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Citations for Takara Bio :
151 - 200 of 542 citations for 9 10 12 13 tetrahydroxyoctadecanoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... For binding studies the 6xHis tag were removed from some Fabs by treatment with PreScission protease (MolBioTech; ThermoScientific) and the protein repurified on cobalt-nitrilotriacetic acid (Co-NTA) agarose (Clontech) followed by gel filtration chromatography on Superdex 200 (GE Healthcare ...
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bioRxiv - Cell Biology 2021Quote: ... CPE 451 mCherry constructed by Gibson assembly by sub-cloning the 451 amino acids of CPE (without the Amphipathic Helix) into pmCherry-N1 (Clontech) vector using XhoI and BamHI restriction sites.As control vectors we used pEGFP-N3 and pmCherry-N1.All constructs were sequenced to confirm the fidelity of the process ...
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bioRxiv - Neuroscience 2023Quote: ... inserting an in-frame 15bp flexible linker sequence (encoding the amino acids GGGGA) followed by EGFP at the C-terminus using Infusion cloning (Clontech). For the Tg(NBT:ap2s1-gfp ...
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Molecular basis of proteolytic cleavage regulation by the extracellular matrix receptor dystroglycanbioRxiv - Biochemistry 2024Quote: ... A Flag tag x3 was inserted between amino acids 489 and 490 and a HA tag was inserted between amino acids 724 and 725 using In-Fusion Cloning Mix (Takara).
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bioRxiv - Plant Biology 2022Quote: ... 10 µl of RNA extraction mixture was then added to 1 µl of 10 mM dNTPs (Takara Catalog # 4030) and 100 mM of oligo-dT(18 ...
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bioRxiv - Neuroscience 2020Quote: ... The reaction was performed in a final volume of 12 μL using the Premix Ex Taq Probe based qPCR assay (Takara Biotechnology, ref. RR390A). All reactions were run in duplicate ...
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bioRxiv - Molecular Biology 2023Quote: ... An RNA amount equivalent to 12 ng was used to prepare the sequencing library using SMARTer® smRNA-Seq Kit for Illumina® (Takara 635031) according to the kit manual till cDNA synthesis ...
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bioRxiv - Bioengineering 2023Quote: ... and 12 ng of total RNA was reverse-transcribed to generate cDNA using PrimeScript RT Master Mix (Perfect Real Time; TaKaRa Bio, Shiga, Japan) after cell sorting ...
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bioRxiv - Neuroscience 2023Quote: ... The qPCR reaction was performed on 96-well plates in a final volume of 12 μL using the Premix Ex Taq Probe based qPCR assay (Takara Biotechnology, ref. RR390A). All reactions were run in duplicate on a StepOnePlus Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Genomics 2023Quote: ... 1 μl of 5’ linker (10 μM) was ligated with 10 μl of Mighty mix (DNA Ligation Kit
, catalog num. 6023, Takara) to 4 μl of the sample coming from the previous step ... -
bioRxiv - Cell Biology 2019Quote: ... 10 μM a/c heterodimerizer (Clontech, cat#635057) was added to the medium (the same volume of EtOH was added to the control group) ...
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bioRxiv - Synthetic Biology 2019Quote: ... supplemented with 10% FBS (fetal bovine serum, Clontech) and 1% P/S (Penicillin-Streptomycin ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 10% tetracycline-free FBS (631106, Clontech, [Conda Laboratories ...
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bioRxiv - Plant Biology 2021Quote: ... using 10 μL SYBR Premix Ex Taq (Takara), 2 μL of 2-fold diluted cDNA ...
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bioRxiv - Cancer Biology 2022Quote: ... supplemented with 10% tetracycline-free FBS (Takara #631107) and penicillin-streptomycin (Gibco #15140122).
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MEK inhibitor resistance in lung cancer cells associated with addiction to sustained ERK suppressionbioRxiv - Cancer Biology 2022Quote: ... supplemented with 10% tetracycline-free FBS (Clontech, 631101) and Pen/Strep (Gibco ...
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bioRxiv - Microbiology 2021Quote: ... and RNA inhibitor (TaKaRa 2313; 10 units/reaction), Oligo-dT (IDT ...
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bioRxiv - Cancer Biology 2021Quote: ... and maintained in 10% tetracycline-free FBS (Clontech).
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bioRxiv - Molecular Biology 2019Quote: ... supplemented with 10% fetal bovine serum (FBS) (Takara), 1% MEM Non-Essential Amino Acids Solution (NEAA 100X ...
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bioRxiv - Neuroscience 2019Quote: ... supplemented with 10% foetal bovine serum (ClonTech, 631107) and 1% L-glutamine (Sigma ...
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bioRxiv - Synthetic Biology 2020Quote: ... supplemented with 10% FBS (fetal bovine serum, Clontech) and 1% P/S (Penicillin-Streptomycin ...
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bioRxiv - Synthetic Biology 2020Quote: ... supplemented with 10% Tet System Approved FBS (ClonTech), 100 U/ml penicillin ...
