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Citations for Takara Bio :
4801 - 4850 of 5041 citations for QuantiChrom Arginase Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... One hundred microliter cell culture supernatant was harvested for viral RNA extraction using the MiniBEST Viral RNA/DNA Extraction Kit (Takara, Cat no. 9766) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: Transcription start sites were characterised by cloning and sequencing 5’ RACE products using the SMARTer RACE 5’/3’ kit (Takara Bio USA, Inc). In summary ...
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bioRxiv - Bioengineering 2022Quote: ... Reverse transcription to obtain cDNA was performed according to the instructions of the PrimeScript 1st Strand cDNA Synthesis Kit (Takara Biomedical Technology (Beijing) Co. ...
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bioRxiv - Microbiology 2022Quote: ... The PCR product was ligated into the NotI site of the pNEN142 vector (Minetoki et al., 2003) with an In-Fusion HD Cloning Kit (Takara Bio, Kusatsu, Japan); this vector contains the improved promoter of the A ...
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bioRxiv - Neuroscience 2022Quote: ... mRNA 500 ng was used for cDNA synthesis in 10 μl volume according to the manufacturer’s instructions of PrimeScriptTM RT-PCR kit (Takara Bio Inc., Shiga, Japan). Real-time RT-PCR was then performed using the KAPA SYBR FAST qPCR Master Mix (2x ...
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bioRxiv - Cell Biology 2022Quote: ... and Foxa3 were obtained by reverse transcription PCR using a template RNA extracted from a C57BL/6J liver using a PrimeScript™ II 1st strand cDNA Synthesis Kit (TAKARA Bio, Japan). The coding sequences of the primers used are listed in Table S3 ...
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bioRxiv - Developmental Biology 2019Quote: ... First-strand cDNA (from total RNA) was synthesized according to the SMART-Seq™ v4 Ultra™ Low Input RNA Kit protocol (Clontech Laboratories). The PCR-amplified cDNA was purified using SPRI beads (Beckmann Coulter).
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bioRxiv - Molecular Biology 2019Quote: ... A SMART-Seq v4 Ultra Low Input Kit for Sequencing was used for full-length cDNA synthesis and amplification (Clontech, Mountain View, CA), and Illumina Nextera XT library was used for sequencing library preparation ...
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bioRxiv - Physiology 2020Quote: ... containing 1 μL of 10× reaction buffer (SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing, Cat. No. 634888, Takara Bio, Kusatsu, Japan). Nuclease-free water was added to 11.5 μL ...
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bioRxiv - Cell Biology 2020Quote: ... and subsequently used as a template to generate all other HaloTag constructs using the In-Fusion HD Cloning Kit (Takara Bio USA, Inc), with the exception of pReciever-M49(HaloTag-SOX17 ...
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bioRxiv - Cancer Biology 2019Quote: ... 4 ng of RNA were used to prepare RNA libraries using the SMARTer Stranded Total RNA-seq Kit – Pico Input Mammalian (Takara Bio USA Inc.). Briefly ...
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bioRxiv - Genetics 2019Quote: ... RNA was reverse transcribed using SMART-Seq Reverse Transcriptase followed by 11 cycles of PCR amplification (SMART-Seq v4 kit, Takara Bioscience, Cat. 634890). Purification and size selection of DNA was carried out using Ampure XP beads (Beckman Coulter ...
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bioRxiv - Developmental Biology 2020Quote: ... followed by RNA isolation according to the manufacturer’s instructions (SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing, Takara Bio USA) (Figure 2A ...
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bioRxiv - Molecular Biology 2021Quote: ... One µg of total RNAs was subjected to reverse transcription into cDNAs using PrimeScriptTM RT reagent Kit with gDNA Eraser (Takara, cat. no. RR047A) according to manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: ... four sequencing libraries (one for each tissue type) were constructed using the SMARTer PCR cDNA Synthesis Kit (ClonTech, Takara Bio Inc., Shiga, Japan) by tissue type with no size selection by NovoGene ...
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bioRxiv - Plant Biology 2020Quote: ... four sequencing libraries (one for each tissue type) were constructed using the SMARTer PCR cDNA Synthesis Kit (ClonTech, Takara Bio Inc., Shiga, Japan) by tissue type with no size selection by NovoGene ...
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bioRxiv - Microbiology 2021Quote: RsmY and RsmZ expression quantification was done by RT-PCR using Mir-X miRNA qRT-PCR TB Green Kit (Takara Bio USA, Inc). For each reaction ...
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bioRxiv - Plant Biology 2021Quote: Bait constructs for Y1H analysis were produced by inserting promoter regions of SDI genes into the multiple cloning site of pTUY1H using In-Fusion® HD cloning kit (Takara Bio Inc.) and then used to transform yeast strain Y187 ...
