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Citations for Takara Bio :
51 - 100 of 427 citations for ADAM Metallopeptidase Domain 7 ADAM7 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Point mutants of the microtubule-binding domain were created using site-directed mutagenesis using PrimeSTAR Max DNA polymerase (Takara Bio).
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bioRxiv - Genetics 2019Quote: ... The entire open reading frame for the protein Reg1 and its derivative Reg1-P231L was expressed as a fusion to the Gal4 activation domain in the plasmid pACT2 (Clontech).
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bioRxiv - Plant Biology 2022Quote: ... The CWLP coding region without signal peptide (SP) was cloned in frame downstream of the GAL4 DNA-binding domain in the bait vector pGBKT7 (Clontech) and introduced into the yeast strain AH109 (Clontech) ...
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bioRxiv - Microbiology 2023Quote: ... Fragments encoding the CD45 extracellular domain (ECD) and transmembrane region were amplified by PCR and cloned into pEGFP-N1 (Clontech) using the EcoRI and BamHI restriction sites ...
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bioRxiv - Immunology 2022Quote: ... Mate & Plate™ cDNA Library in a pGADT7 vector with the GAL4 DNA-activation domain (AD) and pre-transformed into yeast strain Y187 was acquired from Takara Bio ...
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bioRxiv - Evolutionary Biology 2023Quote: ... A total of 500 ng of sheared DNAs was input for methyl-CpG binding domain (MBD) enrichment using the EpiXplore Methylated DNA Enrichment Kit (Clontech) according to the manufacturer’s instruction ...
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bioRxiv - Plant Biology 2023Quote: ... The LexA fusion constructs (bait) and the activation domain fusion constructs (prey) were co-transformed into yeast strain EGY48[p8op-lacZ] (CLONTECH). Clones containing both constructs were selected on medium lacking His ...
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bioRxiv - Plant Biology 2023Quote: ... pGADT7 plasmids encoding the effector domain of different ZiF were co-transformed into chemically competent Y2HGold cells (Takara Bio, USA) with a pGBKT7 plasmid encoding OsExo70F3 in using a Frozen-EZ Yeast Transformation Kit (Zymo research).
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bioRxiv - Molecular Biology 2024Quote: The RBD-DsRed plasmid was created by PCR cloning the HB domain of RNAse H1 into the pDsRed-Express-C1 vector (Clontech), following the previously described method [32].
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bioRxiv - Plant Biology 2024Quote: ... the full-length ORF of PpGL2 was fused with the GAL4 transcriptional activation domain in the pGADT7 vector (Clontech, Japan), and then co-transformed with the pHIS2 cis reporter vector (containing triple tandem CAAATTG repeat sequence ...
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bioRxiv - Cell Biology 2020Quote: The cDNA coding sequences of the first and second cytoplasmic loop domain of human TMEM39A were cloned into the pGBKT7 vector and screened against a normalized universal human cDNA library (Clontech, 630481), following instruction of the Matchmaker® Gold Yeast Two-Hybrid System (Clontech ...
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bioRxiv - Biochemistry 2019Quote: ... A native poly-histidine region within the Spir-KIND domain is sufficient for binding of these constructs to TALON® resin (Clontech).
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bioRxiv - Plant Biology 2020Quote: ... and pGBKT7 (DNA-binding domain vector) by either restriction enzyme-based cloning or In-Fusion HD Cloning method (638910In-Fusion® HD Cloning Plus Takara). Primer sequences used to generate Y2H and Y3H constructs are listed in Supplemental Table 4 ...
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bioRxiv - Genetics 2020Quote: The cDNA coding sequence of the C-terminal domain of human TMEM132D was cloned into the pGBKT7 vector and screened with a normalized universal human cDNA library (Clontech, 630481) in pGADT7 Vector ...
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bioRxiv - Biochemistry 2022Quote: ... in the bait vector pGBKT7 or were fused to the GAL4 activation domain (AD) in the prey vector pGADT7 (Clontech Laboratories). The yeast strain Y2H Gold (Clontech Laboratories ...
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Plant pathogens convergently evolved to counteract redundant nodes of an NLR immune receptor networkbioRxiv - Plant Biology 2021Quote: ... NRC3 and NRC4 CC and NB-ARC domain truncations and ran a yeast two-hybrid experiment using the Matchmaker Gold system (Takara Bio USA). Plasmid DNA encoding AVRcap1b (negative control ...
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bioRxiv - Plant Biology 2020Quote: ... DBD) were transformed into Y2H Gold and prey constructs (GAL4 activation domain, AD) into Y187 strains by LiAc method (Takara Bio USA). Haploid strains were mated to produce diploid strains to test for interactions ...
