology 2021Quote: ... The insoluble material was removed by centrifugation at 150,000 ×g and the supernatant was added to 1 ml pure TALON resin (Clontech) and 20 mM imidazole and rock slowly overnight at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... His-tagged NTD domain constructs were purified from clarified supernatants using 2 ml of cobalt resin (Takara Bio TALON), washing with 50 column volumes of 20 mM HEPES-HCl pH 8.0 and 150 mM NaCl and eluted with 600 mM imidazole ...
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bioRxiv - Immunology 2021Quote: ... The supernatants were collected and applied to a column filled with 5 ml of TALON Metal Affinity resin (Takara) previously equilibrated with ice-cold PBS ...
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bioRxiv - Immunology 2021Quote: ... and transduced to NK-92MI cells in a 24-well plate coated with 0.5 µg/ml of RetroNectin diluted in PBS (Clontech). Two days later ...