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bioRxiv - Developmental Biology 2021Quote: ... and the total RNA was reverse transcribed into cDNA using the PrimeScript RT Reagent Kit with gDNA Eraser (TaKaRa, RR047A, Kusatsu, Shiga, Japan). The diluted cDNA was used as a template for qPCR ...
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bioRxiv - Microbiology 2019Quote: ... Japan) and cloned into a pEGFP-N1 vector that encodes EGFP [49] using an In-Fusion HD Cloning Kit (TaKaRa, Mountain View, CA). The mouse ...
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bioRxiv - Cancer Biology 2019Quote: ... First-strand cDNA was synthesized from 1 μg of total RNA using the PrimeScript™ RT reagent Kit with gDNA Eraser (TaKaRa, Dalian, China). SYBR Premix Ex Taq II (TaKaRa ...
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bioRxiv - Microbiology 2021Quote: ... The extracellular SARS-CoV-2 RNA in the culture supernatant was analyzed using SARS-CoV-2 direct detection RT-qPCR kit (RC300A; TaKaRa-Bio, Shiga, Japan). The infectivity of SARS-CoV-2 in the culture supernatants was determined at 48 h post-infection ...
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bioRxiv - Genetics 2020Quote: ... RNA was extracted from samples using TRIzol and cDNA synthesized from 1 μg RNA template using PrimeScript™ RT reagent Kit with gDNA Eraser (TAKARA BIO INC). The sequences of primers used for amplification are shown in Table S6 ...
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bioRxiv - Biochemistry 2021Quote: ... In-fusion cloning was used for the ligation of sequence fragments to vector with the In-fusion HD Cloning Kit (Clontech Laboratories, Inc, US). Site-directed mutations were accomplished according to the protocol provided in a Mut Express II Fast Mutagenesis kit V2 (Vazyme ...
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bioRxiv - Biochemistry 2021Quote: ... vector (including the N-terminal hexahistidine and thrombin tags) by In-Fusion cloning using the In-Fusion HD Cloning Kit (Takara Bio, Kusatsu, Japan), yielding the pBAD-yTrm5 vector ...
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bioRxiv - Biophysics 2020Quote: ... tinctorius Nav1.4 were determined by DNA gel extraction and sequencing after rapid amplification of cDNA ends (RACE) using the SMARTer® RACE 5’/3’ Kit (Takara Bio, USA) and internal primers designed from P ...
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bioRxiv - Cell Biology 2021Quote: ... we linearized the pnCS plasmid with NdeI/NheI and employed seamless cloning (In-Fusion HD Cloning Plus Kit from Takara Bio, Cat. # 638910) with the following primers ...
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bioRxiv - Cancer Biology 2020Quote: ... and bound to strand-specific adaptors following the manufacturer’s protocol (SMARTer Stranded Total RNA Sample Prep Kit, Takara Bio, Mountain View, CA, USA). cDNA libraries were assayed for quality control by Bioanalyzer (Agilent Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... PCR amplification using proofreading DNA polymerases (Phusion HF, New England Biolab, Ipswich, MA) and In-Fusion HD Cloning Kit (Clontech, Mountain View, CA) or NEBuilder HiFi DNA Assembly Cloning Kits (New England Biolab) ...
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bioRxiv - Plant Biology 2021Quote: ... Reverse transcription was then carried out using total RNA and oligo-dT primers to synthesize the first strand cDNA using the PrimeScript One-Step RT-PCR Kit (Ver.2, Takara Biotechnology, Dalian, China) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: Total RNA (1 μg) was reverse transcribed to cDNA using the PrimeScriptTM RT reagent kit with gDNA Eraser (Cat#: RR047A, TaKaRa Bio, Shiga, Japan). qRT–PCR was performed using a LightCycler 480 II instrument (Roche Molecular Systems ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was synthesized from total RNA of each culture sample using the PrimeScript II 1st strand cDNA Synthesis Kit (Takara Bio, Shiga, Japan) with random hexamers to yield a total volume of 20 μL ...
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bioRxiv - Immunology 2020Quote: ... the 5×PrimeSTAR® Buffer (Mg2+ plus) in the kit was replaced with the 2 × PrimeSTAR® GC Buffer (Mg2+ plus) (Takara, Japan). The first PCR program was as follows ...
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bioRxiv - Immunology 2021Quote: ... T cell receptor sequencing libraries were prepared with the SMARTer Human TCR α/β Profiling Kit (catalog number 635015, Takara Bio USA, Inc.) according to manufacturer’s instructions with the exception of excluding the third and fourth bead size selection steps listed in Table 3 of the kit manual ...
