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Citations for Takara Bio :
1 - 50 of 196 citations for Adenovirus Type 11 Particles Wild type since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... wild-type SAF-A was cloned into pEGFP-N1 (Clontech) and confirmed by DNA sequencing ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and wild type etoll-9 was cloned in pGBKT7-BD vector (Clontech). Each mutant etoll-AD was transformed in Y187 strain followed by selection on synthetic defined (SD ...
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bioRxiv - Genomics 2019Quote: ... Wild-type sequences were generated by PCR using human genomic DNA (Clontech) as a template ...
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bioRxiv - Neuroscience 2019Quote: ... replication-defective adenoviral vectors expressing dominant wild type FoxO3a were designed and purchased from TaKaRa Biotechnology (Dalian ...
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bioRxiv - Cell Biology 2022Quote: Full length wild type human RORβ (NM_006914.3) was subcloned into the pLPCX retroviral vector (Clontech) using XhoI and NotI restriction enzymes (NEB ...
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bioRxiv - Biochemistry 2024Quote: The Upf1-HD-His6 wild-type protein was first purified on a TALONTM resin (Clontech) equilibrated with buffer A200 ...
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bioRxiv - Neuroscience 2021Quote: ... Alix-YFP was obtained by subcloning wild type Alix cDNA into a pEYFP-N1 vector (Clontech). GFP-flag-Alix (GFP-Alix ...
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bioRxiv - Genetics 2022Quote: Full length wild-type or mutant BRC-1 sequences were cloned into plasmid pBridge (Takara Bio), transformed into yeast strain Y2HGold (Takara Bio ...
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bioRxiv - Plant Biology 2024Quote: ... The SKI2 cDNA sequence was amplified from wild type cDNA using CloneAmp HiFi PCR Premix (Takara) and cloned into the pPZP212 binary vector ...
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bioRxiv - Cell Biology 2021Quote: ... based on the manufacturer’s recommendation with wild type MST2 or MST2_delEDG subcloned in pLVX_TetOne-Puro (Clontech # 631849). Cells were cultured in presence of Doxycycline (Dox ...
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bioRxiv - Immunology 2021Quote: ... wild-type SARS-CoV-2 RBD was purified using a 5 mL HisTALON superflow cartridge (Takara Bio) followed by size exclusion chromatography using a Superdex 200 10/300 GL column pre-equilibrated in 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: Total RNA was isolated from wild-type or transiently transfected cells with MiniBEST Universal RNA Extraction Kit (Takara), an additional DNase I (NEB ...
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bioRxiv - Cell Biology 2021Quote: ... listed in table S2) and inserted the 2xFlag_2xStrep_MST2 constructs (wild type or del_EDG) in the inducible vector pLVX (Clontech, #631847).
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bioRxiv - Microbiology 2020Quote: ... The transgenes were inserted between the P and M gene of pNDV LaSota (LS) wild type or the L289A (15, 22, 23) mutant (NDV_LS/L289A) antigenomic cDNA by in-Fusion cloning (Clontech). The recombination products were transformed into NEB® Stable Competent E ...
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bioRxiv - Molecular Biology 2020Quote: ... the wild-type CARD14 insert with N-terminal 3xFLAG tag was cloned into pBApo-EFalpha Pur DNA (Takara Bio) whose EF-1α promoter was replaced by TRE3G promoter obtained from pTRE3G (Clontech) ...
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bioRxiv - Cell Biology 2019Quote: Human PDE4D5 wild type and PDE4D5-dN mutant were PCR amplified and cloned into pLVX-mCherry-N1 vector (Clontech) using Gibson assembly after vector digestion with XhoI and EcoRI ...
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TIPRL1 and its ATM-dependent phosphorylation promote radiotherapy resistance in head and neck cancerbioRxiv - Cancer Biology 2023Quote: ... Wild-type (WT) TIPRL1 cDNA was cloned into 3xFLAG pLenti6.4 using In-Fusion® HD Cloning Kit (Takara, #639650). Phosphomimic and non-phospho mutants (S265D and S265A ...
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bioRxiv - Cell Biology 2023Quote: ... procollagen type I (Takara #MK101), fibronectin (Takara #MK115) ...