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bioRxiv - Cell Biology 2021Quote: The SYP121 DNA (obtained from Riken) was amplified from cDNA with primers excluding the transmembrane domain and cloned into the pGADT7 vector (Clontech Laboratories, Inc.). All other exocyst constructs used in the study have been previously described [38,48,73](Hála et al. ...
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bioRxiv - Biochemistry 2022Quote: ... S domains and variants were purified similar to S protein using nickel resin (10 mL/L, His60 Ni2+ superflow resin, Takara cat# 635664) with wash buffer (25 mM MES ...
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bioRxiv - Plant Biology 2023Quote: ... The coding region of PIF4 was amplified and cloned into the yeast GAL4 activation domain (GAL4 AD) of pGADT7-Rec2 (Clontech, CA, USA) prey vector ...
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bioRxiv - Immunology 2024Quote: ... genes encoding the variable regions of C7 and C74 heavy chains were cloned into a pMN vector with a human CH1 domain and a C-terminal His-tag using In-Fusion cloning system (Takara Bio #639649). The full light chains of C7 and C74 were cloned into the pMN vector without any purification tag using the same method ...
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bioRxiv - Molecular Biology 2024Quote: ... HepG2-NTCP and Huh-7 cells with RNAi plus (TaKaRa, Japan) according to the manufacture’s protocol ...
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The Ets protein Pointed P1 represses Asense expression in type II neuroblasts by activating TaillessbioRxiv - Developmental Biology 2021Quote: ... 1-298) (EnR) or the Ets domain of PntP1 were amplified by CloneAmp HiFi PCR Premix (Catalog# 639298, Takara Bio., Mountain View, CA) from genomic DNAs of the UAS-PntP1 line ...
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bioRxiv - Cell Biology 2020Quote: A lentiviral plasmid encoding a transcriptional reporter for CREB activity was generated by PCR amplification of a 2xCRE promoter-driven GFP N-terminally tagged with the ProteoTuner destabilization domain (CRE-DD-GFP, Takara Bio, Cat #631085) and Gibson cloning into the FUGW lenti-vector backbone (Addgene ...
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bioRxiv - Plant Biology 2022Quote: ... bait proteins were cloned into the pGBKT7 vector which encodes the GAL4 DNA binding domain and then transformed into the Y2H Gold strain (Clontech, Mountain View, California) and plated on SD/- Trp to select for positive transformants ...
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bioRxiv - Cell Biology 2023Quote: We used an hESC line that transiently expresses the C-terminal region (catalytic domain) of histone demethylase JMJD3 in a doxycycline (DOX, Takara Bio Inc, Japan) inducible manner (12 ...
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bioRxiv - Neuroscience 2023Quote: ... 7 million Lenti-X HEK-293T cells (Takara Bio, cat. no. 632180) were seeded in 9 mL DMEM (Gibco ...
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bioRxiv - Biochemistry 2020Quote: Site-directed mutagenesis H >A of the HPGG motif of the Cyt-b5 domain was either performed by Genescript (N. benthamiana) or using In-Fusion® HD cloning kit (Takara Bio, Kusatsu, Japan) for Synechocystis after amplification using two mutagenic complementary primers for amplifying pTHT2031-Ot5H46A-S and pTHT2031-Ot10H20A-S from pTHT2031-Ot5-S and pTHT2031-Ot10-S ...
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bioRxiv - Neuroscience 2023Quote: ... with a HA tag and deletion mutants of Lag3 D1 domain with a myc tag were constructed by PCR with In-Fusion HD Cloning Plus systems (Takara; Bio Inc., Otsu, Japan) using the CloneAmp™ HiFi PCR Premix (a high-fidelity PCR polymerase included with all In-Fusion HD Cloning Plus Systems) ...
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bioRxiv - Microbiology 2020Quote: ... A mouse 7-day embryo cDNA Library (CATALOG No. 630478; Clontech Laboratories, Inc.) was used to identify host interaction proteins of Mtb PknG through yeast two-hybrid assay ...
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bioRxiv - Neuroscience 2021Quote: ... transfected at 5-7 DIV with the plasmid peGFP-N1 (Clontech, Mountain View, CA) using lipofectamine 2000 (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2019Quote: ... 15 pmol of siRNA is reverse transfected to 7×104 HeLa-Tetoff cells (Clontech) in a 12 well plate using 1.6 µl RNAiMAX (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: 7 μg of lentiviral vectors was mixed with Lenti-XTM packaging single shots (Clontech) in a final volume of 600μl for 15min before the transfection mix were added to 4 million of Lenti-X TM 293T cells seeded on a 10 cm2 tissue culture dish ...
