Labshake search
Citations for Takara Bio :
4851 - 4900 of 5541 citations for Galactose Colorimetric Detection Kit 2 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2022Quote: All plasmid fusions assembled in-house were generated through standard ligation-based methods using the In-Fusion cloning kit according to manufacturer protocols (Takara, 638920). MBNL1 RNA-interaction and chelation mutant sequences were designed based on plasmids generated by Andrew Berglund (Purcell et al. ...
-
bioRxiv - Cell Biology 2022Quote: ... Donor vectors containing the GFP or mKate2 coding sequence flanked by 1kb homology arms adopted from both 3′ and 5′ sides of the insertion site were generated by cloning of PCR-amplified tags and arms into linearized pGEM-3z vector by In-Fusion HD Cloning Kit (Takara Bio). Obtained gRNA expression plasmids and donor plasmids were injected into the y w embryos with a final concentration of 120 ng/ul for each ...
-
bioRxiv - Biophysics 2022Quote: Site-directed mutagenesis to construct the variants was performed using the PrimeSTAR mutagenesis basal kit (Takara Bio Inc., Kusatsu, Shiga, Japan) with the pPICZαA vector containing the mature Forham1 gene as the template ...
-
bioRxiv - Cell Biology 2022Quote: Total RNA extraction and reverse transcription of RNA into cDNA were performed based on the method described as manufacturer’s protocol of Prime Script TMΠ 1stStrand cDNA Synthesis Kit andTB GreenTM Premix Ex TaqTM II (TaKaRa, Beijing, China). qRT-PCR was performed with ChamQ Universal SYBR qPCR Master Mix (Vazyme ...
-
bioRxiv - Cell Biology 2022Quote: ... NM_001123007) was amplified from 24 hpf embryo RNA using the PrimeScript II High Fidelity One Step RT-PCR kit (Takara, R026A) and the following primers ...
-
bioRxiv - Cancer Biology 2022Quote: Sequencing libraries were prepared from total RNA using the Smarter Stranded Total RNA-seq Kit v2 Pico Input Mammalian (Takara, Japan), followed by size distribution analysis in the Agilent 2100 Bioanalyzer using the High Sensitivity DNA Kit (Agilent) ...
-
bioRxiv - Immunology 2022Quote: Illumina Compatible low input mRNA libraries were prepared using the Smart-Seq V4 Ultra Low Input RNA kit (Takara Bio, USA) and KAPA HyperPlus Library Preparation kit (Roche) ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... The low-yield gwj02 sample was prepared with the ThruPLEX DNA-Seq Kit (Takara Bio USA, Inc., Mountain View, CA, USA). Sequences (2×250 bp ...
-
bioRxiv - Plant Biology 2022Quote: ... purified with Fast pure gel DNA extraction mini kit (DC301, Nanjing Vazyme Biotech, China) and then cloned into the pMD19-T vector (TaKaRa, Japan). The CRISPR-edited sequences were screened by Sanger sequencing of pMD19-T vector.(K ...
-
bioRxiv - Plant Biology 2022Quote: ... 1 μg of total RNA was reverse transcribed into cDNA using PrimeScript™ II 1st Strand cDNA Synthesis Kit (TaKaRa, Japan). qRT-PCR was performed with Roche LightCycler 480 Real Time PCR system (Roche ...
-
bioRxiv - Molecular Biology 2022Quote: ... The full-length TLN1 was inserted into a pEGFP-N2 vector using the In-Fusion HD cloning kit (Takara Bio USA). Silent mutations on TLN1 were generated with the QuikChange site-directed mutagenesis kit (Agilent Technologies ...
-
bioRxiv - Immunology 2022Quote: ... selected RNAs from cDCs were used to amplify the cDNA using the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech-Takara). Amplified (1 ng ...
-
bioRxiv - Immunology 2022Quote: ... Libraries for mRNA sequencing were prepared starting from 10 ng tot RNA for each sample by using the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech-Takara). All samples were sequenced on an Illumina NextSeq 550 at an average of 17,5 million 75-bp single-end reads.
-
bioRxiv - Microbiology 2022Quote: ... cDNA was synthesized using the PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time) (TaKaRa Bio Inc., Dalian, China) from 1 μg total RNA in a final volume of 20 μL according to the provided protocol ...
-
bioRxiv - Microbiology 2022Quote: ... and the plasmid DNAs were isolated using the NucleoBond® Xtra Midi EF plasmid mid-prep kit (Takara Bio USA Inc.) according to the manufacturer’s instructions.
-
bioRxiv - Microbiology 2022Quote: Total RNA was extracted from HT-29 cells using the CellAmp Direct RNA Prep Kit for RT-PCR (3732; TaKaRa, Japan) after the introduction of genomic DNA or Poly (I ...
