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• Alternative mRNA splicing controls the functions of the histone H3K27 demethylase UTX/KDM6A

  Omid Fotouhi, et al., bioRxiv - Biochemistry 2023

  Quote: ... GFP (JL-8, Clontech) or Vinculin (7F9 ...
• TRPC6 is a mechanosensitive channel essential for ultrasound neuromodulation in mammalian brain

  Yumi Matsushita, et al., bioRxiv - Neuroscience 2024

  Quote: ... and at 72°C for 30 sec in TaKaRa Thermal Cyclar Dice Touch (Takara).
• Linear motif specificity in signaling through p38α and ERK2 mitogen-activated protein kinases

  Jaylissa Torres Robles, et al., bioRxiv - Cell Biology 2022

  Quote: ... GFP (JL-8, Clontech, #632381). Secondary antibodies used included ...
• Oncogenic GNAS uses PKA-dependent and independent mechanisms to induce cell proliferation in human pancreatic ductal and acinar organoids

  Ridhdhi Desai, Senthil Muthuswamy, bioRxiv - Cancer Biology 2023

  Quote: ... GFP (632381,JL-8, Takara), p-VASP (3111 ...
• IFITM3 blocks influenza virus entry by sorting lipids and stabilizing hemifusion

  Steffen Klein, et al., bioRxiv - Molecular Biology 2023

  Quote: ... 8 µl Cloning Enhancer (Takara) was added to 20 µl of the PCR product and incubated for 15 min at 37 °C on a thermocycler ...
• Mar1, an HMG-box protein, regulates n-alkane adsorption and cell morphology of the dimorphic yeast Yarrowia lipolytica

  Chiaki Kimura-Ishimaru, et al., bioRxiv - Microbiology 2024

  Quote: ... Monoclonal Antibody (JL-8) (Clontech). Anti-mouse IgG was used as a secondary antibody.
• Polyubiquitin-dependent recruitment of NEMO/IKKγ into T cell receptor signaling microclusters

  Elizabeth A. DeRiso, et al., bioRxiv - Immunology 2019

  Quote: ... GFP (JL-8; RRID:AB_10013427) from Takara Bio (Kusatsu ...
• Pathways controlling neurotoxicity and proteostasis in mitochondrial complex I deficiency

  Vanitha Nithianadam, et al., bioRxiv - Neuroscience 2024

  Quote: ... GFP (1:1,000, JL-8, Clontech), GABARAP/Atg8a (1:2,000 ...
• Split GFP assay to show chloroplast targeting of Agrobacterium VirD2 protein

  Aki Matsuoka, Julia L. Ferranti, Pal Maliga, bioRxiv - Bioengineering 2023

  Quote: ... Monoclonal Antibody (JL-8) (632381; Clontech) in 1:2,000 dilution ...
• Proteomic analysis identifies novel binding partners of BAP1

  Roy Baas, et al., bioRxiv - Biochemistry 2021

  Quote: ... Living Colors (GFP) (JL-8, 632380, Clontech), α-Tubulin (DM1A ...
• Deficiency in the endocytic adaptor protein PHETA1/2 impairs renal and craniofacial development

  Kristin M. Ates, et al., bioRxiv - Developmental Biology 2019

  Quote: ... A monoclonal GFP antibody (JL-8; Clontech) and a monoclonal HA antibody (sc-7392 ...
• Functional dissection of human mitotic genes using CRISPR-Cas9 tiling screens

  Jacob A. Herman, et al., bioRxiv - Cell Biology 2021

  Quote: ... anti-GFP clone JL-8 (Takara 632381) 0.5 µg/mL ...
• Optimization of genomewide CRISPR screens using AsCas12a and multi-guide arrays

  Sakina Petiwala, et al., bioRxiv - Genetics 2022

  Quote: ... 8 μL dNTPs (Clontech Takara Cat# 4030), 5μL DMSO (Sigma Aldrich Cat# D9170-5VL) ...
• Optimization of genomewide CRISPR screens using AsCas12a and multi-guide arrays

