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Citations for Takara Bio :
201 - 250 of 695 citations for 7 9 Diazaspiro 4.5 decane 6 8 10 trione 7 ethyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: HEK293T/17 cells were co-transfected with 0.8 pmol SINEUP-GFP or miniSINEUP-GFP FRAM (0.8 pmol) and 0.2 pmol sense pEGFP-C2 plasmids (Clontech) in each well of a 6-well culture plate ...
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bioRxiv - Bioengineering 2024Quote: ... GFP was detected with a monoclonal mouse anti-GFP antibody (JL-8, Takara Bio USA, San Jose, CA) in Western blot analyses.
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bioRxiv - Molecular Biology 2021Quote: ... with 10% FBS (TaKaRa, 631106). HCT116 OsTIR1 cells (kindly provided by Masato Kanemaki ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with 10% FBS (Clontech), 100 units/mL penicillin ...
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bioRxiv - Cell Biology 2019Quote: ... supplemented with 10 % FBS (Clontech), penicillin ...
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bioRxiv - Molecular Biology 2019Quote: ... 10 μL PCR mixture (TaKaRa), 1 μL upstream primer (10 μmol/L) ...
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bioRxiv - Molecular Biology 2019Quote: ... supplemented with 10% FBS (Clontech) in a humidified atmosphere of 5% CO2 at 37°C.
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bioRxiv - Systems Biology 2021Quote: ... supplemented with 10% FBS (Clontech), GlutaMAX supplement (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2022Quote: ... supplemented with 10% FBS (Clontech), 100 units/mL penicillin ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 10% FBS (Clontech) and 2 mM L-glutamine (PAN Biotech ...
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bioRxiv - Genomics 2023Quote: ... supplemented with 10% FBS (Takara), 1% NEAA (Gibco) ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were seeded in one well of a 6 well plate in supplemented DEF-CS (Takara). After a recovery period of 5 days ...
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bioRxiv - Genomics 2019Quote: ... and the expression of IFN-β and IL-6 were measured by quantitative PCR (TAKARA, RR820A). The sequences of the primers were listed in the Supplementary Table 4.
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bioRxiv - Synthetic Biology 2020Quote: ... Concentrated virus was plated on non-TC treated 6-well plates coated with retronectin (Takara T100B), and spun for 90 minutes at 1200xg ...
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bioRxiv - Plant Biology 2019Quote: ... proteins were transferred to PVDF membrane and blotted proteins were incubated in a 1:10,000 dilution of mouse monoclonal anti-GFP antibody (Living Colors JL-8, Clontech) followed by 1:5000 horseradish peroxidase conjugated sheep anti-mouse IgG antibody (Amersham ...
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bioRxiv - Neuroscience 2021Quote: ... RNA samples with RIN values >8 were used for library preparation according to manufacturer’s instructions (SmartSeqv4 RNASeq kit, Clontech) or manual library preparation for Ampliseq analysis (Ion AmpliSeq Lib Kit Plus) ...
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bioRxiv - Microbiology 2021Quote: ... H6-Gfp-FtsZ and FtsZ were mixed in a 1:1 ratio for a final concentration of 8 µM in assembly buffer with GMPCPP (0.2 mM) and resuspended Talon Cobalt beads (Takara) and added ...
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bioRxiv - Cell Biology 2020Quote: ... To detect the transgenic GFP-tagged Abl proteins we used anti-GFP (JL-8, 1:500 or 1:1000, Clontech). Anti-αTubulin (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA was pre-amplified by adding 2 uL of cDNA from each sample to 8 uL of preamp master mix [5 uL TaKaRa premix Taq polymerase (Clontech), 2.5 uL 0.2X Taqman pooled probe ...
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bioRxiv - Molecular Biology 2019Quote: ... Same volume of samples were loaded on 4-16% gradient SDS-PAGE gels and analyzed by western blot analysis using EGFP (JL-8; Clontech), penta-HIS (QIAGEN ...
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bioRxiv - Cell Biology 2021Quote: ... cells were stably transfected with a plasmid encoding human full-length keratin 8 with an EYFP tag at its carboxyterminus (Windoffer et al., 2004; recloned into pEYFP-N1 (Clontech) with BamHI and EcoRI) ...
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bioRxiv - Plant Biology 2021Quote: ... Membranes were blocked with 5% milk overnight at 4°C or 1h at room temperature for Anti-GFP JL-8 (1:4000; Clontech, California ...
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bioRxiv - Microbiology 2020Quote: ... Proteins induced by 1 mM IPTG entered inclusion bodies and were extracted with 8 M urea and purified with TALON Metal Affinity Resin (Takara) under denaturing conditions ...
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bioRxiv - Developmental Biology 2019Quote: ... 10 ng total RNA was reverse transcribed and full-length cDNA was specifically amplified by 8 PCR cycles using SMART-Seq v4 Ultra Low Input RNA Kit (Clontech) (31) ...
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bioRxiv - Immunology 2022Quote: ... or the expression vector LentiCRISPRv2 (coding for guideRNA) in a 3:1:3 ratio for 8 hours using calcium phosphate transfection (CalPhos Mammalian Transfection Kit, Takara Bio Europe ...