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bioRxiv - Microbiology 2021Quote: ... for 15 min and then harvested for quantification of INF α mRNA using the One-step SYBR Prime script RT-PCR kit (TaKaRa, Dalian, China) according to the manufacturer’s protocol.Briefly the total RNA was isolated from TNF-α-treated with Trizol reagent (Takara Bio ...
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bioRxiv - Genetics 2019Quote: ... and the donor sequence, was sub-cloned and inserted into the vector (pX601, addgene #61591) using a DNA ligation kit (Clontech, Mountain View, CA). A plasmid for mutation replacement genome editing was constructed as described previously15 ...
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bioRxiv - Genetics 2019Quote: ... gRNA-1 target site or gRNA-4 target site and the donor sequence, were sub-cloned and inserted into the vector (pX601, addgene #61591) using a DNA ligation kit (Clontech, Mountain View, CA). To avoid repeated cleavage after mutation replacement ...
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bioRxiv - Molecular Biology 2022Quote: ... Finally RNA was isolated from the eluates by phenol-chloroform-Isoamyl alcohol (25:24:1) pH 4.5 extraction and cDNA was prepared using 1st strand cDNA synthesis kit (Takara Bio Inc., Shiga, Japan) as described in above RNA analysis section ...
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bioRxiv - Cancer Biology 2022Quote: ... cDNA synthesis and library preparation were performed in the UNMCCC ATG Shared Resource using the SMARTer Universal Low Input RNA Kit for Sequencing (Clontech, San Jose, CA) and the Ion Plus Fragment Library Kit (Life Technologies ...
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bioRxiv - Evolutionary Biology 2022Quote: ... with 0.1 µL of Taq Polymerase 5 U.µL-1 (TaKaRa Ex Taq® Kit MgCl2 Free Buffer) (TaKaRa Ex Taq, TaKaRa Bio, Shiga, Japan), 2.5 µL PCR Buffer 10X ...
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bioRxiv - Molecular Biology 2022Quote: ... the 5′ and 3′’ untranslated regions (UTRs) of the virus were determined using a SMARTer®RACE 5′/3′ Kit (Takara Bio, USA). Viral RNA isolated in August ...
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bioRxiv - Cell Biology 2022Quote: ... cDNA was reverse transcribed from 500 ng of total RNA with hexamers using a PrimeScript RT reagent kit (Takara Bio, Inc., Kusatsu, Japan). qPCR was then performed in a 25 μl reaction using TB Green Premix Ex Taq II reagent (Tli RNaseH Plus ...
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bioRxiv - Cell Biology 2022Quote: The collected embryonic ICM and polar TE (as described above) were used for RNA-seq library preparation using the SMART-Seq HT Kit (Takara Bio USA, Inc.) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... Extracted RNA from each juvenile (as described in Methods S1) was converted to cDNA using PrimeScript RT-PCR Kit (Takara Bio, Shiga, Japan). The efficiency and specificity of the designed primers was tested through PCR using the GoTaq Green Master kit (Promega ...
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bioRxiv - Plant Biology 2022Quote: ... as described by the manufacturer’s instructions and complementary DNA was synthesized for real-time quantitative polymerase chain reaction (RT-qPCR; Primescript RT Reagent Kit, Takara Bio, Kusatsu, Japan). RT-qPCR analysis was performed for the gene PR1 (AT2G14610 ...
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bioRxiv - Plant Biology 2022Quote: ... Total RNA was isolated using Quick-RNA miniprep kit (ZymoResearch, CA, USA) and 1 μg of RNA was used to generate cDNA using SMART MMLV reverse transcriptase (Takara Bio USA, Inc.). Transcript abundance of an ABA biosynthesis gene ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: The cDNA was synthesized using 1 µg of RNA from each treatment using the PrimeScript™ RT reagent Kit with gDNA Eraser (TaKaRa Dalian, China). RT-qPCR was performed on a Bio-Rad CFX96 (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... Approximately 1 µg of total RNA was used for reverse transcription using the PrimeScript™ RT reagent kit with gDNA Eraser (Takara, Beijing, China). After the transcribed cDNAs were 5-fold diluted ...
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bioRxiv - Immunology 2022Quote: RNA was extracted from LNs and used for RT-qPCR using a Prime Script™ II Strand cDNA Synthesis kit (Takara, Dalian, China), in accordance with the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... The amplified PCR products and pJM101/L1.3FLAG plasmid DNA then were digested with NotI and AgeI and ligated using the DNA Ligation Kit Mighty Mix (TaKaRa Bio, Shiga, Japan) at 16°C for 30 minutes ...