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bioRxiv - Genetics 2020Quote: ... Full length wild type NHR-25 was tagged with EGFP at its N-terminus using pEGFP-C2 plasmid vector (Clontech); wild-type and mutant LIR-2s were N-terminally FLAG-tagged using the p3xFLAG-CMV-10 expression vector (SIGMA-Aldrich) ...
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bioRxiv - Neuroscience 2021Quote: ... a DNA fragment of the TkR86C coding region was amplified from cDNA from the Canton-S wild type strain by PCR (PrimeSTAR GXL, TaKaRa Clontech) with primers that had NotI and XbaI sites at 5’ and 3’ ends ...
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bioRxiv - Cancer Biology 2021Quote: ... The wild-type BTK construct was cloned in the base vector (Lenti-X Expression System Version EF1-a; Takara Bio). STable over-expression of BTK in ibrutinib-resistant DLBCL cells was performed by using lentiviruses expressing human wild-type BTK as described previously.[15]
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bioRxiv - Biochemistry 2022Quote: ... wild type and mutant constructs of full-length PKD1 were cloned into the EGFP-C1 and mCherry-C1 vectors (Clontech), resulting in N-terminally labelled fusion proteins ...
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bioRxiv - Biochemistry 2019Quote: ... Cells were transiently transfected either with GluK3EM or co-tansfected with Wild type/mutant receptors along with GFP expressing plasmid (2 µg/dish) using Xfect reagent (Clontech). Currents were recorded from medium sized cells expressing a moderate level of fluorescence from either the fused EGFP in case of GluK3EM or co-expressed EGFP and having a capacitance of ∼5-6 pF at 48-60 hours post transfection ...
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bioRxiv - Plant Biology 2023Quote: ... Total RNA was isolated from 14-day-old thalli of the wild type and Mpacl5 mutants grown on the half-strength B5 agar medium using NucleoSpin RNA Plant (Takara). The sequence libraries were generated by TruSeq RNA Sample Prep Kit (Illumina ...
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bioRxiv - Neuroscience 2021Quote: ... a DNA fragment of the TkR86C coding region was amplified from cDNA from the Canton-S wild type strain by PCR (PrimeSTAR GXL, TaKaRa Clontech) with primers that had NotI and XbaI sites at 5’ and 3’ ends ...
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bioRxiv - Genetics 2022Quote: ... the ptetO7-ALA1 strain was transformed with a vector (pAG425) to express wild-type or mutant AARS1 and grown on media lacking leucine (DO Supplement -Leu, Takara Bio). One colony was picked ...
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bioRxiv - Neuroscience 2022Quote: ... The full-length wild-type TRPV4 was subcloned into the PLVX-IRES-mCherry vector to generate TRPV4WT (Clontech, Catalog No. 631237). To generate the TRPV4FeRIC construct ...
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bioRxiv - Neuroscience 2022Quote: Total RNA was isolated from 8-week-old wild-type or Inka2-/- mice forebrains using the RNAiso Plus reagents (Takara Bio). PolyA+ mRNA was extracted from total RNA using Oligotex TM-dT30 mRNA Purification Kit (Takara Bio) ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293T expressing either iRhom2-1-374 or wild-type iRhom2 under TET-inducible cells were generated by lentiviral infection using gene cloned into pLVX-TetOne vector (Clontech, #631849). Methodology is similar to retroviral transduction ...
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bioRxiv - Biophysics 2023Quote: Retroviral constructs encoding HA3-CXCR4 wild-type or designed variants were generated using the In-Fusion HD Cloning Kit (Takara, ref: 638933). Sequences of interest from the expression constructs mentioned above were amplified by high-fidelity PCR (CloneAmp HiFi PCR Premix ...
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bioRxiv - Microbiology 2023Quote: The whole Gag sequence was amplified from wild-type Gag plasmid by PCR and cloned into pGEX6P1 and pmCherry-C1 (Takara Bio Inc.). Fragments of MAp17 (a.a ...
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bioRxiv - Microbiology 2022Quote: ... The recombinant adenovirus was produced using an Adenovirus Dual Expression Kit (Takara Bio) in accordance with the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2023Quote: ... was used as the wild-type (WT) strain for genetic manipulations. Chemically competent Escherichia coli (E. coli) DH5α and HST08 (Stellar Competent Cells, TaKaRa Bio Inc., Kusatsu, Shiga, Japan) were used as the host strains for molecular cloning.
