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Citations for Takara Bio :
4651 - 4700 of 5255 citations for Rat Myristoylated Alanine Rich C Kinase Substrate MARCKS ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... Complementary oligonucleotides with overhangs were annealed and cloned into the BbsI-digested U6b vector using a DNA ligation kit (Takara, 6023).
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bioRxiv - Neuroscience 2023Quote: ... Libraries were then constructed using the SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian (Takara Bio Inc., Shiga, Japan), which features integration of unique molecular identifiers (UMIs) ...
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bioRxiv - Microbiology 2023Quote: ... RT-qPCR was performed in a single-step using the One-Step TB Green PrimeScript RT-PCR Kit II (Takara Bio). The HCoV-OC43 nucleocapsid gene was amplified with the following primers ...
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bioRxiv - Immunology 2023Quote: ... Lactate dehydrogenase (LDH) in the supernatants from either cell type was quantified using the LDH Cytotoxicity Detection kit (TaKaRa or Roche) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: was used to carry out reverse transcription (RT) reaction to obtain cDNA following the instruction of PrimeScript RT Reagent Kit (Takara, Japan). RT primers are specific to midRs ...
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bioRxiv - Microbiology 2023Quote: ... from pRM1071 was cloned into NcoI-HindIII-digested pHM1552 (Miyazaki et al., 2022) using In-Fusion HD Cloning Kit (Takara bio).
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bioRxiv - Genomics 2023Quote: ... RNA (1 ng) was used for cDNA synthesis and amplification using the SMART-Seq HT RNA-Seq library amplification kit (Takara Bio). According to the manufacturer’s recommendations ...
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bioRxiv - Plant Biology 2023Quote: ... each egg cell sample was transferred to a lysis buffer supplied in the SMART-Seq HT kit (Takara Bio, Shiga, Japan). The lysates were immediately used for cDNA synthesis or frozen in liquid nitrogen and stored at -80 °C ...
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bioRxiv - Neuroscience 2023Quote: ... total RNAs were reverse transcribed to complementary DNA (cDNA) using both oligo dT and random primers with PrimeScript RT Reagent Kit (Takara, #RR037A) according to manufacturer’s instructions in the presence of a synthetic external non-homologous poly(A ...
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bioRxiv - Neuroscience 2023Quote: ... Two micrograms of the RNA were subjected to reverse transcription with random hexamers and the PrimeScript™ 1st strand cDNA Synthesis Kit (TaKaRa). For semi-quantitative PCR ...
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bioRxiv - Cell Biology 2023Quote: ... and constructs were isolated from 50 ml bacterial cultures using a plasmid DNA extraction kit (Takara Bio USA, Inc. NucleoSpin Plasmid). DNA concentrations were measured by a NanoDrop spectrophotometer ...
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bioRxiv - Plant Biology 2023Quote: ... which was then reverse-transcribed into first-strand cDNA using the Prime-Script-TM RT Reagent Kit (RR036, Takara, Kyoto, Japan). The quantitative real-time polymerase chain reaction was performed using the ABI 7300 sequencer and SYBR Premix Ex Taq-TM (RR420 ...
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bioRxiv - Pathology 2023Quote: ... The cDNA was prepared from the extracted RNAs with oligo (dT) and random primers using the PrimeScript RT reagent kit (TaKaRa, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... the correct full-length sequences were determined for the laboratory strains using a SMARTer RACE 5’/3’ kit (Takara, Shiga, Japan). These sequences were aligned by ClustalW ...
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bioRxiv - Molecular Biology 2023Quote: ... 500 ng of the total RNA was used for cDNA synthesis using the Prime Script 1st Strand cDNA Synthesis Kit (TaKaRa, 6110A). Random 6-mer primers (0.3 µL ...
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bioRxiv - Molecular Biology 2023Quote: ... Sequencing libraries were created following the protocol described in the SMARTer® smRNA-Seq Kit for Illumina user manual (Takara Bio).
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bioRxiv - Plant Biology 2024Quote: ... the 5 μl samples were used as input into the SMART-Seq v4 Ultra Low Input RNA Kit (Takara Bio USA) (for parasitic J3 library generation ...