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bioRxiv - Immunology 2023Quote: ... the non-tissue culture 24-well plates were coated with 7 μg/ml RetroNectin (TAKARA) for 3 hours at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... plasmids respectively using primers HindIII_ZIKV_sfRNA and XbaI_GFP (Supplementary table 7) and PrimeSTAR GXL Polymerase (Takara, Japan). Cycling conditions were as follows ...
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bioRxiv - Biophysics 2021Quote: ... The 7-kb band was excised and recycled using Agarose Gel DNA Extraction Kit (TaKaRa Bio). Concurrently ...
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bioRxiv - Cell Biology 2022Quote: MCF-7 and T47D cells were harvested and total RNA was extracted with RNA Trizol (TAKARA) following the manufacturer’s recommendations ...
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bioRxiv - Immunology 2021Quote: ... A2058 and MDST8 as previously described (7) with small changes for adherent cell lines omitting RetroNectin (Clontech). LCL-1 was transduced with single minigenes ...
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bioRxiv - Neuroscience 2023Quote: Neurons were transfected at day in vitro (DIV) 7 using CalPhos Mammalian Transfection Kit (Takara Bio, 631312). Transfection solution was prepared by adding 1.5 µg DNA per construct (3 µg in total ...
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bioRxiv - Molecular Biology 2020Quote: ... cDNA was amplified by PCR in separate 25 μL reactions each containing 2 μL of cDNA for typically 7-9 cycles using 0.5 μL (2.5 u) LA Taq (Takara) with P5-3’ and miRCat-PCR-2 oligos (IDT ...
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bioRxiv - Biochemistry 2022Quote: shTnsB mutants (Fig. 7) were generated in the pHelper vector using the In-Fusion cloning kit from Takara and primers containing the desired point mutations ...
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bioRxiv - Cell Biology 2024Quote: ... 7 μg of lentiviral vectors was mixed with a Lenti-X packaging (VSV-G) single shots tube (Clontech) in a final volume of 600 μl for 15 min before the transfection mix was added to subconfluent (80-90% ...
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bioRxiv - Genomics 2020Quote: The amplified library was then recombined with pCMV FAS wt minigene exon 5-6-7 (Förch et al., 2000) using the In-Fusion HD Cloning kit (639649, Clontech) in a 1:8 vector:insert optimized ratio and transformed into Stellar competent cells (636766 ...
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bioRxiv - Immunology 2019Quote: ... Rab 5 and Rab 7 cDNA (a gift of M. Sandor) and human CD81 cDNA (Open Biosystems) were cloned into pmCherry-N1 vector (Clontech). Raji/DC-SIGN cells were electroporated with 1 µg of indicated plasmid using the Eppendorf Multiporator in iso-osmolar electroporation buffer using a 90 µs ...
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bioRxiv - Biochemistry 2022Quote: ... 127926 and 127924). shTnsB mutants (Fig. 7) were generated in the pHelper vector using the In-Fusion cloning kit from Takara and primers containing the desired point mutations ...
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bioRxiv - Immunology 2022Quote: ... Lentiviral transduction of murine alveolar macrophages was performed for 7 days in the presence of 5 μg/cm2 RetroNectin (Takara). Stable gene expression was confirmed by GFP signals using a BZ-X710 (KEYENCE ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 × 106 HEK293T cells were transfected with 7 μg of Env expressor and 1 μg of a green fluorescent protein (GFP) expressor (pIRES2-EGFP; Clontech) with the calcium phosphate method ...
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bioRxiv - Molecular Biology 2022Quote: ... ceranae-inoculated workers’ midguts at 7 dpi and 10 dpi were respectively isolated using RNA Extraction Kit (TaKaRa company, Dalian, China). cDNA was synthesized through reverse transcription with oligo dT primer and used as templates for qPCR assay ...
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bioRxiv - Molecular Biology 2019Quote: The MCF-7 cell line (ATCC, Manassas, VA, USA) was stably transfected with peGFP-C1 vector (Clontech, Mountain View, California, USA) containing the GFP-Rab27b fusion protein ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were cotransfected with hSyn-DsRed and the indicated construct on DIV 7 or DIV 19 (for older neurons) with 1 μg total purified plasmid DNA via CalPhos Mammalian Transfection Kit (Takara Bio) according to the manufacturer’s protocol.