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Citations for Takara Bio :
1 - 50 of 1429 citations for BFF 122 CAS 1152314 49 2 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... Japan) and cloned into a pEGFP-N1 vector that encodes EGFP [49] using an In-Fusion HD Cloning Kit (TaKaRa, Mountain View, CA). The mouse ...
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bioRxiv - Microbiology 2019Quote: ... Advantage 2 Polymerase (Takara Bio, Mountain View, CA), mM each dNTP ...
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bioRxiv - Molecular Biology 2021Quote: ... Pre-miR-122 or pre-miR-146a sequence cloned within pTRE-Tight-BI Vector (Clontech) in NheI and NotI sites or Bam HI and Hind III to make induced miR-122 (imiR-122 ...
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bioRxiv - Genomics 2019Quote: ... Retroviruses were packed using the EcoPack 2-293 cells (Clontech, Mountain View, CA) and infections were performed as described12 ...
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bioRxiv - Neuroscience 2020Quote: ... The cDNA was then amplified using the Advantage 2 PCR Enzyme System (Takara, Fremont, CA) for 6 cycles ...
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bioRxiv - Genomics 2019Quote: ... we used the miRNA quantifications from all brain libraries with all starting amounts using both batches (total of 99 libraries, 19 for the Clontech, NEXTflex ...
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bioRxiv - Developmental Biology 2024Quote: ... using the same paired read adapter oligonucleotides described by 99 were prepared from the ChIP and Input DNAs on an automated system (Apollo 342, Wafergen Biosystems/Takara). After a final PCR amplification step ...
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bioRxiv - Molecular Biology 2024Quote: ... Amplification was performed using the Advantage 2 Polymerase Mix (Clonetech, now Takara Bio USA, Mountain View, CA) and the following primers ...
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bioRxiv - Genomics 2019Quote: ... Long range PCR was performed using Advantage 2 Polymerase following the manufacturer’s protocol (Clontech Laboratories, Mountatin View CA).
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bioRxiv - Genomics 2021Quote: ... Long range PCR was performed using Advantage 2 Polymerase following the manufacturer’s protocol (Clontech Laboratories, Mountain View CA).
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bioRxiv - Biochemistry 2022Quote: ... and ligated into the multiple cloning site 2 (MCS2) of the pTRE3G-BI vector (Clontech, Mountain View, CA, USA), resulting in the construct pTRE3G-BI/GPR83-LgBiT ...
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bioRxiv - Cell Biology 2023Quote: ... Fractionated CD34+ cells from Animals #2 and #3 were cultured overnight on RetroNectin-coated plates (Takara, T100B, Mountain View, CA) in X-VIVOTM 10 (Lonza ...
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bioRxiv - Bioengineering 2020Quote: ... The lysate was loaded onto a prepared column with 2 mL TALON Metal Affinity Resin (Clontech Laboratories, Inc., Mountain View, CA). After being washed twice with 5 column volumes (CV ...
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bioRxiv - Immunology 2020Quote: Transferrin over-expression or knockdown vectors were constructed and HEK 293T cells (Conservation Genetics CAS Kunming Cell Bank, China) and EcoPack™ 2–293 cells (Clontech, USA) were used to package lentiviruses and retroviruses ...
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bioRxiv - Genetics 2023Quote: ... RNA from 2 retinae of a mouse was extracted using the NucleoSpin® RNA kits (Takara Bio USA, Inc., San Jose, CA). RNA sample concentration and quality was determined with NanoDrop Oneᶜ Microvolume UV-Vis Spectrophotometers (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... and Evl (isoform 2) were amplified by PCR from a NIH 3T3 cDNA library and ligated into suitable sites of pEGFP-C1 (Clontech, Palo Alto, CA). VASP cDNA was additionally inserted into the BglII and SalI sites of pmCherry (Addgene ID ...
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bioRxiv - Cell Biology 2022Quote: ... Transformants were selected on synthetic media containing yeast nitrogen base (YNB, Difco) supplemented with 2 % glucose and DropOut supplements lacking uracil (Clontech, Mountain View, CA). Single colonies were used to inoculate 5 mL liquid YNB media supplemented with 2 % glucose and DropOut supplements lacking uracil ...
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bioRxiv - Evolutionary Biology 2024Quote: ... adhaerens yeast 2-hybrid cDNA library was constructed using the Make Your Own “Mate & PlateTM” Library System (Takara Bio USA, Mountain View, CA), using whole animal total RNA extracted from approximately 30 animals using a Nucleospin RNA Plus Mini Kit (Macherey-Nagel ...
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bioRxiv - Neuroscience 2023Quote: ... were transiently cotransfected with a 2:1 mixture of Nav1.7-expressing plasmid8 and a pIRES2-ZsGreen bicistronic plasmid (Takara Bio, San Jose, CA) expressing ZsGreen and mouse FHF2B proteins.10 The same pIRES2-ZsGreen plasmid without FHF2 coding sequence served as the control ...
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bioRxiv - Microbiology 2021Quote: ... The knockout construct was developed through In-Fusion using the purified amplicons based on the manufacturer’s instructions with the insert to vector ratio of 2:1 (Takara Bio, Mountain View, CA, USA) and transformed into E ...