  Sakina Petiwala, et al., bioRxiv - Genetics 2022

  Quote: ... 8 μL dNTPs (Clontech Takara Cat# 4030), 5μL DMSO (Sigma Aldrich Cat# D9170-5VL) ...
• GPI-anchored Gas1 protein regulates cytosolic proteostasis in yeast

  Yuhao Wang, Linhao Ruan, Rong Li, bioRxiv - Cell Biology 2023

  Quote: ... mAb clone JL-8 (632381) from Takara Bio ...
• Lipid Biosynthesis Perturbation Impairs Endoplasmic Reticulum-Associated Degradation

  Samantha M. Turk, et al., bioRxiv - Cell Biology 2022

  Quote: ... mouse anti-GFP (Clone JL-8; Clontech) at 1:1,000 ...
• Scavenger receptor endocytosis controls apical membrane morphogenesis in the Drosophila airways

  Ana S. Pinheiro, et al., bioRxiv - Developmental Biology 2022

  Quote: ... mouse GFP (1:200, JL-8, Clontech) gp anti-Verm (Wang et al. ...
• Metabolic regulation of misfolded protein import into mitochondria

  Yuhao Wang, et al., bioRxiv - Cell Biology 2023

  Quote: ... mAb clone JL-8 (632381) from Takara Bio.
• Ablation of mpeg+ macrophages exacerbates mfrp-related hyperopia

  Zachary J. Brandt, et al., bioRxiv - Genetics 2021

  Quote: 1:500 mouse anti-GFP (Takara, JL-8)
• A Lentiviral Envelope Signal Sequence is a Tetherin Antagonizing Protein

  James H. Morrison, Eric M. Poeschla, bioRxiv - Microbiology 2021

  Quote: ... mouse anti-GFP (JL-8 clone, Takara Bio) or mouse anti-alpha-tubulin (Sigma) ...
• Digital polymerase chain reaction in an array of microfluidic printed droplets

  Yongfan Men, et al., bioRxiv - Molecular Biology 2019

  Quote: ... and 8 μL of RNase-free water (Takara). The experiment of each sample was performed more than three times on a newly prepared printing chip and PCR substrate ...
• Control of PHYTOCHROME KINASE SUBSTRATE subcellular localization and biological activity by protein S-acylation

  Ana Lopez Vazquez, et al., bioRxiv - Plant Biology 2022

  Quote: ... anti-GFP monoclonal antibody JL-8 (632381, Clontech) was used at 1/3000 ...
• Activity-dependent stabilization of nascent dendritic spines requires non-enzymatic CaMKIIα function

  Nicole Claiborne, Karen Zito, bioRxiv - Neuroscience 2022

  Quote: ... except 6-8 µg of DsRed-Express (Clontech) and 6 μg of mEGFP-tagged constructs or 5-10 μg of mEGFP were coated onto 6-7 mg of 1.6 μm gold beads ...
• Decreasing Mitochondrial RNA Polymerase Activity Reverses Biased Inheritance of Hypersuppressive mtDNA

  Daniel Corbi, Angelika Amon, bioRxiv - Cell Biology 2021

  Quote: ... Mouse Anti-GFP (1:1000; JL-8, Clontech). Blots were washed three times with PBST and probed with secondary antibodies diluted in PBS with 1% milk and 1% BSA for one hour at 4°C ...
• Neurexin 3 is required for the specific S-cone to S-cone bipolar cell synapse in the mammalian retina

  Vincent P Kunze, et al., bioRxiv - Neuroscience 2023

  Quote: ... and transferred into 8 µL lysis buffer (Clontech SMARTer Ultra Low Input RNA Kit for Sequencing - v3) ...
• Cell cycle-dependent organization of a bacterial centromere through multi-layered regulation of the ParABS system

  Jovana Kaljević, Tung B. K. Le, Géraldine Laloux, bioRxiv - Microbiology 2023

  Quote: ... GFP tag (JL-8 monoclonal antibody from Takara), and mCherry (polyclonal antibody from Thermo Fisher ...
• Complete depletion of Arabidopsis linker histones impairs the correlations among chromatin compartmentalization, DNA methylation and gene expression