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bioRxiv - Zoology 2024Quote: ... RNA was extracted using Isogen-LS (Nippon Gene) and RT-qPCR was performed using the One-Step PrimeScript RT-PCR Kit (Takara Bio) on a 7500 Real-Time RT-PCR System (Applied Biosystems) ...
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bioRxiv - Microbiology 2023Quote: Total DNA was extracted from all the MBS and soil samples using the NucleoSpin Soil DNA extraction kit (Takara Bio, USA), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... linearized chimeric H3 HA sequence was gel purified using the NucleoSpin Gel and PCR Clean-up kit (Takara, Cat. No. 740609.5) and then purified using Ampure XP beads (Beckman Coulter ...
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bioRxiv - Genomics 2023Quote: ... The library inserts from last step were ligated to the linearized STARR-seq vectors using In-Fusion HD Cloning Kit (Clontech 639650) following the manufactory’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... We then synthesized these sequences as G-blocks (Integrated DNA Technologies) and cloned them using Infusion kit (Takara biosciences catalog # 638948) into pSMART-HC-Kan (Lucigen ...
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bioRxiv - Cancer Biology 2023Quote: ... Three RNA lysis aliquots of the LC6 sample were processed and library preparation completed on the 3 aliquots with SMART-Seq® HT Kit (Takara) and Illumina Nextera reagents for tagmentation ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA-seq libraries were prepared from these samples using the SMARTer® Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian (Takara) kit according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was extracted from cells and reverse-transcribed to cDNA using the PrimeScript 1st Strand cDNA Synthesis Kit (TaKaRa Bio). cDNA was used as a template for real-time qPCR analysis on CFX96 Touch (Bio-Rad ...
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bioRxiv - Biophysics 2023Quote: Broccoli RNA template sequences were ordered as dsDNA gBlock as above and inserted into a linearized pRZ plasmid using infusion cloning kit (Takara Bio) and transformed into Stellar cells following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... complementary DNA (cDNA) was synthesized from the total RNA samples using the TaKaRa PrimeScript 1st strand cDNA Synthesis Kit (TaKaRa, 6110A).
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bioRxiv - Cancer Biology 2023Quote: Reverse Transcriptase PCR and Real-Time PCR Cells cultured in either 12- or 24-well plates were washed twice with cold PBS and harvested using a TaKaRa MiniBEST universal RNA extraction kit (Takara, Japan). RNA was purified using the same kit according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... A quantity of RNA in the range 200 – 500 ng was used for cDNA synthesis using the PrimeScript RT-PCR Kit (Takara-Bio). Quantitative PCR was then performed using Taqman Fast Advanced MasterMix with Universal Probe Library Probes (Roche ...
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bioRxiv - Microbiology 2023Quote: ... 90 μl of RNase free water was added and then 2.5 μl of diluted sample was used for real-time RT-PCR according to the manufacturer’s protocol with One step TB green PrimeScript PLUS RT-PCR Kit (Takara, Cat# RR096A) and primers for Nucleocapsid (N ...
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bioRxiv - Microbiology 2023Quote: ... This clone was digested with NsiI and BglII to remove the CMV promoter sequence and was combined with the pSV010(-) SV40 promoter fragment using the Infusion® HD Cloning kit (Clontech) such that the pSV010(- ...
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bioRxiv - Molecular Biology 2023Quote: ... The RNA was then reverse-transcribed into complementary DNA (cDNA) using the RR036A PrimeScript™ RT Reagent Kit (Perfect Real Time) supplied by TaKaRa. The amplification products were quantified using SYBR Green (Vazyme) ...
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bioRxiv - Immunology 2023Quote: ... reverse transcription and cDNA pre-amplification were performed using the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech/Takara). cDNA was harvested and quantified with the Bioanalyzer DNA High-Sensitivity kit (Agilent Technologies) ...
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bioRxiv - Physiology 2023Quote: ... Up to 30 ng of post-Immunoprecipitation dsDNA as measured by Invitrogen™ Qubit™ highsensitivity spectrofluorometric measurement was made into Illumina-compatible NGS libraries using the ThruPLEX® DNA-Seq kit from Takara Bio Inc ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... cDNA was synthesized from 100 µg of total RNA by Prime ScriptTM RT reagent kit with gDNA Eraser (Takara, Tokyo, Japan). Each cDNA sample was amplified for the gene of interest and β-actin in a 15 µL reaction volume TB GreenTM Premix Ex Taq™ II (Takara ...