-
bioRxiv - Plant Biology 2022Quote: ... Genomic DNA removal and first-strand cDNA synthesis were carried out using a TAKARA first-strand cDNA synthesis kit (TaKaRa, Japan). Quantitative real-time PCR (qRT-PCR ...
-
bioRxiv - Plant Biology 2022Quote: ... The genomic fragments were assembled and subcloned into a modified version of pBluescript II KS (StrataGen, pBlueMLAPUCAP) using the InFusion® HD Cloning Plus kit (Clontech), yielding the construct pBS-TPS02r and pBS-TPS02g ...
-
bioRxiv - Neuroscience 2022Quote: ... prepared freshly with 940 nL of Lysis Buffer 1 (1 μL of 10X reaction buffer is diluted in 2.75 μL of water) (SMART-Seq v4 Ultra Low input RNA kit; Takara Bio Inc.) per well ...
-
bioRxiv - Plant Biology 2022Quote: ... The expression analysis of each target gene was performed quantitatively by real-time RT-PCR method after cDNA synthesis using PrimeScript™ RT reagent Kit with gDNA Eraser (TaKaRa) and TB Green® Premix Ex Taq™ II (Tli RNaseH Plus ...
-
bioRxiv - Plant Biology 2022Quote: ... and a Gateway® cassette amplified from vector pGWB14 (kindly provided by Pinghua Li, Shandong Agricultural University, Shandong, China) was ligated using the In-Fusion kit (Clontech, USA) for generation the binary destination vector pCSpeGH (Sequence deposited in NCBI Gene Bank with the accession number MW186668) ...
-
bioRxiv - Physiology 2023Quote: ... The adenovirus titer in plaque-forming units (PFU) was determined using a Adeno-X Rapid Titer kit (Clontech, Palo Alto, CA).
-
bioRxiv - Plant Biology 2023Quote: Total RNA was extracted from plant tissue at the site of inoculation using a MiniBEST Plant RNA Extraction Kit (TaKaRa, China) as per kit instructions ...
-
bioRxiv - Immunology 2022Quote: ... RNA quality was assessed using an Agilent Fragment Analyzer and ten nanograms of total RNA was used as input for cDNA synthesis using the Clontech SMART-Seq v4 Ultra Low Input RNA kit (Takara Bio) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2022Quote: ... total RNA was cleaned by genomic removal and then reversely transcribed (Prime Script RT reagent Kit with gDNA Eraser, Takara, China). The rRNA removal ...
-
bioRxiv - Microbiology 2022Quote: ... the viral RNA was isolated and amplified by RT-PCR using the PrimeScript II High Fidelity One Step RT-PCR Kit (Takara Bio) and specific primers ...
-
bioRxiv - Molecular Biology 2022Quote: The G3BP1 cDNA sequence was amplified from a HeLa-JVM total cDNA library and concurrently inserted in-frame with a mCherry-coding sequence into a lentiviral vector (pCW)110 using the in-Fusion Cloning Kit (TaKaRa Bio). The HERC5 ...
-
bioRxiv - Molecular Biology 2022Quote: Synthesis of cDNA with biotinylated 3’ adaptor ligation was performed using a Small RNA Cloning Kit (Takara Bio Inc., Kusatsu, Japan) as previously reported (20 ...
-
bioRxiv - Immunology 2022Quote: ... Ten ng total RNA was used as template for SMARTer® Stranded Total RNA-Seq Kit – Pico Input Mammalian (Takara Bio.) following the manufactureŕs recommendation.
-
bioRxiv - Genetics 2022Quote: ... An aliquot of 1 μg of the total RNA was used to synthesize cDNA using PrimeScript™ RT reagent Kit with gDNA eraser (Takara). qRT-PCR analysis was performed on a StepOnePlus Real-Time PCR system (Applied Biosystems ...
-
bioRxiv - Molecular Biology 2022Quote: ... pcDNA4TO-bio-myc-K294A was prepared from pcDNA4TO-bio-myc-mCE by using In-Fusion Site Directed Mutagenesis kit (Takara Bio). The HA-NCK1-M3 construct was a generous gift from Dr ...
-
bioRxiv - Synthetic Biology 2024Quote: ... 23S rRNA mutations were introduced to the pLK35 plasmid using the corresponding primer set (Supplementary Table 5) and the In-Fusion Cloning kit (Takara Bio). All sequences were confirmed with full plasmid nanopore sequencing (Plasmidsaurus and Elim Bio).
-
bioRxiv - Immunology 2024Quote: ... A total of 1 μg of total RNA was reverse-transcribed with random primers using the PrimeScript™ 1st strand cDNA Synthesis Kit (TaKaRa) according to the manufacturer’s protocols ...