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bioRxiv - Developmental Biology 2023Quote: ... Plasma Gla-type osteocalcin was analyzed by enzyme immunoassay (#MK111, Takara, Japan). The absorbance was read at 450 nm using a microplate reader (Bio-Tek ...
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bioRxiv - Cancer Biology 2021Quote: ... The wilde-type (WT) hTERT-immortalized retinal pigment epithelium (hTERT-RPE1, Clontech, Palo Alto, CA) and RPE1–MYCN-ER were cultured in DMEM/F-12 HEPES ...
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bioRxiv - Cell Biology 2020Quote: ... Adenovirus expressing human SPARC was constructed using Adeno-X expression system (Clontech) as described before 30,31 ...
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bioRxiv - Cell Biology 2023Quote: Procollagen Type 1 C-peptide (PIP) was measured according to the manufacturer’s instructions (Takara, Shiga, Japan). For evaluation of PIP ...
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bioRxiv - Immunology 2024Quote: ... HEK 293T cells were sourced from the American Type Culture Collection (ATCC) and GP2-293 from Takara Bio ...
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bioRxiv - Plant Biology 2020Quote: ... and used to replace the GTTT-type sgRNA in pENTR4:sgRNA4 using In-Fusion HD Enzyme Premix (Clontech), resulting in the entry vector pENTR4:sgRNA4-NG.
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bioRxiv - Neuroscience 2020Quote: ... cDNA of Human Phosphodiesterase type 10 (hPDE10) was amplified by conventional nested PCR using human brain cDNA (Clontech). cDNAs template encoding wild-type and D674A mutant form of hPDE10 catalytic domain (CD ...
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bioRxiv - Plant Biology 2021Quote: ... The two types of plasmids were co-transformed into yeast strain EGY48 according to the Yeast Protocols Handbook (Clontech) as described previously(Lin et al. ...
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bioRxiv - Molecular Biology 2024Quote: ... and the indicated type and amount of RNase Inhibitor (no RNAse inhibitor, 0.003 μl RNase Inhibitor (40 U/μl, Cat. 2313B, TaKaRa), SEQURNA Thermostable RNase inhibitor (Cat ...
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bioRxiv - Neuroscience 2021Quote: ChIP-seq libraries were generated from ChIP-DNA using a custom Illumina library type on an automated system (Apollo 342, Wafergen Biosystems/Takara). ChIP-seq libraries were sequenced on Illumina NextSeq 500 as single-end 75bp reads (Active Motif).
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bioRxiv - Molecular Biology 2021Quote: Secretion in PIP in NHLFs treated with MRG-201 or MRG-229 was conducted by collecting the supernatant of treated cells and performing procollagen type I PIP ELISA (Takara).
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bioRxiv - Genetics 2021Quote: ... full-length cDNA encoding MELK was inserted between BamHI and NotI sites of three types of retroviral plasmids including the pRetroX-Tight-puro (Clontech), pRetroX-Tight-GFP-puro (containing N-terminal GFP ...
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bioRxiv - Immunology 2021Quote: To construct NPM1 lentivector the mutant NPM1 cDNA (mutation type A, TCTG duplication) was cloned into the pLVX-EF1α-IRES-ZsGreen vector (Clontech) using EcoRI and BamHI restriction sites.
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bioRxiv - Molecular Biology 2020Quote: Adenovirus vectors were generated with the Adeno-X Adenoviral System 3 following manufacturer’s instructions (Takara, #632267). Briefly ...
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bioRxiv - Molecular Biology 2022Quote: ... The titer of adenovirus was examined using an Adeno-XTM Rapid Titer Kit (Takara Bio USA) and determined to be 3.4 × 1011 ifu/ml ...
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bioRxiv - Cancer Biology 2019Quote: ... The BLRP-tagged wild-type and pBox mutant ERα cDNAs were cloned into a retrovirus-based Tet-On expression vector pRetroX-Tight-Pur (Clontech #632104) 19 ...
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bioRxiv - Neuroscience 2021Quote: ... three types of the GPR151 CDS-related inserts were sub-cloned into FUGW vector using In-Fusion Cloning kit (Takara, 638910). For control experimental sets ...