  Zhenfei Sun, et al., bioRxiv - Plant Biology 2021

  Quote: ... 4 U and 8 U of MNase (TaKaRa 2910A) in MNase digestion buffer containing 20 mM Tris–HCl (pH 8.0) ...
• Disrupting the ciliary gradient of active Arl3 affects rod photoreceptor nuclear migration

  Amanda M. Travis, et al., bioRxiv - Cell Biology 2022

  Quote: ... monoclonal anti-GFP JL-8 (1:1000, Clontech 632380), polyclonal rabbit anti-RP2 (1:1000 ...
• Manganese is a Physiologically Relevant TORC1 Activator in Yeast and Mammals

  Raffaele Nicastro, et al., bioRxiv - Cell Biology 2022

  Quote: Primary antibodies against GFP (Clontech, JL-8; 1:5000), G-6-PDH (Sigma-Aldrich ...
• Wnt Regulation: Exploring Axin-Disheveled interactions and defining mechanisms by which the SCF E3 ubiquitin ligase is recruited to the destruction complex

  Kristina N. Schaefer, et al., bioRxiv - Cell Biology 2019

  Quote: ... Primary Antibodies: anti-GFP (JL-8; Clontech, 1:1000), anti-Flag (Sigma-Aldrich ...
• Vpr Co-assembles with Gag during HIV-1 Assembly

  Kate Bredbenner, Sanford M Simon, bioRxiv - Microbiology 2020

  Quote: ... a monoclonal anti-GFP (Living Colors JL-8, Clontech), or a monoclonal anti-β-actin (Sigma-Aldrich ...
• Meiotic resetting of the cellular Sod1 pool is driven by protein aggregation, degradation, and transient LUTI-mediated repression

  Helen M. Vander Wende, et al., bioRxiv - Cell Biology 2022

  Quote: ... mouse α-GFP JL-8 (cat. no. 632381, Takara) used at a concentration of 1:2,000 ...
• Conditional knockout of Shank3 in the ventral CA1 by quantitative in vivo genome-editing impairs social memory

  Myung Chung, et al., bioRxiv - Neuroscience 2024

  Quote: ... Protamine sulfate (Final concentration: 8 μg/ml, Takara Bio) was added ...
• Regulation of Traction Force through the Direct Binding of Basigin and Calpain 4

  Bingqing Hao, Karen A. Beningo, bioRxiv - Cell Biology 2023

  Quote: ... 30 cycles of 98 °C for 10 sec followed by 68 °C for 1 min using PrimeSTAR HS DNA Polymerase with GC buffer (Takara, R044A). The primers used for the purpose of inserting Capn4 into pCWX200 and pLexA were ...
• Traction Force and Mechanosensing can be Functionally Distinguished Through the Use of Specific Domains of the Calpain Small Subunit

  Bingqing Hao, Karen A. Beningo, bioRxiv - Cell Biology 2023

  Quote: ... 30 cycles of 98 °C for 10 sec followed by 68 °C for 1 min using PrimeSTAR HS DNA Polymerase with GC buffer (Takara, R044A). The primers used were as follows ...
• Distinct gene expression dynamics in germ line and somatic tissue during ovariole morphogenesis in Drosophila melanogaster

  Shreeharsha Tarikere, et al., bioRxiv - Developmental Biology 2021

  Quote: ... mRNA was isolated using PrepX PolyA-8 protocol (Takara 640098). The mRNA samples were then processed for cDNA preparation using PrepX mRNA-8 (Takara 640096 ...
• Agewise mapping of genomic oxidative DNA modification demonstrates oxidative-driven reprogramming of pro-longevity genes

  Lynn Htet Htet Aung, et al., bioRxiv - Genetics 2020

  Quote: Custom oligonucleotides containing 8-OHdG bases were purchased from Takara Bio Inc ...
• Dynein light chain-dependent dimerization of Egalitarian is essential for maintaining oocyte fate in Drosophila

  Hannah Neiswender, et al., bioRxiv - Cell Biology 2021

  Quote: ... mouse anti-GFP (Clontech, JL-8, 1:5000 for western), rabbit anti-GFP (Chromotek ...
• In vivo proximity biotin ligation identifies the interactome of Egalitarian, a Dynein cargo adaptor