-
bioRxiv - Zoology 2024Quote: ... RNA was extracted using Isogen-LS (Nippon Gene) and RT-qPCR was performed using the One-Step PrimeScript RT-PCR Kit (Takara Bio) on a 7500 Real-Time RT-PCR System (Applied Biosystems) ...
-
bioRxiv - Plant Biology 2024Quote: ... the 5 μl samples were used as input into the SMART-Seq v4 Ultra Low Input RNA Kit (Takara Bio USA) (for parasitic J3 library generation ...
-
bioRxiv - Immunology 2023Quote: ... The expression vectors for the S variants with multiple amino acid changes or deletions were generated using the In-Fusion HD cloning kit (Takara Bio). The pcDNA3.1-hACE2 used to express human ACE2 (hACE2 ...
-
bioRxiv - Cell Biology 2024Quote: ... the coding sequence of each gene was amplified from whole larva cDNA using PrimeScript RT-PCR Kit (Takara, cat # RR014-A). The amplified sequence was then purified through gel extraction (Magen HiPure Gel Pure DNA Mini kit ...
-
bioRxiv - Molecular Biology 2024Quote: ... PCR products were analysed by gel electrophoresis and positive products were purified using the NucleoSpin Gel and PCR clean up kit (Clontech, Takara Bio) before being sent for sequencing by Eurofins Genomics (Germany) ...
-
bioRxiv - Developmental Biology 2023Quote: ... Reverse transcription of 500 ng purified total RNA was performed by PrimeScript RT reagent kit with gDNA Eraser (Takara, Dalian, China). RT-qPCR was performed with SYBR green master mix (Takara ...
-
bioRxiv - Neuroscience 2024Quote: The annealed oligos were ligated with the pGuide-it-sgRNA1 Vector and prepared using the FastGene Plasmid Mini Kit (Nippon Genetics) and NucleoBond Xtra Midi (Takara Bio). Transfection into HEK-293T cells was performed according to the manufacturer’s instruction ...
-
bioRxiv - Neuroscience 2024Quote: Total RNA (500 ng) was reverse transcribed to cDNA using the PrimeScript™ RT Reagent Kit with gDNA Eraser (Takara, RR047A). qPCR was carried out using PowerUp™ SYBR™ Green Master Mix kit and detected by QuantStudio3 system (Thermo Fisher) ...
-
bioRxiv - Neuroscience 2024Quote: ... The cDNA templates were synthesized from the purified RNA using the PrimeScript 1st strand cDNA Synthesis Kit (Takara Bio, Kusatsu, Japan) with oligo (dT)20 primers ...
-
bioRxiv - Microbiology 2024Quote: ... DNase-treated total RNA was used to prepare cDNA libraries with the SMARTer smRNA-Seq Kit (Takara Bio, Mountain View, CA). Libraries were sequenced as 50 bp single-end reads on an Illumina HiSeq sequencer ...
-
bioRxiv - Molecular Biology 2024Quote: ... 500 ng of total RNA underwent reverse transcription to generate cDNA using the primeScriptTM RT Master Mix reagent kit (TaKaRa, Japan). Quantitative assessment of relative RNA expression was performed via RT-qPCR using SYBR Premix Ex TaqTM (TaKaRa ...
-
bioRxiv - Molecular Biology 2024Quote: 900 ng of total RNA isolated from HUVEC was used as input for SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian (Takara Bio). Sequencing was performed on the NextSeq500 instrument (Illumina ...
-
bioRxiv - Microbiology 2024Quote: ... for genotype analysis was extracted using the Qiagen DNeasy Blood and Tissue kit and PCR analysis was done with CloneAmp™ HiFi PCR Premix (Takara) or Phusion polymerase (NEB).
-
bioRxiv - Neuroscience 2024Quote: ... RNA was extracted as described for RNA sequencing and cDNA was generated using the PrimeScript RT reagent kit (Takara, cat. #RR037A) using a mix of oligo dT and random hexamer primers ...
-
bioRxiv - Cell Biology 2024Quote: ... Complementary DNA (cDNA) synthesis was carried out with 1 μg of RNA using the PrimeScript RT reagent kit (Takara-#RR037A-4). The cDNA was diluted and semiquantitative and Real-time PCR techniques were performed ...
-
bioRxiv - Neuroscience 2023Quote: ... expressing UAS-eGFP was subjected to 14 cycles of PCR amplification (SMARTer Seq V4 Ultra Low Input RNA Kit; Takara Bio). 1 ng of amplified RNA was used to prepare cDNA libraries (Nextera XT DNA library preparation kit ...
-
bioRxiv - Developmental Biology 2024Quote: ... RNA samples were purified using a SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara, Mountain View, CA, USA), followed by library preparation using the Nextera XT DNA Library Prep kit (Illumina ...