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bioRxiv - Neuroscience 2021Quote: ... supplemented with 10% foetal bovine serum (ClonTech, 631107), 1% L-glutamine (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... supplemented with 10% fetal bovine serum (FBS, Clontech) in a humidified atmosphere of 5% CO2 at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... supplemented with 10% fetal bovine serum (FBS, Clontech) in a humidified atmosphere of 5% CO2 at 37°C and transfected after 24 hours with the pX459-gRNADicerNterm-Cas9-2A-Puro plasmid and the Leftarm-FlagHAGFP-RightarmDICER donor plasmids at the ratio of 1 to 1 (6 micrograms plasmids in total ...
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bioRxiv - Cell Biology 2019Quote: ... supplemented with 10% Tet system approved FBS (Clontech) and 100 µg/ml geneticin ...
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bioRxiv - Genomics 2021Quote: ... 11 µL of 10 mM dNTP (Takara, #639125), 13.75 µL of ultra-pure water and 2.75 µL of RNase Inhibitor (Fisher ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with 10% fetal bovine serum (FBS) (Takara) in a humidified atmosphere of 5% CO2 at 37°C ...
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bioRxiv - Biochemistry 2021Quote: ... 10 μL of SYBR Premix Ex-Taq (TaKaRa), and 7.2 μL of H2O ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with 10% FBS (fetal bovine serum, Clontech) and 1% P/S (Penicillin-Streptomycin ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with 10% FBS (fetal bovine serum, Clontech) and 1% P/S (Penicillin-Streptomycin ...
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bioRxiv - Neuroscience 2022Quote: ... supplemented with 10% FBS (Clontech, Mountain View, CA), 50 U/mL penicillin ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% fetal bovine serum (FBS) (Clontech) and antibiotics ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% fetal bovine serum (FBS) (Clontech), human interleukin-2 (IL-2 ...
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bioRxiv - Immunology 2023Quote: ... and 10 μg/ml RetroNectin (Takara, Cat# T100A). Then ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 10% fetal bovine serum (FBS, Clontech) in a humidified atmosphere with 5% CO2 at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 10% fetal bovine serum (FBS, Clontech) and 50 μg/ml gentamicin (Life Technologies) ...
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bioRxiv - Synthetic Biology 2021Quote: ... which contains a six-amino acid His-tag for the creation of a N-terminal fusion using the In-Fusion HD cloning kit (Clontech, USA).
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bioRxiv - Molecular Biology 2021Quote: ... two to three amino acids in GFP-SP-BAP were replaced by alanine for each construct using the CloneAmp HiFi PCR Premix (Takara Bio) according to the manufacturer.
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bioRxiv - Biochemistry 2021Quote: ... Add 25 mL 200 g/L glucose and 25 mL 20 g/L amino acid drop-out mix (Clontech. Inc., Japan) solution to prepare the medium] ...
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bioRxiv - Neuroscience 2022Quote: ... Pak1 fragment (amino acids 270–521) was PCR-amplified from pCMV6M-Pak1 and subcloned into pCold-Pros2 vector (Takara Bio Inc.) to generate pCold-Pros2-Pak1cat ...
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bioRxiv - Immunology 2023Quote: ... The expression vectors for the S variants with multiple amino acid changes or deletions were generated using the In-Fusion HD cloning kit (Takara Bio). The pcDNA3.1-hACE2 used to express human ACE2 (hACE2 ...
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bioRxiv - Neuroscience 2023Quote: ... lysates were centrifuged at 31,000 x g for 45 min at 4°C and the supernatant was mixed with nickel-nitrilotriacetic acid (Ni-NTA) beads (Takara, 635653) at 4°C for 2 h ...
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bioRxiv - Genomics 2021Quote: ... 10 ng of cfDNA was dephosphorylated in a 10-μL reaction containing 2.5 μL of 10× TACS buffer and 1 μL of shrimp alkaline phosphatase (Takara Bio Inc.) at 37 °C for 15 min ...
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bioRxiv - Microbiology 2022Quote: ... RNA samples with sufficient material (10 pg–10 ng) were passed to whole-transcriptome library preparation using the SMART-Seq v4 PLUS Kit (Takara Bio) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... RNA samples with sufficient material (10 pg–10 ng) were passed to whole-transcriptome library preparation using the SMART-Seq v4 PLUS Kit (Takara Bio) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... 10 U ml−1 of HRV- 3C protease (Takara) were added to the Ni-NTA eluted protein to remove the oligohistidine- GST-tag during dialysis against 3 L of dialysis buffer I (25 mM Tris-HCl ...
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bioRxiv - Synthetic Biology 2019Quote: ... 10 nM of the chemical dimerizer AP20187 (ClonTech Laboratories) or left unstimulated ...
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bioRxiv - Microbiology 2019Quote: ... containing 10% Tetracycline-free fetal bovine serum complex (Clontech) and 1% penicillin-streptomycin (100 U penicillin/mL ...
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bioRxiv - Plant Biology 2022Quote: ... 10 μL of SYBR Premix Ex TaqTM II (Takara), and 0.4μL of reference dye ...