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bioRxiv - Bioengineering 2019Quote: KOD-Plus-Ver.2 DNA polymerase (Toyobo, Osaka, Japan) was used to amplify ldsDNAs (PCR amplicons) using the pEGFP-C1 (Clontech, Mountain View, CA, USA) plasmid as the template ...
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bioRxiv - Bioengineering 2024Quote: ... To synthesize cDNA, total RNA (8 µL, ca. 500 ng) was mixed with 2 µL of PrimeScript™ RT Master Mix (Takara Bio, Kusatsu, Japan) and incubated at 37°C for 15 min ...
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bioRxiv - Synthetic Biology 2023Quote: ... 2 µL of 2 mM dNTPs (Takara #4025), and 0.6 µL of 100% DMSO (NEB #12611P) ...
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bioRxiv - Synthetic Biology 2023Quote: ... 2 µL of 2 mM dNTPs (Takara #4025), and 0.6 µL of 100% DMSO (NEB #12611P) ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 (TaKaRa) and purified using ProbeQuant G-50 Micro Columns (GE Healthcare ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 (TaKaRa) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: DNA encoding the PX domain from the Qc SNARE Vam7 (Amino acyl residues 2-123) was amplified by PCR with CloneAMP HiFi PCR premix (Takara Bio USA, Mountain View, CA, USA). The amplified DNA fragment was cloned into BamHI and SalI digested pGST parallel1 vector (Sheffield et al.,1999 ...
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bioRxiv - Neuroscience 2021Quote: ... pDsRed-ER (Takara Bio, CA), RUSH-GPI-mApple ...
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bioRxiv - Neuroscience 2020Quote: ... pDsRed-ER (Takara Bio, CA), RUSH-GPI-mApple ...
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bioRxiv - Cell Biology 2021Quote: ... pEGFP (Clontech, Mountain View, CA), pEGFP2 and pEGFP3 ((Pack et al. ...
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bioRxiv - Cancer Biology 2019Quote: ... The cDNA synthesis and PCR used reagents from the SMARTer Ultra Low RNA kit from Illumina sequencing (Clontech, Mountain View, CA). After harvest from the C1 device ...
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bioRxiv - Immunology 2020Quote: ... pDsRed-mito was purchased from Clontech (Takara, Mountain View, CA, USA). Each construct was verified by sequencing.
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bioRxiv - Neuroscience 2023Quote: ... and eGFP (Clontech. CA, USA) in tsA201 cells using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 kit (Takara) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 (Takara, #6045) according to manufacturer’s protocols ...
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bioRxiv - Immunology 2022Quote: ... version 2 (Takara). Fastq files were generated from bcl files using BCL2FASTQ v2.17.1.14 with default parameters ...
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bioRxiv - Neuroscience 2022Quote: ... 2 (Takara Bio).
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bioRxiv - Molecular Biology 2023Quote: ... 2 (Takara Bio).
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bioRxiv - Evolutionary Biology 2019Quote: ... derived from pEGFP-C1 (Takara, CA). To validate the response of the CaMKII-KTR to intracellular activity of CaMKII ...
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bioRxiv - Cancer Biology 2019Quote: 293GP cells (Clontech, Mountain View, CA) were seeded to 70% confluence in DMEM medium containing 10% fetal bovine serum (FBS) ...
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bioRxiv - Microbiology 2021Quote: ... or Stellar (TaKaRa, Mountain View, CA) cells and purified using QIAprep spin and midi kits (Qiagen ...
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bioRxiv - Bioengineering 2023Quote: ... GP2-293 cell line (Takara, CA) was used as the viral packing cell line for plasmid encoding CAR construct ...
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bioRxiv - Cell Biology 2023Quote: ... or pEGFPC1 (Clontech, Mountain View, CA). Mouse Fcho2 was cloned into pCMV-Tag3B-myc or pmCherry-C1 ...
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bioRxiv - Bioengineering 2022Quote: ... Inc (Takara Bio, San Jose, CA). Converted cDNA products were next column purified and subsequently amplified using universal primers designed relative to an internal consensus sequence derived from analysis of 800 patient samples ...
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bioRxiv - Developmental Biology 2019Quote: ... 2 mM dithiothreitol (Clontech), 2 μM template switching oligo (Exiqon) ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 (Takara, Shiga, Japan) and 0.32 µM of each primer according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... from pTRE-Tight (Clontech, Mountain View, CA) to generate CSII-TRE-Tight-RfA ...
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bioRxiv - Cell Biology 2020Quote: ... WT ZAP-70 or C564R ZAP-70 was cloned into the pmCherry-C1 vector from Clontech (now Takara Bio; 632524; Mountain View, CA) using the NEBuilder high-fidelity DNA assembly cloning (NEB ...
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bioRxiv - Genomics 2021Quote: ... (Takara Bio USA, Inc. Mountain View, CA). Libraries were sequenced in-house using a NextSeq 500 High- Throughput Sequencing System (Illumina San Diego ...
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bioRxiv - Genomics 2020Quote: ... (Takara Bio USA, Inc, Mountain View, CA). Libraries were sequenced in-house using a NextSeq 500 High-Throughput Sequencing System (Illumina ...