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bioRxiv - Evolutionary Biology 2023Quote: Complete cDNA sequences of taste receptors were obtained using a SMARTer 5’/3’ kit for RACE PCR to obtain 5’ sequence information missing from reference transcriptome (Takara, #634858). Briefly ...
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bioRxiv - Immunology 2023Quote: ... This RNA was used as input to the Mir-X™ miRNA qRT-PCR TB Green® Kit (Takara cat# 638316) to generate miRNA cDNA ...
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bioRxiv - Immunology 2023Quote: ... The expression vectors for the S variants with multiple amino acid changes or deletions were generated using the In-Fusion HD cloning kit (Takara Bio). The pcDNA3.1-hACE2 used to express human ACE2 (hACE2 ...
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bioRxiv - Synthetic Biology 2024Quote: ... 23S rRNA mutations were introduced to the pLK35 plasmid using the corresponding primer set (Supplementary Table 5) and the In-Fusion Cloning kit (Takara Bio). All sequences were confirmed with full plasmid nanopore sequencing (Plasmidsaurus and Elim Bio).
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bioRxiv - Immunology 2024Quote: ... A total of 1 μg of total RNA was reverse-transcribed with random primers using the PrimeScript™ 1st strand cDNA Synthesis Kit (TaKaRa) according to the manufacturer’s protocols ...
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bioRxiv - Microbiology 2023Quote: ... the amplified fragment was incorporated into the Bam HI site of pHSG396 using the In-Fusion HD cloning kit (TAKARA, Japan), resulting in the generation of pHSG396-ptrA.
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bioRxiv - Cell Biology 2024Quote: ... GS linker human IgG1 FC region from pcDNA3-sACE2v2-Fc (IgG1) and PGK promoter puromycin resistance region cloned into lenti CAG-FLAG-dCas9-VPR using infusion cloning kit (Takara, 638947). After packaging the lentivirus ...
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bioRxiv - Developmental Biology 2024Quote: ... The fragment was then inserted in the pCAGEN-IRES-tomato with the In-Fusion® HD Cloning Kit (639649, Takara bio) according to manufacturer recommendations to generate pCAGEN-ATP1A3-WT-IRES-Tomato (named ATP1A3-WT) ...
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bioRxiv - Neuroscience 2024Quote: ... Titers were obtained on freeze-thawed aliquots using the qPCR-based TaKaRa lentivirus titration kit (#631235, Takara Bio, Kusatsu, Shiga, Japan). Minimum titers for all in vivo experiments was 1×1011 genome copies/mL ...
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bioRxiv - Immunology 2024Quote: ... The damaged lung areas of the infected mice and the corresponding lung areas of the uninfected control mice were excised to isolate total RNAs using TaKaRa MiniBEST Universal RNA Extraction Kit (TaKaRa, 9767), which were reverse transcribed into cDNAs using the RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2024Quote: ... The extracted RNA was quantified by Nanodrop and 500 ng of it was converted into cDNA using a PrimeScript™ RT reagent Kit (Takara). Quantitative RT-PCR (qRT-PCR ...
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bioRxiv - Synthetic Biology 2024Quote: ... the rRNA-depleted and fragmented total RNA fraction was converted to sequencing libraries using the SMARTer® smRNA-Seq Kit for Illumina (Catalog number #635030) from Takara Bio USA ...
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bioRxiv - Cancer Biology 2024Quote: ... MCF-7 and ErbB2-overexpressing MCF-7 cells were routinely tested using the CycleavePCR Mycoplasma Detection Kit (Takara bio, CY-232). None of the cell lines were contaminated with mycoplasma.
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bioRxiv - Immunology 2024Quote: ... Samples were then processed for the preparation of libraries using the SMARTer Stranded Total RNA Sample Prep Kit (Takara Bio USA) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... and the amplicons were then ligated into a pUAST-attB vector digested by EcoRI/NotI using In-Fusion HD Cloning Kit (Takara, 639649). The landing site for each construct was {3xP3-RFP.attP}ZH-86Fb ...