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bioRxiv - Cell Biology 2022Quote: ... The samples were subjected to 4-20% SDS-PAGE and analyzed by immunoblotting with the following primary antibodies: anti-GFP mouse monoclonal antibody (JL-8, Takara), GFP rabbit polyclonal antibody (PABG1 ...
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bioRxiv - Biophysics 2022Quote: ... The stability of the fusion constructs was verified by gel electrophoresis and immunoblotting using an anti-GFP primary antibody (JL-8 monoclonal, Takara). Point mutations were introduced by site-directed mutagenesis (New England Biosciences) ...
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bioRxiv - Bioengineering 2023Quote: ... Cerulean and Myo7b blots were stained for 1 h at room temperature (RT) or overnight at 4 °C using the mouse anti-GFP (detects cerulean, 1:2000, JL-8, Clontech, Takara) and the mouse anti-β-tubulin antibody (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... and AAV1-syn-F14F15S-sTpEptTA_v2 were made by transfecting 8×15c plates (per virus) of HEK 293T cells using Xfect transfection reagent (Takara 631318). Briefly ...
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bioRxiv - Plant Biology 2023Quote: ... Recombinant proteins corresponding to the full-neck regions of myosin VIIIs were solubilized using 8 M urea and purified using a Ni-NTA His60 column (Takara), or for GST-tandem-IQ fusions ...
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bioRxiv - Microbiology 2023Quote: ... Blots were probed for 1h at room temperature (RT) with a mouse anti-GFP antibody (Living Colors -JL-8, BD Biosciences Clontech) in TBS-T buffer (20 mM Tris ...
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bioRxiv - Molecular Biology 2022Quote: ... using the primers described previously(6) and cloned into pmCherry-C1 (Takara Bio Inc., San Jose, CA). To silence HNRNPK expression ...
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bioRxiv - Genetics 2022Quote: ... then combined with 6 µL cDNA and 15 µL Mighty Mix T4 DNA ligase reaction mix (Takara) and incubated overnight at 16 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... Aliquots of total RNA (1 μg) were reverse transcribed using pd(N)6 random primer (Takara Bio) and Moloney murine leukemia virus (M-MLV ...
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bioRxiv - Microbiology 2020Quote: ... Cellular RNA was isolated at 6 hpi using the CellAmp Direct RNA Prep Kit (3732; Takara, Japan). The RNA was then diluted in water and boiled ...
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bioRxiv - Genomics 2020Quote: ... 10 μL of Mixture (TaKaRa, Japan), 1 μL upstream primers (10 μmol/L ...
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bioRxiv - Microbiology 2022Quote: ... 10 μL of Clone AMP (Takara), and 300 nM each of primers ks1 and mf83 ...
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bioRxiv - Developmental Biology 2019Quote: ... 10 U μl–1 SMARTScribe (Clontech) and 10 U μl–1 RNASin plus (Promega) ...
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Differential turnover of Nup188 controls its levels at centrosomes and role in centriole duplicationbioRxiv - Cell Biology 2019Quote: ... 10% Tet system approved FBS (Takara), 100 units/ml penicillin and 100 μg/ml streptomycin (Thermo Fisher Scientific) ...
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bioRxiv - Synthetic Biology 2021Quote: ... and 10% Fetal Bovine Serum (Clontech). Cells were grown at 37°C with 5% CO2 and passaged using 0.05% Trypsin-EDTA solution (Thermo Fisher ...
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bioRxiv - Synthetic Biology 2020Quote: ... 10% Tet Approved FBS (Clontech Laboratories), and 1X Penicillin/Streptomycin (Gibco) ...
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bioRxiv - Neuroscience 2019Quote: ... 10-15 µg of EGFP (Clontech) was coated onto 6-8 mg of 1.6 µm gold beads.
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bioRxiv - Plant Biology 2022Quote: ... using 10 pmol random nonamers (Takara) in 20 μl reaction volume and according to manufacturers’ instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... with 10% FBS (Takara Bio, 631368) and 1x penicillin-streptomycin in 24-well glass-bottomed No ...
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bioRxiv - Molecular Biology 2024Quote: ... and 10 U recombinant inhibitor (TaKaRa) for samples containing biological inhibitor ...
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bioRxiv - Synthetic Biology 2024Quote: ... supplemented with 10% FBS (Takara 632180). LentiX cells (Takara 632180 ...
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bioRxiv - Molecular Biology 2021Quote: ... DMEM supplemented with 10% fetal bovine serum (FBS) and 10 μl DNase I (Takara Bio, Shiga, Japan) was then added to the samples and incubated for 5 min at room temperature (RT) ...
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bioRxiv - Developmental Biology 2021Quote: ... cDNA was amplified using 8 µl of the PCR MasterMix (SMART-Seq v5 Ultra Low Input RNA Kit for Sequencing, Takara Bio) with 25 cycles of amplification ...
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bioRxiv - Neuroscience 2020Quote: ... single cells were sorted into individual wells of 8-well PCR strips containing lysis buffer from the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara 634894) with RNase inhibitor (0.17 U/μl) ...
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bioRxiv - Neuroscience 2020Quote: ... single cells were sorted into individual wells of 8-well PCR strips containing lysis buffer from the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara 634894) with RNase inhibitor (0.17 U/μl) ...