  Frederick C. Baker, et al., bioRxiv - Cell Biology 2021

  Quote: ... mouse anti-GFP (Clontech, JL-8, 1:5000 for western), mouse anti-Dhc (Developmental Studies Hybridoma Bank ...
• UBE3A and transsynaptic complex NRXN1-CBLN1-GluD1 in a hypothalamic VMHvl-arcuate feedback circuit regulates aggression

  Yi Nong, et al., bioRxiv - Neuroscience 2023

  Quote: ... Green Fluorescent Protein (GFP) antibody (JL-8, 1:500, Clontech), Red Fluorescent Protein Antibody (DsRed ...
• Novel proteostasis reporter mouse reveals different effects of cytoplasmic and nuclear aggregates on protein quality control in neurons

  Sonja Blumenstock, et al., bioRxiv - Neuroscience 2020

  Quote: ... The following primary antibodies were used: GFP (JL-8, Clontech, #632381), Tubulin (Covance ...
• Selective Microautophagy of Proteasomes is Initiated by ESCRT-0 and Is Promoted by Proteasome Ubiquitylation

  Jianhui Li, Mark Hochstrasser, bioRxiv - Cell Biology 2021

  Quote: ... JL-8 anti-GFP monoclonal antibody (TaKaRa, catalog # 632381, lot # A8034133) at 1:2,000 dilution ...
• A constitutively monomeric UVR8 photoreceptor allele confers enhanced UV-B photomorphogenesis

  Roman Podolec, et al., bioRxiv - Plant Biology 2020

  Quote: ... anti-GFP (Living Colors® A.v. Monoclonal Antibody, JL-8; Clontech), anti-CHS (sc-12620 ...
• Functional interdependence of the actin nucleator Cobl and Cobl-like in dendritic arbor development

  Maryam Izadi, et al., bioRxiv - Cell Biology 2021

  Quote: ... Monoclonal mouse anti-GFP antibodies (JL-8) were from Clontech (632381). Monoclonal mouse anti-Flag (M2 ...
• Multifactorial chromatin regulatory landscapes at single cell resolution

  Michael P. Meers, et al., bioRxiv - Genomics 2022

  Quote: ... 16 µL 100x DAPI and 8 µL ICELL8 Second Diluent (Takara) were added and incubated 10 minutes at RT ...
• P38 kinases mediate NLRP1 inflammasome activation after ribotoxic stress response and virus infection

  Lea-Marie Jenster, et al., bioRxiv - Immunology 2022

  Quote: ... mouse anti-GFP clone JL-8 (Takara Bio Cat# 632380, RRID:AB_10013427), rabbit anti-FLAG clone D6W5B (Cell Signaling Technology Cat# 14793 ...
• BBX proteins promote HY5-mediated UVR8 signaling in Arabidopsis

  Roman Podolec, et al., bioRxiv - Plant Biology 2021

  Quote: ... anti-GFP (Living Colors® A.v. Monoclonal Antibody, JL-8; Clontech), anti-HY5 (Oravecz et al. ...
• Instantaneous visual genotyping and facile site-specific transgenesis via CRISPR-Cas9 and phiC31 integrase

  Junyan Ma, et al., bioRxiv - Bioengineering 2022

  Quote: ... EGFP was detected using the JL-8 mouse monoclonal antibody (Clontech). For IHC ...
• Multi-omics analyses of MEN1 missense mutations identify disruption of menin-MLL and menin-JunD interactions as critical requirements for molecular pathogenicity

  Koen M.A. Dreijerink, et al., bioRxiv - Molecular Biology 2022

  Quote: ... GFP expression was verified using immunoblotting using GFP (JL-8-Clontech) and α-tubulin (CP06 Calbiochem ...
• Atg15 is a vacuolar phospholipase that disintegrates organelle membranes

  Yasunori Watanabe, et al., bioRxiv - Molecular Biology 2023

  Quote: ... Membranes were then incubated with anti-GFP antibody (JL-8; Clontech) at a dilution of 1:5,000 ...